Article: RhoA GTPase regulates M-cadherin activity and myoblast fusion.
2006 Volume 17, Issue 2, Page(s) 749–759
Abstract: ... RhoAV14 perturbs the expression and localization of M-cadherin, a member of the Ca2+-dependent cell ... induces the entry of M-cadherin into a degradative pathway and thus decreases its stability in correlation ... with the monoubiquitination of M-cadherin. Moreover, p120 catenin association with M-cadherin is decreased in RhoAV14 ...
| Abstract | The Rho family of GTP-binding proteins plays critical roles during myogenesis induction. To elucidate their role later during myogenesis, we have analyzed RhoA function during myoblast fusion into myotubes. We find that RhoA activity is rapidly and transiently increased when cells are shifted into differentiation medium and then is decreased until myoblast fusion. RhoA activity must be down-regulated to allow fusion, because expression of a constitutively active form of RhoA (RhoAV14) inhibits this process. RhoAV14 perturbs the expression and localization of M-cadherin, a member of the Ca2+-dependent cell-cell adhesion molecule family that has an essential role in skeletal muscle cell differentiation. This mutant does not affect N-cadherin and other proteins involved in myoblast fusion, beta1-integrin and ADAM12. Active RhoA induces the entry of M-cadherin into a degradative pathway and thus decreases its stability in correlation with the monoubiquitination of M-cadherin. Moreover, p120 catenin association with M-cadherin is decreased in RhoAV14-expressing cells, which is partially reverted by the inhibition of the RhoA effector Rho-associated kinase ROCK. ROCK inhibition also restores M-cadherin accumulation at the cell-cell contact sites. We propose that the sustained activation of the RhoA pathway inhibits myoblast fusion through the regulation of p120 activity, which controls cadherin internalization and degradation. |
|---|---|
| MeSH term(s) | ADAM Proteins/metabolism ; ADAM12 Protein ; Animals ; Cadherins/analysis ; Cadherins/metabolism ; Catenins ; Cell Adhesion Molecules/metabolism ; Cell Fusion ; Cell Line ; Integrin beta1/metabolism ; Lysosomes/metabolism ; Mice ; Models, Biological ; Myoblasts/cytology ; Myoblasts/enzymology ; Myoblasts/metabolism ; Phosphoproteins/metabolism ; RNA Interference ; rhoA GTP-Binding Protein/metabolism ; rhoA GTP-Binding Protein/physiology |
| Chemical Substances | Cadherins ; Catenins ; Cell Adhesion Molecules ; Integrin beta1 ; Phosphoproteins ; delta catenin ; M-cadherin (142845-03-2) ; ADAM Proteins (EC 3.4.24.-) ; ADAM12 Protein (EC 3.4.24.-) ; Adam12 protein, mouse (EC 3.4.24.-) ; rhoA GTP-Binding Protein (EC 3.6.5.2) |
| Language | English |
| Publishing date | 2006-02 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 1098979-1 |
| ISSN | 1939-4586 ; 1059-1524 |
| ISSN (online) | 1939-4586 |
| ISSN | 1059-1524 |
| DOI | 10.1091/mbc.E05-04-0284 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
Full text online
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 2853: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG) |
|||
| Zs.MO 410: Show issues |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.