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Article ; Online: The Cby3/ciBAR1 complex positions the annulus along the sperm flagellum during spermiogenesis.

Hoque, Mohammed / Li, Feng-Qian / Weber, William David / Chen, Jun Jie / Kim, Eunice N / Kuo, Pao-Lin / Visconti, Pablo E / Takemaru, Ken-Ichi

The Journal of cell biology

2024 Volume 223, Issue 3

Abstract: Proper compartmentalization of the sperm flagellum is essential for fertility. The annulus is a septin-based ring that demarcates the midpiece (MP) and the principal piece (PP). It is assembled at the flagellar base, migrates caudally, and halts upon ... ...

Abstract	Proper compartmentalization of the sperm flagellum is essential for fertility. The annulus is a septin-based ring that demarcates the midpiece (MP) and the principal piece (PP). It is assembled at the flagellar base, migrates caudally, and halts upon arriving at the PP. However, the mechanisms governing annulus positioning remain unknown. We report that a Chibby3 (Cby3)/Cby1-interacting BAR domain-containing 1 (ciBAR1) complex is required for this process. Ablation of either gene in mice results in male fertility defects, caused by kinked sperm flagella with the annulus mispositioned in the PP. Cby3 and ciBAR1 interact and colocalize to the annulus near the curved membrane invagination at the flagellar pocket. In the absence of Cby3, periannular membranes appear to be deformed, allowing the annulus to migrate over the fibrous sheath into the PP. Collectively, our results suggest that the Cby3/ciBAR1 complex regulates local membrane properties to position the annulus at the MP/PP junction.
MeSH term(s)	Animals ; Male ; Mice ; Cilia ; Cytoskeleton ; Semen ; Sperm Tail ; Spermatogenesis/genetics ; Nuclear Proteins/genetics ; Carrier Proteins/genetics
Chemical Substances	Nuclear Proteins ; Carrier Proteins
Language	English
Publishing date	2024-01-10
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	218154-x
ISSN	1540-8140 ; 0021-9525
ISSN (online)	1540-8140
ISSN	0021-9525
DOI	10.1083/jcb.202307147
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Structural features of a minimal intact methyltransferase of a type I restriction-modification system.

Seo, Pil-Won / Hofmann, Andreas / Kim, Jun-Ha / Hwangbo, Seung-A / Kim, Jun-Hong / Kim, Ji-Won / Huynh, Thi Yen Ly / Choy, Hyon E / Kim, Soo-Jung / Lee, Jimin / Lee, Jie-Oh / Jin, Kyeong Sik / Park, Suk-Youl / Kim, Jeong-Sun

International journal of biological macromolecules

2022 Volume 208, Page(s) 381–389

Abstract: Type I restriction-modification enzymes are oligomeric proteins composed of methylation (M), DNA sequence-recognition (S), and restriction (R) subunits. The different bipartite DNA sequences of 2-4 consecutive bases are recognized by two discerned target ...

Abstract	Type I restriction-modification enzymes are oligomeric proteins composed of methylation (M), DNA sequence-recognition (S), and restriction (R) subunits. The different bipartite DNA sequences of 2-4 consecutive bases are recognized by two discerned target recognition domains (TRDs) located at the two-helix bundle of the two conserved regions (CRs). Two M-subunits and a single S-subunit form an oligomeric protein that functions as a methyltransferase (M
MeSH term(s)	Amino Acid Sequence ; DNA/chemistry ; DNA Restriction-Modification Enzymes/chemistry ; DNA Restriction-Modification Enzymes/genetics ; DNA Restriction-Modification Enzymes/metabolism ; Methylation ; Methyltransferases/chemistry
Chemical Substances	DNA Restriction-Modification Enzymes ; DNA (9007-49-2) ; Methyltransferases (EC 2.1.1.-)
Language	English
Publishing date	2022-03-23
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2022.03.115
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Article ; Online: Blood biomarkers for the differentiation between central and peripheral vertigo in the emergency department: a systematic review and meta-analysis.

