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  1. Article ; Online: Measurement of f_{s}/f_{u} Variation with Proton-Proton Collision Energy and B-Meson Kinematics.

    Aaij, R / Abellán Beteta, C / Adeva, B / Adinolfi, M / Aidala, C A / Ajaltouni, Z / Akar, S / Albicocco, P / Albrecht, J / Alessio, F / Alexander, M / Alfonso Albero, A / Alkhazov, G / Alvarez Cartelle, P / Alves, A A / Amato, S / Amhis, Y / An, L / Anderlini, L /
    Andreassi, G / Andreotti, M / Andrews, J E / Archilli, F / Arnau Romeu, J / Artamonov, A / Artuso, M / Arzymatov, K / Aslanides, E / Atzeni, M / Audurier, B / Bachmann, S / Back, J J / Baker, S / Balagura, V / Baldini, W / Baranov, A / Barlow, R J / Barsuk, S / Barter, W / Bartolini, M / Baryshnikov, F / Batozskaya, V / Batsukh, B / Battig, A / Battista, V / Bay, A / Bedeschi, F / Bediaga, I / Beiter, A / Bel, L J / Belavin, V / Belin, S / Beliy, N / Bellee, V / Belous, K / Belyaev, I / Bencivenni, G / Ben-Haim, E / Benson, S / Beranek, S / Berezhnoy, A / Bernet, R / Berninghoff, D / Bernstein, H C / Bertholet, E / Bertolin, A / Betancourt, C / Betti, F / Bettler, M O / Bezshyiko, Ia / Bhasin, S / Bhom, J / Bieker, M S / Bifani, S / Billoir, P / Birnkraut, A / Bizzeti, A / Bjørn, M / Blago, M P / Blake, T / Blanc, F / Blusk, S / Bobulska, D / Bocci, V / Boente Garcia, O / Boettcher, T / Boldyrev, A / Bondar, A / Bondar, N / Borghi, S / Borisyak, M / Borsato, M / Boubdir, M / Bowcock, T J V / Bozzi, C / Braun, S / Brea Rodriguez, A / Brodski, M / Brodzicka, J / Brossa Gonzalo, A / Brundu, D / Buchanan, E / Buonaura, A / Burr, C / Bursche, A / Butter, J S / Buytaert, J / Byczynski, W / Cadeddu, S / Cai, H / Calabrese, R / Cali, S / Calladine, R / Calvi, M / Calvo Gomez, M / Camboni, A / Campana, P / Campora Perez, D H / Capriotti, L / Carbone, A / Carboni, G / Cardinale, R / Cardini, A / Carniti, P / Carvalho Akiba, K / Casais Vidal, A / Casse, G / Cattaneo, M / Cavallero, G / Cenci, R / Chapman, M G / Charles, M / Charpentier, Ph / Chatzikonstantinidis, G / Chefdeville, M / Chekalina, V / Chen, C / Chen, S / Chitic, S-G / Chobanova, V / Chrzaszcz, M / Chubykin, A / Ciambrone, P / Cid Vidal, X / Ciezarek, G / Cindolo, F / Clarke, P E L / Clemencic, M / Cliff, H V / Closier, J / Cobbledick, J L / Coco, V / Coelho, J A B / Cogan, J / Cogneras, E / Cojocariu, L / Collins, P / Colombo, T / Comerma-Montells, A / Contu, A / Cooke, N / Coombs, G / Coquereau, S / Corti, G / Costa Sobral, C M / Couturier, B / Cowan, G A / Craik, D C / Crocombe, A / Cruz Torres, M / Currie, R / Da Silva, C L / Dall'Occo, E / Dalseno, J / D'Ambrosio, C / Danilina, A / d'Argent, P / Davis, A / De Aguiar Francisco, O / De Bruyn, K / De Capua, S / De Cian, M / De Miranda, J M / De Paula, L / De Serio, M / De Simone, P / de Vries, J A / Dean, C T / Dean, W / Decamp, D / Del Buono, L / Delaney, B / Dembinski, H-P / Demmer, M / Dendek, A / Denysenko, V / Derkach, D / Deschamps, O / Desse, F / Dettori, F / Dey, B / Di Canto, A / Di Nezza, P / Didenko, S / Dijkstra, H / Dordei, F / Dorigo, M / Dos Reis, A C / Dosil Suárez, A / Douglas, L / Dovbnya, A / Dreimanis, K / Dufour, L / Dujany, G / Durante, P / Durham, J M / Dutta, D / Dzhelyadin, R / Dziewiecki, M / Dziurda, A / Dzyuba, A / Easo, S / Egede, U / Egorychev, V / Eidelman, S / Eisenhardt, S / Eitschberger, U / Ekelhof, R / Ek-In, S / Eklund, L / Ely, S / Ene, A / Escher, S / Esen, S / Evans, T / Falabella, A / Färber, C / Farley, N / Farry, S / Fazzini, D / Féo, M / Fernandez Declara, P / Fernandez Prieto, A / Ferrari, F / Ferreira Lopes, L / Ferreira Rodrigues, F / Ferreres Sole, S / Ferro-Luzzi, M / Filippov, S / Fini, R A / Fiorini, M / Firlej, M / Fitzpatrick, C / Fiutowski, T / Fleuret, F / Fontana, M / Fontanelli, F / Forty, R / Franco Lima, V / Franco Sevilla, M / Frank, M / Frei, C / Fu, J / Funk, W / Gabriel, E / Gallas Torreira, A / Galli, D / Gallorini, S / Gambetta, S / Gan, Y / Gandelman, M / Gandini, P / Gao, Y / Garcia Martin, L M / García Pardiñas, J / Garcia Plana, B / Garra Tico, J / Garrido, L / Gascon, D / Gaspar, C / Gazzoni, G / Gerick, D / Gersabeck, E / Gersabeck, M / Gershon, T / Gerstel, D / Ghez, Ph / Gibson, V / Gioventù, A / Girard, O G / Gironella Gironell, P / Giubega, L / Gizdov, K / Gligorov, V V / Göbel, C / Golubkov, D / Golutvin, A / Gomes, A / Gorelov, I V / Gotti, C / Govorkova, E / Grabowski, J P / Graciani Diaz, R / Granado Cardoso, L A / Graugés, E / Graverini, E / Graziani, G / Grecu, A / Greim, R / Griffith, P / Grillo, L / Gruber, L / Gruberg Cazon, B R / Gu, C / Gushchin, E / Guth, A / Guz, Yu / Gys, T / Hadavizadeh, T / Hadjivasiliou, C / Haefeli, G / Haen, C / Haines, S C / Hamilton, P M / Han, Q / Han, X / Hancock, T H / Hansmann-Menzemer, S / Harnew, N / Harrison, T / Hasse, C / Hatch, M / He, J / Hecker, M / Heijhoff, K / Heinicke, K / Heister, A / Hennessy, K / Henry, L / Heß, M / Heuel, J / Hicheur, A / Hidalgo Charman, R / Hill, D / Hilton, M / Hopchev, P H / Hu, J / Hu, W / Huang, W / Huard, Z C / Hulsbergen, W / Humair, T / Hushchyn, M / Hutchcroft, D / Hynds, D / Ibis, P / Idzik, M / Ilten, P / Inglessi, A / Inyakin, A / Ivshin, K / Jacobsson, R / Jakobsen, S / Jalocha, J / Jans, E / Jashal, B K / Jawahery, A / Jiang, F / John, M / Johnson, D / Jones, C R / Joram, C / Jost, B / Jurik, N / Kandybei, S / Karacson, M / Kariuki, J M / Karodia, S / Kazeev, N / Kecke, M / Keizer, F / Kelsey, M / Kenzie, M / Ketel, T / Khanji, B / Kharisova, A / Khurewathanakul, C / Kim, K E / Kirn, T / Kirsebom, V S / Klaver, S / Klimaszewski, K / Koliiev, S / Kolpin, M / Kondybayeva, A / Konoplyannikov, A / Kopciewicz, P / Kopecna, R / Koppenburg, P / Kostiuk, I / Kot, O / Kotriakhova, S / Kozeiha, M / Kravchuk, L / Kreps, M / Kress, F / Kretzschmar, S / Krokovny, P / Krupa, W / Krzemien, W / Kucewicz, W / Kucharczyk, M / Kudryavtsev, V / Kunde, G J / Kuonen, A K / Kvaratskheliya, T / Lacarrere, D / Lafferty, G / Lai, A / Lancierini, D / Lane, J J / Lanfranchi, G / Langenbruch, C / Latham, T / Lazzeroni, C / Le Gac, R / Lefèvre, R / Leflat, A / Lemaitre, F / Leroy, O / Lesiak, T / Leverington, B / Li, H / Li, P-R / Li, X / Li, Y / Li, Z / Liang, X / Likhomanenko, T / Lindner, R / Lionetto, F / Lisovskyi, V / Liu, G / Liu, X / Loh, D / Loi, A / Lomba Castro, J / Longstaff, I / Lopes, J H / Loustau, G / Lovell, G H / Lucchesi, D / Lucio Martinez, M / Luo, Y / Lupato, A / Luppi, E / Lupton, O / Lusiani, A / Lyu, X / Machefert, F / Maciuc, F / Macko, V / Mackowiak, P / Maddrell-Mander, S / Maev, O / Maevskiy, A / Maguire, K / Maisuzenko, D / Majewski, M W / Malde, S / Malecki, B / Malinin, A / Maltsev, T / Malygina, H / Manca, G / Mancinelli, G / Marangotto, D / Maratas, J / Marchand, J F / Marconi, U / Marin Benito, C / Marinangeli, M / Marino, P / Marks, J / Marshall, P J / Martellotti, G / Martinazzoli, L / Martinelli, M / Martinez Santos, D / Martinez Vidal, F / Massafferri, A / Materok, M / Matev, R / Mathad, A / Mathe, Z / Matiunin, V / Matteuzzi, C / Mattioli, K R / Mauri, A / Maurice, E / Maurin, B / McCann, M / Mcconnell, L / McNab, A / McNulty, R / Mead, J V / Meadows, B / Meaux, C / Meinert, N / Melnychuk, D / Merk, M / Merli, A / Michielin, E / Milanes, D A / Millard, E / Minard, M-N / Mineev, O / Minzoni, L / Mitzel, D S / Mödden, A / Mogini, A / Moise, R D / Mombächer, T / Monroy, I A / Monteil, S / Morandin, M / Morello, G / Morello, M J / Moron, J / Morris, A B / Mountain, R / Mu, H / Muheim, F / Mukherjee, M / Mulder, M / Müller, D / Müller, J / Müller, K / Müller, V / Murphy, C H / Murray, D / Naik, P / Nakada, T / Nandakumar, R / Nandi, A / Nanut, T / Nasteva, I / Needham, M / Neri, N / Neubert, S / Neufeld, N / Newcombe, R / Nguyen, T D / Nguyen-Mau, C / Nieswand, S / Niet, R / Nikitin, N / Nolte, N S / Oblakowska-Mucha, A / Obraztsov, V / Ogilvy, S / O'Hanlon, D P / Oldeman, R / Onderwater, C J G / Osborn, J D / Ossowska, A / Otalora Goicochea, J M / Ovsiannikova, T / Owen, P / Oyanguren, A / Pais, P R / Pajero, T / Palano, A / Palutan, M / Panshin, G / Papanestis, A / Pappagallo, M / Pappalardo, L L / Parker, W / Parkes, C / Passaleva, G / Pastore, A / Patel, M / Patrignani, C / Pearce, A / Pellegrino, A / Penso, G / Pepe Altarelli, M / Perazzini, S / Pereima, D / Perret, P / Pescatore, L / Petridis, K / Petrolini, A / Petrov, A / Petrucci, S / Petruzzo, M / Pietrzyk, B / Pietrzyk, G / Pikies, M / Pili, M / Pinci, D / Pinzino, J / Pisani, F / Piucci, A / Placinta, V / Playfer, S / Plews, J / Plo Casasus, M / Polci, F / Poli Lener, M / Poliakova, M / Poluektov, A / Polukhina, N / Polyakov, I / Polycarpo, E / Pomery, G J / Ponce, S / Popov, A / Popov, D / Poslavskii, S / Prasanth, K / Price, E / Prouve, C / Pugatch, V / Puig Navarro, A / Pullen, H / Punzi, G / Qian, W / Qin, J / Quagliani, R / Quintana, B / Raab, N V / Rachwal, B / Rademacker, J H / Rama, M / Ramos Pernas, M / Rangel, M S / Ratnikov, F / Raven, G / Ravonel Salzgeber, M / Reboud, M / Redi, F / Reichert, S / Reiss, F / Remon Alepuz, C / Ren, Z / Renaudin, V / Ricciardi, S / Richards, S / Rinnert, K / Robbe, P / Robert, A / Rodrigues, A B / Rodrigues, E / Rodriguez Lopez, J A / Roehrken, M / Roiser, S / Rollings, A / Romanovskiy, V / Romero Vidal, A / Roth, J D / Rotondo, M / Rudolph, M S / Ruf, T / Ruiz Vidal, J / Saborido Silva, J J / Sagidova, N / Saitta, B / Salustino Guimaraes, V / Sanchez Gras, C / Sanchez Mayordomo, C / Sanmartin Sedes, B / Santacesaria, R / Santamarina Rios, C / Santimaria, M / Santovetti, E / Sarpis, G / Sarti, A / Satriano, C / Satta, A / Saur, M / Savrina, D / Schael, S / Schellenberg, M / Schiller, M / Schindler, H / Schmelling, M / Schmelzer, T / Schmidt, B / Schneider, O / Schopper, A / Schreiner, H F / Schubiger, M / Schulte, S / Schune, M H / Schwemmer, R / Sciascia, B / Sciubba, A / Semennikov, A / Sepulveda, E S / Sergi, A / Serra, N / Serrano, J / Sestini, L / Seuthe, A / Seyfert, P / Shapkin, M / Shears, T / Shekhtman, L / Shevchenko, V / Shmanin, E / Siddi, B G / Silva Coutinho, R / Silva de Oliveira, L / Simi, G / Simone, S / Skiba, I / Skidmore, N / Skwarnicki, T / Slater, M W / Smeaton, J G / Smith, E / Smith, I T / Smith, M / Soares, M / Soares Lavra, L / Sokoloff, M D / Soler, F J P / Souza De Paula, B / Spaan, B / Spadaro Norella, E / Spradlin, P / Stagni, F / Stahl, M / Stahl, S / Stefko, P / Stefkova, S / Steinkamp, O / Stemmle, S / Stenyakin, O / Stepanova, M / Stevens, H / Stone, S / Stracka, S / Stramaglia, M E / Straticiuc, M / Straumann, U / Strokov, S / Sun, J / Sun, L / Sun, Y / Swientek, K / Szabelski, A / Szumlak, T / Szymanski, M / Tang, Z / Tekampe, T / Tellarini, G / Teubert, F / Thomas, E / Tilley, M J / Tisserand, V / T'Jampens, S / Tobin, M / Tolk, S / Tomassetti, L / Tonelli, D / Tou, D Y / Tournefier, E / Traill, M / Tran, M T / Trisovic, A / Tsaregorodtsev, A / Tuci, G / Tully, A / Tuning, N / Ukleja, A / Usachov, A / Ustyuzhanin, A / Uwer, U / Vagner, A / Vagnoni, V / Valassi, A / Valat, S / Valenti, G / van Beuzekom, M / Van Hecke, H / van Herwijnen, E / Van Hulse, C B / van Tilburg, J / van Veghel, M / Vazquez Gomez, R / Vazquez Regueiro, P / Vázquez Sierra, C / Vecchi, S / Velthuis, J J / Veltri, M / Venkateswaran, A / Vernet, M / Veronesi, M / Vesterinen, M / Viana Barbosa, J V / Vieira, D / Vieites Diaz, M / Viemann, H / Vilasis-Cardona, X / Vitkovskiy, A / Vitti, M / Volkov, V / Vollhardt, A / Vom Bruch, D / Voneki, B / Vorobyev, A / Vorobyev, V / Voropaev, N / Waldi, R / Walsh, J / Wang, J / Wang, M / Wang, Y / Wang, Z / Ward, D R / Wark, H M / Watson, N K / Websdale, D / Weiden, A / Weisser, C / White, D J / Whitehead, M / Wilkinson, G / Wilkinson, M / Williams, I / Williams, M / Williams, M R J / Williams, T / Wilson, F F / Winn, M / Wislicki, W / Witek, M / Wormser, G / Wotton, S A / Wyllie, K / Xiang, Z / Xiao, D / Xie, Y / Xing, H / Xu, A / Xu, L / Xu, M / Xu, Q / Xu, Z / Yang, Z / Yao, Y / Yeomans, L E / Yin, H / Yu, J / Yuan, X / Yushchenko, O / Zarebski, K A / Zavertyaev, M / Zeng, M / Zhang, D / Zhang, L / Zhang, S / Zhang, W C / Zhang, Y / Zhelezov, A / Zheng, Y / Zhou, X / Zhou, Y / Zhu, X / Zhukov, V / Zonneveld, J B / Zucchelli, S

