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  1. Article ; Online: Transcription activation in

    Busby, Stephen J W / Browning, Douglas F

    EcoSal Plus

    2024  , Page(s) eesp00392020

    Abstract: Promoter-specific activation of transcript initiation provides an important regulatory device ... ...

    Abstract Promoter-specific activation of transcript initiation provides an important regulatory device in
    Language English
    Publishing date 2024-02-12
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 2324-6200
    ISSN (online) 2324-6200
    DOI 10.1128/ecosalplus.esp-0039-2020
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  2. Article ; Online: Laboratory strains of

    Browning, Douglas F / Hobman, Jon L / Busby, Stephen J W

    Microbial genomics

    2023  Volume 9, Issue 2

    Abstract: Escherichia ... ...

    Abstract Escherichia coli
    MeSH term(s) Escherichia coli/genetics ; Escherichia coli K12/genetics ; Bacteriophage lambda ; Base Sequence
    Language English
    Publishing date 2023-02-06
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2835258-0
    ISSN 2057-5858 ; 2057-5858
    ISSN (online) 2057-5858
    ISSN 2057-5858
    DOI 10.1099/mgen.0.000922
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  3. Article ; Online: Preliminary Evidence for Dementia Collaborative Coaching.

    Douglas, Natalie F / Browning, Susan / Claypool, Kathy

    American journal of speech-language pathology

    2023  Volume 32, Issue 5, Page(s) 2146–2157

    Abstract: Purpose: The primary purpose of this study was to obtain preliminary evidence for a communication coaching intervention, Dementia Collaborative Coaching. The secondary aim of this study was to assess the acceptability, appropriateness, and feasibility ... ...

    Abstract Purpose: The primary purpose of this study was to obtain preliminary evidence for a communication coaching intervention, Dementia Collaborative Coaching. The secondary aim of this study was to assess the acceptability, appropriateness, and feasibility of the intervention according to routine care providers.
    Method: In a pre-/posttest design, speech-language pathologists (SLPs) delivered Dementia Collaborative Coaching to certified nursing assistants (CNAs) and people living with dementia (PLWD) in six different skilled nursing facilities over a period of 6 weeks. A self-perceived knowledge and efficacy measure regarding the use of external memory aids to support communication in PLWD was administered to CNA and SLP participants. The Cohen-Mansfield Agitation Inventory was administered to PLWD participants. The Acceptability of Intervention Measure, Intervention Appropriateness Measure, and Feasibility of Intervention Measure were administered post-intervention.
    Results: For CNAs, self-perceived knowledge and efficacy increased from pre-intervention (
    Conclusions: Dementia Collaborative Coaching showed preliminary positive outcomes for CNAs and PLWD. The intervention was acceptable, appropriate, and feasible for routine providers and warrants further study.
    MeSH term(s) Humans ; Mentoring ; Nursing Assistants ; Certification ; Communication ; Dementia/diagnosis ; Dementia/therapy
    Language English
    Publishing date 2023-07-12
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1154406-5
    ISSN 1558-9110 ; 1058-0360
    ISSN (online) 1558-9110
    ISSN 1058-0360
    DOI 10.1044/2023_AJSLP-22-00367
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3716: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  4. Article ; Online: The Small DdrR Protein Directly Interacts with the UmuDAb Regulator of the Mutagenic DNA Damage Response in Acinetobacter baumannii.

    Pavlin, Anja / Bajc, Gregor / Fornelos, Nadine / Browning, Douglas F / Butala, Matej

    Journal of bacteriology

    2022  Volume 204, Issue 3, Page(s) e0060121

    Abstract: Acinetobacter baumannii poses a great threat in health care settings worldwide, with clinical isolates displaying an ever-evolving multidrug resistance. In strains of A. baumannii, expression of multiple error-prone polymerase genes are corepressed by ... ...

    Abstract Acinetobacter baumannii poses a great threat in health care settings worldwide, with clinical isolates displaying an ever-evolving multidrug resistance. In strains of A. baumannii, expression of multiple error-prone polymerase genes are corepressed by UmuDAb, a member of the LexA superfamily, and a small protein, DdrR. It is currently unknown how DdrR establishes this repression. Here, we used surface plasmon resonance spectrometry to show that DdrR formed a stable complex with the UmuDAb regulator. Our results indicated that the carboxy-terminal dimerization domain of UmuDAb formed the interaction interface with DdrR. Our
    MeSH term(s) Acinetobacter baumannii/genetics ; Acinetobacter baumannii/metabolism ; Bacterial Proteins/metabolism ; DNA Damage ; Gene Expression Regulation, Bacterial ; Mutagens ; Transcription Factors/metabolism
    Chemical Substances Bacterial Proteins ; Mutagens ; Transcription Factors
    Language English
    Publishing date 2022-02-22
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2968-3
    ISSN 1098-5530 ; 0021-9193
    ISSN (online) 1098-5530
    ISSN 0021-9193
    DOI 10.1128/jb.00601-21
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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.50: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Novel organisation and regulation of the

    Alhammadi, Munirah M / Godfrey, Rita E / Ingram, Joseph O / Singh, Gurdamanjit / Bathurst, Camilla L / Busby, Stephen J W / Browning, Douglas F

    Virulence

    2022  Volume 13, Issue 1, Page(s) 1393–1406

    Abstract: The serine protease autotransporters of ... ...