Klokman, Vincent W / Koningstein, Fiona N / Dors, Job W W / Sanders, Marieke S / Koning, Sam W / de Kleijn, Dominique P V / Jie, Kim E

Academic emergency medicine : official journal of the Society for Academic Emergency Medicine

2024 Volume 31, Issue 4, Page(s) 371–385

Abstract: Background/introduction: In patients with acute vestibular syndrome (AVS), differentiating between stroke and nonstroke causes is challenging in the emergency department (ED). Correct diagnosis of vertigo etiology is essential for early optimum ... ...

Abstract	Background/introduction: In patients with acute vestibular syndrome (AVS), differentiating between stroke and nonstroke causes is challenging in the emergency department (ED). Correct diagnosis of vertigo etiology is essential for early optimum treatment and disposition. Objectives: The aim of this systematic review and meta-analysis was to summarize the published evidence on the potential of blood biomarkers in the diagnosis and differentiation of peripheral from central causes of AVS. Methods: A literature search was conducted for studies published until January 1, 2023, in PubMed, Ovid Medline, and EMBASE databases analyzing biomarkers for the differentiation between central and peripheral AVS. The Quality Assessment of Diagnostic Accuracy Studies questionnaire 2 was used for quality assessment. Pooled standardized mean difference and 95% confidence intervals were calculated if a biomarker was reported in two or more studies. Heterogeneity among included studies was investigated using the I Results: A total of 17 studies with 859 central and 4844 peripheral causes of acute dizziness or vertigo, and analysis of 61 biomarkers were included. The general laboratory markers creatinine, blood urea nitrogen, albumin, C-reactive protein, glucose, HbA1c, leukocyte counts, and neutrophil counts and the brain-derived biomarkers copeptin, S100 calcium-binding protein β (S100β), and neuron-specific enolase (NSE) significantly differentiated central from peripheral causes of AVS. Conclusions: This systematic review and meta-analysis highlights the potential of generalized inflammatory markers and brain-specific blood protein markers of NSE and S100β as diagnostic biomarkers for central from peripheral differentiation in AVS. These results, as a complement to clinical characteristics, provide guidance for future large-scale diagnostic research, in this challenging ED patient population.
MeSH term(s)	Humans ; Vertigo/diagnosis ; Vertigo/etiology ; Vestibular Diseases/complications ; Stroke/diagnosis ; Biomarkers ; Emergency Service, Hospital ; Dizziness
Chemical Substances	Biomarkers
Language	English
Publishing date	2024-02-25
Publishing country	United States
Document type	Meta-Analysis ; Systematic Review ; Journal Article ; Review
ZDB-ID	1329813-6
ISSN	1553-2712 ; 1069-6563
ISSN (online)	1553-2712
ISSN	1069-6563
DOI	10.1111/acem.14864
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 4479: Show issues

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Article ; Online: Structural features of a minimal intact methyltransferase of a type I restriction-modification system

Seo, Pil-Won / Hofmann, Andreas / Kim, Jun-Ha / Hwangbo, Seung-A / Kim, Jun-Hong / Kim, Ji-Won / Huynh, Thi Yen Ly / Choy, Hyon E. / Kim, Soo-Jung / Lee, Jimin / Lee, Jie-Oh / Jin, Kyeong Sik / Park, Suk-Youl / Kim, Jeong-Sun

2022

Keywords	Text ; ddc:660 ; Methyltransferase ; Ocr ; Type I restriction-modification system
Language	English
Publishing date	2022-03-23
Publishing country	de
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Patient Education Practices and Preferences of Radiation Oncologists and Interprofessional Radiation Therapy Care Teams: A Mixed-Methods Study Exploring Strategies for Effective Patient Education Delivery.

