LIVIVO - Search results -

Search results

Result 1 - 10 of total 16

Search options

Article ; Online: Estimating potential farm economic benefits of advanced microbiological diagnostics in veterinary medicine

Jensen, Jørgen Dejgård / Christensen, Tove / Pansri, Potjamas / Olsen, John Elmerdahl

Animal Production Science. 2023, v. 63, no. 15 p.1545-1558

2023

Abstract: Context Intensive livestock production is challenged by frequent occurrence of contagious livestock diseases. Advanced diagnostic tools, such as quantitative polymerase chain reaction (qPCR), have been found to improve the precision and speed of ... ...

Abstract	Context Intensive livestock production is challenged by frequent occurrence of contagious livestock diseases. Advanced diagnostic tools, such as quantitative polymerase chain reaction (qPCR), have been found to improve the precision and speed of veterinary diagnostics. Aims This study develops and applies an analytical quantitative framework to investigate the potential farm economic benefits of introducing an advanced and rapid diagnostic tool that allows veterinarians accurately and fast to detect the load and composition of pathogens in production animals, compared with a scenario where decision-making is based on aggregate prevalence data. Methods A probabilistic budget simulation model for livestock production was developed, on the basis of farm-accounts data, epidemiological prevalence, mortality and morbidity data from official statistics, veterinary practice and literature findings, as well as experimental data regarding sensitivity and specificity of the specific diagnostic tool. Key results The framework was used to assess the expected economic gains of qPCR diagnostics for calf pneumonia and weaner pig diarrhoea. In both cases, positive economic gains were found, namely, 7.8% and 3.1% of gross margin in Danish calf production and weaner production respectively. Conclusions and Implications Use of rapid advanced diagnostic tools to diagnose calf pneumonia or weaner pig diarrhoea can lead to economic gains for farmers and improve the efficiency in use of resources in livestock production.
Keywords	calves ; decision making ; diarrhea ; farms ; livestock production ; mortality ; pneumonia ; profits and margins ; quantitative polymerase chain reaction ; simulation models ; statistics ; swine ; veterinary clinics ; weanlings ; calf pneumomia ; economic benefits ; livestock ; probabilistic simulation ; qPCR ; uncertainty reduction ; veterinary diagnostics ; weaner pig diarrhoea
Language	English
Size	p. 1545-1558.
Publishing place	CSIRO Publishing
Document type	Article ; Online
ZDB-ID	2472524-9
ISSN	1836-5787 ; 1836-0939
ISSN (online)	1836-5787
ISSN	1836-0939
DOI	10.1071/AN22413
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 1435: Show issues

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Evaluation of a novel multiplex qPCR method for rapid detection and quantification of pathogens associated with calf diarrhoea.

Pansri, Potjamas / Svensmark, Birgitta / Liu, Gang / Thamsborg, Stig Milan / Kudirkiene, Egle / Nielsen, Henrik Vedel / Goecke, Nicole Bakkegård / Olsen, John Elmerdahl

Journal of applied microbiology

2022 Volume 133, Issue 4, Page(s) 2516–2527

Abstract: Aims: Diarrhoea is a common health problem in calves and a main reason for use of antimicrobials. It is associated with several bacterial, viral and parasitic pathogens, most of which are commonly present in healthy animals. Methods, which quantify the ... ...

