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Article ; Online: Retrospective evaluation of viral respiratory tract infections in a university hospital in Ankara, Turkey (2016-2019).

Altay-Kocak, Aylin / Sarzhanova, Shakhnoza / Tapisiz, Anil / Dizbay, Murat / Basustaoglu, Ahmet / Bozdayi, Gulendam

Journal of infection in developing countries

2022 Volume 16, Issue 5, Page(s) 857–863

Abstract: Introduction: Viruses are responsible for two-thirds of all acute respiratory tract infections. This study aims to retrospectively detect respiratory tract viruses in patients from all age groups who visited the hospital.: Methodology: A total of ... ...

Abstract	Introduction: Viruses are responsible for two-thirds of all acute respiratory tract infections. This study aims to retrospectively detect respiratory tract viruses in patients from all age groups who visited the hospital. Methodology: A total of 1592 samples from 1416 patients with respiratory tract symptoms were sent from several clinics to the Molecular Microbiology Laboratory at Gazi University Hospital from February 2016 to January 2019. Nucleic acid extraction from nasopharyngeal swabs, throat swabs or bronchoalveolar lavage (BAL) samples sent to our laboratory was done using a commercial automated system. Extracted nucleic acids were amplified by a commercial multiplex-real time Polymerase Chain Reaction (PCR) method, which can detect 18 viral respiratory pathogens. Results: Among 1592 samples, 914 (57.4%) were positive for respiratory viruses. The most prevalent were rhinovirus (25.2%) and influenza A virus (12.1%), the least prevalent was the bocavirus (2.6%). Rhinovirus was the most detected as a single agent (21.2%, 194/914) among all positive cases, followed by coronavirus (9.3%, 85/914). The detection rates of coronavirus, human adenovirus, respiratory syncytial virus A/B, human parainfluenza viruses, human metapneumovirus-A/B, human parechovirus, enterovirus and influenza B virus were 9.9%, 8%, 7.7%, 5%, 3.4%, 3.1%, 3%, and 2.8%, respectively. Conclusions: The most detected viral agents in our study were influenza A virus and rhinovirus. Laboratory diagnosis of respiratory viruses is helpful to prevent unnecessary antibiotic use and is essential in routine diagnostics for antiviral treatment. Multiplex Real-time PCR method is fast and useful for the diagnosis of viral respiratory infections.
MeSH term(s)	Coronavirus ; Coronavirus Infections/epidemiology ; Enterovirus Infections/epidemiology ; Hospitals, University ; Humans ; Influenza A virus ; Influenza, Human/epidemiology ; Picornaviridae Infections/epidemiology ; Respiratory Syncytial Viruses ; Respiratory Tract Infections/diagnosis ; Respiratory Tract Infections/epidemiology ; Respiratory Tract Infections/virology ; Retrospective Studies ; Turkey/epidemiology
Language	English
Publishing date	2022-05-30
Publishing country	Italy
Document type	Journal Article
ZDB-ID	2394024-4
ISSN	1972-2680 ; 2036-6590
ISSN (online)	1972-2680
ISSN	2036-6590
DOI	10.3855/jidc.14427
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Article: Serum Nickel and Titanium Levels after Transcatheter Closure of Atrial Septal Defects with Amplatzer Septal Occluder.

Elkiran, Ozlem / Karakurt, Cemsit / Kocak, Gulendam / Taskapan, Cagatay

Cardiology research and practice

2019 Volume 2019, Page(s) 7891746

Abstract: Introduction: There is a concern about release of nickel and titanium after implantation of nitinol-containing devices.: Objective: To evaluate serum nickel and titanium release after implantation of Amplatzer occluder.: Materials and methods: In ... ...

