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  1. Article: Isolated case of platelet satellitism around white blood cells and phagocytosis by neutrophils and monocytes.

    Lorubbio, Maria / Ognibene, Agostino

    Practical laboratory medicine

    2022  Volume 29, Page(s) e00264

    Abstract: Introduction: Rarely, platelets can interact with other blood elements, forming platelet aggregates. This paper presents an isolated case of platelet satellitism around neutrophils, lymphocytes and monocytes with platelet phagocytosis by both ... ...

    Abstract Introduction: Rarely, platelets can interact with other blood elements, forming platelet aggregates. This paper presents an isolated case of platelet satellitism around neutrophils, lymphocytes and monocytes with platelet phagocytosis by both neutrophils and monocytes.
    Case presentation: The subject was an 89-year-old woman with breast cancer on anti-estrogenic hormone cancer therapy. Whole blood sample collected in a tube with EDTA (Ethylenediaminetetra-acetic acid) anticoagulant was analysed within 4 hours, using an XN haematology analyser (Sysmex). The CBC (complete blood count) presented the following results: WBC (White blood cell) 4.0 x 10
    Conclusion: This manuscript describes the platelet satellitism around neutrophils, lymphocytes and monocytes and the interesting, very rare and singular phenomenon of platelet phagocytosis by not only neutrophils but also monocytes.
    Language English
    Publishing date 2022-01-14
    Publishing country Netherlands
    Document type Case Reports
    ZDB-ID 2834973-8
    ISSN 2352-5517
    ISSN 2352-5517
    DOI 10.1016/j.plabm.2022.e00264
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  2. Article ; Online: Isolated case of platelet satellitism around white blood cells and phagocytosis by neutrophils and monocytes

    Maria Lorubbio / Agostino Ognibene

    Practical Laboratory Medicine, Vol 29, Iss , Pp e00264- (2022)

    2022  

    Abstract: Introduction: Rarely, platelets can interact with other blood elements, forming platelet aggregates. This paper presents an isolated case of platelet satellitism around neutrophils, lymphocytes and monocytes with platelet phagocytosis by both neutrophils ...

    Abstract Introduction: Rarely, platelets can interact with other blood elements, forming platelet aggregates. This paper presents an isolated case of platelet satellitism around neutrophils, lymphocytes and monocytes with platelet phagocytosis by both neutrophils and monocytes. Case presentation: The subject was an 89-year-old woman with breast cancer on anti-estrogenic hormone cancer therapy. Whole blood sample collected in a tube with EDTA (Ethylenediaminetetra-acetic acid) anticoagulant was analysed within 4 hours, using an XN haematology analyser (Sysmex). The CBC (complete blood count) presented the following results: WBC (White blood cell) 4.0 x 109/L, RBC (Red blood cell) 3.58 x 1012/L, haemoglobin 116 g/L, haematocrit 34.9%, MCV (Mean corpuscular volume) 97.5 fL, MCH (Mean corpuscular haemoglobin) 32.5 pg, MCHC (Mean corpuscular haemoglobin concentration) 33.2 g ⁄dL, RDW (Red blood cell distribution width) 14.6% and PLT (Platelet) 136. Conclusion: This manuscript describes the platelet satellitism around neutrophils, lymphocytes and monocytes and the interesting, very rare and singular phenomenon of platelet phagocytosis by not only neutrophils but also monocytes.
    Keywords Platelet satellitism ; Platelet phagocytosis ; Isolated pseudothrombocytopenia ; White blood cells ; Microscopic evaluation ; Medicine (General) ; R5-920 ; Chemistry ; QD1-999
    Subject code 610 ; 630
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
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  3. Article ; Online: Experience of Uncommon EGFR Mutation in Lung Scheme Quality Program: Discussing Risks and Opportunities for the Improvement of Laboratory Response.

    Pancrazzi, Alessandro / Ognibene, Agostino / Moncada, Alice / Torre, Valerio

    Global medical genetics

    2021  Volume 8, Issue 4, Page(s) 171–175

    Abstract: ... ...

    Abstract Background
    Language English
    Publishing date 2021-07-22
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 3009997-3
    ISSN 2699-9404 ; 2699-9404
    ISSN (online) 2699-9404
    ISSN 2699-9404
    DOI 10.1055/s-0041-1732481
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  4. Article: Evaluation of Stability and Accuracy Compared to the Westergren Method of ESR Samples Analyzed at VES-MATIC 5.

