LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 15

Search options

  1. Article ; Online: A biochemiluminescent assay for rapid diagnosis of influenza.

    Lin, Xuexiang / Gu, Jia

    Clinical and experimental medicine

    2022  Volume 22, Issue 4, Page(s) 577–581

    Abstract: A biochemiluminescent assay of influenza diagnosis is presented. The assay diagnoses influenza based on detection of the influenza viral neuraminidase activity. An instrument designed for the assay is also reported. This assay solves the problem that ... ...

    Abstract A biochemiluminescent assay of influenza diagnosis is presented. The assay diagnoses influenza based on detection of the influenza viral neuraminidase activity. An instrument designed for the assay is also reported. This assay solves the problem that current influenza virus diagnosis assays are susceptible to virus mutation. A luciferase-based complex is synthesized as biochemiluminescent substrate. The substrate is cleaved to free luciferin with presence of influenza neuraminidase in specimen. Luciferase is oxidized to oxyluciferin with luciferin as catalyzer resulting in luminescence, which is proportional to the neuraminidase activity and measured by instrument. The instrument uses a photomultiplier tube as sensor, with 24 test channels. Fine optical arrangements enable the instrument with high sensitivity and accuracy. A total of 389 clinical specimens were collected to evaluate the performance of the assay in clinical settings. This assay had a sensitivity and specificity of 95.92% (95% confidence interval 91.38-98.12%) and 97.93% (95% confidence interval 95.26-99.11%), respectively, compared to the colloidal gold assay. As a biochemiluminscence assay, this assay is advantageous in sensitivity and specificity. It does not require any washing or separation steps, which makes the instrument simple in design and easy to operate or maintenance. The assay is suitable for the rapid diagnosis of influenza virus in point-of-care settings.
    MeSH term(s) Humans ; Influenza, Human/diagnosis ; Neuraminidase/genetics ; Sensitivity and Specificity ; Gold Colloid
    Chemical Substances Neuraminidase (EC 3.2.1.18) ; Gold Colloid
    Language English
    Publishing date 2022-01-10
    Publishing country Italy
    Document type Journal Article
    ZDB-ID 2053018-3
    ISSN 1591-9528 ; 1591-8890
    ISSN (online) 1591-9528
    ISSN 1591-8890
    DOI 10.1007/s10238-021-00778-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 5481: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Corrigendum to "Unraveling the mechanobiology of immune cells" [Curr Opin Biotechnol 66 (2020) 236-245].

    Zhang, Xuexiang / Kim, Tae-Hyung / Thauland, Timothy J / Li, Hongjun / Majedi, Fatemeh Sadat / Ly, Chau / Gu, Zhen / Butte, Manish J / Rowat, Amy C / Li, Song

    Current opinion in biotechnology

    2021  Volume 73, Page(s) 387–388

    Language English
    Publishing date 2021-12-09
    Publishing country England
    Document type Published Erratum
    ZDB-ID 1052045-4
    ISSN 1879-0429 ; 0958-1669
    ISSN (online) 1879-0429
    ISSN 0958-1669
    DOI 10.1016/j.copbio.2021.10.019
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 3071: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Elevated serum aspartate aminotransferase level identifies patients with coronavirus disease 2019 and predicts the length of hospital stay.

    Gu, Xuexiang / Li, Xiangyu / An, Xusheng / Yang, Shufeng / Wu, Shangnong / Yang, Xiaozhong / Wang, Honggang

    Journal of clinical laboratory analysis

    2020  Volume 34, Issue 7, Page(s) e23391

    Abstract: Background: Coronavirus disease 2019 (COVID-19) has become a worldwide public health emergency. This study aimed to investigate the clinical significance of liver blood tests in COVID-19 patients.: Methods: The analysis included clinical data of 23 ... ...