    Abstract The serine protease autotransporters of the
    MeSH term(s) Carbon/metabolism ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Escherichia coli Infections/microbiology ; Escherichia coli Proteins/genetics ; Escherichia coli Proteins/metabolism ; Mucins/metabolism ; Promoter Regions, Genetic ; Serine Endopeptidases/genetics ; Serine Endopeptidases/metabolism ; Uropathogenic Escherichia coli/genetics ; Uropathogenic Escherichia coli/metabolism ; Virulence Factors/genetics ; Virulence Factors/metabolism
    Chemical Substances Escherichia coli Proteins ; Mucins ; Virulence Factors ; Carbon (7440-44-0) ; Pic protein, E coli (EC 3.4.21.-) ; Serine Endopeptidases (EC 3.4.21.-)
    Language English
    Publishing date 2022-09-21
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2657572-3
    ISSN 2150-5608 ; 2150-5594
    ISSN (online) 2150-5608
    ISSN 2150-5594
    DOI 10.1080/21505594.2022.2111754
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  6. Article ; Online: New vectors for urea-inducible recombinant protein production.

    Hothersall, Joanne / Osgerby, Alexander / Godfrey, Rita E / Overton, Tim W / Busby, Stephen J W / Browning, Douglas F

    New biotechnology

    2022  Volume 72, Page(s) 89–96

    Abstract: We have developed a novel urea-inducible recombinant protein production system by exploiting the Proteus mirabilis urease ureR-ureD promoter region and the ureR AraC-family transcriptional regulator. Experiments using the expression of β-galactosidase ... ...

    Abstract We have developed a novel urea-inducible recombinant protein production system by exploiting the Proteus mirabilis urease ureR-ureD promoter region and the ureR AraC-family transcriptional regulator. Experiments using the expression of β-galactosidase and green fluorescent protein (GFP) showed that promoter activity is tightly regulated and that varying the concentration of urea can give up to 100-fold induction. Production of proteins of biopharmaceutical interest has been demonstrated, including human growth hormone (hGH), a single chain antibody fragment (scFv) against interleukin-1β and a potential Neisserial vaccine candidate (BamA
    Language English
    Publishing date 2022-10-20
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2400836-9
    ISSN 1876-4347 ; 1876-4347
    ISSN (online) 1876-4347
    ISSN 1876-4347
    DOI 10.1016/j.nbt.2022.10.003
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  7. Article ; Online: Bacterial Transcription Factors: Regulation by Pick "N" Mix.

    Browning, Douglas F / Butala, Matej / Busby, Stephen J W

    Journal of molecular biology

    2019  Volume 431, Issue 20, Page(s) 4067–4077

    Abstract: Transcription in most bacteria is tightly regulated in order to facilitate bacterial adaptation to different environments, and transcription factors play a key role in this. Here we give a brief overview of the essential features of bacterial ... ...

    Abstract Transcription in most bacteria is tightly regulated in order to facilitate bacterial adaptation to different environments, and transcription factors play a key role in this. Here we give a brief overview of the essential features of bacterial transcription factors and how they affect transcript initiation at target promoters. We focus on complex promoters that are regulated by combinations of activators and repressors, combinations of repressors only, or combinations of activators. At some promoters, transcript initiation is regulated by nucleoid-associated proteins, which often work together with transcription factors. We argue that the distinction between nucleoid-associated proteins and transcription factors is blurred and that they likely share common origins.
    MeSH term(s) Bacteria/genetics ; Bacteria/metabolism ; Bacterial Proteins/metabolism ; Gene Expression Regulation, Bacterial ; Promoter Regions, Genetic ; Transcription Factors/metabolism ; Transcription Initiation, Genetic
    Chemical Substances Bacterial Proteins ; Transcription Factors
    Language English
    Publishing date 2019-04-16
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 80229-3
    ISSN 1089-8638 ; 0022-2836
    ISSN (online) 1089-8638
    ISSN 0022-2836
    DOI 10.1016/j.jmb.2019.04.011
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 867: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article ; Online: Heterologous Expression of Membrane Proteins in E. coli.