Abstract	Introduction: There is a concern about release of nickel and titanium after implantation of nitinol-containing devices. Objective: To evaluate serum nickel and titanium release after implantation of Amplatzer occluder. Materials and methods: In 38 pediatric patients with no history of nickel sensitivity, blood samples were drawn 24 hours before and 24 hours, 1, 3, 6, and 12 months after implantation. Nickel and titanium concentrations were measured by atomic absorption spectrophotometry. Results: The median serum nickel level which was 0.44 ng/mL before the implantation increased to 1.01 ng/mL 24 hours after implantation and 1.72 ng/mL one month after implantation. The maximum level was detected 3 months after implantation, with a median level of 1.96 ng/mL. During follow-up, the nickel levels decreased to those measured before implantation. Serum nickel levels at the 24th hour, 1st month, and 3rd month following implantation were found to have increased significantly. No patients showed a detectable serum titanium level. Discussion: This is the first study that evaluated both serum nickel and titanium release after implantation of the Amplatzer occluder. Our study shows that nickel is released from the device in the first few months after implantation. Therefore, in patients with nickel allergy, other devices may be considered.
Language	English
Publishing date	2019-01-02
Publishing country	United States
Document type	Journal Article
ZDB-ID	2506187-2
ISSN	2090-0597 ; 2090-8016
ISSN (online)	2090-0597
ISSN	2090-8016
DOI	10.1155/2019/7891746
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Article ; Online: Incidence and clinical characteristics of acute rheumatic fever in Turkey: Results of a nationwide multicentre study.

Gürses, Dolunay / Koçak, Gülendam / Tutar, Ercan / Özbarlas, Nazan

Journal of paediatrics and child health

2021 Volume 57, Issue 12, Page(s) 1949–1954

Abstract: Aim: To evaluate the incidence and clinical features of acute rheumatic fever (ARF) in Turkey, following the revised Jones criteria in 2015.: Methods: This multicentre study was designed by the Acquired Heart Diseases Working Group of the Turkish ... ...

Abstract	Aim: To evaluate the incidence and clinical features of acute rheumatic fever (ARF) in Turkey, following the revised Jones criteria in 2015. Methods: This multicentre study was designed by the Acquired Heart Diseases Working Group of the Turkish Pediatric Cardiology and Pediatric Cardiac Surgery Association in 2016. The data during the first attack of 1103 ARF patients were collected from the paediatric cardiologists between 1 January 2016 and 31 December 2016. Results: Turkey National Institute of Statistics records of 2016 were used for the determination of ARF incidence with regard to various cities and regions separately. The estimated incidence rate of ARF was 8.84/100 000 in Turkey. The ARF incidence varied considerably among different regions. The highest incidence was found in the Eastern Anatolia Region as 14.4/100 000, and the lowest incidence was found in the Black Sea Region as 3.3/100 000 (P < 0.05). Clinical carditis was the most common finding. The incidence of clinical carditis, subclinical carditis, polyarthritis, aseptic monoarthritis, polyarthralgia and Sydenham's Chorea was 53.5%, 29.1%, 52.8%, 10.3%, 18.6% and 7.9%, respectively. The incidences of clinical carditis, subclinical carditis, polyarthritis and polyarthralgia were found to be significantly different among different regions (P < 0.05). Conclusion: The findings of this nationwide screening of ARF suggest that Turkey should be included in the moderate-risk group.
MeSH term(s)	Acute Disease ; Child ; Humans ; Incidence ; Myocarditis ; Retrospective Studies ; Rheumatic Fever/diagnosis ; Rheumatic Fever/epidemiology ; Rheumatic Heart Disease ; Turkey/epidemiology
Language	English
Publishing date	2021-07-06
Publishing country	Australia
Document type	Journal Article ; Multicenter Study
ZDB-ID	1024476-1
ISSN	1440-1754 ; 1034-4810
ISSN (online)	1440-1754
ISSN	1034-4810
DOI	10.1111/jpc.15619
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Article ; Online: The prevalence and distribution of human papillomavirus in 4267 Turkish women with or without cervical lesions: A hospital-based study.

