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Article ; Online: Advantages of grass-grain crop rotations due to prolonged use of clover-alfalfa-timothy mixture

A. K. Svechnikov

Аграрная наука Евро-Северо-Востока, Vol 21, Iss 6, Pp 752-

2020 Volume 763

Abstract: It is known that significant saving of nitrogen fertilizers are due to perennial legume-cereal grasses use in crop rotations. From 2013 to 2018in the Mari El Republic six-field grass-grain fodder crop rotations were compared on sod-podzolic soils with a ... ...

Abstract	It is known that significant saving of nitrogen fertilizers are due to perennial legume-cereal grasses use in crop rotations. From 2013 to 2018in the Mari El Republic six-field grass-grain fodder crop rotations were compared on sod-podzolic soils with a very high level of phosphorus and potassium. In the third rotation their productivity and bioenergetic efficiency, changes in several important soil fertility indicators, and crud protein content in the produced fodder were evaluated. The main difference between the crop rotations was based on the duration of the clover-alfalfa-timothy grass mixture (CAG) use: from one year to three years. In given experiment there was also studied the effect of mineral nitrogen (variants N0, N60) against Р60К60 background on the yield of crop rotations. During six years, there was no significant soil acidification in the variants. Each additional year of clover-alfalfa-timothy grass mixture use raised the energy efficiency ratio of crop rotations by 24-47 % (from 1.13-1.24 by one-year use to 2.08-2.25 by three years of use). Three-year CAG use as compared with one- and two-years has given to the crop rotation significant advantages in energy efficiency (up to two times) and productivity (approximately 40-80 %) of cultivated crops. After refusing to apply nitrogen fertilizations in such crop rotation, average crop productivity, soil humus and nitrogen content in the soil were better preserved. The average crude protein content in dry matter of the obtained fodder increased from 12.7 % to 14.6 % when prolonging theca use up to two years. The average energy value of the yield per rotation was recorded low (8.4-8.7 MJ/kg) and did not depend on the studied factors.
Keywords	perennial grasses ; total nitrogen ; humus ; energy efficiency ratio ; crude protein ; Agriculture ; S
Subject code	630
Language	Russian
Publishing date	2020-12-01T00:00:00Z
Publisher	Federal Agricultural Research Center of the North-East named N.V. Rudnitsky
Document type	Article ; Online
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Article: Advantages of grass-grain crop rotations due to prolonged use of clover-alfalfa-timothy mixture

A. K. Svechnikov

Agrarnaâ nauka Evro-Severo-Vostoka. 2020 Dec., v. 21, no. 6

2020

Abstract	It is known that significant saving of nitrogen fertilizers are due to perennial legume-cereal grasses use in crop rotations. From 2013 to 2018in the Mari El Republic six-field grass-grain fodder crop rotations were compared on sod-podzolic soils with a very high level of phosphorus and potassium. In the third rotation their productivity and bioenergetic efficiency, changes in several important soil fertility indicators, and crud protein content in the produced fodder were evaluated. The main difference between the crop rotations was based on the duration of the clover-alfalfa-timothy grass mixture (CAG) use: from one year to three years. In given experiment there was also studied the effect of mineral nitrogen (variants N0, N60) against Р60К60 background on the yield of crop rotations. During six years, there was no significant soil acidification in the variants. Each additional year of clover-alfalfa-timothy grass mixture use raised the energy efficiency ratio of crop rotations by 24-47 % (from 1.13-1.24 by one-year use to 2.08-2.25 by three years of use). Three-year CAG use as compared with one- and two-years has given to the crop rotation significant advantages in energy efficiency (up to two times) and productivity (approximately 40-80 %) of cultivated crops. After refusing to apply nitrogen fertilizations in such crop rotation, average crop productivity, soil humus and nitrogen content in the soil were better preserved. The average crude protein content in dry matter of the obtained fodder increased from 12.7 % to 14.6 % when prolonging theca use up to two years. The average energy value of the yield per rotation was recorded low (8.4-8.7 MJ/kg) and did not depend on the studied factors.
Keywords	crop rotation ; crude protein ; energy efficiency ; forage ; grasses ; humus ; nitrogen ; nitrogen content ; phosphorus ; potassium ; soil ; soil acidification ; soil fertility
Language	English
Dates of publication	2020-12
Size	p. 752-763.
Publishing place	Federal Agricultural Research Center of the North-East named N.V. Rudnitsky
Document type	Article
ISSN	2500-1396
DOI	10.30766/2072-9081.2020.21.6.752-763
Database	NAL-Catalogue (AGRICOLA)

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Article: Accumulation of root-stubble residues and nutrients in feed crop rotations

A. K. Svechnikov

Agrarnaâ nauka Evro-Severo-Vostoka. 2019 Dec., v. 20, no. 6

2019

Abstract: From 2001 to 2018 in Mari El Republic six-field fodder crop rotations including 1-3 fields of legume-cereal grasses were studied. The amount of plowed root-crop residues and nutrients entering the soil was assessed at different levels of minerals ... ...