Abstract	Aims: Diarrhoea is a common health problem in calves and a main reason for use of antimicrobials. It is associated with several bacterial, viral and parasitic pathogens, most of which are commonly present in healthy animals. Methods, which quantify the causative agents, may therefore improve confidence in associating a pathogen to the disease. This study evaluated a novel commercially available, multiplex quantitative polymerase chain reaction (qPCR) assay (Enterit4Calves) for detection and quantification of pathogens associated with calf-diarrhoea. Methods and results: Performance of the method was first evaluated under laboratory conditions. Then it was compared with current routine methods for detection of pathogens in faecal samples from 65 calves with diarrhoea and in 30 spiked faecal samples. The qPCR efficiencies were between 84%-103% and detection limits of 100-1000 copies of nucleic acids per sample were observed. Correct identification was obtained on 42 strains of cultured target bacteria, with only one false positive reaction from 135 nontarget bacteria. Kappa values for agreement between the novel assay and current routine methods varied between 0.38 and 0.83. Conclusion: The novel qPCR method showed good performance under laboratory conditions and a fair to good agreement with current routine methods when used for testing of field samples. Significance and impact of study: In addition to having fair to good detection abilities, the novel qPCR method allowed quantification of pathogens. In the future, use of quantification may improve diagnosis and hence treatment of calf diarrhoea.
MeSH term(s)	Animals ; Bacteria/genetics ; Cattle ; Diarrhea/diagnosis ; Diarrhea/microbiology ; Diarrhea/veterinary ; Feces/microbiology ; Multiplex Polymerase Chain Reaction/methods ; Nucleic Acids ; Sensitivity and Specificity
Chemical Substances	Nucleic Acids
Language	English
Publishing date	2022-07-31
Publishing country	England
Document type	Journal Article
ZDB-ID	1358023-1
ISSN	1365-2672 ; 1364-5072
ISSN (online)	1365-2672
ISSN	1364-5072
DOI	10.1111/jam.15722
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 259: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Evaluation of a novel multiplex qPCR method for rapid detection and quantification of pathogens associated with calf diarrhoea

Pansri, Potjamas / Svensmark, Birgitta / Liu, Gang / Thamsborg, Stig Milan / Kudirkiene, Egle / Nielsen, Henrik Vedel / Goecke, Nicole Bakkegård / Olsen, John Elmerdahl

Journal of applied microbiology. 2022 Oct., v. 133, no. 4

2022

Abstract: AIMS: Diarrhoea is a common health problem in calves and a main reason for use of antimicrobials. It is associated with several bacterial, viral and parasitic pathogens, most of which are commonly present in healthy animals. Methods, which quantify the ... ...

Abstract	AIMS: Diarrhoea is a common health problem in calves and a main reason for use of antimicrobials. It is associated with several bacterial, viral and parasitic pathogens, most of which are commonly present in healthy animals. Methods, which quantify the causative agents, may therefore improve confidence in associating a pathogen to the disease. This study evaluated a novel commercially available, multiplex quantitative polymerase chain reaction (qPCR) assay (Enterit4Calves) for detection and quantification of pathogens associated with calf‐diarrhoea. METHODS AND RESULTS: Performance of the method was first evaluated under laboratory conditions. Then it was compared with current routine methods for detection of pathogens in faecal samples from 65 calves with diarrhoea and in 30 spiked faecal samples. The qPCR efficiencies were between 84%–103% and detection limits of 100–1000 copies of nucleic acids per sample were observed. Correct identification was obtained on 42 strains of cultured target bacteria, with only one false positive reaction from 135 nontarget bacteria. Kappa values for agreement between the novel assay and current routine methods varied between 0.38 and 0.83. CONCLUSION: The novel qPCR method showed good performance under laboratory conditions and a fair to good agreement with current routine methods when used for testing of field samples. SIGNIFICANCE AND IMPACT OF STUDY: In addition to having fair to good detection abilities, the novel qPCR method allowed quantification of pathogens. In the future, use of quantification may improve diagnosis and hence treatment of calf diarrhoea.
Keywords	anti-infective agents ; calves ; diarrhea ; microbiology ; pathogens ; quantitative polymerase chain reaction ; rapid methods
Language	English
Dates of publication	2022-10
Size	p. 2516-2527.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	1358023-1
ISSN	1365-2672 ; 1364-5072
ISSN (online)	1365-2672
ISSN	1364-5072
DOI	10.1111/jam.15722
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 259: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Brain-derived neurotrophic factor increases cell number of neural progenitor cells derived from human induced pluripotent stem cells.