    Physical review letters

    2020  Volume 124, Issue 12, Page(s) 122002

    Abstract: The ratio of the B_{s}^{0} and B^{+} fragmentation fractions f_{s} and f_{u} is studied with B_{s ... longitudinal momentum, transverse momentum, pseudorapidity, and rapidity. The fragmentation-fraction ratio f_{s ... f_{u} is observed to depend on the B-meson transverse momentum with a significance of 6.0σ ...

    Abstract The ratio of the B_{s}^{0} and B^{+} fragmentation fractions f_{s} and f_{u} is studied with B_{s}^{0}→J/ψϕ and B^{+}→J/ψK^{+} decays using data collected by the LHCb experiment in proton-proton collisions at 7, 8, and 13 TeV center-of-mass energies. The analysis is performed in bins of B-meson momentum, longitudinal momentum, transverse momentum, pseudorapidity, and rapidity. The fragmentation-fraction ratio f_{s}/f_{u} is observed to depend on the B-meson transverse momentum with a significance of 6.0σ. This dependency is driven by the 13 TeV sample (8.7σ), while the results for the other collision energies are not significant when considered separately. Furthermore, the results show a 4.8σ evidence for an increase of f_{s}/f_{u} as a function of collision energy.
    Language English
    Publishing date 2020-04-13
    Publishing country United States
    Document type Journal Article
    ZDB-ID 208853-8
    ISSN 1079-7114 ; 0031-9007
    ISSN (online) 1079-7114
    ISSN 0031-9007
    DOI 10.1103/PhysRevLett.124.122002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Multicolor fluorescence activated cell sorting to generate humanized monoclonal antibody binding seven subtypes of BoNT/F.

    Fan, Yongfeng / Sun, Zhengda / Conrad, Fraser / Wen, Weihua / Zhao, Lequn / Lou, Jianlong / Zhou, Yu / Farr-Jones, Shauna / Marks, James D

    PloS one

    2022  Volume 17, Issue 9, Page(s) e0273512

    Abstract: ... serotype F (BoNT/F) that differ from each other in amino acid sequence by up to 36%. Previously ... binding to BoNT/F2, F5, or F7. Using multicolor labeling of the different BoNT/F subtypes and fluorescence ... to a humanized mAb hu6F15.4 that bound each of seven BoNT/F subtypes with high affinity (KD 5.81 pM to 659.78 pM ...

    Abstract Generating specific monoclonal antibodies (mAbs) that neutralize multiple antigen variants is challenging. Here, we present a strategy to generate mAbs that bind seven subtypes of botulinum neurotoxin serotype F (BoNT/F) that differ from each other in amino acid sequence by up to 36%. Previously, we identified 28H4, a mouse mAb with poor cross-reactivity to BoNT/F1, F3, F4, and F6 and with no detectable binding to BoNT/F2, F5, or F7. Using multicolor labeling of the different BoNT/F subtypes and fluorescence-activated cell sorting (FACS) of yeast displayed single-chain Fv (scFv) mutant libraries, 28H4 was evolved to a humanized mAb hu6F15.4 that bound each of seven BoNT/F subtypes with high affinity (KD 5.81 pM to 659.78 pM). In contrast, using single antigen FACS sorting, affinity was increased to the subtype used for sorting but with a decrease in affinity for other subtypes. None of the mAb variants showed any binding to other BoNT serotypes or to HEK293 or CHO cell lysates by flow cytometry, thus demonstrating stringent BoNT/F specificity. Multicolor FACS-mediated antibody library screening is thus proposed as a general method to generate multi-specific antibodies to protein subtypes such as toxins or species variants.
    MeSH term(s) Animals ; Humans ; Mice ; Antibodies, Monoclonal/chemistry ; Antibodies, Monoclonal, Humanized/chemistry ; Botulinum Toxins/immunology ; Cross Reactions ; Flow Cytometry/methods ; HEK293 Cells ; Single-Chain Antibodies/chemistry
    Chemical Substances Antibodies, Monoclonal ; Antibodies, Monoclonal, Humanized ; Botulinum Toxins (EC 3.4.24.69) ; Single-Chain Antibodies ; botulinum toxin type F (U1R2P71O7G)
    Language English
    Publishing date 2022-09-01
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0273512
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Book ; Online: Precise measurement of the $f_s/f_d$ ratio of fragmentation fractions and of $B^0_s$ decay branching fractions