Abstract	From 2001 to 2018 in Mari El Republic six-field fodder crop rotations including 1-3 fields of legume-cereal grasses were studied. The amount of plowed root-crop residues and nutrients entering the soil was assessed at different levels of minerals application (N60P60K60 and N90P90K90 in the 1st and 2 nd rotations, N60P60K60 и P60K60 in the 3rd rotation). During the research the total nitrogen content in sod-podzolic soil increased from 0.15% to 0.28%, the humus content raised from 1.82% to 2.53%. The largest amount of root-crop residues nutrients was plowed into 0 -20 cm soil layer during the fodder crop rotation at the 1-year use of the perennial grasses. On the average, it received 35.2 t/ha of dry matter, 560 kg/ha of nitrogen, 231 kg/ha of phosphorus and 338 kg/ha of potassium over 3 rotations. An increase in supply of soils with nitrogen and humus in the 3rd rotation raised the plant residues and nutrients accumulation to 49.9 t/ha, 821 kg/ha, 321 kg and 496 kg/ha, respectively. No significant differences were found between the fertilization backgrounds. In the structure of studied crop rotations cloveralfalfa-timothy grass mixture has made the greatest contribution to in the replenishment of nutrient elements by plowing up the root-crop residues: 10.1 tons of dry matter, 199 kg of nitrogen, 89 kg of phosphorus and 115 kg/ha of potassium. Using postcut mustard after winter rye provided almost the same amount of plowed root-crop residues as clover-alfalfa-timothy grass mixture.Conflict of interest: the authors stated that there was no conflict of interest.
Keywords	crop rotation ; forage ; humus ; nitrogen ; phosphorus ; plowing ; potassium ; soil ; total nitrogen ; winter rye
Language	English
Dates of publication	2019-12
Size	p. 613-622.
Publishing place	Federal Agricultural Research Center of the North-East named N.V. Rudnitsky
Document type	Article
ISSN	2500-1396
DOI	10.30766/2072-9081.2019.20.6.613-622
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Accumulation of root-stubble residues and nutrients in feed crop rotations

A. K. Svechnikov

Аграрная наука Евро-Северо-Востока, Vol 20, Iss 6, Pp 613-

2019 Volume 622

Abstract	From 2001 to 2018 in Mari El Republic six-field fodder crop rotations including 1-3 fields of legume-cereal grasses were studied. The amount of plowed root-crop residues and nutrients entering the soil was assessed at different levels of minerals application (N60P60K60 and N90P90K90 in the 1st and 2 nd rotations, N60P60K60 и P60K60 in the 3rd rotation). During the research the total nitrogen content in sod-podzolic soil increased from 0.15% to 0.28%, the humus content raised from 1.82% to 2.53%. The largest amount of root-crop residues nutrients was plowed into 0 -20 cm soil layer during the fodder crop rotation at the 1-year use of the perennial grasses. On the average, it received 35.2 t/ha of dry matter, 560 kg/ha of nitrogen, 231 kg/ha of phosphorus and 338 kg/ha of potassium over 3 rotations. An increase in supply of soils with nitrogen and humus in the 3rd rotation raised the plant residues and nutrients accumulation to 49.9 t/ha, 821 kg/ha, 321 kg and 496 kg/ha, respectively. No significant differences were found between the fertilization backgrounds. In the structure of studied crop rotations cloveralfalfa-timothy grass mixture has made the greatest contribution to in the replenishment of nutrient elements by plowing up the root-crop residues: 10.1 tons of dry matter, 199 kg of nitrogen, 89 kg of phosphorus and 115 kg/ha of potassium. Using postcut mustard after winter rye provided almost the same amount of plowed root-crop residues as clover-alfalfa-timothy grass mixture.Conflict of interest: the authors stated that there was no conflict of interest.
Keywords	crop rotation crops ; perennial grasses ; rotation ; nutritional elements ; dry matter ; Agriculture ; S
Subject code	580
Language	Russian
Publishing date	2019-12-01T00:00:00Z
Publisher	Federal Agricultural Research Center of the North-East named N.V. Rudnitsky
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Adipocytokines may delay pubertal maturation of human Sertoli cells.

