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  1. Article ; Online: Physical-property-based patterning: simply engineering complex tissues.

    Zlotnick, Hannah M / Stevens, Molly M / Mauck, Robert L

    Trends in biotechnology

    2024  

    Abstract: The field of biofabrication is rapidly expanding with the advent of new technologies and material systems to engineer complex tissues. In this opinion article, we introduce an emerging tissue patterning method, physical-property-based patterning, that ... ...

    Abstract The field of biofabrication is rapidly expanding with the advent of new technologies and material systems to engineer complex tissues. In this opinion article, we introduce an emerging tissue patterning method, physical-property-based patterning, that has strong translational potential given its simplicity and limited dependence on external hardware. Physical-property-based patterning relies solely on the intrinsic density, magnetic susceptibility, or compressibility of an object, its surrounding solution, and the noncontact application of a remote field. We discuss how physical properties can be exploited to pattern objects and design a variety of biologic tissues. Finally, we pose several open questions that, if addressed, could transform the status quo of biofabrication, pushing us one step closer to patterning tissues in situ.
    Language English
    Publishing date 2024-04-24
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 47474-5
    ISSN 1879-3096 ; 0167-7799
    ISSN (online) 1879-3096
    ISSN 0167-7799
    DOI 10.1016/j.tibtech.2024.03.004
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2750: Show issues Location:
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  2. Article ; Online: JOR Spine: Reaping the harvest of a community effort.

    Mauck, Robert L / Alini, Mauro / Sakai, Daisuke

    JOR spine

    2023  Volume 6, Issue 3, Page(s) e1288

    Language English
    Publishing date 2023-09-29
    Publishing country United States
    Document type Journal Article
    ISSN 2572-1143
    ISSN (online) 2572-1143
    DOI 10.1002/jsp2.1288
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  3. Article ; Online: Modeling development using hydrogels.

    Xu, Karen L / Mauck, Robert L / Burdick, Jason A

    Development (Cambridge, England)

    2023  Volume 150, Issue 13

    Abstract: The development of multicellular complex organisms relies on coordinated signaling from the microenvironment, including both biochemical and mechanical interactions. To better understand developmental biology, increasingly sophisticated in vitro systems ... ...

    Abstract The development of multicellular complex organisms relies on coordinated signaling from the microenvironment, including both biochemical and mechanical interactions. To better understand developmental biology, increasingly sophisticated in vitro systems are needed to mimic these complex extracellular features. In this Primer, we explore how engineered hydrogels can serve as in vitro culture platforms to present such signals in a controlled manner and include examples of how they have been used to advance our understanding of developmental biology.
    MeSH term(s) Hydrogels ; Signal Transduction
    Chemical Substances Hydrogels
    Language English
    Publishing date 2023-06-30
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 90607-4
    ISSN 1477-9129 ; 0950-1991
    ISSN (online) 1477-9129
    ISSN 0950-1991
    DOI 10.1242/dev.201527
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    Ub I Zs.183: Show issues Location:
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  4. Article ; Online: Modulating mechanobiology as a therapeutic target for synovial fibrosis to restore joint lubrication.

    Bonnevie, Edward D / Scanzello, Carla R / Mauck, Robert L

    Osteoarthritis and cartilage

    2023  Volume 32, Issue 1, Page(s) 41–51

    Abstract: Objectives: Fibroses are disorders linked to persistence of myofibroblasts due to biochemical (e.g., Transforming growth factor-β) and biophysical cues (e.g., a stiff microenvironment). In the context of osteoarthritis, fibrotic changes in the joint- ... ...

