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  1. Article ; Online: GALDAR: A genetically encoded galactose sensor for visualizing sugar metabolism in vivo.

    Sakizli, Uğurcan / Takano, Tomomi / Yoo, Sa Kan

    PLoS biology

    2024  Volume 22, Issue 3, Page(s) e3002549

    Abstract: Sugar metabolism plays a pivotal role in sustaining life. Its dynamics within organisms is less understood compared to its intracellular metabolism. Galactose, a hexose stereoisomer of glucose, is a monosaccharide transported via the same transporters ... ...

    Abstract Sugar metabolism plays a pivotal role in sustaining life. Its dynamics within organisms is less understood compared to its intracellular metabolism. Galactose, a hexose stereoisomer of glucose, is a monosaccharide transported via the same transporters with glucose. Galactose feeds into glycolysis and regulates protein glycosylation. Defects in galactose metabolism are lethal for animals. Here, by transgenically implementing the yeast galactose sensing system into Drosophila, we developed a genetically encoded sensor, GALDAR, which detects galactose in vivo. Using this heterologous system, we revealed dynamics of galactose metabolism in various tissues. Notably, we discovered that intestinal stem cells do not uptake detectable levels of galactose or glucose. GALDAR elucidates the role for galactokinase in metabolism of galactose and a transition of galactose metabolism during the larval period. This work provides a new system that enables analyses of in vivo sugar metabolism.
    MeSH term(s) Animals ; Galactose/metabolism ; Glycolysis/genetics ; Glycosylation ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Drosophila/metabolism ; Glucose/metabolism
    Chemical Substances Galactose (X2RN3Q8DNE) ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 2024-03-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2126776-5
    ISSN 1545-7885 ; 1544-9173
    ISSN (online) 1545-7885
    ISSN 1544-9173
    DOI 10.1371/journal.pbio.3002549
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  2. Article: Immunodominant B-Cell Linear Epitope on the VP1 P Domain of a Feline Norovirus Cat Model.

    Takano, Tomomi / Ryu, Mizuki / Doki, Tomoyoshi / Kusuhara, Hajime

    Pathogens (Basel, Switzerland)

    2022  Volume 11, Issue 7

    Abstract: Norovirus (NoV) infection remains a major public health concern worldwide. Appropriate animal models are essential for the development of effective NoV vaccines. We previously established the feline NoV (FNoV)-cat model as a surrogate animal model for ... ...

    Abstract Norovirus (NoV) infection remains a major public health concern worldwide. Appropriate animal models are essential for the development of effective NoV vaccines. We previously established the feline NoV (FNoV)-cat model as a surrogate animal model for human NoV infection. In the present study, we analyzed the B-cell linear epitope in the P domain of FNoV to confirm the basic immunological features of the FNoV-cat model. B-cell linear epitopes were present in the P2 subdomain. We compared antibody levels to peptides containing the B-cell linear epitope (P-10) in three FNoV-infected cats with time-course changes in viral load and symptom scoring. After FNoV infection, viral shedding and clinical symptoms were shown to improve by elevated levels of antibodies against P-10 in the plasma. This report provides important information for understanding NoV infections in humans and cats.
    Language English
    Publishing date 2022-06-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens11070731
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  3. Article ; Online: In vitro antiviral effects of GS-441524 and itraconazole combination against feline infectious peritonitis virus.

    Doki, Tomoyoshi / Takahashi, Ken / Hasegawa, Nobuhisa / Takano, Tomomi

    Research in veterinary science

    2022  Volume 144, Page(s) 27–33

    Abstract: Feline infectious peritonitis virus (FIPV: virulent feline coronavirus) causes a fatal disease called feline infectious peritonitis (FIP) in wild and domestic cat species. Recent studies identified several antiviral drugs that are effective against FIPV. ...

