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  1. Article ; Online: Separation of Double- and Single-Stranded Nucleic Acids Using Hydroxylapatite Chromatography: Preparation and Analysis of DNA.

    Richards, Eric J

    Current protocols in molecular biology

    2021  Volume 4, Issue 1, Page(s) 2.10.1–2.10.3

    Language English
    Publishing date 2021-07-13
    Publishing country United States
    Document type Journal Article
    ISSN 1934-3647
    ISSN (online) 1934-3647
    DOI 10.1002/j.1934-3647.1988.tb00185.x
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  2. Article ; Online: Separation of Double- and Single-Stranded Nucleic Acids Using Hydroxylapatite Chromatography: Preparation and Analysis of DNA.

    Richards, Eric J

    Current protocols in molecular biology

    2021  Volume 6, Issue 1, Page(s) 2.10.1–2.10.3

    Language English
    Publishing date 2021-07-13
    Publishing country United States
    Document type Journal Article
    ISSN 1934-3647
    ISSN (online) 1934-3647
    DOI 10.1002/j.1934-3647.1989.tb00189.x
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  3. Article ; Online: The edge of the nucleus: Variations on a theme.

    Choi, Junsik / Richards, Eric J

    Developmental cell

    2022  Volume 57, Issue 1, Page(s) 3–4

    Abstract: The plant nuclear lamina utilizes distinct and highly divergent proteins to mediate chromatin interactions at the nuclear edge. In this issue of Developmental Cell, Tang et al. show that members of PNET2, a family of inner nuclear membrane proteins in ... ...

    Abstract The plant nuclear lamina utilizes distinct and highly divergent proteins to mediate chromatin interactions at the nuclear edge. In this issue of Developmental Cell, Tang et al. show that members of PNET2, a family of inner nuclear membrane proteins in Arabidopsis, are capable of binding histones and are involved in large-scale genome organization.
    MeSH term(s) Cell Nucleus ; Chromatin ; Histones ; Nuclear Envelope ; Nuclear Lamina
    Chemical Substances Chromatin ; Histones
    Language English
    Publishing date 2022-01-11
    Publishing country United States
    Document type Journal Article ; Comment
    ZDB-ID 2054967-2
    ISSN 1878-1551 ; 1534-5807
    ISSN (online) 1878-1551
    ISSN 1534-5807
    DOI 10.1016/j.devcel.2021.12.013
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5742: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 76: Show issues

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  4. Article ; Online: Seminars in cell and development biology on histone variants remodelers of H2A variants associated with heterochromatin.

    Berger, Frédéric / Muegge, Kathrin / Richards, Eric J

    Seminars in cell & developmental biology

    2022  Volume 135, Page(s) 93–101

    Abstract: Variants of the histone H2A occupy distinct locations in the genome. There is relatively little known about the mechanisms responsible for deposition of specific H2A variants. Notable exceptions are chromatin remodelers that control the dynamics of H2A.Z ...

    Abstract Variants of the histone H2A occupy distinct locations in the genome. There is relatively little known about the mechanisms responsible for deposition of specific H2A variants. Notable exceptions are chromatin remodelers that control the dynamics of H2A.Z at promoters. Here we review the steps that identified the role of a specific class of chromatin remodelers, including LSH and DDM1 that deposit the variants macroH2A in mammals and H2A.W in plants, respectively. The function of these remodelers in heterochromatin is discussed together with their multiple roles in genome stability.
    MeSH term(s) Animals ; Histones/genetics ; Histones/metabolism ; Heterochromatin/genetics ; Chromatin ; Promoter Regions, Genetic ; Mammals/genetics ; Biology ; Nucleosomes
    Chemical Substances Histones ; Heterochromatin ; Chromatin ; Nucleosomes
    Language English
    Publishing date 2022-03-04
    Publishing country England
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural ; Research Support, N.I.H., Intramural
    ZDB-ID 1312473-0
    ISSN 1096-3634 ; 1084-9521
    ISSN (online) 1096-3634
    ISSN 1084-9521
    DOI 10.1016/j.semcdb.2022.02.026
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    Zs.A 2918: Show issues Location:
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  5. Article ; Online: The role of CRWN nuclear proteins in chromatin-based regulation of stress response genes.

    Choi, Junsik / Richards, Eric J

    Plant signaling & behavior

    2019  Volume 15, Issue 1, Page(s) 1694224

    Abstract: The periphery in animal nuclei is generally considered to be a transcriptionally repressive environment. Recent studies indicate that chromatin-based mechanisms establish a similar situation in plant nuclei. We demonstrated recently that the loss of CRWN ...

