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  1. Article: Epidemiology and antibiotic sensitivity of Staphylococcus aureus nasal carriage in children in Hungary.

    Laub, Krisztina / Tóthpál, Adrienn / Kardos, Szilvia / Dobay, Orsolya

    Acta microbiologica et immunologica Hungarica

    2017  Volume 64, Issue 1, Page(s) 51–62

    Abstract: The aim of this study was to assess the Staphylococcus aureus nasal carriage rate in healthy children all over Hungary and to specify some risk factors, the antibiotic resistance patterns of the bacteria, and their genetic relatedness. In total, 878 ... ...

    Abstract The aim of this study was to assess the Staphylococcus aureus nasal carriage rate in healthy children all over Hungary and to specify some risk factors, the antibiotic resistance patterns of the bacteria, and their genetic relatedness. In total, 878 children (aged 3-6 years) were screened at 21 day-care centers in 16 different cities in Hungary, between February 2009 and December 2011. Samples taken from both nostrils were cultured on blood agar, and suspected S. aureus isolates were identified by β-hemolysis, catalase positivity, clump test, and nucA PCR. Methicillin-resistant strains were screened by mecA and mecC PCR. Antibiotic susceptibility was determined by agar dilution or gradient test strips. Pulsed-field gel electrophoresis was used for genotyping. S. aureus carriage rate was found to be 21.3%, which correlates well with international data. We found no statistically significant correlation between the gender or the sibling status and S. aureus carriage. All isolates were sensitive to oxacillin, trimethoprim-sulfamethoxazole, and mupirocin. The resistance rates for erythromycin, ciprofloxacin, clindamycin, gentamicin, and tetracycline were 7.5%, 0.5%, 1.1%, 3.7%, and 4.3%, respectively. The isolates showed very high genetic diversity. In summary, carried S. aureus isolates are more sensitive to antibiotics compared with clinical isolates in Hungary, and methicillin-resistant S. aureus carriage rate is very low yet.
    MeSH term(s) Anti-Bacterial Agents/pharmacology ; Carrier State/microbiology ; Child ; Child, Preschool ; Drug Resistance, Bacterial ; Female ; Genotype ; Humans ; Hungary/epidemiology ; Male ; Microbial Sensitivity Tests ; Nose/microbiology ; Phylogeny ; Staphylococcal Infections/epidemiology ; Staphylococcal Infections/microbiology ; Staphylococcus aureus/classification ; Staphylococcus aureus/drug effects ; Staphylococcus aureus/genetics ; Staphylococcus aureus/isolation & purification
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2017-03-01
    Publishing country Hungary
    Document type Journal Article
    ZDB-ID 918256-1
    ISSN 1588-2640 ; 1217-8950
    ISSN (online) 1588-2640
    ISSN 1217-8950
    DOI 10.1556/030.64.2017.001
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  2. Article ; Online: High prevalence of group B streptococcus ST17 hypervirulent clone among non-pregnant patients from a Hungarian venereology clinic.

    Kardos, Szilvia / Tóthpál, Adrienn / Laub, Krisztina / Kristóf, Katalin / Ostorházi, Eszter / Rozgonyi, Ferenc / Dobay, Orsolya

    BMC infectious diseases

    2019  Volume 19, Issue 1, Page(s) 1009

    Abstract: Background: Although Streptococcus agalactiae is the leading causative agent of neonatal sepsis and meningitis, recently it is increasingly isolated from non-pregnant adults. The relation between its presence in the genitourinary tract and manifested ... ...

