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Article ; Online: Wearable Biosensors in the Workplace: Perceptions and Perspectives.

Tindale, Lauren C / Chiu, Derek / Minielly, Nicole / Hrincu, Viorica / Talhouk, Aline / Illes, Judy

Frontiers in digital health

2022 Volume 4, Page(s) 800367

Abstract: Objectives: Wearable body and brain sensors are permeating the consumer market and are increasingly being considered for workplace applications with the goal of promoting safety, productivity, health, and wellness. However, the monitoring of physiologic ...

Abstract	Objectives: Wearable body and brain sensors are permeating the consumer market and are increasingly being considered for workplace applications with the goal of promoting safety, productivity, health, and wellness. However, the monitoring of physiologic signals in real-time prompts concerns about benefit and risk, ownership of such digital data, data transfer privacy, and the discovery and disclosure of signals of possible health significance. Here we explore the perceptions and perspectives of employers and employees about key ethical considerations regarding the potential use of sensors in the workplace. Methods: We distributed a survey developed and refined based on key research questions and past literature to a wide range and size of industries in British Columbia, Canada. Both employers (potential Implementers) and employees (potential Users) were invited to participate. Results: We received 344 survey responses. Most responses were from construction, healthcare, education, government, and utilities sectors. Across genders, industries, and workplace sizes, we found a convergence of opinions on perceived benefit and concern between potential Implementers and potential Users regarding the motivation to use biosensors in the workplace. Potential Implementers and Users also agreed on issues pertaining to safety, privacy, disclosure of findings of possible medical significance, risks, data ownership, data sharing, and transfer of data between workplaces. The greatest variability between potential Users and Implementers pertained to data ownership. Conclusion: Strong agreement in the perception of biosensor use in the workplace between potential Implementers and Users reflects shared interest, motivation, and responsibility for their use. The use of sensors is rapidly increasing, and transparency about key use factors-both practical and ethical-is essential to maintain the current and desirable level of solidarity.
Language	English
Publishing date	2022-07-04
Publishing country	Switzerland
Document type	Journal Article
ISSN	2673-253X
ISSN (online)	2673-253X
DOI	10.3389/fdgth.2022.800367
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Article ; Online: 10-year follow-up of the Super-Seniors Study: compression of morbidity and genetic factors.

Tindale, Lauren C / Salema, Diane / Brooks-Wilson, Angela R

BMC geriatrics

2019 Volume 19, Issue 1, Page(s) 58

Abstract: Background: Super-Seniors are healthy, long-lived individuals who were recruited at age 85 years or older with no history of cancer, cardiovascular disease, diabetes, dementia, or major pulmonary disease. In a 10-year follow-up, we aimed to determine ... ...

Abstract	Background: Super-Seniors are healthy, long-lived individuals who were recruited at age 85 years or older with no history of cancer, cardiovascular disease, diabetes, dementia, or major pulmonary disease. In a 10-year follow-up, we aimed to determine whether surviving Super-Seniors showed compression of morbidity, and to test whether the allele frequencies of longevity-associated variants in APOE and FOXO3 were more extreme in such long-term survivors. Methods: Super-Seniors who survived and were contactable were re-interviewed 10 years after initial characterization. Health and lifestyle were characterized via questionnaire. Geriatric tests including the Timed Up and Go (TUG), Geriatric Depression Scale (GDS), Instrumental Activities of Daily Living (IADL) and the Mini-Mental State Exam (MMSE) were administered, and data were compared to results from on average 10 years earlier. As well, genotype and allele frequencies for SNPs rs7412 and rs429358 in APOE, and rs2802292 in FOXO3 were compared to the frequencies in the original collection of Super-Seniors and mid-life controls. Results: Of the 480 Super-Seniors recruited from 2004 to 2007, 13 were alive, contactable, and consented to re-interview (mean age = 100.1 ± 3.3). Eight of these 13 participants (62%) still met Super-Senior health criteria. Diseases that occurred in late life were cardiovascular (5 of 13; 38%) and lung disease (1 of 13; 8%). MMSE and IADL scores declined in the decade between interviews, and GDS and TUG scores increased. The surviving group of centenarians had a higher frequency of APOE and FOXO3 longevity-associated variants even when compared to the original long-lived Super-Senior cohort. Conclusions: Although physical and mental decline occurred in the decade between interviews, the majority of Super-Seniors re-interviewed still met the original health criteria. These observations are consistent with reports of compression of morbidity at extreme ages, particularly in centenarians. The increased frequency of longevity- associated variants in this small group of survivors is consistent with studies that reported genetics as a larger contributor to longevity in older age groups.
MeSH term(s)	Activities of Daily Living/psychology ; Aged, 80 and over ; Aging/genetics ; Aging/psychology ; Dementia/diagnosis ; Dementia/genetics ; Female ; Follow-Up Studies ; Genotype ; Humans ; Life Style ; Longevity/genetics ; Male ; Morbidity ; Polymorphism, Single Nucleotide/genetics ; Surveys and Questionnaires ; Time Factors
Language	English
Publishing date	2019-02-28
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2059865-8
ISSN	1471-2318 ; 1471-2318
ISSN (online)	1471-2318
ISSN	1471-2318
DOI	10.1186/s12877-019-1080-8
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Article ; Online: Wearable Biosensors in the Workplace