Wagner, I V / Yango, P / Svechnikov, K / Tran, N D / Söder, O

Reproduction, fertility, and development

2019 Volume 31, Issue 8, Page(s) 1395–1400

Abstract: Reproduction is an important target of obesity complications, including adverse effects on spermatogenesis and steroidogenesis. Adipocytokines are key mediators in various complications of obesity. Our aim was to study the potential of adipocytokines to ... ...

Abstract	Reproduction is an important target of obesity complications, including adverse effects on spermatogenesis and steroidogenesis. Adipocytokines are key mediators in various complications of obesity. Our aim was to study the potential of adipocytokines to affect Sertoli cell function, which is crucial for spermatogenesis, and possibly link these findings to the observed attenuation of spermatogenesis in obese males. Testicular biopsies were obtained from healthy donors. Highly purified adult human Sertoli cells (HSCs) were isolated by fluorescence-activated cell sorting. Cells were cultured and exposed to different concentrations of adipocytokines (10-1000ngmL-1 ) for 2-7 days. Expression of selected Sertoli cell genes was quantified by quantitative polymerase chain reaction. Long-term treatment (7 days) of HSCs with higher concentrations of chemerin, irisin, nicotinamide phosphoribosyltransferase (Nampt), resistin and progranulin significantly suppressed FSH receptor expression (by 79%, 83%, 64%, 71% and 26% respectively; P P invitro , may negatively affect Sertoli cell maturation and retain these cells in a more prepubertal stage. This could negatively affect testis function and add to fertility problems in obese adults.
Language	English
Publishing date	2019-04-17
Publishing country	Australia
Document type	Journal Article
ZDB-ID	1019913-5
ISSN	1448-5990 ; 1031-3613
ISSN (online)	1448-5990
ISSN	1031-3613
DOI	10.1071/RD18487
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Zs.A 2756: Show issues

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Article: Productivity of fodder crop rotations depending on the nitrogen fertilizers

A. K. Svechnikov / V. M. Izmestyev / E. A. Sokolova

Agrarnaâ nauka Evro-Severo-Vostoka. 2018 Apr., v. 63, no. 2

2018

Abstract: Legumes in the structure of fodder crop rotations can cause reducing the efficiency of nitrogen fertilizer application on sod-podzolic soil. A comparative assessment of the productivity of six-field fodder crop rotations with different field number of ... ...

Abstract	Legumes in the structure of fodder crop rotations can cause reducing the efficiency of nitrogen fertilizer application on sod-podzolic soil. A comparative assessment of the productivity of six-field fodder crop rotations with different field number of legume-cereal grasses was carried out on the background of mineral nitrogen application (Р60К60, Ж0Р60К60). The research was carried out in 2013-2017 in crop rotations with 16%, 33% and 50 % legume-cereal grasses saturation in the Mari El Republic. It was noted that one-year extension of period for using perennial legume-cereal grasses in the fodder crop rotations under study was followed by increase in dry matter collection by 1.1-2.1 t/ha, exchange energy - by 6.7-16.5 GJ/ha, and crude protein - by about 2.3-3.7 C/ha. Continuous application of mineral nitrogen led to increasing the productivity of fodder crop rotations with legume-cereal grasses by 9-21%. In crop rotations, target annual and winter crops ensured a significant increase in productivity, with the exception of annual grasses with perennial grasses undersowing in 2013. The greatest increase in productivity was noted in the first crop rotation, where a long-term agrocenosis had been used for 1 year only. The ammonium nitrate application resulted in increase in crude protein collection more than in other productivity indicators of fodder crop rotations target crops.
Keywords	ammonium nitrate ; crop rotation ; crude protein ; energy ; fertilizer application ; forage ; nitrogen ; nitrogen fertilizers ; soil
Language	English
Dates of publication	2018-04
Size	p. 69-73.
Publishing place	Federal Agricultural Research Center of the North-East named N.V. Rudnitsky
Document type	Article
ISSN	2500-1396
DOI	10.30766/2072-9081.2018.63.2.69-73
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Mono-2-ethylhexyl phthalate stimulates androgen production but suppresses mitochondrial function in mouse leydig cells with different steroidogenic potential.