Pansri, Panetha / Phanthong, Phetcharat / Suthprasertporn, Nopparat / Kitiyanant, Yindee / Tubsuwan, Alisa / Dinnyes, Andras / Kobolak, Julianna / Kitiyanant, Narisorn

PeerJ

2021 Volume 9, Page(s) e11388

Abstract: Background: Several pieces of evidence from in vitro studies showed that brain-derived neurotrophic factor (BDNF) promotes proliferation and differentiation of neural stem/progenitor cells (NSCs) into neurons. Moreover, the JAK2 pathway was proposed to ... ...

Abstract	Background: Several pieces of evidence from in vitro studies showed that brain-derived neurotrophic factor (BDNF) promotes proliferation and differentiation of neural stem/progenitor cells (NSCs) into neurons. Moreover, the JAK2 pathway was proposed to be associated with mouse NSC proliferation. BDNF could activate the STAT-3 pathway and induce proliferation in mouse NSCs. However, its effects on proliferation are not fully understood and JAK/STAT pathway was proposed to play a role in this activity. Methods: In the present study, the effects of BDNF on cell proliferation and neurite outgrowth of Alzheimer's disease (AD) induced pluripotent stem cells (iPSCs)-derived human neural progenitor cells (hNPCs) were examined. Moreover, a specific signal transduction pathway important in cell proliferation was investigated using a JAK2 inhibitor (AG490) to clarify the role of that pathway. Results: The proliferative effect of BDNF was remarkably observed as an increase in Ki-67 positive cells. The cell number of hNPCs was significantly increased after BDNF treatment represented by cellular metabolic activity of the cells measured by MTT assay. This noticeable effect was statistically shown at 20 ng/ml of BDNF treatment. BDNF, however, did not promote neurite outgrowth but increased neuronal cell number. It was found that AG490 suppressed hNPCs proliferation. However, this inhibitor partially decreased BDNF-induced hNPCs proliferation. These results demonstrated the potential role of BDNF for the amelioration of AD through the increase of AD-derived hNPCs number.
Language	English
Publishing date	2021-05-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	2703241-3
ISSN	2167-8359
ISSN	2167-8359
DOI	10.7717/peerj.11388
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Occurrence of major and minor pathogens in calves diagnosed with bovine respiratory disease.

Kudirkiene, Egle / Aagaard, Anne Katrine / Schmidt, Louise M B / Pansri, Potjamas / Krogh, Kenneth M / Olsen, John E

Veterinary microbiology

2021 Volume 259, Page(s) 109135

Abstract: Bovine respiratory disease (BRD) is caused by a mixture of viruses and opportunistic bacteria belonging to Pasteurellaceae and Mycoplasma bovis. However, these organisms are also commonly isolated from healthy calves. This study aimed to determine ... ...

Abstract	Bovine respiratory disease (BRD) is caused by a mixture of viruses and opportunistic bacteria belonging to Pasteurellaceae and Mycoplasma bovis. However, these organisms are also commonly isolated from healthy calves. This study aimed to determine whether the organisms are present in higher numbers in calves sick with acute BRD than in clinically healthy calves, and further to genetically characterize bacteria of the family Pasteurellaceae to understand whether particular types are associated with disease. Forty-six clinically healthy and 46 calves with BRD were sampled by broncheoalveolar lavage (BAL) method in 11 herds geographically spread over Denmark to determine presence and quantity of microorganisms by culture and quantitative real time qPCR. Isolates of Pasteurellaceae were tested for antibiotic resistance and were whole genome sequenced to determine genotypes. Histophilus somni was in particular positively associated with BRD, suggesting particular importance of this organism as likely aetiology of BRD. In addition, quantification of bacteria revealed that higher counts of H. somni as well as of M. haemolytica was also a good indicator of the disease. Pasteurellaceae isolates were susceptible to the commonly used antibiotics in treatment of BRD, and genotypes were shared between isolates from clinically healthy and sick calves.
MeSH term(s)	Animals ; Anti-Bacterial Agents/pharmacology ; Bacteria/classification ; Bacteria/genetics ; Bacteria/isolation & purification ; Bacteria/pathogenicity ; Bovine Respiratory Disease Complex/microbiology ; Bronchoalveolar Lavage Fluid/microbiology ; Bronchoalveolar Lavage Fluid/virology ; Cattle ; Cattle Diseases/virology ; Mannheimia haemolytica/genetics ; Mannheimia haemolytica/isolation & purification ; Mannheimia haemolytica/pathogenicity ; Pasteurellaceae/classification ; Pasteurellaceae/drug effects ; Pasteurellaceae/genetics ; Pasteurellaceae/pathogenicity ; Respiratory Tract Diseases/microbiology ; Respiratory Tract Diseases/veterinary ; Respiratory Tract Diseases/virology
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2021-05-27
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	753154-0
ISSN	1873-2542 ; 0378-1135
ISSN (online)	1873-2542
ISSN	0378-1135
DOI	10.1016/j.vetmic.2021.109135
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2917: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Evaluation of novel multiplex qPCR assays for diagnosis of pathogens associated with the bovine respiratory disease complex.

