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  1. Article ; Online: GULP1 deficiency reduces adipogenesis and glucose uptake via downregulation of PPAR signaling and disturbing of insulin/ERK signaling in 3T3-L1 cells.

    Kim, Soon-Young / Park, Seung-Yoon / Kim, Jung-Eun

    Journal of cellular physiology

    2024  Volume 239, Issue 2, Page(s) e31173

    Abstract: Obesity and metabolic disorders caused by alterations in lipid metabolism are major health issues in developed, affluent societies. Adipose tissue is the only organ that stores lipids and prevents lipotoxicity in other organs. Mature adipocytes can ... ...

    Abstract Obesity and metabolic disorders caused by alterations in lipid metabolism are major health issues in developed, affluent societies. Adipose tissue is the only organ that stores lipids and prevents lipotoxicity in other organs. Mature adipocytes can affect themselves and distant metabolism-related tissues by producing various adipokines, including adiponectin and leptin. The engulfment adaptor phosphotyrosine-binding domain-containing 1 (GULP1) regulates intracellular trafficking of glycosphingolipids and cholesterol, suggesting its close association with lipid metabolism. However, the role of GULP1 in adipocytes remains unknown. Therefore, this study aimed to investigate the function of GULP1 in adipogenesis, glucose uptake, and the insulin signaling pathway in adipocytes. A 3T3-L1 cell line with Gulp1 knockdown (shGulp1) and a 3T3-L1 control group (U6) were established. Changes in shGulp1 cells due to GULP1 deficiency were examined and compared to those in U6 cells using microarray analysis. Glucose uptake was monitored via insulin stimulation in shGulp1 and U6 cells using a 2-NBDG glucose uptake assay, and the insulin signaling pathway was investigated by western blot analysis. Adipogenesis was significantly delayed, lipid metabolism was altered, and several adipogenesis-related genes were downregulated in shGulp1 cells compared to those in U6 cells. Microarray analysis revealed significant inhibition of peroxisome proliferator-activated receptor signaling in shGulp1 cells compared with U6 cells. The production and secretion of adiponectin as well as the expression of adiponectin receptor were decreased in shGulp1 cells. In particular, compared with U6 cells, glucose uptake via insulin stimulation was significantly decreased in shGulp1 cells through the disturbance of ERK1/2 phosphorylation. This is the first study to identify the role of GULP1 in adipogenesis and insulin-stimulated glucose uptake by adipocytes, thereby providing new insights into the differentiation and functions of adipocytes and the metabolism of lipids and glucose, which can help better understand metabolic diseases.
    MeSH term(s) Animals ; Mice ; 3T3-L1 Cells ; Adipogenesis/genetics ; Adiponectin/genetics ; Adiponectin/metabolism ; Cell Differentiation ; Down-Regulation ; Glucose/metabolism ; Insulin/metabolism ; Lipids ; Peroxisome Proliferator-Activated Receptors/genetics ; Peroxisome Proliferator-Activated Receptors/metabolism ; PPAR gamma/metabolism ; Signal Transduction ; Extracellular Signal-Regulated MAP Kinases/metabolism
    Chemical Substances Adiponectin ; Glucose (IY9XDZ35W2) ; Insulin ; Lipids ; Peroxisome Proliferator-Activated Receptors ; PPAR gamma ; GULP protein, mouse ; Extracellular Signal-Regulated MAP Kinases (EC 2.7.11.24)
    Language English
    Publishing date 2024-01-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3116-1
    ISSN 1097-4652 ; 0021-9541
    ISSN (online) 1097-4652
    ISSN 0021-9541
    DOI 10.1002/jcp.31173
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  2. Article ; Online: Gulp1 regulates chondrocyte growth arrest and differentiation via the TGF-β/SMAD2/3 pathway.

    Kim, Dough / Kim, Jung-Eun / Lee, Seon Bhin / Lee, Na Yeon / Park, Seung-Yoon

    FEBS letters

    2024  Volume 598, Issue 8, Page(s) 935–944

    Abstract: Chondrocyte differentiation is crucial for cartilage formation. However, the complex processes and mechanisms coordinating chondrocyte proliferation and differentiation remain incompletely understood. Here, we report a novel function of the adaptor ... ...

    Abstract Chondrocyte differentiation is crucial for cartilage formation. However, the complex processes and mechanisms coordinating chondrocyte proliferation and differentiation remain incompletely understood. Here, we report a novel function of the adaptor protein Gulp1 in chondrocyte differentiation. Gulp1 expression is upregulated during chondrogenic differentiation. Gulp1 knockdown in chondrogenic ATDC5 cells reduces the expression of chondrogenic and hypertrophic marker genes during differentiation. Furthermore, Gulp1 knockdown impairs cell growth arrest during chondrocyte differentiation and reduces the expression of the cyclin-dependent kinase inhibitor p21. The activation of the TGF-β/SMAD2/3 pathway, which is associated with p21 expression in chondrocytes, is impaired in Gulp1 knockdown cells. Collectively, these results demonstrate that Gulp1 contributes to cell growth arrest and chondrocyte differentiation by modulating the TGF-β/SMAD2/3 pathway.
    MeSH term(s) Chondrocytes/metabolism ; Chondrocytes/cytology ; Smad2 Protein/metabolism ; Smad2 Protein/genetics ; Cell Differentiation ; Smad3 Protein/metabolism ; Smad3 Protein/genetics ; Animals ; Mice ; Signal Transduction ; Transforming Growth Factor beta/metabolism ; Chondrogenesis/genetics ; Cyclin-Dependent Kinase Inhibitor p21/metabolism ; Cyclin-Dependent Kinase Inhibitor p21/genetics ; Cell Proliferation ; Cell Line ; Adaptor Proteins, Signal Transducing/metabolism ; Adaptor Proteins, Signal Transducing/genetics ; Gene Knockdown Techniques ; Cell Cycle Checkpoints/genetics
    Chemical Substances Smad2 Protein ; Smad3 Protein ; Transforming Growth Factor beta ; Smad2 protein, mouse ; Cyclin-Dependent Kinase Inhibitor p21 ; Smad3 protein, mouse ; Adaptor Proteins, Signal Transducing
    Language English
    Publishing date 2024-03-29
    Publishing country England
    Document type Research Support, Non-U.S. Gov't ; Journal Article ; Letter
    ZDB-ID 212746-5
    ISSN 1873-3468 ; 0014-5793
    ISSN (online) 1873-3468
    ISSN 0014-5793
    DOI 10.1002/1873-3468.14862
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    In stock of ZB MED Cologne/Königswinter

    Zs.B 282: Show issues Location:
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  3. Article ; Online: Reply to the Letter to the Editor by Xu G.

    Park, Hyung Joo / Rim, Gongmin / Yoon, Seung Keun

    Journal of pediatric surgery

    2024  

    Language English
    Publishing date 2024-03-05
    Publishing country United States
    Document type Letter
    ZDB-ID 80165-3
    ISSN 1531-5037 ; 0022-3468
    ISSN (online) 1531-5037
    ISSN 0022-3468
    DOI 10.1016/j.jpedsurg.2024.02.035
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 607: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  4. Article ; Online: Productivity of Fish and Crop Growth and Characteristics of Bacterial Communities in the FLOCponics System

    Hwang, Ju-Ae / Park, Jun Seong / Jeong, Hae Seung / Kim, Hyeongsu / Oh, Seung-Yoon

    Fishes. 2023 Aug. 18, v. 8, no. 8

    2023  

    Abstract: Aquaponics (AP) and biofloc technology (BFT) systems rely heavily on bacterial communities to break down organic matter and cycle nutrients that are essential for fish and plant growth. The functional roles of bacterial communities in aquaculture systems ...

    Abstract Aquaponics (AP) and biofloc technology (BFT) systems rely heavily on bacterial communities to break down organic matter and cycle nutrients that are essential for fish and plant growth. The functional roles of bacterial communities in aquaculture systems are critical to their sustainable operation. Currently, the research on the combination of BFT and AP systems called FLOCponics (FP) is lacking, thereby hindering our ability to optimize their performance. Here, several characteristics (productivity of fish and crops, physicochemical properties of water, and bacterial community) in FP systems cultivating Japanese eel (Anguilla japonica) and leaf lettuce Caipira (Lactuca sativa) were compared to those in the BFT system. Additionally, the effect of fish density on the FP system was investigated. The results indicated that the FP system was more productive than the BFT system. Fish growth rate was highest in the FP system (52.6%), and the average body weight of eels was 168.2 ± 26.8 g in the FP system compared to 140.3 ± 27.0 g in the control (BFT, 5 kg/m²). However, increasing fish density resulted in lower growth rates, with a growth rate of 20.6% observed in the high-density (20 kg/m²) experimental group. The bacterial composition was also significantly different between the systems and fish densities, suggesting that bacterial communities may be closely related to the performance of the aquaponics system.
    Keywords Anguilla japonica ; Lactuca sativa ; animal growth ; aquaponics ; bacterial communities ; biofloc technology ; body weight ; fish ; leaf lettuce ; organic matter ; plant growth
    Language English
    Dates of publication 2023-0818
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ISSN 2410-3888
    DOI 10.3390/fishes8080422
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: The Role of Regulatory Focus and Emotion Recognition Bias in Cross-Cultural Negotiation

    Donghee Han / Hyewon Park / Seung-Yoon Rhee

    Sustainability, Vol 13, Iss 5, p

    2021  Volume 2659

    Abstract: Prior research on cross-cultural negotiation has emphasized the cognitive and the behavioral elements. This study takes a different perspective and presents a motivation–emotion model of cross-cultural negotiation. We propose that the cultural ... ...

    Abstract Prior research on cross-cultural negotiation has emphasized the cognitive and the behavioral elements. This study takes a different perspective and presents a motivation–emotion model of cross-cultural negotiation. We propose that the cultural differences in chronic regulatory focus will lead to cultural biases in emotion recognition, which in turn will affect negotiation behaviors. People are inclined to perceive and behave in ways that enhance regulatory fit. Westerners and East Asians, who each have different chronic regulatory focus, are likely to interpret the negotiation situation differently in order to increase their regulatory fit. Specifically, this study proposes that when the emotion of the opponent is ambiguous, people from different cultural backgrounds may show cultural biases in emotion recognition, concentrating on the emotion that fits their chronic regulatory focus. Drawing on the Emotion as Social Information (EASI) model, this study discusses how these cultural biases in emotion recognition can affect people’s negotiation behaviors. Finally, some possible moderators of the motivation–emotion model including power and emotion recognition accuracy are suggested to promote sustainable practices in cross-cultural negotiation.
    Keywords emotion recognition ; regulatory focus ; cross-cultural negotiation ; Environmental effects of industries and plants ; TD194-195 ; Renewable energy sources ; TJ807-830 ; Environmental sciences ; GE1-350
    Subject code 303
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Stabilin Receptors: Role as Phosphatidylserine Receptors.

    Park, Seung-Yoon / Kim, In-San

    Biomolecules

    2019  Volume 9, Issue 8

    Abstract: Phosphatidylserine is a membrane phospholipid that is localized to the inner leaflet of the plasma membrane. Phosphatidylserine externalization to the outer leaflet of the plasma membrane is an important signal for various physiological processes, ... ...

    Abstract Phosphatidylserine is a membrane phospholipid that is localized to the inner leaflet of the plasma membrane. Phosphatidylserine externalization to the outer leaflet of the plasma membrane is an important signal for various physiological processes, including apoptosis, platelet activation, cell fusion, lymphocyte activation, and regenerative axonal fusion. Stabilin-1 and stabilin-2 are membrane receptors that recognize phosphatidylserine on the cell surface. Here, we discuss the functions of Stabilin-1 and stabilin-2 as phosphatidylserine receptors in apoptotic cell clearance (efferocytosis) and cell fusion, and their ligand-recognition and signaling pathways.
    MeSH term(s) Animals ; Cell Adhesion Molecules, Neuronal/metabolism ; Humans
    Chemical Substances Cell Adhesion Molecules, Neuronal
    Language English
    Publishing date 2019-08-20
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2701262-1
    ISSN 2218-273X ; 2218-273X
    ISSN (online) 2218-273X
    ISSN 2218-273X
    DOI 10.3390/biom9080387
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  7. Article: Acute Toxicity Evaluation of the Disinfectant Containing Percarbonate and Tetraacetylethylenediamine by Measuring Behavioral Responses of Small Fish Using Image Analysis.

    Seo, Seung-Yoon / Park, Yeon-Ho / Jung, Sang-Kyu / Kim, Jinku

    Biotechnology and bioprocess engineering : BBE

    2022  Volume 27, Issue 4, Page(s) 687–696

    Abstract: Disinfectants containing percarbonate and tetraacetylethylenediamine (TAED) has been developed as an effective and relatively safe disinfectant to destroy viruses and bacteria in animals and humans, however it is known that most disinfectants can cause ... ...