Lauren C. Tindale / Derek Chiu / Nicole Minielly / Viorica Hrincu / Aline Talhouk / Judy Illes

Frontiers in Digital Health, Vol

Perceptions and Perspectives

2022 Volume 4

Abstract: ObjectivesWearable body and brain sensors are permeating the consumer market and are increasingly being considered for workplace applications with the goal of promoting safety, productivity, health, and wellness. However, the monitoring of physiologic ... ...

Abstract	ObjectivesWearable body and brain sensors are permeating the consumer market and are increasingly being considered for workplace applications with the goal of promoting safety, productivity, health, and wellness. However, the monitoring of physiologic signals in real-time prompts concerns about benefit and risk, ownership of such digital data, data transfer privacy, and the discovery and disclosure of signals of possible health significance. Here we explore the perceptions and perspectives of employers and employees about key ethical considerations regarding the potential use of sensors in the workplace.MethodsWe distributed a survey developed and refined based on key research questions and past literature to a wide range and size of industries in British Columbia, Canada. Both employers (potential Implementers) and employees (potential Users) were invited to participate.ResultsWe received 344 survey responses. Most responses were from construction, healthcare, education, government, and utilities sectors. Across genders, industries, and workplace sizes, we found a convergence of opinions on perceived benefit and concern between potential Implementers and potential Users regarding the motivation to use biosensors in the workplace. Potential Implementers and Users also agreed on issues pertaining to safety, privacy, disclosure of findings of possible medical significance, risks, data ownership, data sharing, and transfer of data between workplaces. The greatest variability between potential Users and Implementers pertained to data ownership.ConclusionStrong agreement in the perception of biosensor use in the workplace between potential Implementers and Users reflects shared interest, motivation, and responsibility for their use. The use of sensors is rapidly increasing, and transparency about key use factors–both practical and ethical–is essential to maintain the current and desirable level of solidarity.
Keywords	wearable sensor ; wearable electronic devices ; occupational safety ; biosensor ; corporate ethics ; workplace sensor ; Medicine ; R ; Public aspects of medicine ; RA1-1270 ; Electronic computers. Computer science ; QA75.5-76.95
Subject code	170
Language	English
Publishing date	2022-07-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
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Article ; Online: Allele-Specific Transcript Abundance: A Pilot Study in Healthy Centenarians.

Tindale, Lauren C / Thiessen, Nina / Leach, Stephen / Brooks-Wilson, Angela R

The journals of gerontology. Series A, Biological sciences and medical sciences

2019 Volume 75, Issue 6, Page(s) 1068–1072

Abstract: The genetic basis of healthy aging and longevity remains largely unexplained. One hypothesis as to why long-lived individuals do not appear to have a lower number of common-complex disease variants, is that despite carrying risk variants, they express ... ...

