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  1. Article: Editorial: Plant molecular farming for the production of next-generation vaccines and biologics - prospects and challenges.

    Shanmugaraj, Balamurugan / Hefferon, Kathleen

    Frontiers in plant science

    2024  Volume 15, Page(s) 1381234

    Language English
    Publishing date 2024-02-27
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2024.1381234
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Development of SARS-CoV-2 neutralizing antibody detection assay by using recombinant plant-produced proteins.

    Jirarojwattana, Perawat / Shanmugaraj, Balamurugan / Rattanapisit, Kaewta / Phoolcharoen, Waranyoo

    Biotechnology reports (Amsterdam, Netherlands)

    2023  Volume 38, Page(s) e00796

    Abstract: Detecting immunity against SARS-CoV-2 is vital for evaluating vaccine response and natural infection, but conventional virus neutralization test (cVNT) requires BSL3 and live viruses, and pseudo-virus neutralization test (pVNT) needs specialized ... ...

    Abstract Detecting immunity against SARS-CoV-2 is vital for evaluating vaccine response and natural infection, but conventional virus neutralization test (cVNT) requires BSL3 and live viruses, and pseudo-virus neutralization test (pVNT) needs specialized equipment and trained professionals. The surrogate virus neutralization test (sVNT) was developed to overcome these limitations. This study explored the use of angiotensin converting enzyme 2 (ACE2) produced from
    Language English
    Publishing date 2023-04-06
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2801018-8
    ISSN 2215-017X
    ISSN 2215-017X
    DOI 10.1016/j.btre.2023.e00796
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Molecular Farming Strategy for the Rapid Production of Protein-Based Reagents for Use in Infectious Disease Diagnostics.

    Shanmugaraj, Balamurugan / Jirarojwattana, Perawat / Phoolcharoen, Waranyoo

    Planta medica

    2023  Volume 89, Issue 10, Page(s) 1010–1020

    Abstract: Recombinant proteins are a major breakthrough in biomedical research with a wide range of applications from diagnostics to therapeutics. Strategic construct design, consistent expression platforms, and suitable upstream and downstream techniques are key ... ...

    Abstract Recombinant proteins are a major breakthrough in biomedical research with a wide range of applications from diagnostics to therapeutics. Strategic construct design, consistent expression platforms, and suitable upstream and downstream techniques are key considerations to produce commercially viable recombinant proteins. The recombinant antigenic protein production for use either as a diagnostic reagent or subunit vaccine formulation is usually carried out in prokaryotic or eukaryotic expression platforms. Microbial and mammalian systems dominate the biopharmaceutical industry for such applications. However, there is no universal expression system that can meet all the requirements for different types of proteins. The adoptability of any expression system is likely based on the quality and quantity of the proteins that can be produced from it. The huge demand of recombinant proteins for different applications requires an inexpensive production platform for rapid development. The molecular farming scientific community has been promoting the plant system for nearly 3 decades as a cost-effective alternative to produce high-quality proteins for research, diagnostic, and therapeutic applications. Here, we discuss how plant biotechnology could offer solutions for the rapid and scalable production of protein antigens as low-cost diagnostic reagents for use in functional assays.
    MeSH term(s) Animals ; Molecular Farming ; Plants, Genetically Modified/metabolism ; Biotechnology/methods ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Communicable Diseases/diagnosis ; Mammals/metabolism
    Chemical Substances Recombinant Proteins
    Language English
    Publishing date 2023-04-18
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 123545-x
    ISSN 1439-0221 ; 0032-0943
    ISSN (online) 1439-0221
    ISSN 0032-0943
    DOI 10.1055/a-2076-2034
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uf I Zs.157: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Addressing demand for recombinant biopharmaceuticals in the COVID-19 era

    Balamurugan Shanmugaraj / Waranyoo Phoolcharoen

    Asian Pacific Journal of Tropical Medicine, Vol 14, Iss 2, Pp 49-

    2021  Volume 51

    Keywords Arctic medicine. Tropical medicine ; RC955-962
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher Wolters Kluwer Medknow Publications
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Neutralizing activity of anti-respiratory syncytial virus monoclonal antibody produced in

    Pisuttinusart, Nuttapat / Rattanapisit, Kaewta / Srisaowakarn, Chanya / Thitithanyanont, Arunee / Strasser, Richard / Shanmugaraj, Balamurugan / Phoolcharoen, Waranyoo

    Human vaccines & immunotherapeutics

    2024  Volume 20, Issue 1, Page(s) 2327142

    Abstract: Respiratory syncytial virus (RSV) is a highly contagious virus that affects the lungs and respiratory passages of many vulnerable people. It is a leading cause of lower respiratory tract infections and clinical complications, particularly among infants ... ...