    Lorubbio, Maria / Diamanti, Daniela / Ghiandai, Alessandro / Pieroni, Carolina / Bonini, Donatella / Pettinari, Massimiliano / Gorini, Gabriele / Bassi, Stefania / Meloni, Paola / Ognibene, Agostino

    Diagnostics (Basel, Switzerland)

    2024  Volume 14, Issue 5

    Abstract: The Erythrocyte Sedimentation Rate (ESR) is a diagnostic estimator of systemic inflammation as a reflection of acute phase proteins circulating in the blood. The purpose of this manuscript is to evaluate the blood stability at room temperature (RT) and ... ...

    Abstract The Erythrocyte Sedimentation Rate (ESR) is a diagnostic estimator of systemic inflammation as a reflection of acute phase proteins circulating in the blood. The purpose of this manuscript is to evaluate the blood stability at room temperature (RT) and at 4 °C to avoid ESR diagnostic errors, as well as the accuracy of the VES-MATIC 5 analyzer. The ESR stability evaluation at RT for 24 h (4 h "T1", 6 h "T2", 8 h "T3", 10 h "T4", 24 h "T5") and at 4 °C (24 h, 36 h, 48 h) was carried out using 635 total samples, starting with T0 (2 h of venipuncture). For method comparison, 164 patients were analyzed using VES-MATIC 5 and then the Westergren reference method. The sample at RT is established by a significant gradual decrease in correlation R = 0.99 (T0 vs. T1), R = 0.97 (T0 vs. T2), R = 0.92 (T0 vs. T3), R = 0.87 (T0 vs. T4), and R = 0.40 (T0 vs. T5). The stability at 4 °C after 24 h, 36 h, and 48 h showed a regression of R = 0.99, R = 0.97, and R = 0.95, respectively. Therefore, ESR measurements on RT samples beyond 6 h after collection cannot be carried out, but the ESR can be measured until 36 h for samples stored at 4 °C. Moreover, the VES-MATIC 5 accuracy performance compared to the Westergren method (R = 0.96) is confirmed.
    Language English
    Publishing date 2024-03-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662336-5
    ISSN 2075-4418
    ISSN 2075-4418
    DOI 10.3390/diagnostics14050557
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  5. Article: Identification of a Novel Non-V600E BRAF Mutation in Papillary Thyroid Cancer.

    Capezzone, Marco / Rossi, Maja / Macerola, Elisabetta / Cantara, Silvia / Pepe, Francesco / Morabito, Eugenia Maria / Dalmazio, Gilda / Bardi, Sara / Ognibene, Agostino / Alessandri, Massimo / Materazzi, Gabriele / De Napoli, Luigi / Cirianni, Michele / Torregrossa, Liborio

    Case reports in endocrinology

    2024  Volume 2024, Page(s) 6621510

    Abstract: Papillary thyroid cancer (PTC) is a common endocrine malignancy, and its incidence is reported to be constantly increasing. BRAF mutation is detected in approximately 44% of PTCs, and the most common BRAF mutation is thymine (T) to adenine (A) missense ... ...

    Abstract Papillary thyroid cancer (PTC) is a common endocrine malignancy, and its incidence is reported to be constantly increasing. BRAF mutation is detected in approximately 44% of PTCs, and the most common BRAF mutation is thymine (T) to adenine (A) missense mutation in nucleotide 1796 (T1796A, V600E). Although BRAFV600E represents 95% of all BRAF mutations, uncommon BRAF mutations have been reported in thyroid carcinomas and represent an alternative mechanism of BRAF activation with unclear clinical significance. We report a novel non-V600E mutation (c.1799_1812delinsAT, p.V600_W604delinsD), identified preoperatively with next-generation sequencing (NGS) on the material obtained with fine-needle aspiration cytology (FNAC) performed on a thyroid nodule cytologically suspicious for malignancy in a 35-year-old male patient. The presence of this new variant of BRAF mutation was subsequently confirmed in the postoperative phase by direct Sanger sequencing. In conclusion, we report a new non-V600E variant previously undetected in papillary thyroid cancer. In addition, this case report shows that the NGS technique on cytological tissue allows to detect the presence of rare mutations, thus increasing the diagnostic specificity of molecular analysis.
    Language English
    Publishing date 2024-03-19
    Publishing country United States
    Document type Case Reports
    ZDB-ID 2627633-1
    ISSN 2090-651X ; 2090-6501
    ISSN (online) 2090-651X
    ISSN 2090-6501
    DOI 10.1155/2024/6621510
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  6. Article ; Online: Macro-aspartate aminotransferase in a healthy woman.