    Abstract Feline infectious peritonitis virus (FIPV: virulent feline coronavirus) causes a fatal disease called feline infectious peritonitis (FIP) in wild and domestic cat species. Recent studies identified several antiviral drugs that are effective against FIPV. Drug combination is one of the important strategies in the development of novel treatments for viral infections. GS-441524, a nucleoside analog, and itraconazole, a triazole antifungal drug, have been reported that have antiviral effect against FIPV. This study aims to investigate whether the combination of GS-441524 and itraconazole has synergic antiviral effect against FIPV. The antiviral effect was measured by plaque reduction assay using felis catus whole fatus-4 cell. The plaque reduction of GS-441524 against type I FIPVs increased as the concentration of itraconazole increased. The similar result was obtained for type II FIPV. In addition, the calculated combination index (CI) demonstrated that there was a strong synergy between GS-441524 and itraconazole. It is concluded that the combination of GS-441524 and itraconazole may enhance the individual effect of each drug against replication of type I FIPVs and may contribute to development more effective treatment strategy for FIP.
    MeSH term(s) Adenosine/analogs & derivatives ; Animals ; Antiviral Agents/pharmacology ; Antiviral Agents/therapeutic use ; Cat Diseases/drug therapy ; Cats ; Coronavirus, Feline ; Feline Infectious Peritonitis/drug therapy ; Itraconazole/pharmacology ; Itraconazole/therapeutic use
    Chemical Substances Antiviral Agents ; GS-441524 (1BQK176DT6) ; Itraconazole (304NUG5GF4) ; Adenosine (K72T3FS567)
    Language English
    Publishing date 2022-01-07
    Publishing country England
    Document type Journal Article
    ZDB-ID 840961-4
    ISSN 1532-2661 ; 0034-5288
    ISSN (online) 1532-2661
    ISSN 0034-5288
    DOI 10.1016/j.rvsc.2022.01.005
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  4. Article: Immunodominant B-Cell Linear Epitope on the VP1 P Domain of a Feline Norovirus Cat Model

    Takano, Tomomi / Ryu, Mizuki / Doki, Tomoyoshi / Kusuhara, Hajime

    Pathogens. 2022 June 27, v. 11, no. 7

    2022  

    Abstract: Norovirus (NoV) infection remains a major public health concern worldwide. Appropriate animal models are essential for the development of effective NoV vaccines. We previously established the feline NoV (FNoV)-cat model as a surrogate animal model for ... ...

    Abstract Norovirus (NoV) infection remains a major public health concern worldwide. Appropriate animal models are essential for the development of effective NoV vaccines. We previously established the feline NoV (FNoV)-cat model as a surrogate animal model for human NoV infection. In the present study, we analyzed the B-cell linear epitope in the P domain of FNoV to confirm the basic immunological features of the FNoV-cat model. B-cell linear epitopes were present in the P2 subdomain. We compared antibody levels to peptides containing the B-cell linear epitope (P-10) in three FNoV-infected cats with time-course changes in viral load and symptom scoring. After FNoV infection, viral shedding and clinical symptoms were shown to improve by elevated levels of antibodies against P-10 in the plasma. This report provides important information for understanding NoV infections in humans and cats.
    Keywords B-lymphocytes ; Norovirus ; animal models ; antibodies ; cats ; epitopes ; humans ; peptides ; public health ; viral load
    Language English
    Dates of publication 2022-0627
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens11070731
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  5. Article ; Online: Immunodominant B-Cell Linear Epitope on the VP1 P Domain of a Feline Norovirus Cat Model

    Tomomi Takano / Mizuki Ryu / Tomoyoshi Doki / Hajime Kusuhara

    Pathogens, Vol 11, Iss 731, p

    2022  Volume 731

    Abstract: Norovirus (NoV) infection remains a major public health concern worldwide. Appropriate animal models are essential for the development of effective NoV vaccines. We previously established the feline NoV (FNoV)-cat model as a surrogate animal model for ... ...

    Abstract Norovirus (NoV) infection remains a major public health concern worldwide. Appropriate animal models are essential for the development of effective NoV vaccines. We previously established the feline NoV (FNoV)-cat model as a surrogate animal model for human NoV infection. In the present study, we analyzed the B-cell linear epitope in the P domain of FNoV to confirm the basic immunological features of the FNoV-cat model. B-cell linear epitopes were present in the P2 subdomain. We compared antibody levels to peptides containing the B-cell linear epitope (P-10) in three FNoV-infected cats with time-course changes in viral load and symptom scoring. After FNoV infection, viral shedding and clinical symptoms were shown to improve by elevated levels of antibodies against P-10 in the plasma. This report provides important information for understanding NoV infections in humans and cats.
    Keywords norovirus ; B-cell epitope ; surrogate model ; cat ; Medicine ; R
    Subject code 570
    Language English
    Publishing date 2022-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Increased glycated albumin levels in patients with acromegaly related to glucose fluctuation caused by growth hormone excess but not albumin metabolism.