    Abstract Respiratory syncytial virus (RSV) is a highly contagious virus that affects the lungs and respiratory passages of many vulnerable people. It is a leading cause of lower respiratory tract infections and clinical complications, particularly among infants and elderly. It can develop into serious complications such as pneumonia and bronchiolitis. The development of RSV vaccine or immunoprophylaxis remains highly active and a global health priority. Currently, GSK's Arexvy™ vaccine is approved for the prevention of lower respiratory tract disease in older adults (>60 years). Palivizumab and currently nirsevimab are the approved monoclonal antibodies (mAbs) for RSV prevention in high-risk patients. Many studies are ongoing to develop additional therapeutic antibodies for preventing RSV infections among newborns and other susceptible groups. Recently, additional antibodies have been discovered and shown greater potential for development as therapeutic alternatives to palivizumab and nirsevimab. Plant expression platforms have proven successful in producing recombinant proteins, including antibodies, offering a potential cost-effective alternative to mammalian expression platforms. Hence in this study, an attempt was made to use a plant expression platform to produce two anti-RSV fusion (F) mAbs 5C4 and CR9501. The heavy-chain and light-chain sequences of both these antibodies were transiently expressed in
    MeSH term(s) Infant ; Animals ; Humans ; Infant, Newborn ; Aged ; Palivizumab/therapeutic use ; Nicotiana/genetics ; Respiratory Syncytial Virus Infections/prevention & control ; Antibodies, Monoclonal/therapeutic use ; Antibodies, Viral ; Respiratory Syncytial Virus, Human ; Antibodies, Neutralizing ; Viral Fusion Proteins/genetics ; Respiratory Syncytial Virus Vaccines ; Mammals/metabolism
    Chemical Substances Palivizumab (DQ448MW7KS) ; Antibodies, Monoclonal ; Antibodies, Viral ; Antibodies, Neutralizing ; Viral Fusion Proteins ; Respiratory Syncytial Virus Vaccines
    Language English
    Publishing date 2024-03-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2664176-8
    ISSN 2164-554X ; 2164-5515
    ISSN (online) 2164-554X
    ISSN 2164-5515
    DOI 10.1080/21645515.2024.2327142
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6967: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  6. Article ; Online: SARS-CoV-2 omicron variant: Could it be another threat?

    Shanmugaraj, Balamurugan / Malla, Ashwini / Khorattanakulchai, Narach / Phoolcharoen, Waranyoo

    Journal of medical virology

    2022  Volume 94, Issue 4, Page(s) 1284–1288

    MeSH term(s) COVID-19/epidemiology ; COVID-19/prevention & control ; COVID-19/transmission ; COVID-19/virology ; COVID-19 Vaccines/immunology ; Epidemiological Monitoring ; Humans ; International Cooperation ; SARS-CoV-2/genetics ; SARS-CoV-2/pathogenicity ; SARS-CoV-2/physiology ; Spike Glycoprotein, Coronavirus/genetics ; Spike Glycoprotein, Coronavirus/immunology ; Vaccination
    Chemical Substances COVID-19 Vaccines ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
    Language English
    Publishing date 2022-01-14
    Publishing country United States
    Document type Letter ; Research Support, Non-U.S. Gov't
    ZDB-ID 752392-0
    ISSN 1096-9071 ; 0146-6615
    ISSN (online) 1096-9071
    ISSN 0146-6615
    DOI 10.1002/jmv.27532
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1320: Show issues Location:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  7. Article ; Online: SARS-CoV-2 variants

    Balamurugan Shanmugaraj / Narach Khorattanakulchai / Waranyoo Phoolcharoen

    Asian Pacific Journal of Tropical Medicine, Vol 15, Iss 1, Pp 1-

    A continuing threat to global health

    2022  Volume 3

    Keywords Arctic medicine. Tropical medicine ; RC955-962
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher Wolters Kluwer Medknow Publications
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Emergence of monkeypox

