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  1. Article ; Online: Frequency of side effects of sofosbuvir and daclatsavir in patients of chronic Hepatitis C

    khawaja tahir maqbool / Sehrish Rashid / Arslan Shehzad / Sidra Rafique

    Journal of Rawalpindi Medical College, Vol 23, Iss

    2019  Volume 3

    Abstract: i am submitting undertaking ... ...

    Abstract i am submitting undertaking form
    Keywords Medicine ; R
    Language English
    Publishing date 2019-09-01T00:00:00Z
    Publisher Rawalpindi Medical University
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Screening of multiclass pesticide residues in honey by SPE-GC/MSD: a pilot study.

    Rafique, Nazia / Nazir, Sehrish / Akram, Sumaira / Ahad, Karam / Gohar, Afshan / Abbasi, Surriya Tariq / Ahmed, Ijaz / Rafique, Khalid

    Environmental monitoring and assessment

    2018  Volume 190, Issue 11, Page(s) 666

    Abstract: Analytical method for the monitoring of residues of multiclass pesticides (variable chemical structure and chromatographic behavior) in honey has been optimized and in-house validated in the present study. Chemical confirmation of 35 selected pesticides ( ...

    Abstract Analytical method for the monitoring of residues of multiclass pesticides (variable chemical structure and chromatographic behavior) in honey has been optimized and in-house validated in the present study. Chemical confirmation of 35 selected pesticides (in-hive-treated pesticides and pesticides applied for agricultural practices in vicinity of apiaries) has been successfully achieved with the acetonitrile extraction/partitioning and cleanup by modified US EPA solid-phase extraction (SPE) protocol following analysis on the GC/MS DRS Pesticide Screener. The applied extraction procedure has given acceptable recoveries with an associated precision (RSD) for selected pesticides within the range as suggested by SANTE at MQL of 10 μg kg
    MeSH term(s) Environmental Monitoring/methods ; Gas Chromatography-Mass Spectrometry/methods ; Honey/analysis ; Pakistan ; Pesticide Residues/analysis ; Pesticides/analysis ; Pilot Projects ; Solid Phase Extraction
    Chemical Substances Pesticide Residues ; Pesticides
    Language English
    Publishing date 2018-10-22
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 782621-7
    ISSN 1573-2959 ; 0167-6369
    ISSN (online) 1573-2959
    ISSN 0167-6369
    DOI 10.1007/s10661-018-7041-4
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  3. Article: Screening of multiclass pesticide residues in honey by SPE-GC/MSD: a pilot study

    Rafique, Nazia / Sehrish Nazir / Sumaira Akram / Karam Ahad / Afshan Gohar / Surriya Tariq Abbasi / Ijaz Ahmed / Khalid Rafique

    Environmental monitoring and assessment. 2018 Nov., v. 190, no. 11

    2018  

    Abstract: Analytical method for the monitoring of residues of multiclass pesticides (variable chemical structure and chromatographic behavior) in honey has been optimized and in-house validated in the present study. Chemical confirmation of 35 selected pesticides ( ...

    Abstract Analytical method for the monitoring of residues of multiclass pesticides (variable chemical structure and chromatographic behavior) in honey has been optimized and in-house validated in the present study. Chemical confirmation of 35 selected pesticides (in-hive-treated pesticides and pesticides applied for agricultural practices in vicinity of apiaries) has been successfully achieved with the acetonitrile extraction/partitioning and cleanup by modified US EPA solid-phase extraction (SPE) protocol following analysis on the GC/MS DRS Pesticide Screener. The applied extraction procedure has given acceptable recoveries with an associated precision (RSD) for selected pesticides within the range as suggested by SANTE at MQL of 10 μg kg⁻¹. Potential matrix effect for selected analytes was calculated by using honey from five different floral sources. The optimized method was used to determine levels of pesticide residues in honey samples randomly collected from 26 different apiaries in Pakistan. Residues of nine selected pesticide (dichlorvos, mevinphos, ethalfluralin, trifluralin, lindane, chlorpyrifos–methyl, dieldrin, profenofos, 4,4-DDE) were frequently detected in the ranges of 3–48.8 μg kg⁻¹ in 26.9% of analyzed samples (n = 26) and 15.3% of the studied samples exceeded maximum residue limits (MRLs). In-hive-treated acaricides, i.e., coumaphos, tau-fluvalinate, and malathion, were not detected in any of the analyzed honey samples.
    Keywords DDE (pesticide) ; United States Environmental Protection Agency ; acetonitrile ; apiaries ; chemical species ; chemical structure ; chlorpyrifos-methyl ; chromatography ; coumaphos ; dichlorvos ; dieldrin ; ethalfluralin ; fluvalinate ; honey ; lindane ; malathion ; maximum residue limits ; mevinphos ; monitoring ; pesticide residues ; profenofos ; protocols ; screening ; solid phase extraction ; trifluralin ; Pakistan
    Language English
    Dates of publication 2018-11
    Size p. 666.
    Publishing place Springer International Publishing
    Document type Article
    ZDB-ID 782621-7
    ISSN 1573-2959 ; 0167-6369
    ISSN (online) 1573-2959
    ISSN 0167-6369
    DOI 10.1007/s10661-018-7041-4
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    In stock of ZB MED Bonn / Germany

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  4. Article ; Online: Estrogen-induced chromatin decondensation and nuclear re-organization linked to regional epigenetic regulation in breast cancer.

