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Article: Antimicrobial Coating Efficacy for Prevention of

Mettler, Madelyn K / Parker, Ceth W / Venkateswaran, Kasthuri / Peyton, Brent M

2022 Volume 13, Page(s) 874236

Abstract: Biofilms can lead to biofouling, microbially induced corrosion, physical impediment and eventual loss in function of water systems, and other engineered systems. The remoteness and closed environment of the International Space Station (ISS) make it ... ...

Abstract	Biofilms can lead to biofouling, microbially induced corrosion, physical impediment and eventual loss in function of water systems, and other engineered systems. The remoteness and closed environment of the International Space Station (ISS) make it vulnerable to unchecked biofilm growth; thus, biofilm mitigation strategies are crucial for current ISS operation and future long duration and deep-space crewed missions. In this study, a space flown bacterial strain of
Language	English
Publishing date	2022-04-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2022.874236
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Comparative genomic analysis of

Simpson, Anna C / Eedara, V V Ramprasad / Singh, Nitin K / Damle, Namita / Parker, Ceth W / Karouia, Fathi / Mason, Christopher E / Venkateswaran, Kasthuri

Frontiers in microbiology

2023 Volume 14, Page(s) 1166013

Abstract: A single strain from the ... ...

Abstract	A single strain from the family
Language	English
Publishing date	2023-06-15
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2023.1166013
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Novel spore-forming species exhibiting intrinsic resistance to third- and fourth-generation cephalosporins and description of

Miliotis, Georgios / Sengupta, Pratyay / Hameed, Asif / Chuvochina, Maria / McDonagh, Francesca / Simpson, Anna C / Parker, Ceth W / Singh, Nitin K / Rekha, Punchappady D / Morris, Dearbháile / Raman, Karthik / Kyrpides, Nikos C / Hugenholtz, Philip / Venkateswaran, Kasthuri

mBio

2024 Volume 15, Issue 4, Page(s) e0018124

Abstract: A comprehensive microbial surveillance was conducted at NASA's Mars 2020 spacecraft assembly facility (SAF), where whole-genome sequencing (WGS) of 110 bacterial strains was performed. One isolate, designated 179-BFC-A-HS: Importance: The significance ...

Abstract	A comprehensive microbial surveillance was conducted at NASA's Mars 2020 spacecraft assembly facility (SAF), where whole-genome sequencing (WGS) of 110 bacterial strains was performed. One isolate, designated 179-BFC-A-HS Importance: The significance of this research extends to understanding microbial survival and adaptation in oligotrophic environments, such as those found in SAF. Whole-genome sequencing of several strains isolated from Mars 2020 mission assembly cleanroom facilities, including the discovery of the novel species
MeSH term(s)	Humans ; Fatty Acids/analysis ; Ceftazidime ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Base Composition ; Nucleic Acid Hybridization ; Spores/chemistry ; Nucleotides ; DNA ; DNA, Bacterial/genetics ; DNA, Bacterial/chemistry ; Sequence Analysis, DNA ; Bacterial Typing Techniques
Chemical Substances	Fatty Acids ; Ceftazidime (9M416Z9QNR) ; RNA, Ribosomal, 16S ; Nucleotides ; DNA (9007-49-2) ; DNA, Bacterial
Language	English
Publishing date	2024-03-13
Publishing country	United States
Document type	Journal Article
ZDB-ID	2557172-2
ISSN	2150-7511 ; 2161-2129
ISSN (online)	2150-7511
ISSN	2161-2129
DOI	10.1128/mbio.00181-24
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Article ; Online: Metabolic modeling of the International Space Station microbiome reveals key microbial interactions.

Kumar, Rachita K / Singh, Nitin Kumar / Balakrishnan, Sanjaay / Parker, Ceth W / Raman, Karthik / Venkateswaran, Kasthuri

Microbiome

2022 Volume 10, Issue 1, Page(s) 102

Abstract: Background: Recent studies have provided insights into the persistence and succession of microbes aboard the International Space Station (ISS), notably the dominance of Klebsiella pneumoniae. However, the interactions between the various microbes aboard ...