    Depping, Peer / Román Lara, María Monserrat / Kesidis, Athanasios / Bill, Roslyn M / Rothnie, Alice J / Browning, Douglas F / Goddard, Alan D

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2507, Page(s) 59–78

    Abstract: Over the decades, the bacterium Escherichia coli (E. coli) has become the cornerstone of recombinant protein production, used for heterologous synthesis of a variety of membrane proteins. Due to its rapid growth to high densities in cheap media, and its ... ...

    Abstract Over the decades, the bacterium Escherichia coli (E. coli) has become the cornerstone of recombinant protein production, used for heterologous synthesis of a variety of membrane proteins. Due to its rapid growth to high densities in cheap media, and its ease of manipulation and handling, E. coli is an excellent host cell for a range of membrane protein targets. Furthermore, its genetic tractability allows for a variety of gene constructs to be screened for optimal expression conditions, resulting in relatively high yields of membrane protein in a short amount of time. Here, we describe the general workflow for the production of membrane proteins in E. coli. The protocols we provide show how the gene of interest is modified, transferred to an expression vector and host, and how membrane protein yields can be optimized and analyzed. The examples we illustrate are well suited for scientists who are starting their journey into the world of membrane protein production.
    MeSH term(s) Escherichia coli/genetics ; Escherichia coli/metabolism ; Escherichia coli Proteins/metabolism ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Protein Transport ; Recombinant Proteins/metabolism
    Chemical Substances Escherichia coli Proteins ; Membrane Proteins ; Recombinant Proteins
    Language English
    Publishing date 2022-06-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-2368-8_4
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  9. Article ; Online: Control of resistance against bacteriophage killing by a metabolic regulator in meningitis-associated <i>Escherichia coli</i>.

    Connolly, James P R / Turner, Natasha C A / Serrano, Ester / Rimbi, Patricia T / Browning, Douglas F / O'Boyle, Nicky / Roe, Andrew J

    Proceedings of the National Academy of Sciences of the United States of America

    2022  Volume 119, Issue 45, Page(s) e2210299119

    Abstract: Ecologically beneficial traits in bacteria are encoded by intrinsic and horizontally acquired genes. However, such traits are not universal, and the highly mosaic nature of bacterial genomes requires control at the transcriptional level to drive these ... ...

    Abstract Ecologically beneficial traits in bacteria are encoded by intrinsic and horizontally acquired genes. However, such traits are not universal, and the highly mosaic nature of bacterial genomes requires control at the transcriptional level to drive these processes. It has emerged that regulatory flexibility is widespread in the Escherichia coli species, whereby preexisting transcription factors can acquire new and unrelated roles in regulating beneficial traits. DsdC is the regulator of D-serine tolerance in E. coli, is essential for D-serine catabolism, and is often encoded by two copies in neonatal meningitis-associated E. coli (NMEC). Here, we reveal that DsdC is a global regulator of transcription in NMEC and does not require D-serine for the control of novel beneficial traits. We show that DsdC binds the chromosome in an unusual manner, with many binding sites arranged in clusters spanning entire operons and within gene coding sequences, such as neuO. Importantly, we identify neuO as the most significantly down-regulated gene in a strain deleted for both dsdC copies, in both the presence and absence of D-serine. NeuO is prophage encoded in several NMEC K1 isolates and mediates capsule O-acetylation but has no effect on attachment to or invasion of human brain endothelial cells. Instead, we demonstrate that NeuO provides resistance against K1 bacteriophage attack and that this critical function is regulated by DsdC. This work highlights how a horizontally acquired enzyme that functions in cell-surface modulation can be controlled by an intrinsic regulator to provide a key ecological benefit to an E. coli pathotype.
    MeSH term(s) Infant, Newborn ; Humans ; Escherichia coli/metabolism ; Escherichia coli Proteins/metabolism ; Bacteriophages/metabolism ; Endothelial Cells/metabolism ; Serine/metabolism
    Chemical Substances Escherichia coli Proteins ; Serine (452VLY9402)
    Language English
    Publishing date 2022-11-02
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2210299119
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1148: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  10. Article: Antimicrobial Resistance and Comparative Genome Analysis of

    Abdelwahab, Radwa / Alhammadi, Munirah M / Hassan, Ehsan A / Ahmed, Entsar H / Abu-Faddan, Nagla H / Daef, Enas A / Busby, Stephen J W / Browning, Douglas F

    Microorganisms

    2021  Volume 9, Issue 9

    Abstract: Klebsiella ... ...

    Abstract Klebsiella pneumoniae
    Language English
    Publishing date 2021-09-05
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms9091880
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