    LHCb collaboration / Aaij, R. / Beteta, C. Abellán / Ackernley, T. / Adeva, B. / Adinolfi, M. / Afsharnia, H. / Aidala, C. A. / Aiola, S. / Ajaltouni, Z. / Akar, S. / Albrecht, J. / Alessio, F. / Alexander, M. / Albero, A. Alfonso / Aliouche, Z. / Alkhazov, G. / Cartelle, P. Alvarez / Amato, S. /
    Amhis, Y. / An, L. / Anderlini, L. / Andreianov, A. / Andreotti, M. / Archilli, F. / Artamonov, A. / Artuso, M. / Arzymatov, K. / Aslanides, E. / Atzeni, M. / Audurier, B. / Bachmann, S. / Bachmayer, M. / Back, J. J. / Baker, S. / Rodriguez, P. Baladron / Balagura, V. / Baldini, W. / Leite, J. Baptista / Barlow, R. J. / Barsuk, S. / Barter, W. / Bartolini, M. / Baryshnikov, F. / Basels, J. M. / Bassi, G. / Batsukh, B. / Battig, A. / Bay, A. / Becker, M. / Bedeschi, F. / Bediaga, I. / Beiter, A. / Belavin, V. / Belin, S. / Bellee, V. / Belous, K. / Belov, I. / Belyaev, I. / Bencivenni, G. / Ben-Haim, E. / Berezhnoy, A. / Bernet, R. / Berninghoff, D. / Bernstein, H. C. / Bertella, C. / Bertolin, A. / Betancourt, C. / Betti, F. / Bezshyiko, Ia. / Bhasin, S. / Bhom, J. / Bian, L. / Bieker, M. S. / Bifani, S. / Billoir, P. / Birch, M. / Bishop, F. C. R. / Bitadze, A. / Bizzeti, A. / Bjørn, M. / Blago, M. P. / Blake, T. / Blanc, F. / Blusk, S. / Bobulska, D. / Boelhauve, J. A. / Garcia, O. Boente / Boettcher, T. / Boldyrev, A. / Bondar, A. / Bondar, N. / Borghi, S. / Borisyak, M. / Borsato, M. / Borsuk, J. T. / Bouchiba, S. A. / Bowcock, T. J. V. / Boyer, A. / Bozzi, C. / Bradley, M. J. / Braun, S. / Rodriguez, A. Brea / Brodski, M. / Brodzicka, J. / Gonzalo, A. Brossa / Brundu, D. / Buonaura, A. / Burr, C. / Bursche, A. / Butkevich, A. / Butter, J. S. / Buytaert, J. / Byczynski, W. / Cadeddu, S. / Cai, H. / Calabrese, R. / Calefice, L. / Diaz, L. Calero / Cali, S. / Calladine, R. / Calvi, M. / Gomez, M. Calvo / Magalhaes, P. Camargo / Camboni, A. / Campana, P. / Quezada, A. F. Campoverde / Capelli, S. / Capriotti, L. / Carbone, A. / Carboni, G. / Cardinale, R. / Cardini, A. / Carli, I. / Carniti, P. / Carus, L. / Akiba, K. Carvalho / Vidal, A. Casais / Casse, G. / Cattaneo, M. / Cavallero, G. / Celani, S. / Cerasoli, J. / Chadwick, A. J. / Chapman, M. G. / Charles, M. / Charpentier, Ph. / Chatzikonstantinidis, G. / Barajas, C. A. Chavez / Chefdeville, M. / Chen, C. / Chen, S. / Chernov, A. / Chobanova, V. / Cholak, S. / Chrzaszcz, M. / Chubykin, A. / Chulikov, V. / Ciambrone, P. / Cicala, M. F. / Vidal, X. Cid / Ciezarek, G. / Clarke, P. E. L. / Clemencic, M. / Cliff, H. V. / Closier, J. / Cobbledick, J. L. / Coco, V. / Coelho, J. A. B. / Cogan, J. / Cogneras, E. / Cojocariu, L. / Collins, P. / Colombo, T. / Congedo, L. / Contu, A. / Cooke, N. / Coombs, G. / Corti, G. / Sobral, C. M. Costa / Couturier, B. / Craik, D. C. / Crkovská, J. / Torres, M. Cruz / Currie, R. / Da Silva, C. L. / Dall'Occo, E. / Dalseno, J. / D'Ambrosio, C. / Danilina, A. / d'Argent, P. / Davis, A. / Francisco, O. De Aguiar / De Bruyn, K. / De Capua, S. / De Cian, M. / De Miranda, J. M. / De Paula, L. / De Serio, M. / De Simone, D. / De Simone, P. / de Vries, J. A. / Dean, C. 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    2021  

    Abstract: The ratio of the $B^0_s$ and $B^0$ fragmentation fractions, $f_s/f_d$, in proton-proton collisions ... also updated using the new values of $f_s/f_d$ and branching fractions of normalisation channels ...

    Abstract The ratio of the $B^0_s$ and $B^0$ fragmentation fractions, $f_s/f_d$, in proton-proton collisions at the LHC, is obtained as a function of $B$-meson transverse momentum and collision centre-of-mass energy from the combined analysis of different $B$-decay channels measured by the LHCb experiment. The results are described by a linear function of the meson transverse momentum, or with a function inspired by Tsallis statistics. Precise measurements of the branching fractions of the $B^0_s \to J/\psi \phi$ and $B^0_s \to D^-_s \pi^+$ decays are performed, reducing their uncertainty by about a factor of two with respect to previous world averages. Numerous $B^0_s$ decay branching fractions, measured at the LHCb experiment, are also updated using the new values of $f_s/f_d$ and branching fractions of normalisation channels. These results reduce a major source of systematic uncertainty in several searches for new physics performed through measurements of $B^0_s$ branching fractions.

    Comment: All figures and tables, along with any supplementary material and additional information, are available at https://cern.ch/lhcbproject/Publications/p/LHCb-PAPER-2020-046.html (LHCb public pages)
    Keywords High Energy Physics - Experiment
    Subject code 612
    Publishing date 2021-03-11
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Impact of abiotic and biogeochemical processes on halogen concentrations (Cl, Br, F, I) in mineral soil along a climatic gradient.

    Neidhardt, Harald / Lemke, Erik / Epp, Tatjana / Marks, Michael A W / Markl, Gregor / Oelmann, Yvonne

    Environmental science. Processes & impacts

    2022  Volume 24, Issue 9, Page(s) 1330–1342

    Abstract: ... of fluorine (F), chlorine (Cl), bromine (Br) and iodine (I) has been shown for temperate forests. To further ... ion chromatography. Highest average total halogen concentrations occurred at the arid site (Cl, F: 4270 and 897 mg kg ...

    Abstract In contrast to earlier ideas that halogens behave inertly in soil, extensive biogeochemical cycling of fluorine (F), chlorine (Cl), bromine (Br) and iodine (I) has been shown for temperate forests. To further advance our understanding of halogen behaviour in soil beyond humid temperate forests, we sampled soil profiles in protected areas along the Chilean Coastal Cordillera, representing a pronounced climatic gradient spanning from arid to humid. Halogen concentrations in soil were analysed by combustion ion chromatography. Highest average total halogen concentrations occurred at the arid site (Cl, F: 4270 and 897 mg kg
    MeSH term(s) Bromine/chemistry ; Chlorine/chemistry ; Fluorine ; Halogens/analysis ; Iodine ; Minerals ; Soil
    Chemical Substances Halogens ; Minerals ; Soil ; Fluorine (284SYP0193) ; Chlorine (4R7X1O2820) ; Iodine (9679TC07X4) ; Bromine (SBV4XY874G)
    Language English
    Publishing date 2022-09-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 2703814-2
    ISSN 2050-7895 ; 2050-7887
    ISSN (online) 2050-7895
    ISSN 2050-7887
    DOI 10.1039/d2em00015f
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  5. Article ; Online: Branching Fraction Measurements of the Rare B_{s}^{0}→ϕμ^{+}μ^{-} and B_{s}^{0}→f_{2}^{'}(1525)μ^{+}μ^{-} Decays.

