Article ; Online: C-MYC induces idiopathic pulmonary fibrosis via modulation of miR-9-5p-mediated TBPL1.
2022 Volume 93, Page(s) 110274
Abstract: We sought to pinpoint the potential role of C-MYC in pulmonary fibroblast proliferation in idiopathic pulmonary fibrosis (IPF) and its mechanism. A mouse model of IPF was established by injection of bleomycin. C-MYC and miR-9-5p expression was determined ...
| Abstract | We sought to pinpoint the potential role of C-MYC in pulmonary fibroblast proliferation in idiopathic pulmonary fibrosis (IPF) and its mechanism. A mouse model of IPF was established by injection of bleomycin. C-MYC and miR-9-5p expression was determined by RT-qPCR and Western blot analysis. The interaction among C-MYC, miR-9-5p, and TBPL1 was detected by ChIP assay and dual luciferase reporter gene assay. After alteration of C-MYC, miR-9-5p, and TBPL1, their roles in pulmonary fibrosis and collagen fiber deposition in mice as well as proliferation and differentiation of pulmonary fibroblasts were assessed. Upregulated C-MYC expression was seen in the lung tissues of IPF mice and its silencing retarded IPF in mice. C-MYC could activate miR-9-5p that negatively regulated TBPL1 expression. Up-regulated C-MYC promoted proliferation and differentiation of pulmonary fibroblasts by inhibiting TBPL1 via activation of miR-9-5p, thus triggering IPF. Moreover, in the lung tissues-derived cells of IPF mice, C-MYC inhibitor, 10,058-F4, was observed to inhibit miR-9-5p expression, thereby repressing pulmonary fibrosis by up-regulating TBPL1. Our data provided evidence pinpointed the aggravative role of C-MYC in IPF by activating miR-9-5p to regulate TBPL1 expression. |
|---|---|
| MeSH term(s) | Animals ; Bleomycin/metabolism ; Bleomycin/pharmacology ; Cell Proliferation ; Fibroblasts/metabolism ; Idiopathic Pulmonary Fibrosis/genetics ; Idiopathic Pulmonary Fibrosis/metabolism ; Lung/metabolism ; Mice ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Proto-Oncogene Proteins c-myc/metabolism ; TATA Box Binding Protein-Like Proteins/metabolism |
| Chemical Substances | MIRN9 microRNA, mouse ; MicroRNAs ; Myc protein, mouse ; Proto-Oncogene Proteins c-myc ; TATA Box Binding Protein-Like Proteins ; Tbpl1 protein, mouse ; Bleomycin (11056-06-7) |
| Language | English |
| Publishing date | 2022-02-03 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 1002702-6 |
| ISSN | 1873-3913 ; 0898-6568 |
| ISSN (online) | 1873-3913 |
| ISSN | 0898-6568 |
| DOI | 10.1016/j.cellsig.2022.110274 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 2579: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.