    Abstract Background: Coronavirus disease 2019 (COVID-19) has become a worldwide public health emergency. This study aimed to investigate the clinical significance of liver blood tests in COVID-19 patients.
    Methods: The analysis included clinical data of 23 patients with suspected COVID-19 and 66 patients with confirmed COVID-19 from January 25 to February 20, 2020. The relationship between liver blood test results, liver condition (HBsAb positive, HBcAb positive, and fatty liver disease), and duration of hospital stay among COVID-19 patients was analyzed.
    Results: The median hospital stay of COVID-19 patients was 6 days. Serum albumin (Alb) level was lower in patients with COVID-19 confirmed on admission than in patients with suspected COVID-19 (40.08 g/L vs 42.50 g/L, P = .016), while the level of aspartate aminotransferase (AST) was higher (23 U/L vs 18 U/L, P = .005). Abnormal results of liver blood tests in patients with COVID-19 included increased levels of alanine transaminase (ALT) (21.2%, 14 patients), AST (15.2%, 10 patients), and gamma-glutamyl transpeptidase (GGT) (22.7%, 15 patients). After 5-10 days of treatment, levels of Alb and AST in COVID-19 patients were significantly decreased (P < .001 and P = .027, respectively). Abnormal levels of Alb and AST in patients with COVID-19 were not associated with the liver condition (all P > .05). In addition, only levels of AST were positively correlated with the duration of hospital stay (r = .334, P = .007).
    Conclusion: Abnormal results of the liver blood test were found in COVID-19 patients. The COVID-19 patients on admission with the higher levels of AST might have longer hospital stays.
    MeSH term(s) Adult ; Aspartate Aminotransferases/blood ; Betacoronavirus/physiology ; COVID-19 ; Coronavirus Infections/blood ; Coronavirus Infections/enzymology ; Coronavirus Infections/therapy ; Coronavirus Infections/virology ; Female ; Humans ; Length of Stay ; Liver/virology ; Liver Function Tests ; Male ; Middle Aged ; Pandemics ; Pneumonia, Viral/blood ; Pneumonia, Viral/enzymology ; Pneumonia, Viral/therapy ; Pneumonia, Viral/virology ; SARS-CoV-2
    Chemical Substances Aspartate Aminotransferases (EC 2.6.1.1)
    Keywords covid19
    Language English
    Publishing date 2020-06-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 645095-7
    ISSN 1098-2825 ; 0887-8013
    ISSN (online) 1098-2825
    ISSN 0887-8013
    DOI 10.1002/jcla.23391
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2471: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Simultaneous stabilization of actin cytoskeleton in multiple nephron-specific cells protects the kidney from diverse injury

    Kamalika Mukherjee / Changkyu Gu / Agnieszka Collins / Marcel Mettlen / Beata Samelko / Mehmet M. Altintas / Yashwanth R. Sudhini / Xuexiang Wang / Richard Bouley / Dennis Brown / Bradley P. Pedro / Susan L. Bane / Vineet Gupta / Paul T. Brinkkoetter / Henning Hagmann / Jochen Reiser / Sanja Sever

    Nature Communications, Vol 13, Iss 1, Pp 1-

    2022  Volume 14

    Abstract: A common cellular manifestation for diverse kidney diseases is dysregulated actin cytoskeleton in distinct cell types that include glomerular podocytes and tubular epithelial cells. Here, authors pharmacologically activate dynamin and this results in ... ...

    Abstract A common cellular manifestation for diverse kidney diseases is dysregulated actin cytoskeleton in distinct cell types that include glomerular podocytes and tubular epithelial cells. Here, authors pharmacologically activate dynamin and this results in polymerization and crosslinking of actin filaments to establish the structural integrity of these cells, thus ameliorating disease phenotypes.
    Keywords Science ; Q
    Language English
    Publishing date 2022-05-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article ; Online: Simultaneous stabilization of actin cytoskeleton in multiple nephron-specific cells protects the kidney from diverse injury.

    Mukherjee, Kamalika / Gu, Changkyu / Collins, Agnieszka / Mettlen, Marcel / Samelko, Beata / Altintas, Mehmet M / Sudhini, Yashwanth R / Wang, Xuexiang / Bouley, Richard / Brown, Dennis / Pedro, Bradley P / Bane, Susan L / Gupta, Vineet / Brinkkoetter, Paul T / Hagmann, Henning / Reiser, Jochen / Sever, Sanja

    Nature communications

    2022  Volume 13, Issue 1, Page(s) 2422

    Abstract: Chronic kidney diseases and acute kidney injury are mechanistically distinct kidney diseases. While chronic kidney diseases are associated with podocyte injury, acute kidney injury affects renal tubular epithelial cells. Despite these differences, a ... ...