    Abstract The periphery in animal nuclei is generally considered to be a transcriptionally repressive environment. Recent studies indicate that chromatin-based mechanisms establish a similar situation in plant nuclei. We demonstrated recently that the loss of CRWN nuclear lamina proteins in Arabidopsis leads to the misregulation of a group of genes involved in plant defense. How this defense response is triggered is largely unknown. Here, we briefly review recent findings that identify several layers of chromatin-based regulation responsible for this response. Further, we introduce new data suggesting that histone H3 lysine 27 tri-methylation levels are reduced in the absence of CRWNs near genes encoding transcription factors regulating SA biosynthesis, providing an explanation for SA induction. These discoveries begin to uncover the interplay between nuclear architecture and stress response in plants.
    MeSH term(s) Arabidopsis/genetics ; Arabidopsis/metabolism ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Chromatin/genetics ; Chromatin/metabolism ; Gene Expression Regulation, Plant ; Histones/metabolism ; Nuclear Proteins/genetics ; Nuclear Proteins/metabolism ; Transcription Factors/metabolism
    Chemical Substances Arabidopsis Proteins ; Chromatin ; Histones ; Nuclear Proteins ; Transcription Factors
    Language English
    Publishing date 2019-11-22
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ISSN 1559-2324
    ISSN (online) 1559-2324
    DOI 10.1080/15592324.2019.1694224
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  6. Book ; Online: Implementing an Electronic Health Record System

    Walker, James M. / Bieber, Eric J. / Richards, Frank

    2005  

    Author's details edited by James M. Walker, Eric J. Bieber, Frank Richards
    Keywords Medical records/Data processing ; Public health
    Language English
    Publisher Springer-Verlag London Limited
    Publishing place London
    Document type Book ; Online
    HBZ-ID TT050387305
    ISBN 978-1-85233-826-8 ; 978-1-8462-8115-0 ; 1-85233-826-1 ; 1-8462-8115-6
    DOI 10.1007/b138849
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  7. Article ; Online: Microscopic Dynamics of Inverse Wormlike Micelles Probed Using X-ray Photon Correlation Spectroscopy.

    Cho, Noah H / Zhang, Qingteng / Dufresne, Eric M / Narayanan, Suresh / Richards, Jeffrey J

    ACS macro letters

    2022  Volume 11, Issue 4, Page(s) 575–579

    Abstract: Wormlike micelles (WLMs) are ubiquitous viscoelastic modifiers that share properties with polymer solutions. While their macroscopic rheology is well-understood, their microscopic dynamics remain difficult to measure because they span a large range of ... ...

    Abstract Wormlike micelles (WLMs) are ubiquitous viscoelastic modifiers that share properties with polymer solutions. While their macroscopic rheology is well-understood, their microscopic dynamics remain difficult to measure because they span a large range of time and length scales. In this work, we demonstrate the use of X-ray photon correlation spectroscopy to interrogate the segmental dynamics of inverse WLM solutions swollen with a rubidium chloride solution. We observe a diffusive scaling of the dynamics and extract a temperature-dependent diffusion coefficient, which we associate with the thermal interactions of the slow segmental dynamics near entanglement points. We probe this relaxation process across the unbranched to branched topological transition and find no microstructural evidence of branch formation in the slow mode. Instead, we observe that the dynamics become more homogeneous and prominent as the temperature is reduced and water content increases.
    MeSH term(s) Micelles ; Rheology ; Spectrum Analysis ; Surface-Active Agents/chemistry ; X-Rays
    Chemical Substances Micelles ; Surface-Active Agents
    Language English
    Publishing date 2022-04-07
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2161-1653
    ISSN (online) 2161-1653
    DOI 10.1021/acsmacrolett.1c00651
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  8. Article: Radiology Performed Fluoroscopy-Guided Lumbar Punctures Decrease Volume of Diagnostic Study Interpretation - Impact on Resident Training and Potential Solutions.

    Richards, Tyler John / Schmitt, James Eric / Wolansky, Leo J / Nayate, Ameya P

    Journal of clinical imaging science

    2021  Volume 11, Page(s) 39

    Abstract: Objectives: Lumbar punctures performed in radiology departments have significantly increased over the last few decades and are typically performed in academic centers by radiology trainees using fluoroscopy guidance. Performing fluoroscopy-guided lumbar ...