Altay-Kocak, Aylin / Kazanci, Ferah / Dogu-Tok, Canan / Onan, Anil / Erdem, Ozlem / Ozkan, Secil / Bozdayi, Gulendam

Journal of medical virology

2022 Volume 94, Issue 10, Page(s) 5026–5032

Abstract: In the present study, it was aimed to screen the genotypes of human papillomavirus (HPV) retrospectively in women with gynecological symptoms who were admitted to a tertiary care university hospital in Ankara, Turkey. A total of 4267 cervical swab ... ...

Abstract	In the present study, it was aimed to screen the genotypes of human papillomavirus (HPV) retrospectively in women with gynecological symptoms who were admitted to a tertiary care university hospital in Ankara, Turkey. A total of 4267 cervical swab samples of women aged 18-79 years were sent to Medical Virology Laboratory from January 2017 to November 2020. Nucleic acid extraction and amplification of samples were done by an automated system. The test can detect 14 high-risk HPV (HR-HPV) types in a single analysis that performs a real-time polymerase chain reaction, by providing individual results on the highest-risk genotypes HPV 16 and HPV 18 and pooled results on other high-risk genotypes (OHR-HPV) (31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68). HPV DNA positivity was detected in 14.2% (605/4267) of the samples. HPV type 16 and type 18 were detected in 2.4% and 0.7% of the samples, respectively. OHR-HPV types were found in 8.8% of the samples. Of the 1.9% and 0.4% samples had mixed types with type 16+ OHR-HPV and type 18+ OHR-HPV, respectively. The results of this study presented the rates of HR-HPV genotypes of a university hospital in Ankara, over a 4-year period. It was observed that the positivity rate of type 18 is decreasing and some OHR-HPV types are increasing. HPV vaccination is not in the national immunization program in Turkey yet, however, HPV vaccines are available and the vaccination rates for women are increasing.
MeSH term(s)	Alphapapillomavirus/genetics ; DNA, Viral/analysis ; DNA, Viral/genetics ; Female ; Genotype ; Hospitals ; Human papillomavirus 16/genetics ; Human papillomavirus 18/genetics ; Humans ; Papillomaviridae/genetics ; Papillomavirus Infections ; Papillomavirus Vaccines ; Prevalence ; Retrospective Studies ; Turkey/epidemiology ; Uterine Cervical Neoplasms
Chemical Substances	DNA, Viral ; Papillomavirus Vaccines
Language	English
Publishing date	2022-06-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	752392-0
ISSN	1096-9071 ; 0146-6615
ISSN (online)	1096-9071
ISSN	0146-6615
DOI	10.1002/jmv.27921
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Article: Possible association between fluconazole administration and acute hyperkalemia in a critically ill cyanotic infant.

Elkiran, Ozlem / Karakurt, Cemsit / Kocak, Gulendam / Tabel, Yilmaz / Gungor, Serdal

Archives of medical science : AMS

2015 Volume 11, Issue 1, Page(s) 235–236

Language	English
Publishing date	2015-03-14
Publishing country	Poland
Document type	Journal Article
ZDB-ID	2203781-0
ISSN	1734-1922
ISSN	1734-1922
DOI	10.5114/aoms.2015.49215
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Article ; Online: Emergence of rotavirus G9 in 2012, as the dominant genotype in Turkish children with diarrhea, in a university hospital in Ankara

Koçak Aylin Altay / Aydın Merve / Matsumoto Takashi / Yahiro Takaaki / Dalgıç Buket / Bozdayi Gulendam / Ahmed Kamruddin

Romanian Journal of Laboratory Medicine, Vol 27, Iss 2, Pp 209-

2019 Volume 218

Abstract: Introduction: Rotavirus infection is a major cause of morbidity and mortality in infants and young children with diarrhea throughout the world. ...

Abstract	Introduction: Rotavirus infection is a major cause of morbidity and mortality in infants and young children with diarrhea throughout the world.
Keywords	children ; genotype ; rotavirus infection ; turkey ; Medicine ; R
Language	English
Publishing date	2019-04-01T00:00:00Z
Publisher	Sciendo
Document type	Article ; Online
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Article: The Role of Human Parvovirus B19 in the Pediatric Patients with Pancytopenia?