    Rafique, Sehrish / Thomas, Jeremy S / Sproul, Duncan / Bickmore, Wendy A

    Genome biology

    2015  Volume 16, Page(s) 145

    Abstract: Background: Epigenetic changes are being increasingly recognized as a prominent feature of cancer. This occurs not only at individual genes, but also over larger chromosomal domains. To investigate this, we set out to identify large chromosomal domains ... ...

    Abstract Background: Epigenetic changes are being increasingly recognized as a prominent feature of cancer. This occurs not only at individual genes, but also over larger chromosomal domains. To investigate this, we set out to identify large chromosomal domains of epigenetic dysregulation in breast cancers.
    Results: We identify large regions of coordinate down-regulation of gene expression, and other regions of coordinate activation, in breast cancers and show that these regions are linked to tumor subtype. In particular we show that a group of coordinately regulated regions are expressed in luminal, estrogen-receptor positive breast tumors and cell lines. For one of these regions of coordinate gene activation, we show that regional epigenetic regulation is accompanied by visible unfolding of large-scale chromatin structure and a repositioning of the region within the nucleus. In MCF7 cells, we show that this depends on the presence of estrogen.
    Conclusions: Our data suggest that the liganded estrogen receptor is linked to long-range changes in higher-order chromatin organization and epigenetic dysregulation in cancer. This may suggest that as well as drugs targeting histone modifications, it will be valuable to investigate the inhibition of protein complexes involved in chromatin folding in cancer cells.
    MeSH term(s) Breast Neoplasms/genetics ; Breast Neoplasms/metabolism ; Cell Line, Tumor ; Cell Nucleus/genetics ; Chromatin/chemistry ; Epigenesis, Genetic ; Estrogens/physiology ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; MCF-7 Cells ; Receptors, Estrogen/metabolism ; Transcription, Genetic
    Chemical Substances Chromatin ; Estrogens ; Receptors, Estrogen
    Language English
    Publishing date 2015-08-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2040529-7
    ISSN 1474-760X ; 1474-760X
    ISSN (online) 1474-760X
    ISSN 1474-760X
    DOI 10.1186/s13059-015-0719-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Estrogen-induced chromatin decondensation and nuclear re-organization linked to regional epigenetic regulation in breast cancer

    Rafique, Sehrish / Thomas, Jeremy S / Sproul, Duncan / Bickmore, Wendy A

    Genome biology. 2015 Dec., v. 16, no. 1

    2015  

    Abstract: BACKGROUND: Epigenetic changes are being increasingly recognized as a prominent feature of cancer. This occurs not only at individual genes, but also over larger chromosomal domains. To investigate this, we set out to identify large chromosomal domains ... ...

    Abstract BACKGROUND: Epigenetic changes are being increasingly recognized as a prominent feature of cancer. This occurs not only at individual genes, but also over larger chromosomal domains. To investigate this, we set out to identify large chromosomal domains of epigenetic dysregulation in breast cancers. RESULTS: We identify large regions of coordinate down-regulation of gene expression, and other regions of coordinate activation, in breast cancers and show that these regions are linked to tumor subtype. In particular we show that a group of coordinately regulated regions are expressed in luminal, estrogen-receptor positive breast tumors and cell lines. For one of these regions of coordinate gene activation, we show that regional epigenetic regulation is accompanied by visible unfolding of large-scale chromatin structure and a repositioning of the region within the nucleus. In MCF7 cells, we show that this depends on the presence of estrogen. CONCLUSIONS: Our data suggest that the liganded estrogen receptor is linked to long-range changes in higher-order chromatin organization and epigenetic dysregulation in cancer. This may suggest that as well as drugs targeting histone modifications, it will be valuable to investigate the inhibition of protein complexes involved in chromatin folding in cancer cells.
    Keywords breast neoplasms ; chromatin ; drugs ; epigenetics ; estrogen receptors ; gene activation ; genes ; histones ; neoplasm cells
    Language English
    Dates of publication 2015-12
    Size p. 145.
    Publishing place BioMed Central
    Document type Article
    ZDB-ID 2040529-7
    ISSN 1474-760X ; 1465-6906
    ISSN (online) 1474-760X
    ISSN 1465-6906
    DOI 10.1186/s13059-015-0719-9
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  6. Article ; Online: Activation of estrogen-responsive genes does not require their nuclear co-localization.

