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Article ; Online: Impact of particulate deproteinized bovine bone mineral and porous titanium granules on early stability and osseointegration of dental implants in narrow marginal circumferential bone defects.

Verket, A / Lyngstadaas, S P / Tiainen, H / Rønold, H J / Wohlfahrt, J C

International journal of oral and maxillofacial surgery

2018 Volume 47, Issue 8, Page(s) 1086–1094

Abstract: The use of two particulate bone graft substitute materials in experimental narrow marginal peri-implant bone defects was investigated with respect to early bone healing and implant stability. Porous titanium granules, oxidized white porous titanium ... ...

Abstract	The use of two particulate bone graft substitute materials in experimental narrow marginal peri-implant bone defects was investigated with respect to early bone healing and implant stability. Porous titanium granules, oxidized white porous titanium granules (WPTG), and demineralized bovine bone mineral (DBBM) were characterized in vitro, after which the two latter materials were tested in experimental peri-implant bone defects in six minipigs, with empty defects as control. After mandibular premolar extraction, the top 5mm of the alveoli were widened to 6mm in diameter, followed by the placement of six implants, three on each side, in each pig. Six weeks of healing was allowed. The WPTG showed better mechanical properties. No significant differences in resonance frequency analysis were found directly after compacting or healing, and similar quantities of defect bone formation were observed on micro-computed tomography for all groups. Histomorphometric analysis demonstrated a more coronal bone-to-implant contact in the DBBM group, which also displayed more defect bone fill as compared to the WPTG group. The better mechanical properties observed for WPTG appear of negligible relevance for the early stability and osseointegration of implants.
MeSH term(s)	Animals ; Bone Substitutes/pharmacology ; Cattle ; Dental Implantation, Endosseous/methods ; Dental Implants ; Female ; Microscopy, Electron, Scanning ; Osseointegration/drug effects ; Porosity ; Random Allocation ; Swine ; Swine, Miniature ; Titanium/pharmacology ; Wound Healing/drug effects ; X-Ray Microtomography
Chemical Substances	Bone Substitutes ; Dental Implants ; Titanium (D1JT611TNE)
Language	English
Publishing date	2018-03-20
Publishing country	Denmark
Document type	Clinical Trial, Veterinary ; Journal Article
ZDB-ID	353721-3
ISSN	1399-0020 ; 0901-5027
ISSN (online)	1399-0020
ISSN	0901-5027
DOI	10.1016/j.ijom.2018.02.007
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Zs.A 931: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 401: Show issues

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Article: Phosphorylation Modulates Ameloblastin Self-assembly and Ca

Stakkestad, Øystein / Lyngstadaas, Ståle P / Thiede, Bernd / Vondrasek, Jiri / Skålhegg, Bjørn S / Reseland, Janne E

Frontiers in physiology

2017 Volume 8, Page(s) 531

Abstract: Ameloblastin (AMBN), an important component of the self-assembled enamel extra cellular matrix, contains ... ...

Abstract	Ameloblastin (AMBN), an important component of the self-assembled enamel extra cellular matrix, contains several
Language	English
Publishing date	2017-07-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2564217-0
ISSN	1664-042X
ISSN	1664-042X
DOI	10.3389/fphys.2017.00531
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Article: Synthetic hammerhead ribozymes as tools in gene expression.

Lyngstadaas, S P

Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists

2001 Volume 12, Issue 6, Page(s) 469–478

Abstract: ... for the gene(s) involved. One promising strategy to overcome this problem is through the use of ribozymes ...

Abstract	The assessment of genetic controls for sequential developmental processes such as tooth formation and biomineralization is often difficult in transgenic "knockout" models, where phenotypes reflect only the permanent eradication of a gene, and reveal little about the dynamic range of expression for the gene(s) involved. One promising strategy to overcome this problem is through the use of ribozymes, a class of metalloenzymes made entirely of ribonucleic acid (RNA), that are capable of cleaving other RNA molecules in a catalytic fashion. Their activity can be targeted against specific mRNAs by selection of unique sequences flanking a conserved catalytic motif. In synthetic ribozymes, specificity, stability, and cell permeability can be dramatically improved by the incorporation of chemically modified ribonucleotides. This review focuses on the design and application of hammerhead ribozymes, the best-known and most widely used class of RNA-based enzymes. So far, except for a few conserved structures at the catalytic core, no one particular model or superior ribozyme design has been identified. It may well be that each cell, tissue, and organism has different requirements for the uptake, activity, and stability of hammerhead ribozymes. However, designed ribozymes can be highly effective agents for timed and localized elimination of gene products. As the 3D structures of active hammerhead molecules are revealed, more effective ribozymes will be developed. Today, developments in ribozyme-mediated sequence-specific blocking of gene expression hold great promise for active RNA enzymes as tools in biomolecular research and for eliminating unwanted gene expression in human diseases.
MeSH term(s)	Animals ; Drug Design ; Enzyme Stability ; Gene Expression/drug effects ; Gene Silencing/drug effects ; Humans ; Molecular Structure ; Protein Conformation ; Protein Engineering ; RNA, Catalytic/chemical synthesis ; RNA, Catalytic/chemistry ; RNA, Catalytic/pharmacology ; Substrate Specificity ; Transfection/methods
Chemical Substances	RNA, Catalytic ; hammerhead ribozyme
Language	English
Publishing date	2001-12-31
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	1130962-3
ISSN	1045-4411
ISSN	1045-4411
DOI	10.1177/10454411010120060201
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Zs.A 3504: Show issues