Pansri, P / Katholm, J / Krogh, K M / Aagaard, A K / Schmidt, L M B / Kudirkiene, E / Larsen, L E / Olsen, J E

Veterinary journal (London, England : 1997)

2020 Volume 256, Page(s) 105425

Abstract: Bovine respiratory disease complex is the most common disease requiring the use of antimicrobials in industrial calf production worldwide. Pathogenic bacteria (Mannheimia haemolytica (Mh), Pasteurella multocida (Pm), Histophilus somni (Hs), and ... ...

Abstract	Bovine respiratory disease complex is the most common disease requiring the use of antimicrobials in industrial calf production worldwide. Pathogenic bacteria (Mannheimia haemolytica (Mh), Pasteurella multocida (Pm), Histophilus somni (Hs), and Mycoplasma bovis) and a range of viruses (bovine respiratory syncytial virus, bovine coronavirus, bovine parainfluenza virus type 3, bovine viral diarrhea virus and bovine herpesvirus type 1) are associated with this complex. As most of these pathogens can be present in healthy and diseased calves, simple detection of their presence in diseased calves carries low predictive value. In other multi-agent diseases of livestock, quantification of pathogens has added substantially to the predictive value of microbiological diagnosis. The aim of this study was to evaluate the ability of two recently developed quantitative PCR (qPCR) kits (Pneumo4B and Pneumo4V) to detect and quantify these bacterial and viral pathogens, respectively. Test efficiencies of the qPCR assays, based on nucleic acid dilution series of target bacteria and viruses, were 93-106% and 91-104%, respectively, with assay detection limits of 10-50 copies of nucleic acids. All 44 strains of target bacteria were correctly identified, with no false positive reactions in 135strains of non-target bacterial species. Based on standard curves of log
MeSH term(s)	Animals ; Bacteria/genetics ; Bacteria/isolation & purification ; Bovine Respiratory Disease Complex/diagnosis ; Bovine Respiratory Disease Complex/microbiology ; Bovine Respiratory Disease Complex/virology ; Cattle ; Multiplex Polymerase Chain Reaction/methods ; Multiplex Polymerase Chain Reaction/veterinary ; Sensitivity and Specificity ; Viruses/genetics ; Viruses/isolation & purification
Keywords	covid19
Language	English
Publishing date	2020-01-16
Publishing country	England
Document type	Evaluation Study ; Journal Article
ZDB-ID	428614-5
ISSN	1532-2971 ; 0372-5545 ; 1090-0233
ISSN (online)	1532-2971
ISSN	0372-5545 ; 1090-0233
DOI	10.1016/j.tvjl.2020.105425
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 552: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Evaluation of novel multiplex qPCR assays for diagnosis of pathogens associated with the bovine respiratory disease complex