    Abstract Objectives: Fibroses are disorders linked to persistence of myofibroblasts due to biochemical (e.g., Transforming growth factor-β) and biophysical cues (e.g., a stiff microenvironment). In the context of osteoarthritis, fibrotic changes in the joint-lining synovium have been linked with disease progression. The objective of this study was to probe synovial fibroblast mechanobiology and how essential functions (i.e., lubrication) are altered in fibrotic environments.
    Design: Both ex vivo and in vitro synovium models were assessed for fibrotic and lubrication biomarkers to better understand the role of mechanobiology and lubrication. Additionally, in vitro, work on small molecules targeting mechanobiology was assessed.
    Results: Our results indicated that modulating mechanobiology could rescue the fibrotic phenotype instigated by stiffening microenvironment that resulted in altered lubricant expression. A small molecule therapeutic, fasudil, blocked ROCK-mediated contractility and this inhibition of the fibrotic mechano-response of synovial fibroblasts restored proper lubrication function, providing insight into mechanisms of disease progression as well as a new avenue for therapeutic development.
    Conclusion: This study identifies synovial fibrosis as a condition that potentially has joint-wide deficits through inhibiting lubrication. Additionally, modulating mechanobiology (i.e., ROCK-mediated contractility) may pose a potential target for small molecule therapies that can be delivered to the joint space.
    Classification: Applied Biological Sciences.
    MeSH term(s) Humans ; Lubrication ; Fibrosis ; Synovial Membrane/metabolism ; Biophysics ; Disease Progression
    Language English
    Publishing date 2023-10-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 1167809-4
    ISSN 1522-9653 ; 1063-4584
    ISSN (online) 1522-9653
    ISSN 1063-4584
    DOI 10.1016/j.joca.2023.09.012
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3828: Show issues Location:
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  5. Book: Pediatric laryngotracheal reconstruction with tissue-engineered cartilage in a rabbit model

    Jacobs, Ian N. / Redden, Robert A. / Goldberg, Rachel / Hast, Michael / Salowe, Rebecca / Mauck, Robert L. / Doolin, Edward J.

    (The laryngoscope ; volume 126, supplement 1 (January 2016))

    2016  

    Author's details Ian N. Jacobs, MD; Robert A. Redden, BSE, MSE; Rachel Goldberg, BA; Michael Hast, BSE; Rebecca Salowe, BSE; Robert L. Mauck, PhD; Edward J. Doolin, MD
    Series title The laryngoscope ; volume 126, supplement 1 (January 2016)
    Collection
    Language English
    Size S21 Seiten, Illustrationen
    Publisher Wiley
    Publishing place Hoboken, NJ
    Publishing country United States
    Document type Book
    HBZ-ID HT018988506
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    Ul II Zs 82 -126,Suppl.1- verfügbar in Königswinter Königswinter

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  6. Article ; Online: Suspension bath bioprinting and maturation of anisotropic meniscal constructs.

    Prendergast, Margaret E / Heo, Su-Jin / Mauck, Robert L / Burdick, Jason A

    Biofabrication

    2023  Volume 15, Issue 3

    Abstract: Due to limited intrinsic healing capacity of the meniscus, meniscal injuries pose a significant clinical challenge. The most common method for treatment of damaged meniscal tissues, meniscectomy, leads to improper loading within the knee joint, which can ...

    Abstract Due to limited intrinsic healing capacity of the meniscus, meniscal injuries pose a significant clinical challenge. The most common method for treatment of damaged meniscal tissues, meniscectomy, leads to improper loading within the knee joint, which can increase the risk of osteoarthritis. Thus, there is a clinical need for the development of constructs for meniscal repair that better replicate meniscal tissue organization to improve load distributions and function over time. Advanced three-dimensional bioprinting technologies such as suspension bath bioprinting provide some key advantages, such as the ability to support the fabrication of complex structures using non-viscous bioinks. In this work, the suspension bath printing process is utilized to print anisotropic constructs with a unique bioink that contains embedded hydrogel fibers that align via shear stresses during printing. Constructs with and without fibers are printed and then cultured for up to 56 d
    MeSH term(s) Bioprinting/methods ; Hydrogels/chemistry ; Meniscus ; Technology
    Chemical Substances Hydrogels
    Language English
    Publishing date 2023-04-12
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2500944-8
    ISSN 1758-5090 ; 1758-5082
    ISSN (online) 1758-5090
    ISSN 1758-5082
    DOI 10.1088/1758-5090/acc3c3
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  7. Article ; Online: Multiplexed tape-stabilized cryohistology of mineralized large animal specimens.