    Taguchi, Tomomi / Suzuki, Agena / Takano, Koji

    Endocrine journal

    2021  Volume 69, Issue 3, Page(s) 225–233

    Abstract: Acromegaly is often complicated by impaired glucose tolerance. The accuracy of glycated hemoglobin ( ... ...

    Abstract Acromegaly is often complicated by impaired glucose tolerance. The accuracy of glycated hemoglobin (HbA
    MeSH term(s) Acromegaly/complications ; Adult ; Diabetes Mellitus/etiology ; Glucose ; Glycation End Products, Advanced ; Human Growth Hormone ; Humans ; Retrospective Studies ; Serum Albumin
    Chemical Substances Glycation End Products, Advanced ; Serum Albumin ; glycated serum albumin ; Human Growth Hormone (12629-01-5) ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 2021-09-28
    Publishing country Japan
    Document type Journal Article
    ZDB-ID 1151918-6
    ISSN 1348-4540 ; 0918-8959
    ISSN (online) 1348-4540
    ISSN 0918-8959
    DOI 10.1507/endocrj.EJ21-0439
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  7. Article: In Vivo Antiviral Effects of U18666A Against Type I Feline Infectious Peritonitis Virus.

    Doki, Tomoyoshi / Tarusawa, Tomoyo / Hohdatsu, Tsutomu / Takano, Tomomi

    Pathogens (Basel, Switzerland)

    2020  Volume 9, Issue 1

    Abstract: Background: ...

    Abstract Background:
    Keywords covid19
    Language English
    Publishing date 2020-01-18
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens9010067
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  8. Article ; Online: Oncogenic stress-induced Netrin is a humoral signaling molecule that reprograms systemic metabolism in Drosophila.

    Okada, Morihiro / Takano, Tomomi / Ikegawa, Yuko / Ciesielski, Hanna / Nishida, Hiroshi / Yoo, Sa Kan

    The EMBO journal

    2023  Volume 42, Issue 12, Page(s) e111383

    Abstract: Cancer exerts pleiotropic, systemic effects on organisms, leading to health deterioration and eventually to organismal death. How cancer induces systemic effects on remote organs and the organism itself still remains elusive. Here we describe a role for ... ...

    Abstract Cancer exerts pleiotropic, systemic effects on organisms, leading to health deterioration and eventually to organismal death. How cancer induces systemic effects on remote organs and the organism itself still remains elusive. Here we describe a role for NetrinB (NetB), a protein with a particularly well-characterized role as a tissue-level axon guidance cue, in mediating oncogenic stress-induced organismal, metabolic reprogramming as a systemic humoral factor. In Drosophila, Ras-induced dysplastic cells upregulate and secrete NetB. Inhibition of either NetB from the transformed tissue or its receptor in the fat body suppresses oncogenic stress-induced organismal death. NetB from the dysplastic tissue remotely suppresses carnitine biosynthesis in the fat body, which is critical for acetyl-CoA generation and systemic metabolism. Supplementation of carnitine or acetyl-CoA ameliorates organismal health under oncogenic stress. This is the first identification, to our knowledge, of a role for the Netrin molecule, which has been studied extensively for its role within tissues, in humorally mediating systemic effects of local oncogenic stress on remote organs and organismal metabolism.
    MeSH term(s) Animals ; Drosophila/metabolism ; Netrins/metabolism ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Acetyl Coenzyme A/metabolism ; Signal Transduction ; Axons/metabolism ; Nerve Growth Factors/metabolism
    Chemical Substances Netrins ; Drosophila Proteins ; Acetyl Coenzyme A (72-89-9) ; NetB protein, Drosophila ; Nerve Growth Factors
    Language English
    Publishing date 2023-05-04
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 586044-1
    ISSN 1460-2075 ; 0261-4189
    ISSN (online) 1460-2075
    ISSN 0261-4189
    DOI 10.15252/embj.2022111383
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  9. Article ; Online: Prophylactic administration of ivermectin attenuates SARS-CoV-2 induced disease in a Syrian Hamster Model.