    Aaij, R / Abellán Beteta, C / Ackernley, T / Adeva, B / Adinolfi, M / Afsharnia, H / Aidala, C A / Aiola, S / Ajaltouni, Z / Akar, S / Albrecht, J / Alessio, F / Alexander, M / Alfonso Albero, A / Aliouche, Z / Alkhazov, G / Alvarez Cartelle, P / Amato, S / Amhis, Y /
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    Physical review letters

    2021  Volume 127, Issue 15, Page(s) 151801

    Abstract: ... 0}→f_{2}^{'}(1525)μ^{+}μ^{-} decay is reported with a statistical significance of 9 standard ...

    Abstract The branching fraction of the rare B_{s}^{0}→ϕμ^{+}μ^{-} decay is measured using data collected by the LHCb experiment at center-of-mass energies of 7, 8, and 13 TeV, corresponding to integrated luminosities of 1, 2, and 6  fb^{-1}, respectively. The branching fraction is reported in intervals of q^{2}, the square of the dimuon invariant mass. In the q^{2} region between 1.1 and 6.0  GeV^{2}/c^{4}, the measurement is found to lie 3.6 standard deviations below a standard model prediction based on a combination of light cone sum rule and lattice QCD calculations. In addition, the first observation of the rare B_{s}^{0}→f_{2}^{'}(1525)μ^{+}μ^{-} decay is reported with a statistical significance of 9 standard deviations and its branching fraction is determined.
    Language English
    Publishing date 2021-10-22
    Publishing country United States
    Document type Journal Article
    ZDB-ID 208853-8
    ISSN 1079-7114 ; 0031-9007
    ISSN (online) 1079-7114
    ISSN 0031-9007
    DOI 10.1103/PhysRevLett.127.151801
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Impact of abiotic and biogeochemical processes on halogen concentrations (Cl, Br, F, I) in mineral soil along a climatic gradient

    Neidhardt, Harald / Lemke, Erik / Epp, Tatjana / Marks, Michael A. W. / Markl, Gregor / Oelmann, Yvonne

    Environmental science. 2022 Sept. 21, v. 24, no. 9

    2022  

    Abstract: ... of fluorine (F), chlorine (Cl), bromine (Br) and iodine (I) has been shown for temperate forests. To further ... ion chromatography. Highest average total halogen concentrations occurred at the arid site (Cl, F: 4270 and 897 mg ... depth distributions of F, Cl, Br and I occur in the soil. In summary, our findings provide new insights ...

    Abstract In contrast to earlier ideas that halogens behave inertly in soil, extensive biogeochemical cycling of fluorine (F), chlorine (Cl), bromine (Br) and iodine (I) has been shown for temperate forests. To further advance our understanding of halogen behaviour in soil beyond humid temperate forests, we sampled soil profiles in protected areas along the Chilean Coastal Cordillera, representing a pronounced climatic gradient spanning from arid to humid. Halogen concentrations in soil were analysed by combustion ion chromatography. Highest average total halogen concentrations occurred at the arid site (Cl, F: 4270 and 897 mg kg⁻¹) as well as the humid end of the climatic gradient (Br, I: 42.6 and 9.8 mg kg⁻¹). Vertical distribution patterns of halogens were most pronounced at the humid end of the gradient and became less distinct under drier climate. The climatic gradient demonstrates the important role of biotic processes (e.g. the halogenation of organic matter) on the retention of halogens in the soil. However, this climate-specific role may be overridden by mainly abiotic processes within a given climate zone (e.g. weathering, leaching, sorption to secondary soil minerals, evaporative enrichment), resulting in vertical relocation of halogens in the soil. Since some of these processes oppose each other, complex interactions and depth distributions of F, Cl, Br and I occur in the soil. In summary, our findings provide new insights into the fate of halogens in mineral soil of different climatic zones, which is important, for example, when radiohalogens are deposited on a large scale after nuclear accidents.
    Keywords bromine ; chlorine ; climatic zones ; combustion ; environmental science ; fluorine ; iodine ; ion exchange chromatography ; mineral soils ; organic matter ; sorption ; spatial distribution
    Language English
    Dates of publication 2022-0921
    Size p. 1330-1342.
    Publishing place The Royal Society of Chemistry
    Document type Article
    ZDB-ID 2703814-2
    ISSN 2050-7895 ; 2050-7887
    ISSN (online) 2050-7895
    ISSN 2050-7887
    DOI 10.1039/d2em00015f
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Multicolor fluorescence activated cell sorting to generate humanized monoclonal antibody binding seven subtypes of BoNT/F.

    Yongfeng Fan / Zhengda Sun / Fraser Conrad / Weihua Wen / Lequn Zhao / Jianlong Lou / Yu Zhou / Shauna Farr-Jones / James D Marks

    PLoS ONE, Vol 17, Iss 9, p e

    2022  Volume 0273512

    Abstract: ... serotype F (BoNT/F) that differ from each other in amino acid sequence by up to 36%. Previously ... binding to BoNT/F2, F5, or F7. Using multicolor labeling of the different BoNT/F subtypes and fluorescence ... to a humanized mAb hu6F15.4 that bound each of seven BoNT/F subtypes with high affinity (KD 5.81 pM to 659.78 pM ...

