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Article ; Online: National priorities for oral health research, Peru 2022-2026: process, experiences and perspectives.

Echevarria-Goche, Adriana / Solis-Sánchez, Gilmer / Tuesta-Orbe, Lesly V / Andamayo-Flores, Christian / Vidal-Anzardo, Margot

Revista peruana de medicina experimental y salud publica

2023 Volume 40, Issue 3, Page(s) 354–363

Abstract: The National Institute of Health has, for the first time, identified National Priorities for Oral Health Research, this process was carried out by the Subdirectorate of Research and Laboratories of Noncommunicable Diseases of the National Center for ... ...

Title translation	Prioridades nacionales de investigación en salud bucal, Perú 2022-2026: proceso, experiencias y perspectivas.
Abstract	The National Institute of Health has, for the first time, identified National Priorities for Oral Health Research, this process was carried out by the Subdirectorate of Research and Laboratories of Noncommunicable Diseases of the National Center for Public Health with the technical advice of the Directorate of Research and Innovation in Health and in coordination with the Executive Directorate of Oral Health of the General Directorate of Strategic Interventions in Public Health of the Ministry of Health, using a participatory methodology with three key actors: researchers/specialists, experts and decision-makers. This article aims to describe the process used to identify these priorities, which consisted of five phases: i) identification of the strategic objectives of the Ministry of Health, ii) identification of needs in oral health research, iii) review by experts and assessment of needs according to criteria and v) presentation of the priorities. As a result, 12 priorities were obtained, which were subsequently approved by Ministerial Resolution No. 262-2022/MINSA, for a period of 2022-2026. In addition, we provide recommendations for future processes.
MeSH term(s)	Peru ; Oral Health ; Public Health
Language	English
Publishing date	2023-09-28
Publishing country	Peru
Document type	Journal Article
ZDB-ID	2120092-0
ISSN	1726-4642 ; 1726-4642
ISSN (online)	1726-4642
ISSN	1726-4642
DOI	10.17843/rpmesp.2023.403.12082
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Parasitosis intestinal

Margot Vidal-Anzardo / Martín Yagui Moscoso / María Beltrán Fabian

Anales de la Facultad de Medicina, Vol 81, Iss

Helmintos

2020 Volume 1

Abstract: expresado la necesidad de mapear la prevalencia de las parasitosis en América Latina y el Caribe. En el Perú no existen estudios que hayan medido la prevalencia de dicha patología. Objetivos. Determinar la frecuencia de parasitosis general y por tipo de ... ...

Abstract	expresado la necesidad de mapear la prevalencia de las parasitosis en América Latina y el Caribe. En el Perú no existen estudios que hayan medido la prevalencia de dicha patología. Objetivos. Determinar la frecuencia de parasitosis general y por tipo de helmintos en el año 2017 y la tendencia de los últimos 8 años previos, años a nivel nacional y por departamento. Métodos. Análisis de base de datos secundaria (Sistema de Información de Salud: HIS) del 2010 a 2017, que contiene el registro de las atenciones en los establecimientos de salud del Ministerio de Salud. Para el análisis de tendencias se utilizó modelos de regresión segmentada. Resultados. A nivel nacional la parasitosis general y por helmintos fue de 4,9% y de 3,3% respectivamente en el 2017; ambas presentaron una tendencia descendente, reduciéndose cada año 8,8% y 11,3% respectivamente. Igual ocurrió en el 68% (17/25) de las regiones, presentando una mayor reducción porcentual anual Amazonas, Huánuco, La Libertad, Cajamarca y Huancavelica. Áscaris y enterobiasis se presentaron con mayor frecuencia, representando en el 2017 cada una el 0,3%. Conclusiones. La prevalencia de parasitosis general, por grupo de helmintos y por tipo específico de helmintos, es menor a lo reportado en otros estudios, los cuales se realizaron en población escolar a diferencia de este estudio, el cual se realizó en población general donde el 57,2% fueron mayores de 18 años que acudieron a un establecimiento de salud.
Keywords	parasitosis intestinales ; helmintiasis ; prevalencia ; tendencias ; perú ; Medicine ; R ; Medicine (General) ; R5-920
Language	Spanish
Publishing date	2020-03-01T00:00:00Z
Publisher	Universidad Nacional Mayor de San Marcos
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: SARS-CoV-2 prevalence associated to low socioeconomic status and overcrowding in an LMIC megacity: A population-based seroepidemiological survey in Lima, Peru.

