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  1. Article ; Online: Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome.

    Gu Urban, Gucci Jijuan / Friedman, Mikaela / Ren, Ping / Törn, Carina / Fex, Malin / Hampe, Christiane S / Lernmark, Åke / Landegren, Ulf / Kamali-Moghaddam, Masood

    Scientific reports

    2015  Volume 5, Page(s) 11196

    Abstract: Glutamic acid decarboxylase 65 (GAD65) and autoantibodies specific for GAD65 (GADA) are associated with autoimmune diseases including Stiff Person Syndrome (SPS) and Type 1 diabetes (T1D). GADA is recognized as a biomarker of value for clinical diagnosis ...

    Abstract Glutamic acid decarboxylase 65 (GAD65) and autoantibodies specific for GAD65 (GADA) are associated with autoimmune diseases including Stiff Person Syndrome (SPS) and Type 1 diabetes (T1D). GADA is recognized as a biomarker of value for clinical diagnosis and prognostication in these diseases. Nonetheless, it remains medically interesting to develop sensitive and specific assays to detect GAD65 preceding GADA emergence, and to monitor GADA-GAD65 immune complexes in blood samples. In the present study, we developed a highly sensitive proximity ligation assay to measure serum GAD65. This novel assay allowed detection of as little as 0.65 pg/ml GAD65. We were also able to detect immune complexes involving GAD65 and GADA. Both free GAD65 and GAD65-GADA levels were significantly higher in serum samples from SPS patients compared to healthy controls. The proximity ligation assays applied for detection of GAD65 and its immune complexes may thus enable improved diagnosis and better understanding of SPS.
    MeSH term(s) Adult ; Aged ; Antigen-Antibody Complex/blood ; Antigen-Antibody Complex/immunology ; Autoantibodies/immunology ; Case-Control Studies ; Female ; Glutamate Decarboxylase/blood ; Humans ; Immunoassay ; Male ; Middle Aged ; Peptide Fragments/blood ; Stiff-Person Syndrome/blood ; Stiff-Person Syndrome/immunology
    Chemical Substances Antigen-Antibody Complex ; Autoantibodies ; Peptide Fragments ; glutamate decarboxylase 65 (202-221) (EC 4.1.1.-) ; Glutamate Decarboxylase (EC 4.1.1.15)
    Language English
    Publishing date 2015-06-16
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/srep11196
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Integrative Personal Omics Profiles during Periods of Weight Gain and Loss.

    Piening, Brian D / Zhou, Wenyu / Contrepois, Kévin / Röst, Hannes / Gu Urban, Gucci Jijuan / Mishra, Tejaswini / Hanson, Blake M / Bautista, Eddy J / Leopold, Shana / Yeh, Christine Y / Spakowicz, Daniel / Banerjee, Imon / Chen, Cynthia / Kukurba, Kimberly / Perelman, Dalia / Craig, Colleen / Colbert, Elizabeth / Salins, Denis / Rego, Shannon /
    Lee, Sunjae / Zhang, Cheng / Wheeler, Jessica / Sailani, M Reza / Liang, Liang / Abbott, Charles / Gerstein, Mark / Mardinoglu, Adil / Smith, Ulf / Rubin, Daniel L / Pitteri, Sharon / Sodergren, Erica / McLaughlin, Tracey L / Weinstock, George M / Snyder, Michael P

    Cell systems

    2018  Volume 6, Issue 2, Page(s) 157–170.e8

    Abstract: Advances in omics technologies now allow an unprecedented level of phenotyping for human diseases, including obesity, in which individual responses to excess weight are heterogeneous and unpredictable. To aid the development of better understanding of ... ...

    Abstract Advances in omics technologies now allow an unprecedented level of phenotyping for human diseases, including obesity, in which individual responses to excess weight are heterogeneous and unpredictable. To aid the development of better understanding of these phenotypes, we performed a controlled longitudinal weight perturbation study combining multiple omics strategies (genomics, transcriptomics, multiple proteomics assays, metabolomics, and microbiomics) during periods of weight gain and loss in humans. Results demonstrated that: (1) weight gain is associated with the activation of strong inflammatory and hypertrophic cardiomyopathy signatures in blood; (2) although weight loss reverses some changes, a number of signatures persist, indicative of long-term physiologic changes; (3) we observed omics signatures associated with insulin resistance that may serve as novel diagnostics; (4) specific biomolecules were highly individualized and stable in response to perturbations, potentially representing stable personalized markers. Most data are available open access and serve as a valuable resource for the community.
    MeSH term(s) Adult ; Biomarkers/blood ; Genomics/methods ; Humans ; Insulin Resistance/genetics ; Male ; Metabolomics/methods ; Obesity/genetics ; Precision Medicine/methods ; Proteomics/methods ; Weight Gain/genetics ; Weight Loss/genetics
    Chemical Substances Biomarkers
    Language English
    Publishing date 2018-01-17
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2854138-8
    ISSN 2405-4720 ; 2405-4712
    ISSN (online) 2405-4720
    ISSN 2405-4712
    DOI 10.1016/j.cels.2017.12.013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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