Chen, Jie Jane / Brown, Anna M / Garda, Allison E / Kim, Ellen / McAvoy, Sarah A / Perni, Subha / Rooney, Michael K / Shiue, Kevin / Tonning, Kristi L / Warren, Laura E / Golden, Daniel W / Croke, Jennifer M

International journal of radiation oncology, biology, physics

2024

Abstract: Purpose: Patients' understanding of radiation therapy (RT) and data regarding optimal approaches to patient education (PE) within radiation oncology (RO) are limited. We aimed to evaluate PE practices of radiation oncologists and interprofessional RT ... ...

Abstract	Purpose: Patients' understanding of radiation therapy (RT) and data regarding optimal approaches to patient education (PE) within radiation oncology (RO) are limited. We aimed to evaluate PE practices of radiation oncologists and interprofessional RT care team members to inform recommendations for delivering inclusive and accessible PE. Methods and materials: An anonymous survey was administered to all Radiation Oncology Education Collaborative Study Group members (10/5/22-11/23/22). Respondent demographics, individual practices/preferences, and institutional practices were collected. Qualitative items explored strategies, challenges, and desired resources for PE. Descriptive statistics summarized survey responses. The Fisher exact test compared PE practices by respondent role and PE timing. Thematic analysis was used for qualitative responses. Results: One hundred thirteen Radiation Oncology Education Collaborative Study Group members completed the survey (28.2% response rate); RO attendings comprised 68.1% of respondents. Most practiced in an academic setting (85.8%) in North America (80.5%). Institution-specific materials were the most common PE resource used by radiation oncologists (67.6%). Almost half (40.2%) reported that their PE practices differed based on clinical encounter type, with paper handouts commonly used for in-person and multimedia for telehealth visits. Only 57.7% reported access to non-English PE materials. PE practices among radiation oncologists differed according to RT clinical workflow timing (consultation versus simulation versus first RT, respectively): one-on-one teaching: 88.5% versus 49.4% versus 56.3%, P < .01, and paper handouts: 69.0% versus 28.7% versus 16.1%, P < .01. Identified challenges for PE delivery included limited time, administrative barriers to the development or implementation of new materials or practices, and a lack of customized resources for tailored PE. Effective strategies for PE included utilization of visual diagrams, multimedia, and innovative education techniques to personalize PE delivery/resources for a diverse patient population, as well as fostering interprofessional collaboration to reinforce educational content. Conclusions: Radiation oncologists and interprofessional RO team members engage in PE, with most using institution-specific materials often available only in English. PE practices differ according to clinical encounter type and RT workflow timing. Increased adoption of multimedia materials and partnerships with patients to tailor PE resources are needed to foster high-quality, patient-centered PE delivery.
Language	English
Publishing date	2024-03-02
Publishing country	United States
Document type	Journal Article
ZDB-ID	197614-x
ISSN	1879-355X ; 0360-3016
ISSN (online)	1879-355X
ISSN	0360-3016
DOI	10.1016/j.ijrobp.2024.02.023
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Zs.A 1218: Show issues

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Article ; Online: Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows.

Sibert, Bryan S / Kim, Joseph Y / Yang, Jie E / Wright, Elizabeth R

Journal of visualized experiments : JoVE

2021 , Issue 175

Abstract: Whole-cell cryo-electron tomography (cryo-ET) is a powerful technology that is used to produce nanometer-level resolution structures of macromolecules present in the cellular context and preserved in a near-native frozen-hydrated state. However, there ... ...