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Article ; Online: A single amino acid deletion in the ER Ca

Science signaling

2023 Volume 16, Issue 771, Page(s) eadd0509

Abstract: Stormorken syndrome is a multiorgan hereditary disease caused by dysfunction of the endoplasmic reticulum (ER) ... ...

Abstract	Stormorken syndrome is a multiorgan hereditary disease caused by dysfunction of the endoplasmic reticulum (ER) Ca
MeSH term(s)	Mice ; Animals ; Membrane Proteins/metabolism ; Calcium Channels/metabolism ; Amino Acids/metabolism ; Mutation ; Endoplasmic Reticulum/metabolism ; Stromal Interaction Molecule 1/genetics ; Calcium Release Activated Calcium Channels/genetics ; ORAI1 Protein/metabolism ; Calcium/metabolism
Chemical Substances	Membrane Proteins ; Calcium Channels ; Amino Acids ; Stromal Interaction Molecule 1 ; Calcium Release Activated Calcium Channels ; ORAI1 Protein ; Calcium (SY7Q814VUP) ; Stim1 protein, mouse
Language	English
Publishing date	2023-02-07
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2417226-1
ISSN	1937-9145 ; 1945-0877
ISSN (online)	1937-9145
ISSN	1945-0877
DOI	10.1126/scisignal.add0509
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Article ; Online: The influence of surface nanoroughness, texture and chemistry of TiZr implant abutment on oral biofilm accumulation.

Xing, Rui / Lyngstadaas, Ståle P / Ellingsen, Jan Eirik / Taxt-Lamolle, Sébastien / Haugen, Håvard J

Clinical oral implants research

2015 Volume 26, Issue 6, Page(s) 649–656

Abstract: ... with hydrogen by cathodic polarization and/or acid etching with HCl/H(2)SO(4). Nanoroughness (S(a)) positively ...

Abstract	Objectives: The aim of the study was to examine surface nanoroughness, texture and chemistry of dental implant abutment and to investigate how these parameters influence oral biofilm formation in healthy subjects. Materials and methods: Eight different nanorough TiZr surfaces were produced by polishing, machining, cathodic polarization and acid etching. Surface topography was examined using field emission scanning electron microscope and a blue light laser profilometer. Surface chemistry was analyzed by secondary ion mass spectrometry and X-ray photoelectron spectroscopy. Surface hydrophilicity was tested by measuring contact angle on the surfaces. A human in vivo study using a splint model was employed to evaluate oral biofilm accumulation on these surfaces. Results: Different surface textures (flat, grooved and irregular) were created with nanoroughness from 29 to 214 nm. Some test surfaces were incorporated with hydrogen by cathodic polarization and/or acid etching with HCl/H(2)SO(4). Nanoroughness (S(a)) positively correlated with microbial adhesion. Biofilm accumulation was less pronounced on flat and grooved than on irregular surfaces. No significant association between hydrogen content or hydrophilicity of the surface and biofilm accumulation was observed. Conclusions: Nanoroughness (< 214 nm) and surface texture influence oral biofilm accumulation independent of surface chemistry and hydrophilicity. Surface hydrogen, which has previously been shown to promote fibroblast growth, does not affect biofilm formation.
MeSH term(s)	Acid Etching, Dental/methods ; Adult ; Biofilms/growth & development ; Dental Abutments/microbiology ; Dental Implants/microbiology ; Female ; Humans ; Hydrophobic and Hydrophilic Interactions ; Male ; Photoelectron Spectroscopy ; Surface Properties ; Titanium/chemistry ; Zirconium/chemistry
Chemical Substances	Dental Implants ; Zirconium (C6V6S92N3C) ; Titanium (D1JT611TNE)
Language	English
Publishing date	2015-06
Publishing country	Denmark
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1067626-0
ISSN	1600-0501 ; 0905-7161
ISSN (online)	1600-0501
ISSN	0905-7161
DOI	10.1111/clr.12354
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Zs.A 3171: Show issues

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Article ; Online: Effect of alginate hydrogel containing polyproline-rich peptides on osteoblast differentiation.