    Abstract Background: Although Streptococcus agalactiae is the leading causative agent of neonatal sepsis and meningitis, recently it is increasingly isolated from non-pregnant adults. The relation between its presence in the genitourinary tract and manifested clinical symptoms of STD patients remains an open question. In this study, a complex epidemiological investigation of GBS isolates from a venerology clinic was performed.
    Methods: Ninety-six GBS isolates were serotyped and their genetic relatedness determined by PFGE. MLST was also performed for a subset of 20 isolates. The antibiotic susceptibility was tested with agar dilution. Surface proteins and the ST-17 hypervirulent clone was detected by PCR.
    Results: The serotype prevalence was the following: V (29.2%), III (27.1%), Ia (22.9%), IV (10.4%), II (5.2%) and Ib (4.2%). A strong association was demonstrated between surface protein genes and serotypes. All isolates were fully susceptible to penicillin, but erythromycin and clindamycin resistance was high (41.7 and 35.4%, respectively), and 8 phenotypically macrolide sensitive isolates carried the ermB gene. 21.9% of all strains belonged to the hypervirulent ST17 clone, most being of serotype III and all were rib +. We found a few serotype IV isolates belonging to several STs and one serotype V/ST110 strain, containing a 44-bp deletion in the atr allele.
    Conclusions: The presence of silent ermB genes is of worry, as their expression upon macrolide exposure could lead to unforeseen therapeutic failure, while clindamycin is used for intrapartum antibiotic prophylaxis, in case of penicillin allergy. The other alarming result is the high prevalence of ST17 among these strains from STD patients, who could be sources of further infections. This is the first report from Hungary providing both serotyping and genotyping data of GBS isolates. These results could be helpful for vaccine production as the major vaccine candidates are capsular antigens or surface proteins.
    MeSH term(s) Adult ; Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/genetics ; DNA, Bacterial/genetics ; DNA, Bacterial/metabolism ; Drug Resistance, Bacterial/drug effects ; Female ; Humans ; Hungary/epidemiology ; Macrolides/pharmacology ; Microbial Sensitivity Tests ; Phenotype ; Prevalence ; Serogroup ; Streptococcal Infections/diagnosis ; Streptococcal Infections/epidemiology ; Streptococcus agalactiae/drug effects ; Streptococcus agalactiae/genetics ; Streptococcus agalactiae/isolation & purification ; Streptococcus agalactiae/pathogenicity ; Virulence/genetics
    Chemical Substances Anti-Bacterial Agents ; Bacterial Proteins ; DNA, Bacterial ; Macrolides
    Language English
    Publishing date 2019-11-28
    Publishing country England
    Document type Journal Article
    ISSN 1471-2334
    ISSN (online) 1471-2334
    DOI 10.1186/s12879-019-4626-7
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  3. Article: High prevalence of Staphylococcus aureus nasal carriage among children in Szolnok, Hungary.

    Laub, Krisztina / Tóthpál, Adrienn / Kovács, Eszter / Sahin-Tóth, Judit / Horváth, Andrea / Kardos, Szilvia / Dobay, Orsolya

    Acta microbiologica et immunologica Hungarica

    2018  Volume 65, Issue 1, Page(s) 59–72

    Abstract: We collected nasal samples from 1,390 healthy 3-7 years old children in Szolnok city, Hungary, in 2012. We detected 476 Staphylococcus aureus isolates from 474 children. In two occasions, two different S. aureus were isolated, based on hemolysis type and ...