    Kocanova, Silvia / Kerr, Elizabeth A / Rafique, Sehrish / Boyle, Shelagh / Katz, Elad / Caze-Subra, Stephanie / Bickmore, Wendy A / Bystricky, Kerstin

    PLoS genetics

    2010  Volume 6, Issue 4, Page(s) e1000922

    Abstract: The spatial organization of the genome in the nucleus plays a role in the regulation of gene expression. Whether co-regulated genes are subject to coordinated repositioning to a shared nuclear space is a matter of considerable interest and debate. We ... ...

    Abstract The spatial organization of the genome in the nucleus plays a role in the regulation of gene expression. Whether co-regulated genes are subject to coordinated repositioning to a shared nuclear space is a matter of considerable interest and debate. We investigated the nuclear organization of estrogen receptor alpha (ERalpha) target genes in human breast epithelial and cancer cell lines, before and after transcriptional activation induced with estradiol. We find that, contrary to another report, the ERalpha target genes TFF1 and GREB1 are distributed in the nucleoplasm with no particular relationship to each other. The nuclear separation between these genes, as well as between the ERalpha target genes PGR and CTSD, was unchanged by hormone addition and transcriptional activation with no evidence for co-localization between alleles. Similarly, while the volume occupied by the chromosomes increased, the relative nuclear position of the respective chromosome territories was unaffected by hormone addition. Our results demonstrate that estradiol-induced ERalpha target genes are not required to co-localize in the nucleus.
    MeSH term(s) Cell Line, Tumor ; Cell Nucleus/metabolism ; Epithelial Cells/metabolism ; Estrogen Receptor alpha/genetics ; Estrogen Receptor alpha/metabolism ; Estrogens/pharmacology ; Female ; Humans ; Neoplasm Proteins/genetics ; Neoplasm Proteins/metabolism ; Trefoil Factor-1 ; Tumor Suppressor Proteins/genetics ; Tumor Suppressor Proteins/metabolism
    Chemical Substances Estrogen Receptor alpha ; Estrogens ; GREB1 protein, human ; Neoplasm Proteins ; TFF1 protein, human ; Trefoil Factor-1 ; Tumor Suppressor Proteins
    Language English
    Publishing date 2010-04-22
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2186725-2
    ISSN 1553-7404 ; 1553-7390
    ISSN (online) 1553-7404
    ISSN 1553-7390
    DOI 10.1371/journal.pgen.1000922
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  7. Article ; Online: NutriTRAILomics in prostate cancer: time to have two strings to one's bow.

    Farooqi, Ammad Ahmad / Rana, Aamir / Riaz, Asma M / Khan, Ammara / Ali, Muhammad / Javed, Sara / Mukhtar, Shahzeray / Minhaj, Sehrish / Rao, Javeria Rafique / Rajpoot, Javairia / Amber, Rafia / Javed, Fiza Asif / Waqar-Un-Nisa / Khanum, Reema / Bhatti, Shahzad

    Molecular biology reports

    2011  Volume 39, Issue 4, Page(s) 4909–4914

    Abstract: The astonishing development of broad genomics and proteomics tools have catalyzed a new era in both therapeutic interventions and nutrition in prostate cancer. The terms pharmacogenomics and nutrigenomics have been derived out of their genetic forbears ... ...

    Abstract The astonishing development of broad genomics and proteomics tools have catalyzed a new era in both therapeutic interventions and nutrition in prostate cancer. The terms pharmacogenomics and nutrigenomics have been derived out of their genetic forbears as large-scale genomics technologies have been established in the last decade. It is unquestionable that rationale of both disciplines is to individualize or personalize medicine and food and nutrition, and eventually health, by tailoring the drug or the food to the individual genotype. The purpose of this review is to significantly inspect results from current research concerning the mechanisms of action of phytonutrients and potential effects on prostate cancer. Substantial emerging data supports the synergistic adiministration of nutraceuticals with TRAIL in prostate cancer progression to circumvent TRAIL refractoriness. Nonetheless, developing novel scientific methods for discovery, validation, characterization and standardization of these multicomponent phyto-therapeutics is vital to their recognition into mainstream medicine. The key to interpret a personalized response is a greater comprehension of nutrigenomics, proteomics and metabolomics.
    MeSH term(s) Dietary Supplements ; Humans ; Male ; Models, Biological ; Nutrigenomics ; Prostatic Neoplasms/drug therapy ; TNF-Related Apoptosis-Inducing Ligand/pharmacology ; TNF-Related Apoptosis-Inducing Ligand/therapeutic use
    Chemical Substances TNF-Related Apoptosis-Inducing Ligand
    Language English
    Publishing date 2011-12-06
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 186544-4
    ISSN 1573-4978 ; 0301-4851
    ISSN (online) 1573-4978
    ISSN 0301-4851
    DOI 10.1007/s11033-011-1286-0
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