    Uematsu, Takayuki / Takano, Tomomi / Matsui, Hidehito / Kobayashi, Noritada / Ōmura, Satoshi / Hanaki, Hideaki

    The Journal of antibiotics

    2023  Volume 76, Issue 8, Page(s) 481–488

    Abstract: COVID-19, caused by SARS-CoV-2 infection, is currently among the most important public health concerns worldwide. Although several effective vaccines have been developed, there is an urgent clinical need for effective pharmaceutical treatments for ... ...

    Abstract COVID-19, caused by SARS-CoV-2 infection, is currently among the most important public health concerns worldwide. Although several effective vaccines have been developed, there is an urgent clinical need for effective pharmaceutical treatments for treatment of COVID-19. Ivermectin, a chemical derivative of avermectin produced by Streptomyces avermitilis, is a macrocyclic lactone with antiparasitic activity. Recent studies have shown that ivermectin inhibits SARS-CoV-2 replication in vitro. In the present study, we investigated the in vivo effects of ivermectin in a hamster model of SARS-CoV-2 infection. The results of the present study demonstrate oral administration of ivermectin prior to SARS-CoV-2 infection in hamsters was associated with decreased weight loss and pulmonary inflammation. In addition, the administration of ivermectin reduced pulmonary viral titers and mRNA expression level of pro-inflammatory cytokines associated with severe COVID-19 disease. The administration of ivermectin rapidly induced the production of virus-specific neutralizing antibodies in the late stage of viral infection. Zinc concentrations leading to immune quiescence were also significantly higher in the lungs of ivermectin-treated hamsters compared to controls. These results indicate that ivermectin may have efficacy in reducing the development and severity of COVID-19 by affecting host immunity in a hamster model of SARS-CoV-2 infection.
    MeSH term(s) Cricetinae ; Animals ; COVID-19 ; Mesocricetus ; SARS-CoV-2 ; Ivermectin/pharmacology ; Ivermectin/therapeutic use ; Lung
    Chemical Substances Ivermectin (70288-86-7)
    Language English
    Publishing date 2023-04-25
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 390800-8
    ISSN 1881-1469 ; 0021-8820 ; 0368-3532
    ISSN (online) 1881-1469
    ISSN 0021-8820 ; 0368-3532
    DOI 10.1038/s41429-023-00623-0
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  10. Article: Possible Antiviral Activity of 5-Aminolevulinic Acid in Feline Infectious Peritonitis Virus (Feline Coronavirus) Infection.

    Takano, Tomomi / Satoh, Kumi / Doki, Tomoyoshi

    Frontiers in veterinary science

    2021  Volume 8, Page(s) 647189

    Abstract: Feline infectious peritonitis (FIP) is a life-threatening infectious disease of cats caused by virulent feline coronavirus (FIP virus: FIPV). For the treatment of FIP, several effective antivirals were recently reported, but many of these are not ... ...

    Abstract Feline infectious peritonitis (FIP) is a life-threatening infectious disease of cats caused by virulent feline coronavirus (FIP virus: FIPV). For the treatment of FIP, several effective antivirals were recently reported, but many of these are not available for practical use. 5-amino levulinic acid (5-ALA) is a low-molecular-weight amino acid synthesized in plant and animal cells. 5-ALA can be synthesized in a large amount, and it is widely applied in the medical and agricultural fields. We hypothesized that 5-ALA inhibits FIPV infection. Therefore, we evaluated its antiviral activity against FIPV in felis catus whole fetus-4 cells and feline primary macrophages. FIPV infection was significantly inhibited by 250 μM 5-ALA. Our study suggested that 5-ALA is applicable for the treatment and prevention of FIPV infection.
    Language English
    Publishing date 2021-02-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2834243-4
    ISSN 2297-1769
    ISSN 2297-1769
    DOI 10.3389/fvets.2021.647189
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