Abstract	Background: Recent studies have provided insights into the persistence and succession of microbes aboard the International Space Station (ISS), notably the dominance of Klebsiella pneumoniae. However, the interactions between the various microbes aboard the ISS and how they shape the microbiome remain to be clearly understood. In this study, we apply a computational approach to predict possible metabolic interactions in the ISS microbiome and shed further light on its organization. Results: Through a combination of a systems-based graph-theoretical approach, and a constraint-based community metabolic modeling approach, we demonstrated several key interactions in the ISS microbiome. These complementary approaches provided insights into the metabolic interactions and dependencies present amongst various microbes in a community, highlighting key interactions and keystone species. Our results showed that the presence of K. pneumoniae is beneficial to many other microorganisms it coexists with, notably those from the Pantoea genus. Species belonging to the Enterobacteriaceae family were often found to be the most beneficial for the survival of other microorganisms in the ISS microbiome. However, K. pneumoniae was found to exhibit parasitic and amensalistic interactions with Aspergillus and Penicillium species, respectively. To prove this metabolic prediction, K. pneumoniae and Aspergillus fumigatus were co-cultured under normal and simulated microgravity, where K. pneumoniae cells showed parasitic characteristics to the fungus. The electron micrography revealed that the presence of K. pneumoniae compromised the morphology of fungal conidia and degenerated its biofilm-forming structures. Conclusion: Our study underscores the importance of K. pneumoniae in the ISS, and its potential positive and negative interactions with other microbes, including potential pathogens. This integrated modeling approach, combined with experiments, demonstrates the potential for understanding the organization of other such microbiomes, unravelling key organisms and their interdependencies. Video Abstract.
MeSH term(s)	Biofilms ; Coculture Techniques ; Enterobacteriaceae ; Microbial Interactions ; Microbiota
Language	English
Publishing date	2022-07-06
Publishing country	England
Document type	Journal Article ; Video-Audio Media ; Research Support, Non-U.S. Gov't
ZDB-ID	2697425-3
ISSN	2049-2618 ; 2049-2618
ISSN (online)	2049-2618
ISSN	2049-2618
DOI	10.1186/s40168-022-01279-y
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Article ; Online: A Novel and Sensitive Method for the Analysis of Fatty Acid Biosignatures by Capillary Electrophoresis-Mass Spectrometry.

Kok, Miranda G M / Mora, Maria F / Noell, Aaron C / Parker, Ceth W / Willis, Peter A

Analytical chemistry

2022 Volume 94, Issue 37, Page(s) 12807–12814

Abstract: Fatty acids are a well-established class of compounds targeted as biosignatures for future missions to look for evidence of life on ocean worlds such as Europa and Enceladus. In order to establish their abiotic or biotic origin, we need to separate and ... ...

Abstract	Fatty acids are a well-established class of compounds targeted as biosignatures for future missions to look for evidence of life on ocean worlds such as Europa and Enceladus. In order to establish their abiotic or biotic origin, we need to separate and quantify fatty acids to determine their relative abundances within a sample. In this study, we demonstrate the high potential of capillary electrophoresis coupled to mass spectrometry (CE-MS) for the efficient separation and sensitive detection of a wide variety of fatty acids. Three derivatization strategies were evaluated to allow the detection of fatty acids by positive ionization mode MS. Furthermore, CE-MS conditions were optimized to provide maximum separation efficiencies and detection sensitivities for the analysis of saturated and unsaturated fatty acids with even- and odd-numbered carbon chain lengths. Optimum separation and detection were obtained using a background electrolyte of 2 M acetic acid in 45% acetonitrile, after derivatization of the fatty acids with 2-picolylamine or
MeSH term(s)	Acetates ; Acetonitriles ; Carbon ; Electrolytes ; Electrophoresis, Capillary/methods ; Fatty Acids ; Mass Spectrometry/methods
Chemical Substances	Acetates ; Acetonitriles ; Electrolytes ; Fatty Acids ; Carbon (7440-44-0)
Language	English
Publishing date	2022-09-06
Publishing country	United States
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.2c02716
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Article: The Isolation and Characterization of Rare Mycobiome Associated With Spacecraft Assembly Cleanrooms.

Blachowicz, Adriana / Mhatre, Snehit / Singh, Nitin Kumar / Wood, Jason M / Parker, Ceth W / Ly, Cynthia / Butler, Daniel / Mason, Christopher E / Venkateswaran, Kasthuri

Frontiers in microbiology

2022 Volume 13, Page(s) 777133

Abstract: Ensuring biological cleanliness while assembling and launching spacecraft is critical for robotic exploration of the solar system. To date, when preventing forward contamination of other celestial bodies, NASA Planetary Protection policies have focused ... ...

Abstract	Ensuring biological cleanliness while assembling and launching spacecraft is critical for robotic exploration of the solar system. To date, when preventing forward contamination of other celestial bodies, NASA Planetary Protection policies have focused on endospore-forming bacteria while fungi were neglected. In this study, for the first time the mycobiome of two spacecraft assembly facilities at Jet Propulsion Laboratory (JPL) and Kennedy Space Center (KSC) was assessed using both cultivation and sequencing techniques. To facilitate enumeration of viable fungal populations and downstream molecular analyses, collected samples were first treated with chloramphenicol for 24 h and then with propidium monoazide (PMA). Among cultivable fungi, 28 distinct species were observed, 16 at JPL and 16 at KSC facilities, while 13 isolates were potentially novel species. Only four isolated species
Language	English
Publishing date	2022-04-26
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2022.777133
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Article ; Online: A Novel and Sensitive Method for the Analysis of Fatty Acid Biosignatures by Capillary Electrophoresis-Mass Spectrometry

Kok, Miranda G. M. / Mora, Maria F. / Noell, Aaron C. / Parker, Ceth W. / Willis, Peter A.