Savchuk, Iuliia / Söder, Olle / Svechnikov, Konstantin

Toxicological sciences : an official journal of the Society of Toxicology

2015 Volume 145, Issue 1, Page(s) 149–156

Abstract: Numerous studies have reported on testicular toxicity of phthalates in different experimental paradigms and showed that Leydig cells (LCs) were one of the main targets of phthalate actions. Adverse effects of phthalates on LCs steroidogenesis have been ... ...

Abstract	Numerous studies have reported on testicular toxicity of phthalates in different experimental paradigms and showed that Leydig cells (LCs) were one of the main targets of phthalate actions. Adverse effects of phthalates on LCs steroidogenesis have been attributed to their metabolites, monophthalates. This study focuses on investigation whether LCs responsiveness to monophthalates action is associated with their potential to produce androgens. We found that of 3 monophthalates investigated [ie, mono-2-ethylhexyl phthalate (MEHP), mono-n-butyl phthalate, and mono-n-benzyl phthalate] only MEHP caused biological effects on the mouse LCs function. This monophthalate stimulated basal steroidogenesis associated with upregulation of StAR protein expression with no effect on hCG-stimulated androgen production by LCs from CBA/Lac and C57BL/6j mouse genotypes were observed. Further, MEHP attenuated ATP production and increased superoxide generation by both phenotypes of mouse LCs that indicated on mitochondrial dysfunction induced by the monophthalate. All together, our data indicate that MEHP-mediated stimulation of steroidogenesis and perturbation in mitochondrial function are not associated with the capacity of the LCs to synthesize androgens. We suggest that this effect of MEHP observed in LCs of rodent origin needs to be taken into consideration in analysis of earlier start of puberty in boys and may highlight a possible influence of phthalates on reproductive health in males.
MeSH term(s)	Androgens/biosynthesis ; Animals ; Diethylhexyl Phthalate/analogs & derivatives ; Diethylhexyl Phthalate/toxicity ; Leydig Cells/drug effects ; Leydig Cells/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Inbred CBA ; Mitochondria/drug effects ; Mitochondria/metabolism ; Phosphoproteins/metabolism ; Steroids/biosynthesis
Chemical Substances	Androgens ; Phosphoproteins ; Steroids ; steroidogenic acute regulatory protein ; Diethylhexyl Phthalate (C42K0PH13C) ; mono-(2-ethylhexyl)phthalate (FU2EWB60RT)
Language	English
Publishing date	2015-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1420885-4
ISSN	1096-0929 ; 1096-6080
ISSN (online)	1096-0929
ISSN	1096-6080
DOI	10.1093/toxsci/kfv042
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Article ; Online: Adipose Tissue is a Potential Source of Hyperandrogenism in Obese Female Rats.

Wagner, Isabel Viola / Sahlin, Lena / Savchuk, Iuliia / Klöting, Nora / Svechnikov, Konstantin / Söder, Olle

Obesity (Silver Spring, Md.)

2018 Volume 26, Issue 7, Page(s) 1161–1167

Abstract: Objective: Obesity in females is often associated with metabolic complications and hyperandrogenism, but the sources of androgens are not completely understood. Therefore, this study investigated whether adipose tissue could be a source of androgens ... ...

Abstract	Objective: Obesity in females is often associated with metabolic complications and hyperandrogenism, but the sources of androgens are not completely understood. Therefore, this study investigated whether adipose tissue could be a source of androgens promoting hyperandrogenism development in obese female rats. Methods: Gene expression of steroidogenic enzymes and testosterone levels were determined in periovarian and inguinal adipose tissue and in the supernatant of cultured preadipocytes and adipocytes. The conversion of pregnenolone to androgens was analyzed by thin-layer chromatography. Results: Substantial amounts of testosterone in adipose tissue (25-153 ng/g tissue) and in the supernatant of adipocytes (0.33-0.69 ng/ten thousand cells]) were found. StAR and steroidogenic enzymes encoded by genes including Cyp11A1, Cyp17A1, Cyp19, Hsd3b2, Hsd17b3, and Srd5a2 were expressed in adipose tissue and cultured cells. Thin layer chromatography data revealed that preadipocytes and adipocytes were able to convert pregnenolone to testosterone. Higher levels for all steroidogenic enzymes were found in both depots of obese animals compared with lean animals, with significantly higher levels in inguinal tissue. Conclusions: The whole steroidogenic machinery and capacity for testosterone biosynthesis were found in fat depots of female rats. These findings support the hypothesis that adipose tissue may contribute substantially to the hyperandrogenism in female obesity.
MeSH term(s)	Adipocytes/metabolism ; Adipose Tissue/metabolism ; Adipose Tissue/physiology ; Androgens/metabolism ; Animals ; Cells, Cultured ; Female ; Gene Expression ; Hyperandrogenism/etiology ; Hyperandrogenism/metabolism ; Lipogenesis/physiology ; Obesity/complications ; Obesity/metabolism ; Obesity/pathology ; Rats ; Rats, Inbred Lew ; Rats, Sprague-Dawley ; Testosterone/metabolism
Chemical Substances	Androgens ; Testosterone (3XMK78S47O)
Language	English
Publishing date	2018-06-14
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2230457-5
ISSN	1930-739X ; 1071-7323 ; 1930-7381
ISSN (online)	1930-739X
ISSN	1071-7323 ; 1930-7381
DOI	10.1002/oby.22198
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Article ; Online: Steroidogenic potential of human fetal kidney at early gestational age.