Reyes-Vega, Mary F / Soto-Cabezas, M Gabriela / Cárdenas, Fany / Martel, Kevin S / Valle, Andree / Valverde, Juan / Vidal-Anzardo, Margot / Falcón, María Elena / Munayco, César V

EClinicalMedicine

2021 Volume 34, Page(s) 100801

Abstract: Background: Worldwide, Peru has one of the highest infection fatality rates of COVID-19, and its capital city, Lima, accumulates roughly 50% of diagnosed cases. Despite surveillance efforts to assess the extent of the pandemic, reported cases and deaths ...

Abstract	Background: Worldwide, Peru has one of the highest infection fatality rates of COVID-19, and its capital city, Lima, accumulates roughly 50% of diagnosed cases. Despite surveillance efforts to assess the extent of the pandemic, reported cases and deaths only capture a fraction of its impact due to COVID-19's broad clinical spectrum. This study aimed to estimate the seroprevalence of SARS-CoV-2 in Lima, stratified by age, sex, region, socioeconomic status (SES), overcrowding, and symptoms. Methods: We conducted a multi-stage, population-based serosurvey in Lima, between June 28th and July 9th, 2020, after 115 days of the index case and after the first peak cases. We collected whole blood samples by finger-prick and applied a structured questionnaire. A point-of-care rapid serological test assessed IgM and IgG antibodies against SARS-CoV-2. Seroprevalence estimates were adjusted by sampling weights and test performance. Additionally, we performed RT-PCR molecular assays to seronegatives and estimated the infection prevalence. Findings: We enrolled 3212 participants from 797 households and 241 sample clusters from Lima in the analysis. The SARS-CoV-2 seroprevalence was 20·8% (95%CI 17·2-23·5), and the prevalence was 25·2% (95%CI 22·5-28·2). Seroprevalence was equally distributed by sex (aPR=0·96 [95%CI 0·85-1·09, Interpretation: This first estimate of SARS-CoV-2 seroprevalence in Lima shows an intense transmission scenario, despite the government's numerous interventions early established. Susceptibles across age groups show that physical distancing interventions must not be relaxed. SES and overcrowding households are associated with seroprevalence. This study highlights the importance of considering the existing social inequalities for implementing the response to control transmission in low- and middle-income countries.
Language	English
Publishing date	2021-03-30
Publishing country	England
Document type	Journal Article
ISSN	2589-5370
ISSN (online)	2589-5370
DOI	10.1016/j.eclinm.2021.100801
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Réplica: las pruebas de laboratorio para la identificación de SARS-CoV-2 en tiempos de pandemia en el Perú: algunas precisiones acerca del «rendimiento diagnóstico».

Vidal-Anzardo, Margot / Solis, Gilmer / Solari, Lely / Minaya, Gabriela / Ayala-Quintanilla, Beatriz / Astete-Cornejo, Jonh / Luque-Aguilar, Amparo / Jorge, Ana / Rojas, Nancy / Cardenas, Fanny / Soto, Alonso

Revista peruana de medicina experimental y salud publica

2020 Volume 37, Issue 3, Page(s) 577–579

Title translation	Reply: laboratory tests for identification of SARS-CoV-2 during pandemic times in Peru: some clarification regarding «diagnostic performance».
MeSH term(s)	COVID-19/diagnosis ; Clinical Laboratory Techniques ; Humans ; Pandemics ; Peru ; SARS-CoV-2
Language	Spanish
Publishing date	2020-05-28
Publishing country	Peru
Document type	Letter ; Comment
ZDB-ID	2120092-0
ISSN	1726-4642 ; 1726-4642
ISSN (online)	1726-4642
ISSN	1726-4642
DOI	10.17843/rpmesp.2020.373.5929
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Réplica: consideraciones sobre la evaluación en condiciones de campo de una prueba serológica rápida para detección de anticuerpos IgM e IgG contra SARS-CoV-2.