    Lorubbio, Maria / Ognibene, Agostino / Salvadori, Benedetta / Fanelli, Alessandra / Laffi, Giacomo

    Clinical and molecular hepatology

    2020  Volume 26, Issue 3, Page(s) 378–381

    MeSH term(s) Aspartate Aminotransferases/blood ; Aspartate Aminotransferases/chemistry ; Female ; Humans ; Macromolecular Substances/blood ; Macromolecular Substances/chemistry ; Middle Aged ; Polyethylene Glycols/chemistry
    Chemical Substances Macromolecular Substances ; Polyethylene Glycols (3WJQ0SDW1A) ; Aspartate Aminotransferases (EC 2.6.1.1)
    Language English
    Publishing date 2020-06-23
    Publishing country Korea (South)
    Document type Letter
    ZDB-ID 2672560-5
    ISSN 2287-285X ; 2287-2728
    ISSN (online) 2287-285X
    ISSN 2287-2728
    DOI 10.3350/cmh.2020.0069
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    In stock of ZB MED Cologne/Königswinter

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  7. Article ; Online: Midregional pro-adrenomedullin (MR-ProADM) reference values in serum.

    Lorubbio, Maria / Conti, Andrea A / Ognibene, Agostino

    Clinical biochemistry

    2018  Volume 53, Page(s) 173–174

    MeSH term(s) Adrenomedullin/blood ; Adult ; Female ; Humans ; Male ; Middle Aged ; Reference Values
    Chemical Substances Adrenomedullin (148498-78-6)
    Language English
    Publishing date 2018-03
    Publishing country United States
    Document type Letter ; Randomized Controlled Trial
    ZDB-ID 390372-2
    ISSN 1873-2933 ; 0009-9120
    ISSN (online) 1873-2933
    ISSN 0009-9120
    DOI 10.1016/j.clinbiochem.2018.01.003
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    In stock of ZB MED Cologne/Königswinter

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  8. Article: Experience of Uncommon EGFR Mutation in Lung Scheme Quality Program: Discussing Risks and Opportunities for the Improvement of Laboratory Response

    Pancrazzi, Alessandro / Ognibene, Agostino / Moncada, Alice / Torre, Valerio

    Global Medical Genetics

    2021  Volume 08, Issue 04, Page(s) 171–175

    Abstract: Background: The quality programs can be considered to be a valuable tool for global and individual growth. Each result, obtained by a single laboratory, contributes to define the standardization of the response. In the case of the uncommon epidermal ... ...

    Abstract Background: The quality programs can be considered to be a valuable tool for global and individual growth. Each result, obtained by a single laboratory, contributes to define the standardization of the response. In the case of the uncommon epidermal growth factor receptor (EGFR) mutations, the molecular result is sometimes difficult to interpret in terms of biological significance and therapy choosing. The standardization effort in the diagnostic lung setting also consists of active quality program participation.
    Materials and Methods: The quality control analysis, which is defined as a clinical case, was performed by the extraction of DNA from FFPE sections and by RT-PCR on the EGFR (exons 19, 20, 21), BRAF, and KRAS genes. The laboratory performed a validation sequencing of EGFR exon 20 with the help of the Sanger method.
    Results: The laboratory reported positivity for EGFR exon 20 insertions and negative results for BRAF and KRAS. The quality test finished with the redaction of a report containing the recommendation to consider the efficacy of therapy with tyrosine kinase inhibitors (TKI). This specific interpretation has determined poor performance judgment by the quality provider, which explained why most of these mutations are TKI-resistant.
    Conclusions: This experience provides an opportunity to reflect on the critical aspects of this diagnostic setting. The detection of some uncommon EGFR mutations should entail the mutation characterization, especially for the rare exon 20 insertions, of which are not classifiable as “resistant.” Moreover, this experience allows reflecting on the quality program design, mandatory actions for the laboratory, and routine activity in the oncologic multidisciplinary team.
    Keywords uncommon EGFR mutations ; TKIs ; quality programs
    Language English
    Publishing date 2021-07-22
    Publisher Georg Thieme Verlag KG
    Publishing place Stuttgart ; New York
    Document type Article
    ZDB-ID 3009997-3
    ISSN 2699-9404 ; 2699-9404
    ISSN (online) 2699-9404
    ISSN 2699-9404
    DOI 10.1055/s-0041-1732481
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  9. Article ; Online: The role of Monocyte Distribution Width (MDW) in the prognosis and monitoring of COVID-19 patients.

    Lorubbio, Maria / Tacconi, Danilo / Iannelli, Giovanni / Feri, Marco / Scala, Raffaele / Montemerani, Sara / Mandò, Massimo / Ognibene, Agostino

    Clinical biochemistry

    2022  Volume 103, Page(s) 29–31

    Abstract: The new parameter derived from the standard deviation of the monocyte distribution width (MDW) has shown a good diagnostic efficacy in COVID-19 patients. In this study, we propose MDW as a prognostic and monitoring parameter in patients with severe forms ...