    Abstract Chronic kidney diseases and acute kidney injury are mechanistically distinct kidney diseases. While chronic kidney diseases are associated with podocyte injury, acute kidney injury affects renal tubular epithelial cells. Despite these differences, a cardinal feature of both acute and chronic kidney diseases is dysregulated actin cytoskeleton. We have shown that pharmacological activation of GTPase dynamin ameliorates podocyte injury in murine models of chronic kidney diseases by promoting actin polymerization. Here we establish dynamin's role in modulating stiffness and polarity of renal tubular epithelial cells by crosslinking actin filaments into branched networks. Activation of dynamin's crosslinking capability by a small molecule agonist stabilizes the actomyosin cortex of the apical membrane against injury, which in turn preserves renal function in various murine models of acute kidney injury. Notably, a dynamin agonist simultaneously attenuates podocyte and tubular injury in the genetic murine model of Alport syndrome. Our study provides evidence for the feasibility and highlights the benefits of novel holistic nephron-protective therapies.
    MeSH term(s) Actin Cytoskeleton ; Acute Kidney Injury/prevention & control ; Animals ; Dynamins ; Female ; Humans ; Kidney/physiology ; Male ; Mice ; Podocytes ; Renal Insufficiency, Chronic/drug therapy
    Chemical Substances Dynamins (EC 3.6.5.5)
    Language English
    Publishing date 2022-05-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-022-30101-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Book: Jungproterozoische submarine Primäranreicherung und metamorphogene Weiterentwicklung der Stratiformen W-Sb-Au-Lagerstätten vom "Typ Woxi" in Hunan (Südchina)

    Gu, Xuexiang

    (Archiv für Lagerstättenforschung ; 23.2002)

    2002  

    Author's details Xuexiang Gu
    Series title Archiv für Lagerstättenforschung ; 23.2002
    Language German
    Size 204 S, Ill., graph. Darst., Kt
    Publisher Geologische Bundesanstalt
    Publishing place Wien
    Document type Book
    ISBN 3853160158 ; 9783853160152
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  7. Article: Unraveling the mechanobiology of immune cells

    Zhang, Xuexiang / Kim, Tae-Hyung / Thauland, Timothy J / Li, Hongjun / Majedi, Fatemeh Sadat / Ly, Chau / Gu, Zhen / Butte, Manish J / Rowat, Amy C / Li, Song

    Current opinion in biotechnology. 2020 Dec., v. 66

    2020  

    Abstract: Immune cells can sense and respond to biophysical cues — from dynamic forces to spatial features — during their development, activation, differentiation and expansion. These biophysical signals regulate a variety of immune cell functions such as ... ...

    Abstract Immune cells can sense and respond to biophysical cues — from dynamic forces to spatial features — during their development, activation, differentiation and expansion. These biophysical signals regulate a variety of immune cell functions such as leukocyte extravasation, macrophage polarization, T cell selection and T cell activation. Recent studies have advanced our understanding on immune responses to biophysical cues and the underlying mechanisms of mechanotransduction, which provides rational basis for the design and development of immune-modulatory therapeutics. This review discusses the recent progress in mechanosensing and mechanotransduction of immune cells, particularly monocytes/macrophages and T lymphocytes, and features new biomaterial designs and biomedical devices that translate these findings into biomedical applications.
    Keywords T-lymphocytes ; biocompatible materials ; biophysics ; biotechnology ; macrophages ; mechanotransduction ; monocytes ; therapeutics
    Language English
    Dates of publication 2020-12
    Size p. 236-245.
    Publishing place Elsevier Ltd
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 1052045-4
    ISSN 1879-0429 ; 0958-1669
    ISSN (online) 1879-0429
    ISSN 0958-1669
    DOI 10.1016/j.copbio.2020.09.004
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 3071: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article: Elevated serum aspartate aminotransferase level identifies patients with coronavirus disease 2019 and predicts the length of hospital stay

    Gu, Xuexiang / Li, Xiangyu / An, Xusheng / Yang, Shufeng / Wu, Shangnong / Yang, Xiaozhong / Wang, Honggang

    J Clin Lab Anal

    Abstract: BACKGROUND: Coronavirus disease 2019 (COVID-19) has become a worldwide public health emergency. This study aimed to investigate the clinical significance of liver blood tests in COVID-19 patients. METHODS: The analysis included clinical data of 23 ... ...