Rubert, M / Monjo, M / Lyngstadaas, S P / Ramis, J M

Biomedical materials (Bristol, England)

2012 Volume 7, Issue 5, Page(s) 55003

Abstract: Polyproline-rich synthetic peptides have previously been shown to induce bone formation and mineralization in vitro and to decrease bone resorption in vivo. Alginate hydrogel formulations containing these synthetic peptides (P2, P5, P6) or Emdogain® (EMD) ...

Abstract	Polyproline-rich synthetic peptides have previously been shown to induce bone formation and mineralization in vitro and to decrease bone resorption in vivo. Alginate hydrogel formulations containing these synthetic peptides (P2, P5, P6) or Emdogain® (EMD) were tested for surface coating of bone implants. In an aqueous environment, the alginate hydrogels disclosed a highly compact structure suitable for cell adhesion and proliferation. Lack of cytotoxicity of the alginate-gel coating containing peptides was tested in MC3T3-E1 cell cultures. In the present study, relative mRNA expression levels of integrin alpha 8 were induced by P5 compared to untreated alginate gel, and osteopontin mRNA levels were increased after 21 days of culture by treatment with synthetic peptides or EMD compared to control. Further, in agreement with previous results when the synthetic peptides were administered in the culture media, osteocalcin mRNA was significantly upregulated after long-term treatment with the formulated synthetic peptides compared to untreated and EMD alginate gel. These results indicate that the alginate gel is a suitable carrier for the delivery of synthetic peptides, and that the formulation is promising as biodegradable and biocompatible coating for bone implants.
MeSH term(s)	3T3 Cells ; Alginates ; Amino Acid Sequence ; Animals ; Base Sequence ; Bone Substitutes/chemistry ; Cell Adhesion ; Cell Differentiation/drug effects ; Cell Proliferation ; Coated Materials, Biocompatible/chemistry ; Dental Enamel Proteins/chemistry ; Dental Enamel Proteins/pharmacology ; Glucuronic Acid ; Hexuronic Acids ; Hydrogels ; Integrin alpha Chains/genetics ; Materials Testing ; Mice ; Microscopy, Electron, Scanning ; Molecular Sequence Data ; Osteoblasts/cytology ; Osteoblasts/drug effects ; Osteoblasts/metabolism ; Osteopontin/genetics ; Peptides/chemistry ; Peptides/pharmacology ; RNA, Messenger/genetics ; RNA, Messenger/metabolism
Chemical Substances	Alginates ; Bone Substitutes ; Coated Materials, Biocompatible ; Dental Enamel Proteins ; Hexuronic Acids ; Hydrogels ; Integrin alpha Chains ; Peptides ; RNA, Messenger ; Spp1 protein, mouse ; enamel matrix proteins ; integrin alpha8 ; Osteopontin (106441-73-0) ; polyproline (25191-13-3) ; Glucuronic Acid (8A5D83Q4RW) ; alginic acid (8C3Z4148WZ)
Language	English
Publishing date	2012-10
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2265222-X
ISSN	1748-605X ; 1748-6041
ISSN (online)	1748-605X
ISSN	1748-6041
DOI	10.1088/1748-6041/7/5/055003
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Zs.A 6626: Show issues

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Article ; Online: Osseointegration of dental implants in extraction sockets preserved with porous titanium granules - an experimental study.

Verket, Anders / Lyngstadaas, Ståle P / Rønold, Hans J / Wohlfahrt, Johan C

Clinical oral implants research

2014 Volume 25, Issue 2, Page(s) e100–8

Abstract: ... female minipigs (Gøttingen minipig; Ellegaard A/S, Dalmose, Denmark) had the mandibular teeth P2, P3 and ... group (P = 0.03) and 57.1% for the sham group. Histomorphometry demonstrated an average bone-to-implant ... contact of 68.2% for the PTG group compared to 36.6% for the WPTG group and 60.9% for the sham group (n.s ...