Abstract	Whole-cell cryo-electron tomography (cryo-ET) is a powerful technology that is used to produce nanometer-level resolution structures of macromolecules present in the cellular context and preserved in a near-native frozen-hydrated state. However, there are challenges associated with culturing and/or adhering cells onto TEM grids in a manner that is suitable for tomography while retaining the cells in their physiological state. Here, a detailed step-by-step protocol is presented on the use of micropatterning to direct and promote eukaryotic cell growth on TEM grids. During micropatterning, cell growth is directed by depositing extra-cellular matrix (ECM) proteins within specified patterns and positions on the foil of the TEM grid while the other areas remain coated with an anti-fouling layer. Flexibility in the choice of surface coating and pattern design makes micropatterning broadly applicable for a wide range of cell types. Micropatterning is useful for studies of structures within individual cells as well as more complex experimental systems such as host-pathogen interactions or differentiated multi-cellular communities. Micropatterning may also be integrated into many downstream whole-cell cryo-ET workflows, including correlative light and electron microscopy (cryo-CLEM) and focused-ion beam milling (cryo-FIB).
MeSH term(s)	Cryoelectron Microscopy ; Electron Microscope Tomography ; Freezing ; HeLa Cells ; Humans ; Microscopy, Electron ; Workflow
Language	English
Publishing date	2021-09-13
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Video-Audio Media
ZDB-ID	2259946-0
ISSN	1940-087X ; 1940-087X
ISSN (online)	1940-087X
ISSN	1940-087X
DOI	10.3791/62992
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: In reply.

Berg, Hidde Ten / van Bakel, Bram / van de Wouw, Lieke / Jie, Kim E / Schipper, Anoeska / Jansen, Henry / O'Connor, Rory D / van Ginneken, Bram / Kurstjens, Steef

Annals of emergency medicine

2023 Volume 83, Issue 3, Page(s) 287–288

Language	English
Publishing date	2023-12-13
Publishing country	United States
Document type	Letter
ZDB-ID	603080-4
ISSN	1097-6760 ; 0196-0644
ISSN (online)	1097-6760
ISSN	0196-0644
DOI	10.1016/j.annemergmed.2023.10.007
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Zs.A 1344: Show issues

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Article ; Online: ChatGPT and Generating a Differential Diagnosis Early in an Emergency Department Presentation.

Berg, Hidde Ten / van Bakel, Bram / van de Wouw, Lieke / Jie, Kim E / Schipper, Anoeska / Jansen, Henry / O'Connor, Rory D / van Ginneken, Bram / Kurstjens, Steef

Annals of emergency medicine

2023 Volume 83, Issue 1, Page(s) 83–86

MeSH term(s)	Humans ; Diagnosis, Differential ; Artificial Intelligence ; Emergency Service, Hospital
Language	English
Publishing date	2023-09-09
Publishing country	United States
Document type	Journal Article
ZDB-ID	603080-4
ISSN	1097-6760 ; 0196-0644
ISSN (online)	1097-6760
ISSN	0196-0644
DOI	10.1016/j.annemergmed.2023.08.003
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Zs.A 1344: Show issues

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Article: Handling difficult cryo-ET samples: A study with primary neurons from

Kim, Joseph Y / Yang, Jie E / Mitchell, Josephine W / English, Lauren A / Yang, Sihui Z / Tenpas, Tanner / Dent, Erik W / Wildonger, Jill / Wright, Elizabeth R

bioRxiv : the preprint server for biology

2023

Abstract: Cellular neurobiology has benefited from recent advances in the field of cryo-electron tomography (cryo-ET). Numerous structural and ultrastructural insights have been obtained from plunge-frozen primary neurons cultured on electron microscopy grids. ... ...

Abstract	Cellular neurobiology has benefited from recent advances in the field of cryo-electron tomography (cryo-ET). Numerous structural and ultrastructural insights have been obtained from plunge-frozen primary neurons cultured on electron microscopy grids. With most primary neurons been derived from rodent sources, we sought to expand the breadth of sample availability by using primary neurons derived from 3
Language	English
Publishing date	2023-07-11
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.07.10.548468
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: In reply.

Jie, Kim E / Hammacher, Eric R

Annals of emergency medicine

2015 Volume 65, Issue 2, Page(s) 233–234

MeSH term(s)	Arm Injuries/diagnosis ; Elbow Joint/injuries ; Female ; Humans ; Male ; Physical Examination/methods
Language	English
Publishing date	2015-02
Publishing country	United States
Document type	Comment ; Letter
ZDB-ID	603080-4
ISSN	1097-6760 ; 0196-0644
ISSN (online)	1097-6760
ISSN	0196-0644
DOI	10.1016/j.annemergmed.2014.10.002
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