Pansri, P / Katholm, J / Krogh, K.M / Aagaard, A.K / Schmidt, L.M.B / Kudirkiene, E / Larsen, L.E / Olsen, J.E

veterinary journal. 2020 Feb., v. 256

2020

Abstract	Bovine respiratory disease complex is the most common disease requiring the use of antimicrobials in industrial calf production worldwide. Pathogenic bacteria (Mannheimia haemolytica (Mh), Pasteurella multocida (Pm), Histophilus somni (Hs), and Mycoplasma bovis) and a range of viruses (bovine respiratory syncytial virus, bovine coronavirus, bovine parainfluenza virus type 3, bovine viral diarrhea virus and bovine herpesvirus type 1) are associated with this complex. As most of these pathogens can be present in healthy and diseased calves, simple detection of their presence in diseased calves carries low predictive value. In other multi-agent diseases of livestock, quantification of pathogens has added substantially to the predictive value of microbiological diagnosis. The aim of this study was to evaluate the ability of two recently developed quantitative PCR (qPCR) kits (Pneumo4B and Pneumo4V) to detect and quantify these bacterial and viral pathogens, respectively.Test efficiencies of the qPCR assays, based on nucleic acid dilution series of target bacteria and viruses, were 93–106% and 91–104%, respectively, with assay detection limits of 10–50 copies of nucleic acids. All 44 strains of target bacteria were correctly identified, with no false positive reactions in 135strains of non-target bacterial species. Based on standard curves of log10 CFU versus cycle threshold (Ct) values, quantification was possible over a 5-log range of bacteria. In 92 tracheal aspirate samples, the kappa values for agreement between Pneumo4B and bacterial culture were 0.64-0.84 for Mh, Pm and Hs. In an additional 84 tracheal aspirates, agreement between Pneumo4B or Pneumo 4V and certified diagnostic qPCR assays was moderate (0.57) for M. bovis and high (0.71–0.90) for viral pathogens. Thus Pneumo4 kits specifically detected and quantified the relevant pathogens.
Keywords	Bovine alphaherpesvirus 1 ; Bovine orthopneumovirus ; Bovine respirovirus 3 ; Bovine viral diarrhea virus 1 ; Haemophilus somni ; Mannheimia haemolytica ; Mycoplasma bovis ; Pasteurella multocida ; animal viruses ; anti-infective agents ; bacteria ; bacterial culture ; bovine respiratory disease ; calves ; detection limit ; livestock diseases ; nucleic acids ; quantitative polymerase chain reaction ; virulent strains ; viruses ; covid19
Language	English
Dates of publication	2020-02
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	428614-5
ISSN	1532-2971 ; 0372-5545 ; 1090-0233
ISSN (online)	1532-2971
ISSN	0372-5545 ; 1090-0233
DOI	10.1016/j.tvjl.2020.105425
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 3664: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article: The CT appearances for differentiating of peripheral, mass-forming cholangiocarcinoma and liver meatastases from colorectal adenocarcinoma.

Apisarnthanarak, Piyaporn / Pansri, Chalotorn / Maungsomboon, Kobkun / Thamtorawat, Somraj

Journal of the Medical Association of Thailand = Chotmaihet thangphaet

2014 Volume 97, Issue 4, Page(s) 415–422

Abstract: ... respectively. The mean size (SD) were 7.4 (3.7) cm vs. 4.0 (2.1) cm, in Group 1 and 2, respectively (p < 0.001 ... significantly higher in Group 1 than Group 2 (p < 0.001). In contrary, the presence of multiple lesions ... with separated locations, and smooth margin were significantly suggested of Group 2 (p < 0.001 and p = 0.007 ...

