Article ; Online: Evaluation of Abbott anti-SARS-CoV-2 CMIA IgG and Euroimmun ELISA IgG/IgA assays in a clinical lab.
Clinica chimica acta; international journal of clinical chemistry
2020 Volume 510, Page(s) 687–690
Abstract: Background: We report our findings of test performance especially specificity of a fully automated Abbott Architect anti-SARS-CoV-2 CMIA IgG and Euroimmun anti-SARS-CoV-2 ELISA IgA/IgG in human plasma.: Methods: We used positive cohort of 97 samples ... ...
| Abstract | Background: We report our findings of test performance especially specificity of a fully automated Abbott Architect anti-SARS-CoV-2 CMIA IgG and Euroimmun anti-SARS-CoV-2 ELISA IgA/IgG in human plasma. Methods: We used positive cohort of 97 samples from Covid-19 patients or healthcare workers, collected at late time points from symptom onsets. We also included another cohort of 215 samples as negative controls, 78 of which had positive serology test results of other infectious diseases or autoimmunity. Assay specificity was assessed by using a total of 847 anonymized samples which were collected before the Covid-19 pandemic from local patient populations seeking clinical care for rheumatoid diseases, thyroid cancer, and therapeutic drug monitoring. Results: Abbott IgG, Euroimmun IgG/IgA had high precision, demonstrated by both intra- and inter-day CVs of <2%. There was no Abbott or Euroimmun IgG assay cross reactivity in the 78 samples with positive serology of non-SARS-CoV-2 infectious diseases and positive autoimmune antibodies. The Abbott IgG has specificity of 99.6%, while Euroimmun IgG and IgA were as high as 91.5% and 71.5%, respectively. Conclusions: Our evaluation confirmed high specificity of the Abbott IgG assay, while it was lower for Euroimmun IgG. Euroimmun IgA has suboptimal specificity which may limit its clinical use. Assay sensitivity was high for both Abbott and Euroimmun IgG assays. |
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| MeSH term(s) | Betacoronavirus/immunology ; Enzyme-Linked Immunosorbent Assay/methods ; Humans ; Immunoglobulin A/blood ; Immunoglobulin A/immunology ; Immunoglobulin G/blood ; Immunoglobulin G/immunology ; Limit of Detection ; Luminescent Measurements ; SARS-CoV-2 |
| Chemical Substances | Immunoglobulin A ; Immunoglobulin G |
| Keywords | covid19 |
| Language | English |
| Publishing date | 2020-09-08 |
| Publishing country | Netherlands |
| Document type | Evaluation Study ; Journal Article |
| ZDB-ID | 80228-1 |
| ISSN | 1873-3492 ; 0009-8981 |
| ISSN (online) | 1873-3492 |
| ISSN | 0009-8981 |
| DOI | 10.1016/j.cca.2020.09.002 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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