Article ; Online: Dietary Fatty Acid Saturation Modulates Sphingosine-1-Phosphate-Mediated Vascular Function.
2019 Volume 2019, Page(s) 2354274
Abstract: Sphingolipids, modified by dietary fatty acids, are integral components of plasma membrane and caveolae that are also vasoactive compounds. We hypothesized that dietary fatty acid saturation affects vasoconstriction to sphingosine-1-phosphate (S1P) ... ...
| Abstract | Sphingolipids, modified by dietary fatty acids, are integral components of plasma membrane and caveolae that are also vasoactive compounds. We hypothesized that dietary fatty acid saturation affects vasoconstriction to sphingosine-1-phosphate (S1P) through caveolar regulation of rho kinase. Wild type (WT) and caveolin-1-deficient (cav-1 KO) mice which lack vascular caveolae were fed a low-fat diet (LF), 60% high-saturated fat diet (lard, HF), or 60% fat diet with equal amounts of lard and n-3 polyunsaturated menhaden oil (MO). Weight gain of WT on HF and MO diets was similar while markedly blunted in cav-1 KO. Neither high-fat diet affected the expression of cav-1, rho, or rho kinase in arteries from WT. In cav-1 KO, MO increased the vascular expression of rho but had no effect on rho kinase. HF had no effect on rho or rho kinase expression in cav-1 KO. S1P produced a concentration-dependent constriction of gracilis arteries from WT on LF that was reduced with HF and restored to normal with MO. Constriction to S1P was reduced in cav-1 KO and no longer affected by a high-saturated fat diet. Inhibition of rho kinase which reduced constriction to PE independent of diet in arteries from WT and cav-1 KO only reduced constriction to S1P in arteries from WT fed MO. The data suggest that dietary fatty acids modify vascular responses to S1P by a caveolar-dependent mechanism which is enhanced by dietary n-3 polyunsaturated fats. |
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| MeSH term(s) | Animals ; Arteries/metabolism ; Caveolin 1/genetics ; Caveolin 1/metabolism ; Diet, Fat-Restricted ; Diet, High-Fat ; Lysophospholipids/metabolism ; Mice ; Mice, Knockout ; Sphingosine/analogs & derivatives ; Sphingosine/metabolism ; rho-Associated Kinases/metabolism |
| Chemical Substances | Caveolin 1 ; Lysophospholipids ; sphingosine 1-phosphate (26993-30-6) ; rho-Associated Kinases (EC 2.7.11.1) ; Sphingosine (NGZ37HRE42) |
| Language | English |
| Publishing date | 2019-08-25 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 2711897-6 |
| ISSN | 2314-6753 ; 2314-6745 |
| ISSN (online) | 2314-6753 |
| ISSN | 2314-6745 |
| DOI | 10.1155/2019/2354274 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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