Analytical Chemistry. 2022 Sept. 06, v. 94, no. 37 p.12807-12814

2022

Abstract	Fatty acids are a well-established class of compounds targeted as biosignatures for future missions to look for evidence of life on ocean worlds such as Europa and Enceladus. In order to establish their abiotic or biotic origin, we need to separate and quantify fatty acids to determine their relative abundances within a sample. In this study, we demonstrate the high potential of capillary electrophoresis coupled to mass spectrometry (CE-MS) for the efficient separation and sensitive detection of a wide variety of fatty acids. Three derivatization strategies were evaluated to allow the detection of fatty acids by positive ionization mode MS. Furthermore, CE-MS conditions were optimized to provide maximum separation efficiencies and detection sensitivities for the analysis of saturated and unsaturated fatty acids with even- and odd-numbered carbon chain lengths. Optimum separation and detection were obtained using a background electrolyte of 2 M acetic acid in 45% acetonitrile, after derivatization of the fatty acids with 2-picolylamine or N,N-diethylethylenediamine. The limits of detection for the derivatized fatty acids using the optimized method ranged from 25 to 250 nM. The optimized method was also used for the analysis of fatty acids in cell cultures and natural samples. Two distinctive biosignatures were obtained for the microorganisms Halobacillus halophilus and Pseudoalteromonas haloplanktis. In addition, multiple fatty acids were detected in a natural sample from Mono Lake, California.
Keywords	Halobacillus halophilus ; Pseudoalteromonas haloplanktis ; acetic acid ; acetonitrile ; analytical chemistry ; capillary electrophoresis ; carbon ; derivatization ; electrolytes ; fatty acids ; ionization ; lakes ; mass spectrometry ; California
Language	English
Dates of publication	2022-0906
Size	p. 12807-12814.
Publishing place	American Chemical Society
Document type	Article ; Online
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.2c02716
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Enhanced terrestrial Fe(II) mobilization identified through a novel mechanism of microbially driven cave formation in Fe(III)-rich rocks.

Parker, Ceth W / Senko, John M / Auler, Augusto S / Sasowsky, Ira D / Schulz, Frederik / Woyke, Tanja / Barton, Hazel A

Scientific reports

2022 Volume 12, Issue 1, Page(s) 17062

Abstract: Most cave formation requires mass separation from a host rock in a process that operates outward from permeable pathways to create the cave void. Given the poor solubility of Fe(III) phases, such processes are insufficient to account for the significant ... ...

Abstract	Most cave formation requires mass separation from a host rock in a process that operates outward from permeable pathways to create the cave void. Given the poor solubility of Fe(III) phases, such processes are insufficient to account for the significant iron formation caves (IFCs) seen in Brazilian banded iron formations (BIF) and associated rock. In this study we demonstrate that microbially-mediated reductive Fe(III) dissolution is solubilizing the poorly soluble Fe(III) phases to soluble Fe(II) in the anoxic zone behind cave walls. The resultant Fe(III)-depleted material (termed sub muros) is unable to maintain the structural integrity of the walls and repeated rounds of wall collapse lead to formation of the cave void in an active, measurable process. This mechanism may move significant quantities of Fe(II) into ground water and may help to explain the mechanism of BIF dissolution and REE enrichment in the generation of canga. The role of Fe(III) reducing microorganism and mass separation behind the walls (outward-in, rather than inward-out) is not only a novel mechanism of speleogenesis, but it also may identify a previously overlooked source of continental Fe that may have contributed to Archaean BIF formation.
MeSH term(s)	Caves ; Ferric Compounds/chemistry ; Ferrous Compounds/metabolism ; Iron/metabolism ; Oxidation-Reduction
Chemical Substances	Ferric Compounds ; Ferrous Compounds ; Iron (E1UOL152H7)
Language	English
Publishing date	2022-10-12
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-21365-3
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Article ; Online: Comparative genomic analysis of Cohnella hashimotonis sp. nov. isolated from the International Space Station

Anna C. Simpson / V. V. Ramprasad Eedara / Nitin K. Singh / Namita Damle / Ceth W. Parker / Fathi Karouia / Christopher E. Mason / Kasthuri Venkateswaran

Frontiers in Microbiology, Vol

2023 Volume 14

Abstract: A single strain from the family Paenibacillaceae was isolated from the wall behind the Waste Hygiene Compartment aboard the International Space Station (ISS) in April 2018, as part of the Microbial Tracking mission series. This strain was identified as a ...

