Article ; Online: Evaluation of bioaerosol samplers for the detection and quantification of influenza virus from artificial aerosols and influenza virus-infected ferrets.
Influenza and other respiratory viruses
2019 Volume 13, Issue 6, Page(s) 564–573
Abstract: Background: Bioaerosol sampling devices are necessary for the characterization of infectious bioaerosols emitted by naturally-infected hosts with acute respiratory virus infections. Assessment of these devices under multiple experimental conditions will ...
Abstract | Background: Bioaerosol sampling devices are necessary for the characterization of infectious bioaerosols emitted by naturally-infected hosts with acute respiratory virus infections. Assessment of these devices under multiple experimental conditions will provide insight for device use. Objectives: The primary objective of this study was to assess and compare bioaerosol sampling devices using a) an in vitro, environmentally-controlled artificial bioaerosol system at a range of different RH conditions and b) an in vivo bioaerosol system of influenza virus-infected ferrets under controlled environmental conditions. Secondarily, we also sought to examine the impact of NSAIDs on bioaerosol emission in influenza virus-infected ferrets to address its potential as a determinant of bioaerosol emission. Methods: We examined the performance of low and moderate volume bioaerosol samplers for the collection of viral RNA and infectious influenza virus in vitroand in vivo using artificial bioaerosols and the ferret model of influenza virus infection. The following samplers were tested: the polytetrafluoroethylene filter (PTFE filter), the 2-stage National Institute of Occupational Safety and Health cyclone sampler (NIOSH cyclone sampler) and the 6-stage viable Andersen impactor (Andersen impactor). Results: The PTFE filter and NIOSH cyclone sampler collected similar amounts of viral RNA and infectious virus from artificially-generated aerosols under a range of relative humidities (RH). Using the ferret model, the PTFE filter, NIOSH cyclone sampler and the Andersen impactor collected up to 3.66 log Conclusion: The PTFE filter and NIOSH cyclone sampler are useful for influenza virus RNA and infectious virus collection and may be considered for clinical and environmental settings. |
---|---|
MeSH term(s) | Aerosols ; Air Microbiology ; Animals ; Anti-Inflammatory Agents, Non-Steroidal/therapeutic use ; Disease Models, Animal ; Ferrets ; Humidity ; Influenza A virus/genetics ; Influenza A virus/isolation & purification ; Orthomyxoviridae Infections/drug therapy ; Orthomyxoviridae Infections/transmission ; Orthomyxoviridae Infections/virology ; Particle Size ; RNA, Viral/analysis ; Specimen Handling/instrumentation |
Chemical Substances | Aerosols ; Anti-Inflammatory Agents, Non-Steroidal ; RNA, Viral |
Keywords | covid19 |
Language | English |
Publishing date | 2019-09-21 |
Publishing country | England |
Document type | Comparative Study ; Evaluation Study ; Journal Article |
ZDB-ID | 2274538-5 |
ISSN | 1750-2659 ; 1750-2640 |
ISSN (online) | 1750-2659 |
ISSN | 1750-2640 |
DOI | 10.1111/irv.12678 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
Zs.A 6568: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.