Abstract	The genetic basis of healthy aging and longevity remains largely unexplained. One hypothesis as to why long-lived individuals do not appear to have a lower number of common-complex disease variants, is that despite carrying risk variants, they express disease-linked alleles at a lower level than the wild-type alleles. Allele-specific abundance (ASA) is the different transcript abundance of the two haplotypes of a diploid individual. We sequenced the transcriptomes of four healthy centenarians and four mid-life controls. CIBERSORT was used to estimate blood cell fractions: neutrophils were the most abundant source of RNA, followed by CD8+ T cells, resting NK cells, and monocytes. ASA variants were more common in noncoding than coding regions. Centenarians and controls had a comparable distribution of ASA variants by predicted effect, and we did not observe an overall bias in expression toward major or minor alleles. Immune pathways were most highly represented among the gene set that showed ASA. Although we found evidence of ASA in disease-associated genes and transcription factors, we did not observe any differences in the pattern of expression between centenarians and controls in this small pilot study.
MeSH term(s)	Aged, 80 and over ; Alleles ; Diploidy ; Female ; Gene Expression Profiling ; Genes/genetics ; Genetic Predisposition to Disease/genetics ; Genetic Variation/genetics ; Haplotypes/genetics ; Healthy Aging/genetics ; Humans ; Male ; Pilot Projects ; Transcriptome/genetics
Language	English
Publishing date	2019-09-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1223643-3
ISSN	1758-535X ; 1079-5006
ISSN (online)	1758-535X
ISSN	1079-5006
DOI	10.1093/gerona/glz188
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Article ; Online: Gynecologic Cancer Risk and Genetics: Informing an Ideal Model of Gynecologic Cancer Prevention.

Tindale, Lauren C / Zhantuyakova, Almira / Lam, Stephanie / Woo, Michelle / Kwon, Janice S / Hanley, Gillian E / Knoppers, Bartha / Schrader, Kasmintan A / Peacock, Stuart J / Talhouk, Aline / Dummer, Trevor / Metcalfe, Kelly / Pashayan, Nora / Foulkes, William D / Manchanda, Ranjit / Huntsman, David / Stuart, Gavin / Simard, Jacques / Dawson, Lesa

Current oncology (Toronto, Ont.)

2022 Volume 29, Issue 7, Page(s) 4632–4646

Abstract: Individuals with proven hereditary cancer syndrome (HCS) such ... ...

Abstract	Individuals with proven hereditary cancer syndrome (HCS) such as
MeSH term(s)	British Columbia ; Female ; Genetic Predisposition to Disease ; Genetic Testing ; Genital Neoplasms, Female/diagnosis ; Genital Neoplasms, Female/genetics ; Genital Neoplasms, Female/prevention & control ; Humans ; Risk
Language	English
Publishing date	2022-06-30
Publishing country	Switzerland
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	1236972-x
ISSN	1718-7729 ; 1198-0052
ISSN (online)	1718-7729
ISSN	1198-0052
DOI	10.3390/curroncol29070368
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Article ; Online: Age-dependent metabolic dysregulation in cancer and Alzheimer's disease.

Harris, Richard A / Tindale, Lauren / Cumming, Robert C

Biogerontology

2014 Volume 15, Issue 6, Page(s) 559–577

Abstract: Age is the main risk factor for cancer and neurodegeneration; two radically divergent diseases. Yet selective pressure to meet cellular metabolic needs may provide a common mechanism linking these two disorders. The exclusive use of glycolysis, despite ... ...