Colak, Meryem / Kocak, Aylin A / Dinc, Bedia / Kaya, Zuhre / Kocak, Ulker / Yenicesu, İdil / Bozdayi, Gulendam

Clinical laboratory

2020 Volume 65, Issue 12

Abstract: Background: Parvoviruses are small DNA viruses causing erythema infectiosum, which is known as the fifth disease. The aim of this study was to investigate the presence of Parvovirus B19 DNA by Real-Time-PCR retrospectively in clinical samples of ... ...

Abstract	Background: Parvoviruses are small DNA viruses causing erythema infectiosum, which is known as the fifth disease. The aim of this study was to investigate the presence of Parvovirus B19 DNA by Real-Time-PCR retrospectively in clinical samples of children diagnosed as acute leukemia and aplastic anemia when investigating the cause of pancytopenia and to investigate its relationship with the clinical manifestations. Methods: The study samples were collected between March 2014 and March 2018 in Gazi University, Faculty of Medicine, Department of Pediatric Hematology. Sixty pediatric patients; 37 males and 23 females, were included in the study. Nucleic acid isolation was performed by using MagNA-Pure Compact Nucleic Acid Isolation Kit (Roche, Germany). Extracted DNA was studied with LightCycler® 2.0 using the Real-Time PCR method and LightCycler® Parvovirus B19 Quantification Kit (Roche, Germany), and the results were evaluated quantitatively. Parvovirus B19 DNA detection interval of the kit was 101 - 106 copies/mL. Results: Sixty serum samples were investigated and 8.3% (5/60) Parvovirus B19 DNA positivity was determined. Of the five patients with Parvovirus B19 DNA positivity, three had acute lymphoblastic leukemia and two were diagnosed as aplastic anemia. Regarding viral load; 2/5, 1/5, 1/5, and 1/5 of the samples had a viral load of 102, 103, 104, and 105 copies/mL, respectively. Parvovirus B19 DNA positivity was detected in samples from March (2/5), April (2/5), and August (1/5). Conclusions: Patients with acute leukemia and aplastic anemia in childhood using immunosuppressive drugs, blood, and blood products during chemotherapy, encounter Parvovirus B19 infections in the follow-up period and are diagnosed by serological and molecular methods. As a result of the study, we suggest that the detection of Parvovirus B19 DNA by Real-Time PCR method in children being admitted with pancytopenia and diagnosed as acute leukemia and aplastic anemia is useful in the follow-up and treatment.
MeSH term(s)	Adolescent ; Anemia, Aplastic/complications ; Anemia, Aplastic/diagnosis ; Anemia, Aplastic/drug therapy ; Child ; Child, Preschool ; DNA, Viral/genetics ; DNA, Viral/isolation & purification ; Erythema Infectiosum/complications ; Erythema Infectiosum/diagnosis ; Erythema Infectiosum/virology ; Female ; Humans ; Immunosuppressive Agents/therapeutic use ; Infant ; Male ; Pancytopenia/blood ; Pancytopenia/complications ; Pancytopenia/diagnosis ; Parvovirus B19, Human/genetics ; Parvovirus B19, Human/physiology ; Precursor Cell Lymphoblastic Leukemia-Lymphoma/complications ; Precursor Cell Lymphoblastic Leukemia-Lymphoma/diagnosis ; Precursor Cell Lymphoblastic Leukemia-Lymphoma/drug therapy ; Real-Time Polymerase Chain Reaction ; Retrospective Studies
Chemical Substances	DNA, Viral ; Immunosuppressive Agents
Language	English
Publishing date	2020-01-08
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1307629-2
ISSN	1433-6510 ; 0941-2131
ISSN	1433-6510 ; 0941-2131
DOI	10.7754/Clin.Lab.2019.190311
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Article: Detection of HPV DNA in Esophageal Lesions: a Cross-Sectional Study.