    Balamurugan Shanmugaraj / Narach Khorattanakulchai / Waranyoo Phoolcharoen

    Asian Pacific Journal of Tropical Medicine, Vol 15, Iss 5, Pp 193-

    Another concern amidst COVID-19 crisis

    2022  Volume 195

    Keywords Arctic medicine. Tropical medicine ; RC955-962
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher Wolters Kluwer Medknow Publications
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Development of SARS-CoV-2 neutralizing antibody detection assay by using recombinant plant-produced proteins

    Jirarojwattana, Perawat / Shanmugaraj, Balamurugan / Rattanapisit, Kaewta / Phoolcharoen, Waranyoo

    Biotechnology Reports. 2023 June, v. 38 p.e00796-

    2023  

    Abstract: Detecting immunity against SARS-CoV-2 is vital for evaluating vaccine response and natural infection, but conventional virus neutralization test (cVNT) requires BSL3 and live viruses, and pseudo-virus neutralization test (pVNT) needs specialized ... ...

    Abstract Detecting immunity against SARS-CoV-2 is vital for evaluating vaccine response and natural infection, but conventional virus neutralization test (cVNT) requires BSL3 and live viruses, and pseudo-virus neutralization test (pVNT) needs specialized equipment and trained professionals. The surrogate virus neutralization test (sVNT) was developed to overcome these limitations. This study explored the use of angiotensin converting enzyme 2 (ACE2) produced from Nicotiana benthamiana for the development of an affordable neutralizing antibodies detection assay. The results showed that the plant-produced ACE2 can bind to the receptor binding domain (RBD) of the SARS-CoV-2, and was used to develop sVNT with plant-produced RBD protein. The sVNT developed using plant-produced proteins showed high sensitivity and specificity when validated with a group of 30 RBD vaccinated mice sera and the results were correlated with cVNT titer. This preliminary finding suggests that the plants could offer a cost-effective platform for producing diagnostic reagents.
    Keywords Nicotiana benthamiana ; Severe acute respiratory syndrome coronavirus 2 ; antibody detection ; biotechnology ; cost effectiveness ; enzymes ; equipment ; immunity ; neutralization tests ; vaccines ; Surrogate virus neutralization ; Plant-produced ACE2 protein ; SARS-CoV-2 ; Diagnostic reagents ; COVID-19
    Language English
    Dates of publication 2023-06
    Publishing place Elsevier B.V.
    Document type Article ; Online
    Note Use and reproduction
    ZDB-ID 2801018-8
    ISSN 2215-017X
    ISSN 2215-017X
    DOI 10.1016/j.btre.2023.e00796
    Database NAL-Catalogue (AGRICOLA)

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  10. Article: Immunogenicity of a recombinant plant-produced respiratory syncytial virus F subunit vaccine in mice.

    Pisuttinusart, Nuttapat / Shanmugaraj, Balamurugan / Srisaowakarn, Chanya / Ketloy, Chutitorn / Prompetchara, Eakachai / Thitithanyanont, Arunee / Phoolcharoen, Waranyoo

    Biotechnology reports (Amsterdam, Netherlands)

    2023  Volume 41, Page(s) e00826

    Abstract: Respiratory syncytial virus (RSV) is a highly infectious respiratory virus that causes serious illness, particularly in young children, elderly people, and those with immunocompromised individuals. RSV infection is the leading cause of infant ... ...

    Abstract Respiratory syncytial virus (RSV) is a highly infectious respiratory virus that causes serious illness, particularly in young children, elderly people, and those with immunocompromised individuals. RSV infection is the leading cause of infant hospitalization and can lead to serious complications such as pneumonia and bronchiolitis. Currently, there is an RSV vaccine approved exclusively for the elderly population, but no approved vaccine specifically designed for infants or any other age groups. Therefore, it is crucial to continue the development of an RSV vaccine specifically tailored for these populations. In this study, the immunogenicity of the two plant-produced RSV-F Fc fusion proteins (Native construct and structural stabilized construct) were examined to assess them as potential vaccine candidates for RSV. The RSV-F Fc fusion proteins were transiently expressed in
    Language English
    Publishing date 2023-12-23
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2801018-8
    ISSN 2215-017X
    ISSN 2215-017X
    DOI 10.1016/j.btre.2023.e00826
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