    Abstract We collected nasal samples from 1,390 healthy 3-7 years old children in Szolnok city, Hungary, in 2012. We detected 476 Staphylococcus aureus isolates from 474 children. In two occasions, two different S. aureus were isolated, based on hemolysis type and pulsed-field gel electrophoresis pattern. S. aureus carriage rate was calculated to be 34.1% similar to others studies. Male gender was found to be a risk factor for carriage by statistical analysis. Altogether, four methicillin-resistant S. aureus (MRSA) strains were detected by mecA polymerase chain reaction, which means 0.8% community-acquired MRSA prevalence among the S. aureus isolates. All MRSA strains harbored the SCCmec type IV cassette (typical for CA-MRSA) and belonged to ST45 by multilocus sequence typing. During antibiotic susceptibility testing, we measured the following resistance rates: 0.0% for mupirocin, 0.2% for ciprofloxacin, 0.6% for gentamicin and oxacillin, 3.4% for tetracycline, 9.5% for clindamycin, 10.3% for erythromycin, and 91.4% for penicillin, which are generally lower compared with Hungarian clinical isolates.
    MeSH term(s) Anti-Bacterial Agents/pharmacology ; Carrier State/epidemiology ; Carrier State/microbiology ; Child ; Child, Preschool ; Drug Resistance, Bacterial ; Female ; Humans ; Hungary/epidemiology ; Male ; Microbial Sensitivity Tests ; Nose/microbiology ; Prevalence ; Staphylococcal Infections/epidemiology ; Staphylococcal Infections/microbiology ; Staphylococcus aureus/classification ; Staphylococcus aureus/drug effects ; Staphylococcus aureus/genetics ; Staphylococcus aureus/isolation & purification
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2018-03-01
    Publishing country Hungary
    Document type Journal Article
    ZDB-ID 918256-1
    ISSN 1588-2640 ; 1217-8950
    ISSN (online) 1588-2640
    ISSN 1217-8950
    DOI 10.1556/030.65.2018.001
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  4. Article: Bicarbonate Inhibits Bacterial Growth and Biofilm Formation of Prevalent Cystic Fibrosis Pathogens.

    Dobay, Orsolya / Laub, Krisztina / Stercz, Balázs / Kéri, Adrienn / Balázs, Bernadett / Tóthpál, Adrienn / Kardos, Szilvia / Jaikumpun, Pongsiri / Ruksakiet, Kasidid / Quinton, Paul M / Zsembery, Ákos

    Frontiers in microbiology

    2018  Volume 9, Page(s) 2245

    Abstract: We investigated the effects of bicarbonate on the growth of several different bacteria as well as its effects on biofilm formation and intracellular cAMP concentration ... ...

    Abstract We investigated the effects of bicarbonate on the growth of several different bacteria as well as its effects on biofilm formation and intracellular cAMP concentration in
    Language English
    Publishing date 2018-09-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2018.02245
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  5. Article: Detection of Staphylococcus aureus nasal carriage in healthy young adults from a Hungarian University.

    Laub, Krisztina / Kardos, Szilvia / Nagy, K / Dobay, Orsolya

    Acta microbiologica et immunologica Hungarica

    2011  Volume 58, Issue 1, Page(s) 75–84

    Abstract: Asymptomatic carriage of Staphylococcus aureus in healthy individuals has a high prevalence, especially in children and young adults. Nasal colonisation is a well-known risk factor for subsequent severe infection, or can be the source of transmission of ... ...

    Abstract Asymptomatic carriage of Staphylococcus aureus in healthy individuals has a high prevalence, especially in children and young adults. Nasal colonisation is a well-known risk factor for subsequent severe infection, or can be the source of transmission of this bacterium to other susceptible persons. In this study, we have surveyed the nasal carriage rate of students of the Semmelweis University, by screening 300 volunteers. We have determined the antibiotic sensitivity of the isolates by Etest, and their genetic relatedness by pulsed-fieled gel electrophoresis. The nasal carriage rate of S. aureus was found to be 29.3%, and that of MRSA only 0.67% (2/300). The isolates were generally sensitive to antibiotics, except for macrolides. We could observe a noticeably great genetic diversity, even among strains deriving from students of the same university group.
    MeSH term(s) Anti-Bacterial Agents/pharmacology ; Carrier State/epidemiology ; Carrier State/microbiology ; Electrophoresis, Gel, Pulsed-Field/methods ; Humans ; Hungary/epidemiology ; Microbial Sensitivity Tests/methods ; Nose/microbiology ; Prevalence ; Risk Factors ; Staphylococcal Infections/epidemiology ; Staphylococcal Infections/microbiology ; Staphylococcus aureus/genetics ; Staphylococcus aureus/isolation & purification ; Students ; Universities ; Young Adult
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2011-03
    Publishing country Hungary
    Document type Journal Article
    ZDB-ID 918256-1
    ISSN 1588-2640 ; 1217-8950
    ISSN (online) 1588-2640
    ISSN 1217-8950
    DOI 10.1556/AMicr.58.2011.1.8
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  6. Article ; Online: First description of a catalase-negative Staphylococcus aureus from a healthy carrier, with a novel nonsense mutation in the katA gene.