Dinc, Bedia / Altay-Kocak, Aylin / Aydog, Gulden / Kuran, Sedef / Akoglu, Musa / Ozkan, Secil / Bozdayi, Gulendam

Clinical laboratory

2020 Volume 66, Issue 3

Abstract: Background: Several studies have documented human papillomavirus (HPV) in extra-cervical tumors. We aimed to detect HPV type 16 and HPV other than type 16 (OT-16) DNA in esophageal papilloma and esophagus squamous cell carcinoma (ESCC) samples and to ... ...

Abstract	Background: Several studies have documented human papillomavirus (HPV) in extra-cervical tumors. We aimed to detect HPV type 16 and HPV other than type 16 (OT-16) DNA in esophageal papilloma and esophagus squamous cell carcinoma (ESCC) samples and to compare clinicopathological features of HPV positive and negative patients. Methods: Materials were obtained from a tertiary care public hospital and studied in an university hospital for this cross-sectional study. Seventy-six tissue samples (50 papilloma and 26 ESCC) were included. After deparaffinization by xylene and DNA extraction by phenol chloroform-isoamyl-alcohol, 76 samples were studied with a G6PDH control kit. Forty-four papilloma and 21 ESCC samples with enough tissues were studied for HPV DNA. HPV OT-16 DNA and HPV type 16 were detected by real time-polymerase chain reaction. Results: Twelve (27.3%) and one (2.3%) of the papilloma samples were HPV type 16 and other than type 16 positive, respectively. Eleven (52.4%) and one (4.8%) of ESCC samples were HPV type 16 and mixed type positive, respectively. Conclusions: We suggest that HPV infection is common in esophageal papilloma and ESCC. Due to the wellknown association of HPV with premalignant and malignant conditions, follow-up of these patients accompanied by HPV should be implemented.
MeSH term(s)	Adult ; Aged ; Cross-Sectional Studies ; DNA, Viral/analysis ; DNA, Viral/isolation & purification ; Esophageal Neoplasms/epidemiology ; Esophageal Neoplasms/virology ; Esophageal Squamous Cell Carcinoma/epidemiology ; Esophageal Squamous Cell Carcinoma/virology ; Female ; Human papillomavirus 16/genetics ; Humans ; Male ; Middle Aged ; Papillomaviridae/genetics ; Papillomavirus Infections/complications ; Papillomavirus Infections/epidemiology ; Papillomavirus Infections/virology ; Retrospective Studies ; Young Adult
Chemical Substances	DNA, Viral
Language	English
Publishing date	2020-03-11
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1307629-2
ISSN	1433-6510 ; 0941-2131
ISSN	1433-6510 ; 0941-2131
DOI	10.7754/Clin.Lab.2019.190506
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Article ; Online: Immunogenicity and safety of the CoronaVac and BNT162b2 Covid-19 vaccine in patients with inflammatory rheumatic diseases and healthy adults: comparison of different vaccines.

Batıbay, Sevilay / Ulucaköy, Rezan Koçak / Günendi, Zafer / Fidan, Işıl / Bozdayı, Gülendam / Göğüş, Feride Nur

Inflammopharmacology

2022 Volume 30, Issue 6, Page(s) 2089–2096

Abstract: Objectives: To determine the seroconversion (SC) rate after CoronaVac and BNT162b2 vaccines in adults with inflammatory rheumatic disease (IRD).: Methods: Patients who were followed up with IRD and who received two doses of either CoronaVac or ... ...