Abstract	Age is the main risk factor for cancer and neurodegeneration; two radically divergent diseases. Yet selective pressure to meet cellular metabolic needs may provide a common mechanism linking these two disorders. The exclusive use of glycolysis, despite the presence of oxygen, is commonly referred to as aerobic glycolysis and is the primary metabolic pathway of cancer cells. Recent evidence suggests that aerobic glycolysis is also a key regulator of synaptic plasticity in the brain that may positively influence cognition. Elevated aerobic glycolysis is a contributing factor to the development of cancer as increased glycolytic flux plays an important role in the biosynthesis of macromolecules and promotes proliferation. In contrast, decreased aerobic glycolysis in the brain occurs with age and could lead to a loss of cell survival mechanisms that counter pathogenic processes underlying neurodegeneration. In this review we discuss the recent findings from epidemiological studies demonstrating an inverse comorbidity of cancer and Alzheimer's disease. We summarize evidence linking the two diseases through changes in metabolism over the course of normal aging. We discuss the key steps and regulatory mechanisms of aerobic glycolysis and mitochondrial oxidative phosphorylation which could be exploited for the development of novel therapies. In addition, we outline the regulation of aerobic glycolysis at the transcriptional level by HIF-1α and Pin1 and their roles in cancer and neurodegeneration. Finally, we provide a possible explanation for metabolic dysregulation that occurs with age, and how it may be a contributing factor to age-related diseases. Determining how metabolism becomes dysregulated over time could lead to the development of effective interventions for ensuring metabolic homeostasis and healthy aging.
MeSH term(s)	Aerobiosis ; Aged ; Aging/metabolism ; Alzheimer Disease/epidemiology ; Alzheimer Disease/metabolism ; Brain/metabolism ; Cellular Reprogramming ; Comorbidity ; Glucose Transport Proteins, Facilitative/metabolism ; Glycolysis ; Hexokinase/metabolism ; Humans ; Hypoxia-Inducible Factor 1/metabolism ; L-Lactate Dehydrogenase/metabolism ; NIMA-Interacting Peptidylprolyl Isomerase ; Neoplasms/epidemiology ; Neoplasms/metabolism ; Nerve Degeneration/metabolism ; Oxidative Phosphorylation ; Peptidylprolyl Isomerase/metabolism ; Pyruvate Kinase/metabolism ; Risk Factors
Chemical Substances	Glucose Transport Proteins, Facilitative ; Hypoxia-Inducible Factor 1 ; NIMA-Interacting Peptidylprolyl Isomerase ; L-Lactate Dehydrogenase (EC 1.1.1.27) ; Hexokinase (EC 2.7.1.1) ; Pyruvate Kinase (EC 2.7.1.40) ; PIN1 protein, human (EC 5.2.1.8) ; Peptidylprolyl Isomerase (EC 5.2.1.8)
Language	English
Publishing date	2014-12
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	2047160-9
ISSN	1573-6768 ; 1389-5729
ISSN (online)	1573-6768
ISSN	1389-5729
DOI	10.1007/s10522-014-9534-z
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Article ; Online: Lipid and Alzheimer's disease genes associated with healthy aging and longevity in healthy oldest-old.

Tindale, Lauren C / Leach, Stephen / Spinelli, John J / Brooks-Wilson, Angela R

Oncotarget

2017 Volume 8, Issue 13, Page(s) 20612–20621

Abstract: Several studies have found that long-lived individuals do not appear to carry lower numbers of common disease-associated variants than ordinary people; it has been hypothesized that they may instead carry protective variants. An intriguing type of ... ...

Abstract	Several studies have found that long-lived individuals do not appear to carry lower numbers of common disease-associated variants than ordinary people; it has been hypothesized that they may instead carry protective variants. An intriguing type of protective variant is buffering variants that protect against variants that have deleterious effects. We genotyped 18 variants in 15 genes related to longevity or healthy aging that had been previously reported as having a gene-gene interaction or buffering effect. We compared a group of 446 healthy oldest-old 'Super-Seniors' (individuals 85 or older who have never been diagnosed with cancer, cardiovascular disease, dementia, diabetes or major pulmonary disease) to 421 random population-based midlife controls. Cases and controls were of European ancestry. Association tests of individual SNPs showed that Super-Seniors were less likely than controls to carry an APOEε4 allele or a haptoglobin HP2 allele. Interactions between APOE/FOXO3, APOE/CRYL1, and LPA/CRYL1 did not remain significant after multiple testing correction. In a network analysis of the candidate genes, lipid and cholesterol metabolism was a common theme. APOE, HP, and CRYL1 have all been associated with Alzheimer's Disease, the pathology of which involves lipid and cholesterol pathways. Age-related changes in lipid and cholesterol maintenance, particularly in the brain, may be central to healthy aging and longevity.
MeSH term(s)	Aged, 80 and over ; Aging/genetics ; Alzheimer Disease/genetics ; Epistasis, Genetic ; Female ; Gene Regulatory Networks ; Genetic Predisposition to Disease/genetics ; Genotype ; Humans ; Lipid Metabolism/genetics ; Longevity/genetics ; Male ; Polymerase Chain Reaction ; Polymorphism, Single Nucleotide
Language	English
Publishing date	2017-03-28
Publishing country	United States
Document type	Journal Article
ZDB-ID	2560162-3
ISSN	1949-2553 ; 1949-2553
ISSN (online)	1949-2553
ISSN	1949-2553
DOI	10.18632/oncotarget.15296
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Article ; Online: Influenza Classification Suite: An automated Galaxy workflow for rapid influenza sequence analysis.