    Laub, Krisztina / Kristóf, Katalin / Tirczka, Tamás / Tóthpál, Adrienn / Kardos, Szilvia / Kovács, Eszter / Sahin-Tóth, Judit / Horváth, Andrea / Dobay, Orsolya

    International journal of medical microbiology : IJMM

    2017  Volume 307, Issue 8, Page(s) 431–434

    Abstract: We have screened 2568 healthy individuals (mostly children) for Staphylococcus aureus and Streptococcus pneumoniae nasal carriage between 2010 and 2012. Out of the isolated 751 S. aureus strains, we found one methicillin-sensitive catalase-negative S. ... ...

    Abstract We have screened 2568 healthy individuals (mostly children) for Staphylococcus aureus and Streptococcus pneumoniae nasal carriage between 2010 and 2012. Out of the isolated 751 S. aureus strains, we found one methicillin-sensitive catalase-negative S. aureus (CNSA). Our CNSA isolate possessed a novel nonsense point mutation in the katA gene leading to early truncation of the protein product. The strain was resistant to penicillin and erythromycin, but sensitive to all other tested antibiotics and carried the enterotoxin A gene. It belonged to sequence type 5 (ST5), which is a successful, worldwide spread, usually MRSA clone. Catalase has been described as a virulence factor strictly required for nasal colonisation, and this is the first case contradicting this theory, as all previous CNSA isolates derived from infections. This is the first report of a CNSA from a symptomless carrier as well as the first occurrence in Hungary.
    MeSH term(s) Acatalasia ; Carrier State/microbiology ; Child ; Child, Preschool ; Codon, Nonsense ; Female ; Genotype ; Humans ; Hungary ; Male ; Microbial Sensitivity Tests ; Molecular Typing ; Nasal Cavity/microbiology ; Staphylococcal Infections/microbiology ; Staphylococcus aureus/drug effects ; Staphylococcus aureus/enzymology ; Staphylococcus aureus/genetics ; Staphylococcus aureus/isolation & purification ; Young Adult
    Chemical Substances Codon, Nonsense
    Language English
    Publishing date 2017-12
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2006518-8
    ISSN 1618-0607 ; 1438-4221
    ISSN (online) 1618-0607
    ISSN 1438-4221
    DOI 10.1016/j.ijmm.2017.10.011
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  7. Article: Changes in the serotypes of Hungarian pneumococci isolated mainly from invasive infections: a review of all available data between 1988 and 2011.

    Tóthpál, Adrienn / Laub, Krisztina / Kardos, Szilvia / Nagy, Károly / Dobay, Orsolya

    Acta microbiologica et immunologica Hungarica

    2012  Volume 59, Issue 3, Page(s) 423–433

    Abstract: Streptococcus pneumoniae is responsible for a high level of morbidity and mortality, especially among children. For a long time, only the polysaccharide vaccine was available against pneumococcal infections, but in the last decade special conjugate ... ...