Abstract	Objectives: This study investigated osseointegration of dental implants inserted in healed extraction sockets preserved with porous titanium granules (PTG). Material and methods: Three adult female minipigs (Gøttingen minipig; Ellegaard A/S, Dalmose, Denmark) had the mandibular teeth P2, P3 and P4 extracted. The extraction sockets were preserved with metallic PTG (Tigran PTG; Tigran Technologies AB, Malmö, Sweden) n = 12, heat oxidized white porous titanium granules (WPTG) (Tigran PTG White) n = 12 or left empty (sham) n = 6. All sites were covered with collagen membranes (Bio-Gide; Geistlich Pharma, Wolhausen, Switzerland) and allowed 11 weeks of healing before implants (Straumann Bone Level; Straumann, Basel, Switzerland) were inserted. The temperature was measured during preparation of the osteotomies. Resonance frequency analysis (RFA, Osstell; Osstell AB, Gothenburg, Sweden) was performed at implant insertion and at termination. After 6 weeks of submerged implant healing, the pigs were euthanized and jaw segments were excised for microCT and histological analyses. Results: In the temperature and RFA analyses no significant differences were recorded between the test groups. The microCT analysis demonstrated an average bone volume of 61.7% for the PTG group compared to 50.3% for the WPTG group (P = 0.03) and 57.1% for the sham group. Histomorphometry demonstrated an average bone-to-implant contact of 68.2% for the PTG group compared to 36.6% for the WPTG group and 60.9% for the sham group (n.s). Eight out of ten implants demonstrated apical osseous defects in the WPTG group, but similar defects were observed in all groups. Conclusions: PTG preserved extraction sockets demonstrate a similar outcome as the sham control group for all analyses suggesting that this material potentially can be used for extraction socket preservation prior to implant installment. Apical osseous defects were however observed in all groups including the sham group, and a single cause could not be determined.
MeSH term(s)	Animals ; Bone Substitutes/pharmacology ; Collagen/pharmacology ; Dental Implantation, Endosseous/methods ; Dental Implants ; Female ; Implants, Experimental ; Mandible/surgery ; Osseointegration/physiology ; Porosity ; Swine ; Swine, Miniature ; Titanium/pharmacology ; Tooth Extraction ; Tooth Socket/surgery ; Wound Healing/physiology ; X-Ray Microtomography
Chemical Substances	Bio-Gide ; Bone Substitutes ; Dental Implants ; Collagen (9007-34-5) ; Titanium (D1JT611TNE)
Language	English
Publishing date	2014-02
Publishing country	Denmark
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1067626-0
ISSN	1600-0501 ; 0905-7161
ISSN (online)	1600-0501
ISSN	0905-7161
DOI	10.1111/clr.12070
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Article ; Online: Signaling Pathways Used by the Specialized Pro-Resolving Mediator Maresin 2 Regulate Goblet Cell Function

Markus V. Olsen / Anne V. Lyngstadaas / Jeffrey A. Bair / Robin R. Hodges / Tor P. Utheim / Charles N. Serhan / Darlene A. Dartt

International Journal of Molecular Sciences, Vol 23, Iss 6233, p

Comparison with Maresin 1

2022 Volume 6233

Abstract: ... film homeostasis and resolve conjunctival inflammation. We investigated MaR2′s signaling pathways ...

Abstract	Specialized pro-resolving mediators (SPMs), including Maresins (MaR)-1 and 2, contribute to tear film homeostasis and resolve conjunctival inflammation. We investigated MaR2′s signaling pathways in goblet cells (GC) from rat conjunctiva. Agonist-induced [Ca 2+ ] i and high-molecular weight glycoconjugate secretion were measured. MaR2 increased [Ca 2+ ] i and stimulated secretion. MaR2 and MaR1 stimulate conjunctival goblet cell function, especially secretion, by activating different but overlapping GPCR and signaling pathways, and furthermore counter-regulate histamine stimulated increase in [Ca 2+ ] i . Thus, MaR2 and MaR1 play a role in maintaining the ocular surface and tear film homeostasis in health and disease. As MaR2 and MaR1 modulate conjunctival goblet cell function, they each may have potential as novel, but differing, options for the treatment of ocular surface inflammatory diseases including allergic conjunctivitis and dry eye disease. We conclude that in conjunctival GC MaR2 and MaR1, both increase the [Ca 2+ ] i and stimulate secretion to maintain homeostasis by using one set of different, but overlapping, signaling pathways to increase [Ca 2+ ] i and another set to stimulate secretion. MaR2 also resolves ocular allergy.
Keywords	mucin secretion ; intracellular Ca 2+ ; inflammation ; epithelial cell ; tear film ; eye ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	333
Language	English
Publishing date	2022-06-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
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Article ; Online: STIM1 R304W in mice causes subgingival hair growth and an increased fraction of trabecular bone.