Abstract	Objective: To evaluate the computed tomographic (CT) appearances for differentiating of primary hepatic adenocarcinoma (peripheral, mass-forming cholangiocarcinoma) and secondary hepatic adenocarcinoma (liver metastases from colorectal carcinoma). Material and method: Between January 2004 and December 2010, 45 patients with peripheral, mass-forming cholangiocarcinoma (Group 1) and 45 patients with liver metastases from colorectal adenocarcinoma (Group 2) who underwent abdominal CT scan at the authors' institution were included in the present retrospective study. Two experienced, abdominal radiologists blinded to the participants 'clinical histories and pathological results, separately reviewed the CT findings of each participant (number of liver mass(es), size, margin, internal calcification, hepatic capsule retraction, vascular invasion, peripheral bile duct dilatation, proximal bile duct enhancement, extrahepatic spreading, nearby lymphadenopathy and nearby organ invasion) and gave the presumed diagnosis of each individual case. Any discrepancies were solved by a consensus review. Finally, the authors conducted a stratified analysis of the patients in both groups based on their CT appearances. Results: Ninety participants were 35 (38.9%) female, 55 (61.1%) male, age range from 43 to 88 years (mean 63.4 years, SD = 10.7). There were 28.9% vs. 48.9% female with the mean age (SD) of 61.5 (9.4) vs. 65.4 (11.6) years in Group 1 and 2, respectively. The mean size (SD) were 7.4 (3.7) cm vs. 4.0 (2.1) cm, in Group 1 and 2, respectively (p < 0.001). The presence of hepatic capsule retraction, vascular invasion, peripheral bile duct dilatation, proximal bile duct enhancement, extrahepatic spreading, nearby lymphadenopathy, and nearby organ invasion were significantly higher in Group 1 than Group 2 (p < 0.001). In contrary, the presence of multiple lesions with separated locations, and smooth margin were significantly suggested of Group 2 (p < 0.001 and p = 0.007, respectively). By logistic regression analysis, peripheral bile duct dilatation, extrahepatic spreading, and proximal bile duct enhancement were the sole predictors of peripheral, mass-forming cholangiocarcinoma. The interobserver agreement for the presumed diagnosis of liver mass was good (kappa = 0.76). Conclusion: The presence of peripheral bile duct dilatation, extrahepatic spreading, and proximal bile duct enhancement were highly suggestive of peripheral, mass-forming cholangiocarcinoma.
MeSH term(s)	Adenocarcinoma/diagnostic imaging ; Adenocarcinoma/secondary ; Adult ; Aged ; Aged, 80 and over ; Bile Duct Neoplasms ; Bile Ducts, Intrahepatic ; Cholangiocarcinoma/diagnostic imaging ; Cholangiocarcinoma/pathology ; Colorectal Neoplasms/pathology ; Diagnosis, Differential ; Female ; Humans ; Liver Neoplasms/diagnostic imaging ; Liver Neoplasms/pathology ; Liver Neoplasms/secondary ; Male ; Middle Aged ; Retrospective Studies ; Tomography, X-Ray Computed
Language	English
Publishing date	2014-04
Publishing country	Thailand
Document type	Journal Article
ZDB-ID	801630-6
ISSN	0125-2208 ; 0025-7036
ISSN	0125-2208 ; 0025-7036
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 957: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Brain-derived neurotrophic factor increases cell number of neural progenitor cells derived from human induced pluripotent stem cells

Panetha Pansri / Phetcharat Phanthong / Nopparat Suthprasertporn / Yindee Kitiyanant / Alisa Tubsuwan / Andras Dinnyes / Julianna Kobolak / Narisorn Kitiyanant

PeerJ, Vol 9, p e

2021 Volume 11388

Abstract: Background Several pieces of evidence from in vitro studies showed that brain-derived neurotrophic factor (BDNF) promotes proliferation and differentiation of neural stem/progenitor cells (NSCs) into neurons. Moreover, the JAK2 pathway was proposed to be ...