    Abstract Objectives: Lumbar punctures performed in radiology departments have significantly increased over the last few decades and are typically performed in academic centers by radiology trainees using fluoroscopy guidance. Performing fluoroscopy-guided lumbar punctures (FGLPs) can often constitute a large portion of a trainee's workday and the impact of performing FGLPs on the trainee's clinical productivity (i.e. dictating reports on neuroradiology cross-sectional imaging) has not been studied. The purpose of the study was to evaluate the relationship between the number of FGLPs performed and cross-sectional neuroimaging studies dictated by residents during their neuroradiology rotation (NR).
    Material and methods: The number of FGLPs and myelograms performed and neuroimaging studies dictated by radiology residents on our neuroradiology service from July 2008 to December 2017 were retrospectively reviewed. The relationship between the number of FGLPs performed and neuroimaging studies (CT and MRI) dictated per day by residents was examined.
    Results: Radiology residents (
    Conclusion: We quantified the impact performing FGLPs can have on the number of neuroimaging reports residents dictate on the NR. We described solutions to potentially decrease unnecessary FGLP referrals including establishing departmental guidelines for FGLP referrals and encouraging bedside lumbar punctures attempts before referral. We also emphasized equally distributing the FGLPs among trainees to mitigate procedural burden.
    Language English
    Publishing date 2021-07-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2601233-9
    ISSN 2156-5597 ; 2156-7514
    ISSN (online) 2156-5597
    ISSN 2156-7514
    DOI 10.25259/JCIS_2_2021
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  9. Article ; Online: A detailed examination of pitch discrimination deficits associated with auditory verbal hallucinations in schizophrenia.

    Richards, Sophie E / Sumner, Philip J / Tan, Eric J / Meyer, Denny / Rossell, Susan L / Carruthers, Sean P

    Schizophrenia research

    2023  Volume 257, Page(s) 19–24

    Abstract: Individuals with schizophrenia spectrum disorders (SSD) and a history of experiencing auditory verbal hallucinations (AVH) exhibit poor pitch discrimination relative to those with an SSD but no AVH history. The present study extended this research, ... ...

    Abstract Individuals with schizophrenia spectrum disorders (SSD) and a history of experiencing auditory verbal hallucinations (AVH) exhibit poor pitch discrimination relative to those with an SSD but no AVH history. The present study extended this research, asking if a lifetime history, and the current presence, of AVH exacerbated the pitch discrimination challenges that are seen in SSD. Participants completed a pitch discrimination task, where the tones presented differed in pitch by either 2 %, 5 %, 10 %, 25 % or 50 %. Pitch discrimination accuracy, sensitivity, reaction time (RT) and intra-individual RT variability (IIV) were examined in individuals with SSD and AVHs (AVH+; n = 46), or without AVHs (AVH-; n = 31), and healthy controls (HC; n = 131). Secondary analyses split the AVH+ group into state (i.e., actively experiencing AVH; n = 32) and trait hallucinators (i.e., a history of, but not actively experiencing, AVH; n = 16). Relative to HC, significantly poorer accuracy and sensitivity was detected in individuals with SSD at 2 % and 5 % pitch deviants, and in hallucinators at 10 %; however, no significant differences in accuracy, sensitivity, RT nor IIV were found between AVH+ and AVH- groups. No differences between state and trait hallucinators were observed. A general SSD deficit drove the current findings. The findings may inform future research into the auditory processing capabilities of AVH+ individuals.
    MeSH term(s) Humans ; Schizophrenia/complications ; Pitch Discrimination ; Hallucinations/diagnosis ; Auditory Perception ; Reaction Time
    Language English
    Publishing date 2023-05-24
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 639422-x
    ISSN 1573-2509 ; 0920-9964
    ISSN (online) 1573-2509
    ISSN 0920-9964
    DOI 10.1016/j.schres.2023.05.013
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    Zs.A 2351: Show issues Location:
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    Zs.MO 543: Show issues

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  10. Article ; Online: Intra- and interspecific diversity in a tropical plant clade alter herbivory and ecosystem resilience.

    Grele, Ari / Massad, Tara J / Uckele, Kathryn A / Dyer, Lee A / Antonini, Yasmine / Braga, Laura / Forister, Matthew L / Sulca, Lidia / Kato, Massuo / Lopez, Humberto G / Nascimento, André R / Parchman, Thomas / Simbaña, Wilmer R / Smilanich, Angela M / Stireman, John O / Tepe, Eric J / Walla, Thomas / Richards, Lora A

    eLife

    2024  Volume 12

    Abstract: Declines in biodiversity generated by anthropogenic stressors at both species and population levels can alter emergent processes instrumental to ecosystem function and resilience. As such, understanding the role of biodiversity in ecosystem function and ... ...

    Abstract Declines in biodiversity generated by anthropogenic stressors at both species and population levels can alter emergent processes instrumental to ecosystem function and resilience. As such, understanding the role of biodiversity in ecosystem function and its response to climate perturbation is increasingly important, especially in tropical systems where responses to changes in biodiversity are less predictable and more challenging to assess experimentally. Using large-scale transplant experiments conducted at five neotropical sites, we documented the impacts of changes in intraspecific and interspecific plant richness in the genus
    MeSH term(s) Herbivory ; Animals ; Biodiversity ; Tropical Climate ; Insecta/physiology ; Ecosystem ; Piper/physiology
    Language English
    Publishing date 2024-04-25
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2687154-3
    ISSN 2050-084X ; 2050-084X
    ISSN (online) 2050-084X
    ISSN 2050-084X
    DOI 10.7554/eLife.86988
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