Gamage, Thilini H / Lengle, Emma / Gunnes, Gjermund / Pullisaar, Helen / Holmgren, Asbjørn / Reseland, Janne E / Merckoll, Else / Corti, Stefania / Mizobuchi, Masahiro / Morales, Raul J / Tsiokas, Leonidas / Tjønnfjord, Geir E / Lacruz, Rodrigo S / Lyngstadaas, Staale P / Misceo, Doriana / Frengen, Eirik

Cell calcium

2019 Volume 85, Page(s) 102110

Abstract: Calcium signaling plays a central role in bone development and homeostasis. Store operated calcium entry (SOCE) is an important calcium influx pathway mediated by calcium release activated calcium (CRAC) channels in the plasma membrane. Stromal ... ...

Abstract	Calcium signaling plays a central role in bone development and homeostasis. Store operated calcium entry (SOCE) is an important calcium influx pathway mediated by calcium release activated calcium (CRAC) channels in the plasma membrane. Stromal interaction molecule 1 (STIM1) is an endoplasmic reticulum calcium sensing protein important for SOCE. We generated a mouse model expressing the STIM1 R304W mutation, causing Stormorken syndrome in humans. Stim1
MeSH term(s)	Animals ; Bone and Bones/abnormalities ; Bone and Bones/pathology ; Cancellous Bone/pathology ; Cortical Bone/diagnostic imaging ; Cortical Bone/pathology ; Gingiva/growth & development ; Hair/growth & development ; Hair/ultrastructure ; Homozygote ; Incisor/pathology ; Kyphosis/genetics ; Kyphosis/pathology ; Megakaryocytes/metabolism ; Megakaryocytes/pathology ; Mice ; Mutation ; Osteoblasts/metabolism ; Osteoblasts/pathology ; Osteocytes/metabolism ; Osteocytes/pathology ; Ribs/diagnostic imaging ; Ribs/pathology ; Splenomegaly/pathology ; Stromal Interaction Molecule 1/metabolism ; Thorax/pathology ; X-Ray Microtomography
Chemical Substances	Stromal Interaction Molecule 1
Language	English
Publishing date	2019-11-13
Publishing country	Netherlands
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	757687-0
ISSN	1532-1991 ; 0143-4160
ISSN (online)	1532-1991
ISSN	0143-4160
DOI	10.1016/j.ceca.2019.102110
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Zs.A 1596: Show issues

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Article ; Online: Amelogenins modulate cytokine expression in LPS-challenged cultured human macrophages.

Almqvist, Sofia / Werthén, Maria / Lyngstadaas, S Petter / Gretzer, Christina / Thomsen, Peter

Cytokine

2012 Volume 58, Issue 2, Page(s) 274–279

Abstract: ... of alternative macrophage activation-associated CC chemokine-1 (AMAC-1, also known as CCL18; p<0.001), a well ...

Abstract	Amelogenins are enamel matrix proteins with a proven ability to restore tissues in patients with advanced periodontitis and chronic skin wounds. To explore the mechanisms of action of amelogenins in wound inflammation, the in vitro effect on the expression of selected cell mediators involved in inflammation and tissue repair from human monocyte-derived macrophages was studied. Macrophages were treated with amelogenins in serum-enriched medium with simultaneous lipopolysaccharide (LPS) stimulation, for 6, 24 and 72 h, and the conditioned culture medium was analysed for 28 different cytokines. Amelogenin treatment directed the LPS-induced release of both pro- and anti-inflammatory cytokines towards an alternatively activated macrophage phenotype. This change in activation was also demonstrated by the amelogenin-induced secretion of alternative macrophage activation-associated CC chemokine-1 (AMAC-1, also known as CCL18; p<0.001), a well-documented marker of alternative activation. Amelogenins were also shown significantly to increase the macrophage expression of vascular endothelial growth factor and, to a lesser but significant extent, insulin-like growth factor-1 after 24h of culture. The results of the present in vitro study show that monocyte-derived macrophages stimulated by inflammatory agonist LPS respond to the treatment with amelogenins by reducing the pro-inflammatory activity and increasing the expression of tissue repair mediators.
MeSH term(s)	Amelogenin/physiology ; Cells, Cultured ; Culture Media, Conditioned ; Cytokines/metabolism ; Humans ; Lipopolysaccharides/pharmacology ; Macrophages/drug effects ; Macrophages/metabolism
Chemical Substances	Amelogenin ; Culture Media, Conditioned ; Cytokines ; Lipopolysaccharides
Language	English
Publishing date	2012-05
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1018055-2
ISSN	1096-0023 ; 1043-4666
ISSN (online)	1096-0023
ISSN	1043-4666
DOI	10.1016/j.cyto.2012.02.001
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Zs.A 2840: Show issues

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