    Zlotnick, Hannah M / Jiang, Xi / Mauck, Robert L / Dyment, Nathaniel A

    Journal of biological methods

    2022  Volume 9, Issue 4, Page(s) e166

    Abstract: Tape-stabilized cryohistology is a powerful histological method to reinforce tissue samples during and after sectioning, enhancing the overall image quality. This technique has widely been applied to section mineralized small animal ( ...

    Abstract Tape-stabilized cryohistology is a powerful histological method to reinforce tissue samples during and after sectioning, enhancing the overall image quality. This technique has widely been applied to section mineralized small animal (
    Language English
    Publishing date 2022-11-17
    Publishing country United States
    Document type Journal Article
    ISSN 2326-9901
    ISSN (online) 2326-9901
    DOI 10.14440/jbm.2022.389
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  8. Article ; Online: Developmental morphogens direct human induced pluripotent stem cells toward an annulus fibrosus-like cell phenotype.

    Peredo, Ana P / Tsinman, Tonia K / Bonnevie, Edward D / Jiang, Xi / Smith, Harvey E / Gullbrand, Sarah E / Dyment, Nathaniel A / Mauck, Robert L

    JOR spine

    2024  Volume 7, Issue 1, Page(s) e1313

    Abstract: Introduction: Therapeutic interventions for intervertebral disc herniation remain scarce due to the inability of endogenous annulus fibrosus (AF) cells to respond to injury and drive tissue regeneration. Unlike other orthopedic tissues, such as ... ...

    Abstract Introduction: Therapeutic interventions for intervertebral disc herniation remain scarce due to the inability of endogenous annulus fibrosus (AF) cells to respond to injury and drive tissue regeneration. Unlike other orthopedic tissues, such as cartilage, delivery of exogenous cells to the site of annular injury remains underdeveloped, largely due to a lack of an ideal cell source and the invasive nature of cell isolation. Human induced pluripotent stem cells (iPSCs) can be differentiated to specific cell fates using biochemical factors and are, therefore, an invaluable tool for cell therapy approaches. While differentiation protocols have been developed for cartilage and fibrous connective tissues (e.g., tendon), the signals that regulate the induction and differentiation of human iPSCs toward the AF fate remain unknown.
    Methods: iPSC-derived sclerotome cells were treated with various combinations of developmental signals including transforming growth factor beta 3 (TGF-β3), connective tissue growth factor (CTGF), platelet derived growth factor BB (PDGF-BB), insulin-like growth factor 1 (IGF-1), or the Hedgehog pathway activator, Purmorphamine, and gene expression changes in major AF-associated ECM genes were assessed. The top performing combination treatments were further validated by using three distinct iPSC lines and by assessing the production of upregulated ECM proteins of interest. To conduct a broader analysis of the transcriptomic shifts elicited by each factor combination, and to compare genetic profiles of treated cells to mature human AF cells, a 96.96 Fluidigm gene expression array was applied, and principal component analysis was employed to identify the transcriptional signatures of each cell population and treatment group in comparison to native AF cells.
    Results: TGF-β3, in combination with PDGF-BB, CTGF, or IGF-1, induced an upregulation of key AF ECM genes in iPSC-derived sclerotome cells. In particular, treatment with a combination of TGF-β3 with PDGF-BB for 14 days significantly increased gene expression of collagen II and aggrecan and increased protein deposition of collagen I and elastin compared to other treatment groups. Assessment of genes uniquely highly expressed by AF cells or SCL cells, respectively, revealed a shift toward the genetic profile of AF cells with the addition of TGF-β3 and PDGF-BB for 14 days.
    Discussion: These findings represent an initial approach to guide human induced pluripotent stem cells toward an AF-like fate for cellular delivery strategies.
    Language English
    Publishing date 2024-01-25
    Publishing country United States
    Document type Journal Article
    ISSN 2572-1143
    ISSN (online) 2572-1143
    DOI 10.1002/jsp2.1313
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  9. Article ; Online: Transient inhibition of meniscus cell migration following acute inflammatory challenge.