Abstract	Objectives: To determine the seroconversion (SC) rate after CoronaVac and BNT162b2 vaccines in adults with inflammatory rheumatic disease (IRD). Methods: Patients who were followed up with IRD and who received two doses of either CoronaVac or BNT162b2 vaccines were included in this prospective observational single-center study. Subjects with two doses of CoronaVac or BNT162b2 without known IRD were included in the healthy controls. The blood samples were taken at a minimum of two and a maximum of 12 weeks after the second dose of vaccine. Results: A total of 81 patients with IRD (61 CoronaVac, 20 BNT162b2) and 100 healthy controls (70 CoronaVac, 30 BNT162b2) were included. The SC rate was slightly lower among patients with IRD versus controls (84 vs 97%, p = 0.002). The SC rate was 100% in all participants who received BNT162b2 both in the patient and control group. The IgG antibody level after CoronaVac in the patient group was significantly lower than both the BNT162b2 (p = 0.031) and the healthy group (p < 0.001). Among patients with IRD, those on rituximab (RTX) (12/81,14.8%) had significantly less SC rate (5/12, 41.7%). The median neutralizing antibody titers were significantly higher in patients with BNT162b2 compared with CoronaVac (1.97 vs. 16.34, p < 0.001). Conclusions: This study showed that all patients with BNT162b2 vaccine developed immunogenicity in patients with IRD, while there was a decreased antibody response with CoronaVac vaccine compared to that of BNT162b2. In particular, RTX significantly reduces the SC rate.
MeSH term(s)	Adult ; Humans ; BNT162 Vaccine ; COVID-19/prevention & control ; COVID-19 Vaccines/adverse effects ; Rheumatic Diseases/drug therapy ; Vaccines
Chemical Substances	BNT162 Vaccine ; COVID-19 Vaccines ; Vaccines
Language	English
Publishing date	2022-10-25
Publishing country	Switzerland
Document type	Observational Study ; Journal Article
ZDB-ID	1080058-x
ISSN	1568-5608 ; 0925-4692
ISSN (online)	1568-5608
ISSN	0925-4692
DOI	10.1007/s10787-022-01089-6
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Article ; Online: Tissue Doppler, strain, and strain rate measurements assessed by two-dimensional speckle-tracking echocardiography in healthy newborns and infants.

Elkiran, Ozlem / Karakurt, Cemsit / Kocak, Gulendam / Karadag, Ahmet

Cardiology in the young

2014 Volume 24, Issue 2, Page(s) 201–211

Abstract: Objectives: To evaluate cardiac maturational and haemodynamic alteration in healthy newborns and infants and determine reference values in this period using tissue Doppler, strain, and strain rate echocardiography.: Material and methods: The study ... ...

Abstract	Objectives: To evaluate cardiac maturational and haemodynamic alteration in healthy newborns and infants and determine reference values in this period using tissue Doppler, strain, and strain rate echocardiography. Material and methods: The study included 149 healthy subjects. Babies from 1 day to 3 months were selected from the well-baby nursery department, and infants were selected from paediatric clinics during routine visits for health maintenance. Subjects were allocated to four groups: preterm (36-37 weeks, n = 32), term (≥ 38 weeks, n = 32), 1 month of age (n = 47), and 3 months of age (n = 38). Standard echocardiographic evaluations, pulsed wave Doppler, tissue Doppler echocardiography, strain, and strain rate studies were applied by the same person using a MyLab50 echo machine. Longitudinal and circumferential systolic strain and strain rate measurements were assessed by two-dimensional speckle-tracking echocardiography in all subjects. Results: The longitudinal systolic velocity, strain, and strain rate values derived from left ventricle apical four-, three-, and two-chamber images, and circumferential systolic velocity, strain, and strain rate values derived from left ventricle short-axis images decreased from the base to the apex in all subjects (p < 0.001). Conclusion: Significant cardiac haemodynamic alterations occurred during the newborn and early infancy periods and were detected by tissue Doppler, strain, and strain rate echocardiography. Although two-dimensional speckle-tracking echocardiography is useful and can produce improved, reliable results in clinical practice, it has some limitations. Therefore, more studies on this issue are required.
MeSH term(s)	Echocardiography, Doppler ; Echocardiography, Doppler, Pulsed ; Female ; Heart Ventricles/diagnostic imaging ; Humans ; Infant ; Infant, Newborn ; Infant, Premature ; Male ; Mitral Valve/diagnostic imaging ; Reference Values ; Systole/physiology ; Ventricular Function/physiology
Language	English
Publishing date	2014-04
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1078466-4
ISSN	1467-1107 ; 1047-9511
ISSN (online)	1467-1107
ISSN	1047-9511
DOI	10.1017/S1047951112002284
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