Revista peruana de medicina experimental y salud publica

2020 Volume 37, Issue 3, Page(s) 573–574

Title translation	Reply: considerations on the evaluation under field conditions of a rapid test for detection of IgM and IgG antibodies against SARS-CoV-2.
MeSH term(s)	Antibodies, Viral ; COVID-19 ; Humans ; Immunoglobulin G ; Immunoglobulin M ; Pandemics ; SARS-CoV-2
Chemical Substances	Antibodies, Viral ; Immunoglobulin G ; Immunoglobulin M
Language	Spanish
Publishing date	2020-10-09
Publishing country	Peru
Document type	Letter ; Comment
ZDB-ID	2120092-0
ISSN	1726-4642 ; 1726-4634
ISSN (online)	1726-4642
ISSN	1726-4634
DOI	10.17843/rpmesp.2020.373.5918
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Article ; Online: Evaluation of a rapid serological test for detection of IgM and igG antibodies against SARS-CoV-2 under field conditions.

Revista peruana de medicina experimental y salud publica

2020 Volume 37, Issue 2, Page(s) 203–209

Abstract: Objective: To determine the additional diagnostic performance of a rapid serological test for detection of IgM and IgG antibodies compared to the real-time polymerase chain reaction (RT-PCR) test; for detection of SARS-CoV-2.: Materials and methods: ... ...

Title translation	Evaluación en condiciones de campo de una prueba serológica rápida para detección de anticuerpos IgM e IgG contra SARS-CoV-2.
Abstract	Objective: To determine the additional diagnostic performance of a rapid serological test for detection of IgM and IgG antibodies compared to the real-time polymerase chain reaction (RT-PCR) test; for detection of SARS-CoV-2. Materials and methods: A cross-sectional study was carried out including patients hospitalized for COVID-19 in 3 hospitals, health workers exposed to the infection and outpatients who met suspicious case criteria, all of which underwent the molecular test (RT-PCR) and the rapid serological test. The additional diagnostic performance of rapid serological test was evaluated in comparison to molecular tests. Likewise, an approximation was made to the sensitivity and specificity of the rapid serological test. Results: 144 people were included. With the rapid test, 19.4% of positive results were obtained compared to 11.1% in the molecular test (p = 0.03). The rapid serological test detected 21 cases that had been negative by the initial (RT-PCR), providing an additional diagnostic performance of 56.8% compared to the RT-PCR. The additional diagnostic performance was 50.0% during the first week, 70.0% during the second week and 50.0% during the third week of symptom onset. The sensitivity of the rapid serological test was 43.8% and the specificity of 98.9%. Conclusions: The rapid serological test was able to detect a greater number of cases than those detected by the molecular test especially after the second week of onset of symptoms. It also showed high specificity. It is therefore useful as a complementary test to RT-PCR, especially during the second and third week of illness.
MeSH term(s)	Adult ; Betacoronavirus/isolation & purification ; COVID-19 ; COVID-19 Testing ; COVID-19 Vaccines ; Clinical Laboratory Techniques ; Coronavirus Infections/diagnosis ; Coronavirus Infections/immunology ; Cross-Sectional Studies ; Female ; Humans ; Immunoglobulin G/blood ; Immunoglobulin M/blood ; Male ; Middle Aged ; Pandemics ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/immunology ; Real-Time Polymerase Chain Reaction/methods ; SARS-CoV-2 ; Sensitivity and Specificity ; Serologic Tests/methods
Chemical Substances	COVID-19 Vaccines ; Covid-19 aAPC vaccine ; Immunoglobulin G ; Immunoglobulin M
Keywords	covid19
Language	English
Publishing date	2020-08-28
Publishing country	Peru
Document type	Comparative Study ; Journal Article ; Multicenter Study
ZDB-ID	2120092-0
ISSN	1726-4642 ; 1726-4642
ISSN (online)	1726-4642
ISSN	1726-4642
DOI	10.17843/rpmesp.2020.372.5534
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Article ; Online: Standardization and validation of an in house RT-LAMP molecular test for the diagnosis of SARS-CoV-2.