Savchuk, I / Morvan, M L / Antignac, J P / Kurek, M / Le Bizec, B / Söder, O / Svechnikov, K

Steroids

2019 Volume 149, Page(s) 108417

Abstract: Steroidogenic potential of the human fetal kidney (hFK) at the end of first trimester is poorly investigated. Little is known about the ontogeny of steroidogenic enzymes and activities of steroidogenic pathways in the hFK at early pregnancy. Our aim was ... ...

Abstract	Steroidogenic potential of the human fetal kidney (hFK) at the end of first trimester is poorly investigated. Little is known about the ontogeny of steroidogenic enzymes and activities of steroidogenic pathways in the hFK at early pregnancy. Our aim was to explore steroidogenesis and the expression of steroidogenic enzymes in the hFK at gestational weeks (GW) 9-12. Steroids in the hFK were analyzed by gas chromatography/coupled to tandem mass spectrometry. The expression of steroidogenic enzymes in the hFK at GW 9-12 was investigated by qPCR, automated Western blotting and immunohistochemistry. We observed that the hFK produced substantial amount of steroids of the Δ
MeSH term(s)	Female ; Fetus/metabolism ; Gene Expression Regulation, Enzymologic ; Gestational Age ; Humans ; Kidney/embryology ; Kidney/metabolism ; Pregnancy ; Steroids/biosynthesis
Chemical Substances	Steroids
Language	English
Publishing date	2019-05-28
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80312-1
ISSN	1878-5867 ; 0039-128X
ISSN (online)	1878-5867
ISSN	0039-128X
DOI	10.1016/j.steroids.2019.05.009
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Article ; Online: Productivity of fodder crop rotations depending on the nitrogen fertilizers

A. K. Svechnikov / V. M. Izmestyev / E. A. Sokolova

Аграрная наука Евро-Северо-Востока, Vol 63, Iss 2, Pp 69-

2018 Volume 73

Abstract	Legumes in the structure of fodder crop rotations can cause reducing the efficiency of nitrogen fertilizer application on sod-podzolic soil. A comparative assessment of the productivity of six-field fodder crop rotations with different field number of legume-cereal grasses was carried out on the background of mineral nitrogen application (Р60К60, Ж0Р60К60). The research was carried out in 2013-2017 in crop rotations with 16%, 33% and 50 % legume-cereal grasses saturation in the Mari El Republic. It was noted that one-year extension of period for using perennial legume-cereal grasses in the fodder crop rotations under study was followed by increase in dry matter collection by 1.1-2.1 t/ha, exchange energy - by 6.7-16.5 GJ/ha, and crude protein - by about 2.3-3.7 C/ha. Continuous application of mineral nitrogen led to increasing the productivity of fodder crop rotations with legume-cereal grasses by 9-21%. In crop rotations, target annual and winter crops ensured a significant increase in productivity, with the exception of annual grasses with perennial grasses undersowing in 2013. The greatest increase in productivity was noted in the first crop rotation, where a long-term agrocenosis had been used for 1 year only. The ammonium nitrate application resulted in increase in crude protein collection more than in other productivity indicators of fodder crop rotations target crops.
Keywords	fodder crop rotation ; legume-cereal ; nitrogen ; productivity ; dry matter ; exchange energy ; crude protein ; Agriculture ; S
Subject code	571
Language	Russian
Publishing date	2018-04-01T00:00:00Z
Publisher	Federal Agricultural Research Center of the North-East named N.V. Rudnitsky
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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