Abstract	Background Several pieces of evidence from in vitro studies showed that brain-derived neurotrophic factor (BDNF) promotes proliferation and differentiation of neural stem/progenitor cells (NSCs) into neurons. Moreover, the JAK2 pathway was proposed to be associated with mouse NSC proliferation. BDNF could activate the STAT-3 pathway and induce proliferation in mouse NSCs. However, its effects on proliferation are not fully understood and JAK/STAT pathway was proposed to play a role in this activity. Methods In the present study, the effects of BDNF on cell proliferation and neurite outgrowth of Alzheimer’s disease (AD) induced pluripotent stem cells (iPSCs)-derived human neural progenitor cells (hNPCs) were examined. Moreover, a specific signal transduction pathway important in cell proliferation was investigated using a JAK2 inhibitor (AG490) to clarify the role of that pathway. Results The proliferative effect of BDNF was remarkably observed as an increase in Ki-67 positive cells. The cell number of hNPCs was significantly increased after BDNF treatment represented by cellular metabolic activity of the cells measured by MTT assay. This noticeable effect was statistically shown at 20 ng/ml of BDNF treatment. BDNF, however, did not promote neurite outgrowth but increased neuronal cell number. It was found that AG490 suppressed hNPCs proliferation. However, this inhibitor partially decreased BDNF-induced hNPCs proliferation. These results demonstrated the potential role of BDNF for the amelioration of AD through the increase of AD-derived hNPCs number.
Keywords	Brain-derived neurotrophic factor ; Neural progenitor cells ; Induced pluripotent stem cells ; Proliferation ; Alzheimer’s disease ; Medicine ; R ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2021-05-01T00:00:00Z
Publisher	PeerJ Inc.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: A compact phage display human scFv library for selection of antibodies to a wide variety of antigens.

Pansri, Potjamas / Jaruseranee, Nanthnit / Rangnoi, Kuntalee / Kristensen, Peter / Yamabhai, Montarop

BMC biotechnology

2009 Volume 9, Page(s) 6

Abstract: Background: Phage display technology is a powerful new tool for making antibodies outside the immune system, thus avoiding the use of experimental animals. In the early days, it was postulated that this technique would eventually replace hybridoma ... ...

Abstract	Background: Phage display technology is a powerful new tool for making antibodies outside the immune system, thus avoiding the use of experimental animals. In the early days, it was postulated that this technique would eventually replace hybridoma technology and animal immunisations. However, since this technology emerged more than 20 years ago, there have only been a handful reports on the construction and application of phage display antibody libraries world-wide. Results: Here we report the simplest and highly efficient method for the construction of a highly useful human single chain variable fragment (scFv) library. The least number of oligonucleotide primers, electroporations and ligation reactions were used to generate a library of 1.5 x 108 individual clones, without generation of sub-libraries. All possible combinations of heavy and light chains, among all immunoglobulin isotypes, were included by using a mixture of primers and overlapping extension PCR. The key difference from other similar libraries was the highest diversity of variable gene repertoires, which was derived from 140 non-immunized human donors. A wide variety of antigens were successfully used to affinity select specific binders. These included pure recombinant proteins, a hapten and complex antigens such as viral coat proteins, crude snake venom and cancer cell surface antigens. In particular, we were able to use standard bio-panning method to isolate antibody that can bind to soluble Aflatoxin B1, when using BSA-conjugated toxin as a target, as demonstrated by inhibition ELISA. Conclusion: These results suggested that by using an optimized protocol and very high repertoire diversity, a compact and efficient phage antibody library can be generated. This advanced method could be adopted by any molecular biology laboratory to generate both naïve or immunized libraries for particular targets as well as for high-throughput applications.
MeSH term(s)	Cloning, Molecular ; DNA Fingerprinting ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunoglobulin Fragments/genetics ; Immunoglobulin Fragments/immunology ; Immunoglobulin Variable Region/genetics ; Immunoglobulin Variable Region/immunology ; Peptide Library ; Recombinant Proteins/genetics ; Recombinant Proteins/immunology ; Sensitivity and Specificity ; Sequence Analysis, DNA
Chemical Substances	Immunoglobulin Fragments ; Immunoglobulin Variable Region ; Peptide Library ; Recombinant Proteins
Language	English
Publishing date	2009-01-29
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1472-6750
ISSN (online)	1472-6750
DOI	10.1186/1472-6750-9-6
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED