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  1. Article ; Online: A desthiobiotin labelled NAD

    Rieth, Sonja / Spliesgar, Daniel / Orth, Jan / Lehner, Maike / Kasprzyk, Renata / Stengel, Florian / Marx, Andreas

    Chembiochem : a European journal of chemical biology

    2024  Volume 25, Issue 5, Page(s) e202300797

    Abstract: ADP-ribosylation is a post-translational modification catalyzed by the enzyme family of polyadenosine diphosphate (ADP)-ribose) polymerases (PARPs). This enzymatic process involves the transfer of single or multiple ADP-ribose molecules onto proteins, ... ...

    Abstract ADP-ribosylation is a post-translational modification catalyzed by the enzyme family of polyadenosine diphosphate (ADP)-ribose) polymerases (PARPs). This enzymatic process involves the transfer of single or multiple ADP-ribose molecules onto proteins, utilizing nicotinamide adenine dinucleotide (NAD
    MeSH term(s) NAD ; Poly(ADP-ribose) Polymerase Inhibitors ; Binding Sites ; Niacinamide/pharmacology ; Biotin/analogs & derivatives
    Chemical Substances desthiobiotin (71U5JB52KS) ; NAD (0U46U6E8UK) ; Poly(ADP-ribose) Polymerase Inhibitors ; Niacinamide (25X51I8RD4) ; Biotin (6SO6U10H04)
    Language English
    Publishing date 2024-01-31
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2020469-3
    ISSN 1439-7633 ; 1439-4227
    ISSN (online) 1439-7633
    ISSN 1439-4227
    DOI 10.1002/cbic.202300797
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  2. Article ; Online: Enzymatic Assays to Explore Viral mRNA Capping Machinery.

    Kasprzyk, Renata / Jemielity, Jacek

    Chembiochem : a European journal of chemical biology

    2021  Volume 22, Issue 23, Page(s) 3236–3253

    Abstract: In eukaryotes, mRNA is modified by the addition of the 7-methylguanosine ( ... ...

    Abstract In eukaryotes, mRNA is modified by the addition of the 7-methylguanosine (m
    MeSH term(s) COVID-19/virology ; Enzyme Assays ; RNA Caps ; RNA, Messenger/genetics ; RNA, Viral/genetics ; SARS-CoV-2/genetics
    Chemical Substances RNA Caps ; RNA, Messenger ; RNA, Viral
    Language English
    Publishing date 2021-08-03
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2020469-3
    ISSN 1439-7633 ; 1439-4227
    ISSN (online) 1439-7633
    ISSN 1439-4227
    DOI 10.1002/cbic.202100291
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  3. Article ; Online: Assessment of Oral Health in Long-Term Enteral and Parenteral Nutrition Patients: Significant Aspects of Nursing Care.

    Terech-Skóra, Sylwia / Kasprzyk-Mazur, Joanna / Leyk-Kolańczak, Magdalena / Kruk, Agnieszka / Piotrkowska, Renata / Mędrzycka-Dąbrowska, Wioletta / Książek, Janina

    International journal of environmental research and public health

    2023  Volume 20, Issue 4

    Abstract: Oral health is an underestimated factor affecting overall human health and quality of life. Long-term enteral or parenteral nutritional treatment requires not only regular assessment of access routes, the patient's nutritional status, and tolerance to ... ...

    Abstract Oral health is an underestimated factor affecting overall human health and quality of life. Long-term enteral or parenteral nutritional treatment requires not only regular assessment of access routes, the patient's nutritional status, and tolerance to the selected method of nutrition but also of oral health. This article discusses the connections between the influence of chewing function, salivation, and xerostomia on the health of the oral cavity of patients on long-term enteral and parenteral nutrition. In addition, the role of nurses in assessing oral health is presented as well as crucial elements of a comprehensive oral assessment in a nursing care plan. Patients receiving long-term enteral and parenteral nutrition have an increased risk of developing oral diseases. Increasing knowledge about the factors affecting oral health among nurses is crucial to provide appropriate care for patients requiring long-term nutritional treatment with omission of the natural route of food intake. Regular assessment of oral health by nurses should be an important aspect in long-term nutritional treatment recommendations.
    MeSH term(s) Humans ; Oral Health ; Quality of Life ; Enteral Nutrition/methods ; Parenteral Nutrition ; Nursing Care ; Nutrition Assessment
    Language English
    Publishing date 2023-02-15
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2175195-X
    ISSN 1660-4601 ; 1661-7827
    ISSN (online) 1660-4601
    ISSN 1661-7827
    DOI 10.3390/ijerph20043381
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  4. Article: Karyotype Abnormalities in the X Chromosome Predict Response to the Growth Hormone Therapy in Turner Syndrome.

    Kasprzyk, Jakub / Włodarczyk, Marcin / Sobolewska-Włodarczyk, Aleksandra / Wieczorek-Szukała, Katarzyna / Stawerska, Renata / Hilczer, Maciej / Lewiński, Andrzej

    Journal of clinical medicine

    2021  Volume 10, Issue 21

    Abstract: Short stature is characteristic for Turner syndrome (TS) patients, and particular karyotype abnormalities of the X chromosome may be associated with different responsiveness to recombinant human GH (rhGH) therapy. The aim of the study was to analyze the ... ...

    Abstract Short stature is characteristic for Turner syndrome (TS) patients, and particular karyotype abnormalities of the X chromosome may be associated with different responsiveness to recombinant human GH (rhGH) therapy. The aim of the study was to analyze the effect of different types of TS karyotype abnormalities on the response to rhGH therapy. A total of 57 prepubertal patients with TS treated with rhGH with a 3 year follow-up were enrolled in the study and categorized according to their karyotype as X monosomy (
    Language English
    Publishing date 2021-10-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm10215076
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  5. Article ; Online: Ebselen derivatives inhibit SARS-CoV-2 replication by inhibition of its essential proteins: PL

    Zmudzinski, Mikolaj / Rut, Wioletta / Olech, Kamila / Granda, Jarosław / Giurg, Mirosław / Burda-Grabowska, Małgorzata / Kaleta, Rafał / Zgarbova, Michala / Kasprzyk, Renata / Zhang, Linlin / Sun, Xinyuanyuan / Lv, Zongyang / Nayak, Digant / Kesik-Brodacka, Malgorzata / Olsen, Shaun K / Weber, Jan / Hilgenfeld, Rolf / Jemielity, Jacek / Drag, Marcin

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 9161

    Abstract: Proteases encoded by SARS-CoV-2 constitute a promising target for new therapies against COVID-19. SARS-CoV-2 main protease ( ... ...

    Abstract Proteases encoded by SARS-CoV-2 constitute a promising target for new therapies against COVID-19. SARS-CoV-2 main protease (M
    MeSH term(s) Humans ; SARS-CoV-2/metabolism ; COVID-19 ; Methyltransferases ; Peptide Hydrolases ; Antiviral Agents/pharmacology ; Antiviral Agents/metabolism ; Cysteine Endopeptidases/metabolism ; Protease Inhibitors/pharmacology ; Molecular Docking Simulation
    Chemical Substances ebselen (40X2P7DPGH) ; Methyltransferases (EC 2.1.1.-) ; Peptide Hydrolases (EC 3.4.-) ; Antiviral Agents ; Cysteine Endopeptidases (EC 3.4.22.-) ; Protease Inhibitors
    Language English
    Publishing date 2023-06-06
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-35907-w
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  6. Article ; Online: Evaluation of carboxyfluorescein-labeled 7-methylguanine nucleotides as probes for studying cap-binding proteins by fluorescence anisotropy.

    Wojtczak, Anna / Kasprzyk, Renata / Warmiński, Marcin / Ubych, Krystian / Kubacka, Dorota / Sikorski, Pawel J / Jemielity, Jacek / Kowalska, Joanna

    Scientific reports

    2021  Volume 11, Issue 1, Page(s) 7687

    Abstract: Fluorescence anisotropy (FA) is a powerful technique for the discovery of protein inhibitors in a high-throughput manner. In this study, we sought to develop new universal FA-based assays for the evaluation of compounds targeting mRNA 5' cap-binding ... ...

    Abstract Fluorescence anisotropy (FA) is a powerful technique for the discovery of protein inhibitors in a high-throughput manner. In this study, we sought to develop new universal FA-based assays for the evaluation of compounds targeting mRNA 5' cap-binding proteins of therapeutic interest, including eukaryotic translation initiation factor 4E and scavenger decapping enzyme. For this purpose, a library of 19 carboxyfluorescein probes based on 7-methylguanine nucleotides was evaluated as FA probes for these proteins. Optimal probe:protein systems were further investigated in competitive binding experiments and adapted for high-throughput screening. Using a small in-house library of compounds, we verified and confirmed the accuracy of the developed FA assay to study cap-binding protein binders. The applications of the most promising probes were then extended to include evaluation of allosteric inhibitors as well as RNA ligands. From this analysis, we confirmed the utility of the method to study small molecule ligands and evaluate differently 5' capped RNAs.
    MeSH term(s) Fluoresceins/chemistry ; Fluorescence Polarization/methods ; Guanine/analogs & derivatives ; Guanine/chemistry ; Molecular Probes/chemistry
    Chemical Substances Fluoresceins ; Molecular Probes ; 6-carboxyfluorescein (3301-79-9) ; Guanine (5Z93L87A1R) ; 7-methylguanine (661J4K04NB)
    Language English
    Publishing date 2021-04-08
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-021-87306-8
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  7. Article ; Online: Fluorescence-Based Activity Screening Assay Reveals Small Molecule Inhibitors of Vaccinia Virus mRNA Decapping Enzyme D9.

    Bednarczyk, Marcelina / Peters, Jessica K / Kasprzyk, Renata / Starek, Jagoda / Warminski, Marcin / Spiewla, Tomasz / Mugridge, Jeffrey S / Gross, John D / Jemielity, Jacek / Kowalska, Joanna

    ACS chemical biology

    2022  Volume 17, Issue 6, Page(s) 1460–1471

    Abstract: Vaccinia virus (VACV) represents a family of poxviruses, which possess their own decapping machinery as a part of their strategy to eliminate host mRNAs and evade the innate immune response. D9 is one of the two encoded VACV decapping enzymes that is ... ...

    Abstract Vaccinia virus (VACV) represents a family of poxviruses, which possess their own decapping machinery as a part of their strategy to eliminate host mRNAs and evade the innate immune response. D9 is one of the two encoded VACV decapping enzymes that is responsible for cap removal from the 5' end of both host mRNA transcripts and viral double-stranded RNAs. Little is known about the structural requirements for D9 inhibition by small molecules. Here, we identified a minimal D9 substrate and used it to develop a real-time fluorescence assay for inhibitor discovery and characterization. We screened a panel of nucleotide-derived substrate analogues and pharmacologically active candidates to identify several compounds with nano- and low micromolar IC
    MeSH term(s) Endoribonucleases/metabolism ; Fluorescence ; Humans ; Nucleotides ; RNA Caps/metabolism ; RNA, Messenger/metabolism ; Vaccinia virus/genetics ; Viral Proteins/metabolism
    Chemical Substances Nucleotides ; RNA Caps ; RNA, Messenger ; Viral Proteins ; mRNA decapping enzymes ; Endoribonucleases (EC 3.1.-)
    Language English
    Publishing date 2022-05-16
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1554-8937
    ISSN (online) 1554-8937
    DOI 10.1021/acschembio.2c00049
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  8. Article ; Online: N1-Propargylguanosine Modified mRNA Cap Analogs: Synthesis, Reactivity, and Applications to the Study of Cap-Binding Proteins.

    Kopcial, Michal / Wojtczak, Blazej A / Kasprzyk, Renata / Kowalska, Joanna / Jemielity, Jacek

    Molecules (Basel, Switzerland)

    2019  Volume 24, Issue 10

    Abstract: The mRNA 5' cap consists of N7-methylguanosine bound by a 5',5'-triphosphate bridge to the first nucleotide of the transcript. The cap interacts with various specific proteins and participates in all key mRNA-related processes, which may be of ... ...

    Abstract The mRNA 5' cap consists of N7-methylguanosine bound by a 5',5'-triphosphate bridge to the first nucleotide of the transcript. The cap interacts with various specific proteins and participates in all key mRNA-related processes, which may be of therapeutic relevance. There is a growing demand for new biophysical and biochemical methods to study cap-protein interactions and identify the factors which inhibit them. The development of such methods can be aided by the use of properly designed fluorescent molecular probes. Herein, we synthesized a new class of m
    MeSH term(s) Click Chemistry ; Cycloaddition Reaction ; Guanosine/chemistry ; Humans ; RNA Cap Analogs/chemical synthesis ; RNA Cap Analogs/chemistry ; RNA Cap Analogs/metabolism ; RNA Cap-Binding Proteins/chemistry ; RNA Cap-Binding Proteins/metabolism
    Chemical Substances RNA Cap Analogs ; RNA Cap-Binding Proteins ; Guanosine (12133JR80S)
    Language English
    Publishing date 2019-05-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules24101899
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    Zs.MO 81: Show issues

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  9. Article ; Online: Fluorescent Turn-On Probes for the Development of Binding and Hydrolytic Activity Assays for mRNA Cap-Recognizing Proteins.

    Kasprzyk, Renata / Starek, Beata J / Ciechanowicz, Sylwia / Kubacka, Dorota / Kowalska, Joanna / Jemielity, Jacek

    Chemistry (Weinheim an der Bergstrasse, Germany)

    2019  Volume 25, Issue 27, Page(s) 6728–6740

    Abstract: ... The ... ...

    Abstract The m
    MeSH term(s) Animals ; Endoribonucleases/antagonists & inhibitors ; Endoribonucleases/genetics ; Endoribonucleases/metabolism ; Eukaryotic Initiation Factor-4E/chemistry ; Eukaryotic Initiation Factor-4E/genetics ; Eukaryotic Initiation Factor-4E/metabolism ; Fluorescent Dyes/chemical synthesis ; Fluorescent Dyes/chemistry ; Fluorescent Dyes/metabolism ; Guanosine Triphosphate/analogs & derivatives ; Guanosine Triphosphate/chemical synthesis ; Guanosine Triphosphate/metabolism ; HEK293 Cells ; HeLa Cells ; Humans ; Hydrolysis ; Inhibitory Concentration 50 ; Kinetics ; Ligands ; Mice ; Protein Binding ; Pyrenes/chemistry ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/chemistry ; Recombinant Proteins/isolation & purification ; Spectrometry, Fluorescence
    Chemical Substances Eukaryotic Initiation Factor-4E ; Fluorescent Dyes ; Ligands ; Pyrenes ; Recombinant Proteins ; Guanosine Triphosphate (86-01-1) ; pyrene (9E0T7WFW93) ; Endoribonucleases (EC 3.1.-) ; DcpS protein, mouse (EC 3.1.27.-)
    Language English
    Publishing date 2019-04-01
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1478547-x
    ISSN 1521-3765 ; 0947-6539
    ISSN (online) 1521-3765
    ISSN 0947-6539
    DOI 10.1002/chem.201900051
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  10. Article ; Online: 2'-O-Methylation of the second transcribed nucleotide within the mRNA 5' cap impacts the protein production level in a cell-specific manner and contributes to RNA immune evasion.

    Drazkowska, Karolina / Tomecki, Rafal / Warminski, Marcin / Baran, Natalia / Cysewski, Dominik / Depaix, Anaïs / Kasprzyk, Renata / Kowalska, Joanna / Jemielity, Jacek / Sikorski, Pawel J

    Nucleic acids research

    2022  Volume 50, Issue 16, Page(s) 9051–9071

    Abstract: In mammals, m7G-adjacent nucleotides undergo extensive modifications. Ribose of the first or first and second transcribed nucleotides can be subjected to 2'-O-methylation to form cap1 or cap2, respectively. When the first transcribed nucleotide is 2'-O- ... ...

    Abstract In mammals, m7G-adjacent nucleotides undergo extensive modifications. Ribose of the first or first and second transcribed nucleotides can be subjected to 2'-O-methylation to form cap1 or cap2, respectively. When the first transcribed nucleotide is 2'-O-methylated adenosine, it can be additionally modified to N6,2'-O-dimethyladenosine (m6Am). Recently, the crucial role of cap1 in distinguishing between 'self' and 'non-self' in mammalian cells during viral infection was revealed. Here, we attempted to understand the impact of cap methylations on RNA-related processes. Therefore, we synthesized tetranucleotide cap analogues and used them for RNA capping during in vitro transcription. Using this tool, we found that 2'-O-methylation of the second transcribed nucleotide within the mRNA 5' cap influences protein production levels in a cell-specific manner. This modification can strongly hamper protein biosynthesis or have no influence on protein production levels, depending on the cell line. Interestingly, 2'-O-methylation of the second transcribed nucleotide and the presence of m6Am as the first transcribed nucleotide serve as determinants that define transcripts as 'self' and contribute to transcript escape from the host innate immune response. Additionally, cap methylation status does not influence transcript affinity towards translation initiation factor eIF4E or in vitro susceptibility to decapping by DCP2; however, we observe the resistance of cap2-RNA to DXO (decapping exoribonuclease)-mediated decapping and degradation.
    MeSH term(s) Animals ; Methylation ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; RNA Caps/genetics ; RNA Caps/metabolism ; Nucleotides/metabolism ; Immune Evasion ; Mammals/genetics
    Chemical Substances RNA, Messenger ; RNA Caps ; Nucleotides
    Language English
    Publishing date 2022-08-26
    Publishing country England
    Document type Journal Article
    ZDB-ID 186809-3
    ISSN 1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
    ISSN (online) 1362-4962 ; 1362-4954
    ISSN 0301-5610 ; 0305-1048
    DOI 10.1093/nar/gkac722
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1067: Show issues Location:
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