Abstract	A single strain from the family Paenibacillaceae was isolated from the wall behind the Waste Hygiene Compartment aboard the International Space Station (ISS) in April 2018, as part of the Microbial Tracking mission series. This strain was identified as a gram-positive, rod-shaped, oxidase-positive, catalase-negative motile bacterium in the genus Cohnella, designated as F6_2S_P_1T. The 16S sequence of the F6_2S_P_1T strain places it in a clade with C. rhizosphaerae and C. ginsengisoli, which were originally isolated from plant tissue or rhizosphere environments. The closest 16S and gyrB matches to strain F6_2S_P_1T are to C. rhizosphaerae with 98.84 and 93.99% sequence similarity, while a core single-copy gene phylogeny from all publicly available Cohnella genomes places it as more closely related to C. ginsengisoli. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values to any described Cohnella species are <89 and <22%, respectively. The major fatty acids for strain F6_2S_P_1T are anteiso-C15:0 (51.7%), iso-C16:0 (23.1%), and iso-C15:0 (10.5%), and it is able to metabolize a wide range of carbon compounds. Given the results of the ANI and dDDH analyses, this ISS strain is a novel species within the genus Cohnella for which we propose the name Cohnella hashimotonis, with the type strain F6_2S_P_1T (=NRRL B-65657T and DSMZ 115098T). Because no closely related Cohnella genomes were available, this study generated the whole-genome sequences (WGSs) of the type strains for C. rhizosphaerae and C. ginsengisoli. Phylogenetic and pangenomic analysis reveals that F6_2S_P_1T, C. rhizosphaerae, and C. ginsengisoli, along with two uncharacterized Cohnella strains, possess a shared set of 332 gene clusters which are not shared with any other WGS of Cohnella species, and form a distinct clade branching off from C. nanjingensis. Functional traits were predicted for the genomes of strain F6_2S_P_1T and other members of this clade.
Keywords	International Space Station (ISS) ; novel bacteria ; Cohnella ; space biology ; pangenome analysis ; Microbiology ; QR1-502
Subject code	572
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Phylogenomics, phenotypic, and functional traits of five novel (Earth-derived) bacterial species isolated from the International Space Station and their prevalence in metagenomes

Anna C. Simpson / Pratyay Sengupta / Flora Zhang / Asif Hameed / Ceth W. Parker / Nitin K. Singh / Georgios Miliotis / Punchappady D. Rekha / Karthik Raman / Christopher E. Mason / Kasthuri Venkateswaran

Scientific Reports, Vol 13, Iss 1, Pp 1-

2023 Volume 22

Abstract: Abstract With the advent of long-term human habitation in space and on the moon, understanding how the built environment microbiome of space habitats differs from Earth habitats, and how microbes survive, proliferate and spread in space conditions, is ... ...

Abstract	Abstract With the advent of long-term human habitation in space and on the moon, understanding how the built environment microbiome of space habitats differs from Earth habitats, and how microbes survive, proliferate and spread in space conditions, is becoming more important. The microbial tracking mission series has been monitoring the microbiome of the International Space Station (ISS) for almost a decade. During this mission series, six unique strains of Gram-stain-positive bacteria, including two spore-forming and three non-spore-forming species, were isolated from the environmental surfaces of the ISS. The analysis of their 16S rRNA gene sequences revealed > 99% similarities with previously described bacterial species. To further explore their phylogenetic affiliation, whole genome sequencing was undertaken. For all strains, the gyrB gene exhibited < 93% similarity with closely related species, which proved effective in categorizing these ISS strains as novel species. Average nucleotide identity and digital DNA–DNA hybridization values, when compared to any known bacterial species, were < 94% and <50% respectively for all species described here. Traditional biochemical tests, fatty acid profiling, polar lipid, and cell wall composition analyses were performed to generate phenotypic characterization of these ISS strains. A study of the shotgun metagenomic reads from the ISS samples, from which the novel species were isolated, showed that only 0.1% of the total reads mapped to the novel species, supporting the idea that these novel species are rare in the ISS environments. In-depth annotation of the genomes unveiled a variety of genes linked to amino acid and derivative synthesis, carbohydrate metabolism, cofactors, vitamins, prosthetic groups, pigments, and protein metabolism. Further analysis of these ISS-isolated organisms revealed that, on average, they contain 46 genes associated with virulence, disease, and defense. The main predicted functions of these genes are: conferring resistance to ...
Keywords	Medicine ; R ; Science ; Q
Subject code	590
Language	English
Publishing date	2023-11-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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