Article ; Online: Efficient Vascularization of Kidney Organoids through Intracelomic Transplantation in Chicken Embryos.
Journal of visualized experiments : JoVE
2023 , Issue 192
Abstract: Kidney organoids derived from human induced pluripotent stem cells contain nephron-like structures that resemble those in the adult kidney to a certain degree. Unfortunately, their clinical applicability is hampered by the lack of a functional ... ...
Abstract | Kidney organoids derived from human induced pluripotent stem cells contain nephron-like structures that resemble those in the adult kidney to a certain degree. Unfortunately, their clinical applicability is hampered by the lack of a functional vasculature and consequently limited maturation in vitro. The transplantation of kidney organoids in the celomic cavity of chicken embryos induces vascularization by perfused blood vessels, including the formation of glomerular capillaries, and enhances their maturation. This technique is very efficient, allowing for the transplantation and analysis of large numbers of organoids. This paper describes a detailed protocol for the intracelomic transplantation of kidney organoids in chicken embryos, followed by the injection of fluorescently labeled lectin to stain the perfused vasculature, and the collection of transplanted organoids for imaging analysis. This method can be used to induce and study organoid vascularization and maturation to find clues for enhancing these processes in vitro and improve disease modeling. |
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MeSH term(s) | Chick Embryo ; Adult ; Animals ; Humans ; Chickens ; Induced Pluripotent Stem Cells ; Kidney ; Nephrons ; Kidney Glomerulus ; Neovascularization, Pathologic |
Language | English |
Publishing date | 2023-02-17 |
Publishing country | United States |
Document type | Journal Article ; Video-Audio Media ; Research Support, Non-U.S. Gov't |
ZDB-ID | 2259946-0 |
ISSN | 1940-087X ; 1940-087X |
ISSN (online) | 1940-087X |
ISSN | 1940-087X |
DOI | 10.3791/65090 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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