Escalante-Maldonado, Oscar / Vidal-Anzardo, Margot / Donaires, Fernando / Solis-Sanchez, Gilmer / Gallesi, Italo / Pampa-Espinoza, Luis / Huaringa, Maribel / Rojas-Serrano, Nancy / García, Coralith / Angles-Yanqui, Eddie / Gavilán, Ronnie Gustavo / Durães-Carvalho, Ricardo / Mendez-Rico, Jairo / Cabezas, César / Marques-Simas, Paulo Vitor

Revista peruana de medicina experimental y salud publica

2021 Volume 38, Issue 1, Page(s) 7–16

Abstract: Objectives: To standardize and validate an in-house RT-LAMP test for the detection of SARS-CoV-2, based on laboratory and field assays using samples from COVID-19 suspected patients.: Materials and methods: An in-house SARS-CoV-2 RT-LAMP molecular ... ...

Title translation	Estandarización y validación de una prueba molecular RT-LAMP in house para el diagnóstico de SARS-CoV-2.
Abstract	Objectives: To standardize and validate an in-house RT-LAMP test for the detection of SARS-CoV-2, based on laboratory and field assays using samples from COVID-19 suspected patients. Materials and methods: An in-house SARS-CoV-2 RT-LAMP molecular test was standardized, establishing the detection limit with Vero cells of isolated Peruvian strains of SARS-CoV-2, and the robustness to various concentrations of primers. The laboratory validation was performed with 384 nasal and pharyngeal swab samples (UFH) obtained between March and July 2020. The field validation was performed with 383 UFH obtained from COVID-19 suspected symptomatic cases. All samples were tested by RT-LAMP and RT-qPCR. The RT-qPCR was considered as the reference standard test. The concordance measures and diagnostic performance were calculated. Results: The detection limit was consistent in cases with Ct <30 in both tests, showing efficiency to detect up to 1000 copies/μL of the target gene. Robustness was evidenced with half of the primer concentrations and 20 μL of final volume. Absence of amplification was identified for other HCoVs. Concordance showed a kappa index of 0.88 (95% CI: 0.83-0.93) and 0.89 (95% CI: 0.84 - 0.94) in laboratory and field settings, respectively. The sensitivity value in the laboratory was 87.4% (95% CI: 80.8 - 92.4) and 88.1% in the field (95% CI: 81.6 - 92.9). The specificity value in both settings was 98.8% (95% CI: 96.4-99.7). Conclusions: The in-house SARS-CoV-2 RT-LAMP test was successfully validated based on its adequate robustness, no cross-reactions, good concordance, and diagnostic performance compared to RT-qPCR.
MeSH term(s)	Animals ; COVID-19 ; Chlorocebus aethiops ; Humans ; Molecular Diagnostic Techniques ; Nucleic Acid Amplification Techniques ; RNA, Viral ; Reference Standards ; SARS-CoV-2 ; Sensitivity and Specificity ; Vero Cells
Chemical Substances	RNA, Viral
Language	English
Publishing date	2021-06-25
Publishing country	Peru
Document type	Journal Article
ZDB-ID	2120092-0
ISSN	1726-4642 ; 1726-4642
ISSN (online)	1726-4642
ISSN	1726-4642
DOI	10.17843/rpmesp.2021.381.7154
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: SARS-CoV-2 prevalence associated to low socioeconomic status and overcrowding in an LMIC megacity

Mary F. Reyes-Vega / M.Gabriela Soto-Cabezas / Fany Cárdenas / Kevin S. Martel / Andree Valle / Juan Valverde / Margot Vidal-Anzardo / María Elena Falcón / César V. Munayco

EClinicalMedicine, Vol 34, Iss , Pp 100801- (2021)