    Abstract The new parameter derived from the standard deviation of the monocyte distribution width (MDW) has shown a good diagnostic efficacy in COVID-19 patients. In this study, we propose MDW as a prognostic and monitoring parameter in patients with severe forms of COVID-19. Sixty SARS-CoV-2-positive patients admitted to the San Donato Hospital in Arezzo were enrolled. A blood sample taken to measure the complete blood count was used for the determination of MDW using a UniCel DxH 900 instrument (Beckman Coulter). For each patient, a mean of 6 ± 2 measurements of MDW were taken. The difference between the last and first MDW results was reported as the ΔMDW variable. The ΔMDW and age were significantly correlated to the outcome. In non-survivors patients, the difference in the mean of the MDW between the first and other points was not significant, while in survivors, the first point was higher than the other points (p < 0.005), with the exception of the mean of the second point (p-value = NS). The ΔMDW area under the curve (AUC) was 0.84, and with a cut-off lower than 0.00 the sensitivity and specificity were 88% and 81%, respectively. The most important result of this study is the ΔMDW calculated on the basis of the difference between the first and third measurement, after approximately the 5-7th day of hospitalisation. A ΔMDW less than one was indicative of an unfavourable prognosis. The data reported suggest that MDW could be used to support monitoring and surveillance, alongside other tests such as procalcitonin, in critically ill patients in the ICU.
    MeSH term(s) Biomarkers ; COVID-19/diagnosis ; Humans ; Monocytes ; Prognosis ; ROC Curve ; SARS-CoV-2 ; Sepsis
    Chemical Substances Biomarkers
    Language English
    Publishing date 2022-02-16
    Publishing country United States
    Document type Journal Article
    ZDB-ID 390372-2
    ISSN 1873-2933 ; 0009-9120
    ISSN (online) 1873-2933
    ISSN 0009-9120
    DOI 10.1016/j.clinbiochem.2022.02.007
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  10. Article: Novel Biomarkers for Early Detection of Acute Kidney Injury and Prediction of Long-Term Kidney Function Decline after Partial Nephrectomy.

    Allinovi, Marco / Sessa, Francesco / Villa, Gianluca / Cocci, Andrea / Innocenti, Samantha / Zanazzi, Maria / Tofani, Lorenzo / Paparella, Laura / Curi, Dritan / Cirami, Calogero Lino / Campi, Riccardo / Mari, Andrea / Ognibene, Agostino / Lorubbio, Maria / Fanelli, Alessandra / Romagnoli, Stefano / Romagnani, Paola / Minervini, Andrea

    Biomedicines

    2023  Volume 11, Issue 4

    Abstract: Background: Identifying acute kidney injury (AKI) within few hours of onset is certainly helpful. However, early prediction of a long-term eGFR decline may be an even more important goal. Our aim was to identify and compare serum [creatinine, kineticGFR, ...

    Abstract Background: Identifying acute kidney injury (AKI) within few hours of onset is certainly helpful. However, early prediction of a long-term eGFR decline may be an even more important goal. Our aim was to identify and compare serum [creatinine, kineticGFR, cystatin C, neutrophil gelatinase-associated lipocalin (NGAL)] and urinary (NephroCheck, NGAL, proteinuria, albuminuria, acantocytes at urinary sediment) predictors of AKI that might efficiently predict long-term GFR decline after robotic Nephron-Spearing Surgery (rNSS).
    Methods: Monocentric prospective observational study. Patients scheduled for rNSS for suspected localized Renal Cell Carcinoma from May 2017 to October 2017 were enrolled. Samples were collected preoperatively and postoperatively (timepoints: 4 h, 10 h, 24 h, 48 h), while kidney function was re-assessed up to 24 months.
    Results: 38 patients were included; 16 (42%) developed clinical AKI. The eGFR decline at 24 months was more pronounced after postoperative AKI (-20.75 vs. -7.20,
    Conclusions: NephroCheck and kineticGFR have emerged as promising noninvasive, accurate, and early biomarkers of postoperative AKI and long-term GFR decline after rNSS. Combining NephroCheck and kineticGFR in clinical practice would allow to identify high risk of postoperative AKI and long-term GFR decline as early as 10 h after surgery.
    Language English
    Publishing date 2023-03-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720867-9
    ISSN 2227-9059
    ISSN 2227-9059
    DOI 10.3390/biomedicines11041046
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