    Abstract Generating specific monoclonal antibodies (mAbs) that neutralize multiple antigen variants is challenging. Here, we present a strategy to generate mAbs that bind seven subtypes of botulinum neurotoxin serotype F (BoNT/F) that differ from each other in amino acid sequence by up to 36%. Previously, we identified 28H4, a mouse mAb with poor cross-reactivity to BoNT/F1, F3, F4, and F6 and with no detectable binding to BoNT/F2, F5, or F7. Using multicolor labeling of the different BoNT/F subtypes and fluorescence-activated cell sorting (FACS) of yeast displayed single-chain Fv (scFv) mutant libraries, 28H4 was evolved to a humanized mAb hu6F15.4 that bound each of seven BoNT/F subtypes with high affinity (KD 5.81 pM to 659.78 pM). In contrast, using single antigen FACS sorting, affinity was increased to the subtype used for sorting but with a decrease in affinity for other subtypes. None of the mAb variants showed any binding to other BoNT serotypes or to HEK293 or CHO cell lysates by flow cytometry, thus demonstrating stringent BoNT/F specificity. Multicolor FACS-mediated antibody library screening is thus proposed as a general method to generate multi-specific antibodies to protein subtypes such as toxins or species variants.
    Keywords Medicine ; R ; Science ; Q
    Subject code 570
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: A three monoclonal antibody combination potently neutralizes multiple botulinum neurotoxin serotype F subtypes.

    Fan, Yongfeng / Garcia-Rodriguez, Consuelo / Lou, Jianlong / Wen, Weihua / Conrad, Fraser / Zhai, Wenwu / Smith, Theresa J / Smith, Leonard A / Marks, James D

    PloS one

    2017  Volume 12, Issue 3, Page(s) e0174187

    Abstract: ... with around 1% caused by serotype F (BoNT/F). BoNT/F comprises at least seven different subtypes ... challenge for generating monoclonal antibodies (mAbs) that can bind, detect and neutralize all BoNT/F ... to generate a panel of 33 lead single chain Fv (scFv) mAbs that bound one or more BoNT/F subtypes ...

    Abstract Human botulism is primarily caused by botulinum neurotoxin (BoNT) serotypes A, B and E, with around 1% caused by serotype F (BoNT/F). BoNT/F comprises at least seven different subtypes with the amino acid sequence difference between subtypes as high as 36%. The sequence differences present a significant challenge for generating monoclonal antibodies (mAbs) that can bind, detect and neutralize all BoNT/F subtypes. We used repertoire cloning of immune mouse antibody variable (V) regions and yeast display to generate a panel of 33 lead single chain Fv (scFv) mAbs that bound one or more BoNT/F subtypes with a median equilibrium dissociation constant (KD) of 4.06 × 10-9 M. By diversifying the V-regions of the lead mAbs and selecting for cross reactivity we generated five mAbs that bound each of the seven subtypes. Three scFv binding non-overlapping epitopes were converted to IgG that had KD for the different BoNT/F subtypes ranging from 2.2×10-8 M to 1.47×10-12 pM. An equimolar combination of the mAbs was able to potently neutralize BoNT/F1, F2, F4 and F7 in the mouse neutralization assay. The mAbs have potential utility as diagnostics capable of recognizing the known BoNT/F subtypes and could be developed as antitoxins to prevent and treat type F botulism.
    MeSH term(s) Amino Acid Sequence ; Animals ; Antibodies, Monoclonal/immunology ; Antitoxins/genetics ; Antitoxins/immunology ; Botulinum Toxins/immunology ; Botulism/diagnosis ; Botulism/therapy ; Catalytic Domain/immunology ; Clostridium botulinum/metabolism ; Cross Reactions/immunology ; Epitope Mapping ; Epitopes/immunology ; Escherichia coli/genetics ; Immunization ; Mice ; Saccharomyces cerevisiae/genetics ; Single-Chain Antibodies/genetics ; Single-Chain Antibodies/immunology
    Chemical Substances Antibodies, Monoclonal ; Antitoxins ; Epitopes ; Single-Chain Antibodies ; Botulinum Toxins (EC 3.4.24.69) ; botulinum toxin type F (U1R2P71O7G)
    Language English
    Publishing date 2017-03-21
    Publishing country United States
    Document type Journal Article
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0174187
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Distribuição espacial e estrutura populacional de Pilosocereus pachycladus F. Ritter subsp. pernambucoensis (F. Ritter) Zappi e Cereus jamacaru DC. subsp. jamacaru

    Ivan Sérgio da Silva Oliveira / Vênia Camelo de Souza / Zelma Glebya Maciel Quirino / Paulo Marks de Araújo Costa / Alex da Silva Barbosa / Daniel da Silva Gomes

    Research, Society and Development, Vol 9, Iss

    2020  Volume 10

    Abstract: ... sobre a distribuição espacial e estrutura populacional de Pilosocereus pachycladus F. Ritter subsp. pernambucoensis (F. Ritter ...

    Abstract A família Cactaceae apresenta mais de 1.500 espécies no mundo, ocorrendo espécies endêmicas em todo o território nacional. Em populações naturais, tais elementos sofrem fortes pressões antrópicas, o que aumenta consideravelmente o seu risco de extinção. O objetivo do trabalho foi realizar um estudo sobre a distribuição espacial e estrutura populacional de Pilosocereus pachycladus F. Ritter subsp. pernambucoensis (F. Ritter) Zappi e Cereus jamacaru DC. subsp. jamacaru em duas áreas de Caatinga do Agreste do estado da Paraíba, Brasil. Os ambientes de estudo estão localizados no Instituto Nacional do Semiárido (INSA), Campina Grande-PB, e no Sítio Umburana, Bananeiras-PB. Foram alocadas 10 parcelas amostrais de 400 m² em cada ambiente de estudo, e todos os indivíduos de P. pachycladus subsp. pernambucoensis e C. jamacaru subsp. jamacaru foram contabilizados. A distribuição espacial das espécies, não apresentaram tendência ao agrupamento nas populações localizadas no INSA. Nas populações do Sítio Umburana, a tendência é ao agrupamento. De acordo com índice de agrupamento de Payandeh (Pi) a densidade populacional e absoluta nas populações do Sítio Umburana é maior, se comparado às populações do INSA. Para os padrões espaciais, nas populações de Cactaceae do Sítio Umburana, os indivíduos de ambas as espécies apresentam distribuição agrupada e nas populações do INSA apresentaram a tendência ao não agrupamento. Fatores como distúrbios naturais ou antrópicos, condições ambientais e disponibilidade de recursos, influenciaram a estrutura populacional de P. pachycladus e C. jamacaru.
    Keywords Caatinga ; Cactáceas ; Ecologia ; Facheiro ; Mandacaru ; Special aspects of education ; LC8-6691 ; Science (General) ; Q1-390 ; Social Sciences ; H
    Language English
    Publishing date 2020-09-01T00:00:00Z
    Publisher Grupo de Pesquisa Metodologias em Ensino e Aprendizagem em Ciências
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Determination of f(s)/f(d) for 7 TeV pp collisions and measurement of the B0→D-K+ branching fraction.