    Abstract Disinfectants containing percarbonate and tetraacetylethylenediamine (TAED) has been developed as an effective and relatively safe disinfectant to destroy viruses and bacteria in animals and humans, however it is known that most disinfectants can cause danger to living organisms including humans. In the current study, acute toxicity of the disinfectant composed of percarbonate and TAED was assessed by measuring behavioral responses as well as lethal concentrations of aquatic organisms such as medaka and zebrafish when they were exposed to it. First, the breeding water properties were determined by measuring dissolved oxygen (DO) and pH changes over time up to 96 h in acute toxicity tests using the medaka, and the lethal concentration 50% (LC50, 88.39 ppm) was calculated using the lethality rate of the fish. This experiment was conducted in compliance with traditional OECD guidelines. Second, the assessment of behavioral responses (locomotive activity and swimming speed) with the zebrafish were assessed by the image analysis to capture the images per second for three hours, and the collected data were processed using image analysis to calculate the locomotive activity and swimming speed. Finally, the LC50 (135.76 ppm) of the disinfectant to the fish was also measured after three hours. Overall, the data revealed that LC50 of the disinfectant may be affected by the pH of the water exposed to the disinfectant, not by the DO in the water. In addition, the results from the image analysis indicated that the behavioral responses of the fish can further assess the acute toxicity of the disinfectant at concentrations below the LC50 and there was a relationship (R
    Electronic supplementary material esm: The online version of this article (doi: 10.1007/s12257-021-0419-0) contains supplementary material, which is available to authorized users.
    Language English
    Publishing date 2022-06-12
    Publishing country Korea (South)
    Document type Journal Article
    ZDB-ID 2125481-3
    ISSN 1976-3816 ; 1226-8372
    ISSN (online) 1976-3816
    ISSN 1226-8372
    DOI 10.1007/s12257-021-0419-0
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  8. Article ; Online: Harnessing immune checkpoints in myeloid lineage cells for cancer immunotherapy.

    Park, Seung-Yoon / Kim, In-San

    Cancer letters

    2019  Volume 452, Page(s) 51–58

    Abstract: Myeloid lineage immune cells, such as macrophages and dendritic cells, play important roles in the induction of antitumor immunity during the initial stage of the cancer-immunity cycle, eliciting antitumor adaptive immunity by phagocytosing cancer cells ... ...

    Abstract Myeloid lineage immune cells, such as macrophages and dendritic cells, play important roles in the induction of antitumor immunity during the initial stage of the cancer-immunity cycle, eliciting antitumor adaptive immunity by phagocytosing cancer cells and processing cancer-specific antigens, and then presenting these antigens to T cells. During this process, cancer cell phagocytosis can be prevented by inhibitory signals, and the signaling cascades that elicit immune responses against cancer antigens can be inhibited by immunosuppressive myeloid cells in the tumor microenvironment. A number of therapeutic strategies for enhancing cancer cell phagocytosis and promoting antitumor immunity by targeting myeloid lineage cells have recently been developed. Here, we discuss recent advances in cancer immunotherapy that involve the targeting of myeloid lineage immune cells to induce effective antitumor immunity.
    MeSH term(s) Animals ; Antineoplastic Agents, Immunological/adverse effects ; Antineoplastic Agents, Immunological/therapeutic use ; Cell Lineage ; Humans ; Immunotherapy/adverse effects ; Immunotherapy/methods ; Immunotherapy, Adoptive/adverse effects ; Molecular Targeted Therapy ; Myeloid Cells/drug effects ; Myeloid Cells/immunology ; Myeloid Cells/metabolism ; Myeloid Cells/pathology ; Neoplasms/immunology ; Neoplasms/metabolism ; Neoplasms/pathology ; Neoplasms/therapy ; Phagocytosis/drug effects ; Signal Transduction ; Treatment Outcome ; Tumor Escape ; Tumor Microenvironment
    Chemical Substances Antineoplastic Agents, Immunological
    Language English
    Publishing date 2019-03-22
    Publishing country Ireland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 195674-7
    ISSN 1872-7980 ; 0304-3835
    ISSN (online) 1872-7980
    ISSN 0304-3835
    DOI 10.1016/j.canlet.2019.03.018
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    Zs.A 1177: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  9. Article ; Online: Chorea Associated with Genetic-Confirmed CADASIL.

    Kim, Min Seung / Park, Don Gueu / Yoon, Jung Han

    Movement disorders clinical practice

    2023  Volume 10, Issue 9, Page(s) 1433–1435

    Language English
    Publishing date 2023-08-08
    Publishing country United States
    Document type Journal Article
    ISSN 2330-1619
    ISSN (online) 2330-1619
    DOI 10.1002/mdc3.13847
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  10. Article: The Influence of Microfungi on the Mycelial Growth of Ectomycorrhizal Fungus

    Oh, Seung-Yoon / Park, Myung Soo / Lim, Young Woon

    Microorganisms

    2019  Volume 7, Issue 6

    Abstract: Pine mushroom ( ...

    Abstract Pine mushroom (
    Language English
    Publishing date 2019-06-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms7060169
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