    Abstract Streptococcus pneumoniae is responsible for a high level of morbidity and mortality, especially among children. For a long time, only the polysaccharide vaccine was available against pneumococcal infections, but in the last decade special conjugate vaccines were developed for paediatric use. These vaccines have made a deep impact on serotype distribution all over the world, by suppressing those serotypes included in the vaccines, while new, previously rare types emerged. These changes have been monitored closely in numerous publications all over the world. Nevertheless, data on pneumococcal serotypes in Hungary were mostly published in Hungarian, therefore not available in the international literature. In this meta-analysis, our aim was to collect and summarise all available data, and try to follow the changes observed after the introduction of the conjugate vaccines.
    MeSH term(s) Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Child, Preschool ; Female ; Humans ; Hungary/epidemiology ; Infant ; Male ; Middle Aged ; Pneumococcal Infections/epidemiology ; Pneumococcal Infections/microbiology ; Pneumococcal Infections/prevention & control ; Pneumococcal Vaccines/administration & dosage ; Pneumococcal Vaccines/genetics ; Serotyping ; Streptococcus pneumoniae/classification ; Streptococcus pneumoniae/genetics ; Streptococcus pneumoniae/isolation & purification ; Young Adult
    Chemical Substances Pneumococcal Vaccines
    Language English
    Publishing date 2012-09
    Publishing country Hungary
    Document type Journal Article ; Meta-Analysis
    ZDB-ID 918256-1
    ISSN 1588-2640 ; 1217-8950
    ISSN (online) 1588-2640
    ISSN 1217-8950
    DOI 10.1556/AMicr.59.2012.3.12
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  8. Article ; Online: Radical serotype rearrangement of carried pneumococci in the first 3 years after intensive vaccination started in Hungary.

    Tóthpál, Adrienn / Kardos, Szilvia / Laub, Krisztina / Nagy, Károly / Tirczka, Tamás / van der Linden, Mark / Dobay, Orsolya

    European journal of pediatrics

    2014  Volume 174, Issue 3, Page(s) 373–381

    Abstract: Unlabelled: Streptococcus pneumoniae is responsible for a significant amount of morbidity and mortality worldwide. Healthy carriers, mainly young children, are the most important sources of infections. In the current study, we aimed to determine the ... ...

    Abstract Unlabelled: Streptococcus pneumoniae is responsible for a significant amount of morbidity and mortality worldwide. Healthy carriers, mainly young children, are the most important sources of infections. In the current study, we aimed to determine the changes that have occurred since the introduction of PCV-7 in Hungary. Nasal specimens were collected from 1,022 healthy children aged 3-6 years attending day-care centres. After thorough identification, pneumococcal isolates were serotyped, and their antibiotic sensitivity was determined. The carriage rate was found to be 34.9%. A huge serotype rearrangement was detected compared to earlier results, with the previously leading serotype 14 having completely disappeared. Serotypes 11A, 35F, 19A, 6B, 15B, 3 and 38 were most prevalent, and 29 different types were identified in total. The PCV-7 types were responsible for 16.5% of all serotypes, and 36.0% are not covered by any pneumococcal vaccines. The isolates were sensitive to most tested antibiotics, except erythromycin (resistance was 21.6%). Only one penicillin-resistant strain was found. The newly and rapidly emerging non-vaccine serotypes are much more sensitive, except serotype 19A.
    Conclusion: Due to PCV vaccination, a complete serotype arrangement occurred also in Hungary. The old "paediatric" serotypes were replaced by serotypes 11A, 35F, 19A, 6B, 15B, 3 and 38.
    MeSH term(s) Child ; Child, Preschool ; Female ; Humans ; Hungary ; Male ; Nasopharynx/microbiology ; Pneumococcal Vaccines/blood ; Pneumococcal Vaccines/immunology ; Serogroup ; Streptococcus pneumoniae/immunology ; Streptococcus pneumoniae/isolation & purification ; Vaccination/methods ; Vaccines, Conjugate/classification ; Vaccines, Conjugate/microbiology
    Chemical Substances Pneumococcal Vaccines ; Vaccines, Conjugate
    Language English
    Publishing date 2014-09-02
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 194196-3
    ISSN 1432-1076 ; 0340-6199 ; 0943-9676
    ISSN (online) 1432-1076
    ISSN 0340-6199 ; 0943-9676
    DOI 10.1007/s00431-014-2408-1
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