    Abstract BACKGROUND: Coronavirus disease 2019 (COVID-19) has become a worldwide public health emergency. This study aimed to investigate the clinical significance of liver blood tests in COVID-19 patients. METHODS: The analysis included clinical data of 23 patients with suspected COVID-19 and 66 patients with confirmed COVID-19 from January 25 to February 20, 2020. The relationship between liver blood test results, liver condition (HBsAb positive, HBcAb positive, and fatty liver disease), and duration of hospital stay among COVID-19 patients was analyzed. RESULTS: The median hospital stay of COVID-19 patients was 6 days. Serum albumin (Alb) level was lower in patients with COVID-19 confirmed on admission than in patients with suspected COVID-19 (40.08 g/L vs 42.50 g/L, P = .016), while the level of aspartate aminotransferase (AST) was higher (23 U/L vs 18 U/L, P = .005). Abnormal results of liver blood tests in patients with COVID-19 included increased levels of alanine transaminase (ALT) (21.2%, 14 patients), AST (15.2%, 10 patients), and gamma-glutamyl transpeptidase (GGT) (22.7%, 15 patients). After 5-10 days of treatment, levels of Alb and AST in COVID-19 patients were significantly decreased (P < .001 and P = .027, respectively). Abnormal levels of Alb and AST in patients with COVID-19 were not associated with the liver condition (all P > .05). In addition, only levels of AST were positively correlated with the duration of hospital stay (r = .334, P = .007). CONCLUSION: Abnormal results of the liver blood test were found in COVID-19 patients. The COVID-19 patients on admission with the higher levels of AST might have longer hospital stays.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #505737
    Database COVID19

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  9. Article ; Online: Unraveling the mechanobiology of immune cells.

    Zhang, Xuexiang / Kim, Tae-Hyung / Thauland, Timothy J / Li, Hongjun / Majedi, Fatemeh Sadat / Ly, Chau / Gu, Zhen / Butte, Manish J / Rowat, Amy C / Li, Song

    Current opinion in biotechnology

    2020  Volume 66, Page(s) 236–245

    Abstract: Immune cells can sense and respond to biophysical cues - from dynamic forces to spatial features - during their development, activation, differentiation and expansion. These biophysical signals regulate a variety of immune cell functions such as ... ...

    Abstract Immune cells can sense and respond to biophysical cues - from dynamic forces to spatial features - during their development, activation, differentiation and expansion. These biophysical signals regulate a variety of immune cell functions such as leukocyte extravasation, macrophage polarization, T cell selection and T cell activation. Recent studies have advanced our understanding on immune responses to biophysical cues and the underlying mechanisms of mechanotransduction, which provides rational basis for the design and development of immune-modulatory therapeutics. This review discusses the recent progress in mechanosensing and mechanotransduction of immune cells, particularly monocytes/macrophages and T lymphocytes, and features new biomaterial designs and biomedical devices that translate these findings into biomedical applications.
    MeSH term(s) Biocompatible Materials ; Biophysics ; Cell Differentiation ; Macrophages ; Mechanotransduction, Cellular
    Chemical Substances Biocompatible Materials
    Keywords covid19
    Language English
    Publishing date 2020-09-30
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1052045-4
    ISSN 1879-0429 ; 0958-1669
    ISSN (online) 1879-0429
    ISSN 0958-1669
    DOI 10.1016/j.copbio.2020.09.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 3071: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  10. Article ; Online: Novel ent-Kaurane Diterpenoid from Rubus corchorifolius L. f. Inhibits Human Colon Cancer Cell Growth via Inducing Cell Cycle Arrest and Apoptosis.

    Chen, Xuexiang / Wu, Xian / Ouyang, Wen / Gu, Min / Gao, Zili / Song, Mingyue / Chen, Yunjiao / Lin, Yanyin / Cao, Yong / Xiao, Hang

    Journal of agricultural and food chemistry

    2017  Volume 65, Issue 8, Page(s) 1566–1573

    Abstract: The tender leaves of Rubus corchorifolius L. f. have been consumed as tea for drinking in China since ancient times. In this study, a novel ent-kaurane diterpenoid was isolated and identified from R. corchorifolius L. f. leaves as ent-kaur-2-one-16β,17- ... ...

    Abstract The tender leaves of Rubus corchorifolius L. f. have been consumed as tea for drinking in China since ancient times. In this study, a novel ent-kaurane diterpenoid was isolated and identified from R. corchorifolius L. f. leaves as ent-kaur-2-one-16β,17-dihydroxy-acetone-ketal (DEK). DEK suppressed the growth of HCT116 human colon cancer cells with an IC
    MeSH term(s) Apoptosis/drug effects ; Cell Cycle Checkpoints/drug effects ; Cell Proliferation/drug effects ; Colon/cytology ; Colon/drug effects ; Colonic Neoplasms/drug therapy ; Colonic Neoplasms/physiopathology ; Diterpenes, Kaurane/pharmacology ; Humans ; Plant Extracts/pharmacology ; Rubus/chemistry
    Chemical Substances Diterpenes, Kaurane ; Plant Extracts
    Language English
    Publishing date 2017-03-01
    Publishing country United States
    Document type Journal Article
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.6b05376
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1172: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top