    Lemmon, Elisabeth A / Bonnevie, Edward D / Patel, Jay M / Miller, Liane M / Mauck, Robert L

    Journal of orthopaedic research : official publication of the Orthopaedic Research Society

    2023  Volume 41, Issue 9, Page(s) 2055–2064

    Abstract: Meniscus tears represent a common orthopedic injury that often requires surgery to restore pain-free function. The need for surgical intervention is due, in part, to the inflammatory and catabolic environment that inhibits meniscus healing after injury. ... ...

    Abstract Meniscus tears represent a common orthopedic injury that often requires surgery to restore pain-free function. The need for surgical intervention is due, in part, to the inflammatory and catabolic environment that inhibits meniscus healing after injury. In other organ systems, healing is dependent on the migration of cells to the site of injury; however, in the meniscus, it is currently unknown how the microenvironment dictates cell migration in the postinjury inflamed setting. Here, we investigated how inflammatory cytokines alter meniscal fibrochondrocyte (MFC) migration and sensation of microenvironmental stiffness. We further tested whether an FDA approved interleukin-1 receptor antagonist (IL-1Ra; Anakinra) could rescue migratory deficits caused by inflammatory challenge. When cultured in the presence of inflammatory cytokines (tumor necrosis factor-α [TNF-α] or interleukin-1β [IL-1β]) for 1 day, MFC migration was inhibited for 3 days before returning to control levels at Day 7. This migratory deficit was clear in three-dimensional as well, where fewer MFCs exposed to inflammatory cytokines migrated from a living meniscal explant compared with control. Notably, addition of IL-1Ra to MFCs previously exposed to IL-1β restored migration to baseline levels. This study demonstrates that joint inflammation can have negative impacts on meniscus cell migration and mechanosensation, affecting their potential for repair, and that resolution of this inflammation with concurrent anti-inflammatories can reverse these deficits. Future work will apply these findings to mitigate the negative consequences of joint inflammation and promote repair in a clinically relevant meniscus injury model.
    MeSH term(s) Humans ; Interleukin 1 Receptor Antagonist Protein/pharmacology ; Interleukin 1 Receptor Antagonist Protein/therapeutic use ; Meniscus ; Cytokines ; Tumor Necrosis Factor-alpha/metabolism ; Cell Movement ; Inflammation
    Chemical Substances Interleukin 1 Receptor Antagonist Protein ; Cytokines ; Tumor Necrosis Factor-alpha
    Language English
    Publishing date 2023-03-22
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 605542-4
    ISSN 1554-527X ; 0736-0266
    ISSN (online) 1554-527X
    ISSN 0736-0266
    DOI 10.1002/jor.25545
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    Zs.A 1879: Show issues Location:
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  10. Article ; Online: Physiology and Engineering of the Graded Interfaces of Musculoskeletal Junctions.

    Bonnevie, Edward D / Mauck, Robert L

    Annual review of biomedical engineering

    2018  Volume 20, Page(s) 403–429

    Abstract: The connective tissues of the musculoskeletal system can be grouped into fibrous, cartilaginous, and calcified tissues. While each tissue type has a distinct composition and function, the intersections between these tissues result in the formation of ... ...

    Abstract The connective tissues of the musculoskeletal system can be grouped into fibrous, cartilaginous, and calcified tissues. While each tissue type has a distinct composition and function, the intersections between these tissues result in the formation of complex, composite, and graded junctions. The complexity of these interfaces is a critical aspect of their healthy function, but poses a significant challenge for their repair. In this review, we describe the organization and structure of complex musculoskeletal interfaces, identify emerging technologies for engineering such structures, and outline the requirements for assessing the complex nature of these tissues in the context of recapitulating their function through tissue engineering.
    MeSH term(s) Animals ; Bone and Bones/physiology ; Cartilage, Articular/physiology ; Humans ; Intervertebral Disc/pathology ; Ligaments/pathology ; Temporomandibular Joint/pathology ; Tendons/pathology ; Tissue Engineering/methods ; Tissue Scaffolds
    Language English
    Publishing date 2018-04-11
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
    ZDB-ID 1448425-0
    ISSN 1545-4274 ; 1523-9829
    ISSN (online) 1545-4274
    ISSN 1523-9829
    DOI 10.1146/annurev-bioeng-062117-121113
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