A population-based seroepidemiological survey in Lima, Peru

2021

Abstract	Background: Worldwide, Peru has one of the highest infection fatality rates of COVID-19, and its capital city, Lima, accumulates roughly 50% of diagnosed cases. Despite surveillance efforts to assess the extent of the pandemic, reported cases and deaths only capture a fraction of its impact due to COVID-19′s broad clinical spectrum. This study aimed to estimate the seroprevalence of SARS-CoV-2 in Lima, stratified by age, sex, region, socioeconomic status (SES), overcrowding, and symptoms. Methods: We conducted a multi-stage, population-based serosurvey in Lima, between June 28th and July 9th, 2020, after 115 days of the index case and after the first peak cases. We collected whole blood samples by finger-prick and applied a structured questionnaire. A point-of-care rapid serological test assessed IgM and IgG antibodies against SARS-CoV-2. Seroprevalence estimates were adjusted by sampling weights and test performance. Additionally, we performed RT-PCR molecular assays to seronegatives and estimated the infection prevalence. Findings: We enrolled 3212 participants from 797 households and 241 sample clusters from Lima in the analysis. The SARS-CoV-2 seroprevalence was 20·8% (95%CI 17·2–23·5), and the prevalence was 25·2% (95%CI 22·5–28·2). Seroprevalence was equally distributed by sex (aPR=0·96 [95%CI 0·85–1·09, p = 0·547]) and across all age groups, including ≥60 versus ≤11 years old (aPR=0·96 [95%CI 0·73–1·27, p = 0·783]). A gradual decrease in SES was associated with higher seroprevalence (aPR=3·41 [95%CI 1·90–6·12, p<0·001] in low SES). Also, a gradual increase in the overcrowding index was associated with higher seroprevalence (aPR=1·99 [95%CI 1·41–2·81, p<0·001] in the fourth quartile). Seroprevalence was also associated with contact with a suspected or confirmed COVID-19 case, whether a household member (48·9%, aPR=2·67 [95%CI 2·06–3·47, p<0·001]), other family members (27·3%, aPR=1·66 [95%CI 1·15–2·40, p = 0·008]) or a workmate (34·1%, aPR=2·26 [95%CI 1·53–3·35, p<0·001]). More than half of ...
Keywords	SARS-CoV-2 ; COVID-19 ; Prevalence ; Seroprevalence ; Population-based ; Peru ; Medicine (General) ; R5-920
Subject code	360
Language	English
Publishing date	2021-04-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Reply

Margot Vidal-Anzardo / Gilmer Solis / Lely Solari / Gabriela Minaya / Beatriz Ayala-Quintanilla / John Astete-Cornejo / Amparo Luque-Aguilar / Ana Jorge / Nancy Rojas / Fanny Cardenas / Alonso Soto

Revista Peruana de Medicina Experimental y Salud Pública, Vol 37, Iss 3, Pp 577-

laboratory tests for identification of SARS-CoV-2 during pandemic times in Peru: some clarification regarding «diagnostic performance»

2020 Volume 9

Abstract: ...

Abstract	-
Keywords	Medicine ; R ; Medicine (General) ; R5-920
Language	Spanish
Publishing date	2020-09-01T00:00:00Z
Publisher	Instituto Nacional de Salud
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Reply

Margot Vidal-Anzardo / Gilmer Solis / Lely Solari / Gabriela Minaya / Beatriz Ayala-Quintanilla / Jonh Astete-Cornejo / Amparo Luque-Aguilar / Ana Jorge / Nancy Rojas / Fanny Cardenas / Alonso Soto

Revista Peruana de Medicina Experimental y Salud Pública, Vol 37, Iss 3, Pp 573-

considerations on the evaluation under field conditions of a rapid test for detection of IgM and IgG antibodies against SARS-CoV-2

2020 Volume 4

Abstract: ...

Abstract	-
Keywords	Medicine ; R ; Medicine (General) ; R5-920
Language	Spanish
Publishing date	2020-09-01T00:00:00Z
Publisher	Instituto Nacional de Salud
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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