    Aaij, R / Adeva, B / Adinolfi, M / Adrover, C / Affolder, A / Ajaltouni, Z / Albrecht, J / Alessio, F / Alexander, M / Alkhazov, G / Alvarez Cartelle, P / Alves, A A / Amato, S / Amhis, Y / Amoraal, J / Anderson, J / Appleby, R B / Gutierrez, O Aquines / Arrabito, L /
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N / Vidal, X Cid / Clarke, P E L / Clemencic, M / Cliff, H V / Closier, J / Coca, C / Coco, V / Cogan, J / Collins, P / Constantin, F / Conti, G / Contu, A / Cook, A / Coombes, M / Corti, G / Cowan, G A / Currie, R / D'Almagne, B / D'Ambrosio, C / David, P / David, P N Y / De Bonis, I / De Capua, S / De Cian, M / De Lorenzi, F / De Miranda, J M / De Paula, L / De Simone, P / Decamp, D / Deckenhoff, M / Degaudenzi, H / Deissenroth, M / Del Buono, L / Deplano, C / Deschamps, O / Dettori, F / Dickens, J / Dijkstra, H / Batista, P Diniz / Dossett, D / Dovbnya, A / Dupertuis, F / Dzhelyadin, R / Eames, C / Easo, S / Egede, U / Egorychev, V / Eidelman, S / van Eijk, D / Eisele, F / Eisenhardt, S / Ekelhof, R / Eklund, L / Elsasser, Ch / d'Enterria, D G / Pereira, D Esperante / Estève, L / Falabella, A / Fanchini, E / Färber, C / Fardell, G / Farinelli, C / Farry, S / Fave, V / Albor, V Fernandez / Ferro-Luzzi, M / Filippov, S / Fitzpatrick, C / Fontana, M / Fontanelli, F / Forty, R / Frank, M / Frei, C / Frosini, M / Furcas, S / Torreira, A Gallas / Galli, D / Gandelman, M / Gandini, P / Gao, Y / Garnier, J-C / Garofoli, J / Garrido, L / Gaspar, C / Gauvin, N / Gersabeck, M / Gershon, T / Ghez, Ph / Gibson, V / Gligorov, V V / Göbel, C / Golubkov, D / Golutvin, A / Gomes, A / Gordon, H / Gándara, M Grabalosa / Diaz, R Graciani / Cardoso, L A Granado / Graugés, E / Graziani, G / Grecu, A / Gregson, S / Gui, B / Gushchin, E / Guz, Yu / Gys, T / Haefeli, G / Haines, S C / Hampson, T / Hansmann-Menzemer, S / Harji, R / Harnew, N / Harrison, J / Harrison, P F / He, J / Heijne, V / Hennessy, K / Henrard, P / Morata, J A Hernando / van Herwijnen, E / Hofmann, W / Holubyev, K / Hopchev, P / Hulsbergen, W / Hunt, P / Huse, T / Huston, R S / Hutchcroft, D / Hynds, D / Iakovenko, V / Ilten, P / Imong, J / Jacobsson, R / Hussein, M Jahjah / Jans, E / Jansen, F / Jaton, P / Jean-Marie, B / Jing, F / John, M / Johnson, D / Jones, C R / Jost, B / Kandybei, S / Karbach, T M / Keaveney, J 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Wiggers, L / Wilkinson, G / Williams, M P / Williams, M / Wilson, F F / Wishahi, J / Witek, M / Witzeling, W / Wotton, S A / Wyllie, K / Xie, Y / Xing, F / Yang, Z / Young, R / Yushchenko, O / Zavertyaev, M / Zhang, L / Zhang, W C / Zhang, Y / Zhelezov, A / Zhong, L / Zverev, E / Zvyagin, A

    Physical review letters

    2011  Volume 107, Issue 21, Page(s) 211801

    Abstract: ... 18(stat) ± 0.14(syst)) × 10(-4). The ratio of fragmentation fractions f(s)/f(d) is determined ... through the relative abundance of B(s)(0)→D(s)(-)π(+) to B(0)→D(-)K(+) and B(0)→D(-)π(+), leading to f(s)/f(d) = 0.253 ± 0.017 ...

    Abstract The relative abundance of the three decay modes B(0)→D(-)K(+), B(0)→D(-)π(+), and B(s)(0)→D(s)(-)π(+) produced in 7 TeV pp collisions at the LHC is determined from data corresponding to an integrated luminosity of 35 pb(-1). The branching fraction of B(0)→D(-)K(+) is found to be B(B(0)→D(-)K(+)) = (2.01 ± 0.18(stat) ± 0.14(syst)) × 10(-4). The ratio of fragmentation fractions f(s)/f(d) is determined through the relative abundance of B(s)(0)→D(s)(-)π(+) to B(0)→D(-)K(+) and B(0)→D(-)π(+), leading to f(s)/f(d) = 0.253 ± 0.017 ± 0.017 ± 0.020, where the uncertainties are statistical, systematic, and theoretical, respectively.
    Language English
    Publishing date 2011-11-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 208853-8
    ISSN 1079-7114 ; 0031-9007
    ISSN (online) 1079-7114
    ISSN 0031-9007
    DOI 10.1103/PhysRevLett.107.211801
    Database MEDical Literature Analysis and Retrieval System OnLINE

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