Eisler, Diane / Fornika, Dan / Tindale, Lauren C / Chan, Tracy / Sabaiduc, Suzana / Hickman, Rebecca / Chambers, Catharine / Krajden, Mel / Skowronski, Danuta M / Jassem, Agatha / Hsiao, William

Influenza and other respiratory viruses

2020 Volume 14, Issue 3, Page(s) 358–362

Abstract: Influenza viruses continually evolve to evade population immunity, and the different lineages are assigned into clades based on shared mutations. We have developed a publicly available computational workflow, the Influenza Classification Suite, for rapid ...

Abstract	Influenza viruses continually evolve to evade population immunity, and the different lineages are assigned into clades based on shared mutations. We have developed a publicly available computational workflow, the Influenza Classification Suite, for rapid clade mapping of sequenced influenza viruses. This suite provides a user-friendly workflow implemented in Galaxy to automate clade calling and antigenic site extraction. Workflow input includes clade definition and amino acid index array files, which can be customized to identify any clades of interest. The Influenza Classification Suite provides rapid, high-resolution understanding of circulating influenza strain evolution to inform influenza vaccine effectiveness and the need for potential vaccine reformulation.
MeSH term(s)	Classification/methods ; Humans ; Influenza, Human/virology ; Orthomyxoviridae/classification ; Orthomyxoviridae/genetics ; Orthomyxoviridae/isolation & purification ; Phylogeny ; Sequence Analysis, DNA ; Workflow
Language	English
Publishing date	2020-02-16
Publishing country	England
Document type	Evaluation Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2274538-5
ISSN	1750-2659 ; 1750-2640
ISSN (online)	1750-2659
ISSN	1750-2640
DOI	10.1111/irv.12722
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Article ; Online: Evidence for transmission of COVID-19 prior to symptom onset.

Tindale, Lauren C / Stockdale, Jessica E / Coombe, Michelle / Garlock, Emma S / Lau, Wing Yin Venus / Saraswat, Manu / Zhang, Louxin / Chen, Dongxuan / Wallinga, Jacco / Colijn, Caroline

eLife

2020 Volume 9

Abstract: We collated contact tracing data from COVID-19 clusters in Singapore and Tianjin, China and estimated the extent of pre-symptomatic transmission by estimating incubation periods and serial intervals. The mean incubation periods accounting for ... ...

Abstract	We collated contact tracing data from COVID-19 clusters in Singapore and Tianjin, China and estimated the extent of pre-symptomatic transmission by estimating incubation periods and serial intervals. The mean incubation periods accounting for intermediate cases were 4.91 days (95%CI 4.35, 5.69) and 7.54 (95%CI 6.76, 8.56) days for Singapore and Tianjin, respectively. The mean serial interval was 4.17 (95%CI 2.44, 5.89) and 4.31 (95%CI 2.91, 5.72) days (Singapore, Tianjin). The serial intervals are shorter than incubation periods, suggesting that pre-symptomatic transmission may occur in a large proportion of transmission events (0.4-0.5 in Singapore and 0.6-0.8 in Tianjin, in our analysis with intermediate cases, and more without intermediates). Given the evidence for pre-symptomatic transmission, it is vital that even individuals who appear healthy abide by public health measures to control COVID-19.
MeSH term(s)	Asymptomatic Diseases/epidemiology ; Betacoronavirus ; COVID-19 ; China/epidemiology ; Coronavirus Infections/epidemiology ; Coronavirus Infections/prevention & control ; Coronavirus Infections/transmission ; Humans ; Infectious Disease Incubation Period ; Pandemics/prevention & control ; Pneumonia, Viral/epidemiology ; Pneumonia, Viral/prevention & control ; Pneumonia, Viral/transmission ; SARS-CoV-2 ; Singapore/epidemiology ; Time Factors
Keywords	covid19
Language	English
Publishing date	2020-06-22
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2687154-3
ISSN	2050-084X ; 2050-084X
ISSN (online)	2050-084X
ISSN	2050-084X
DOI	10.7554/eLife.57149
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Article: Extraction and Detection of Avian Influenza Virus From Wetland Sediment Using Enrichment-Based Targeted Resequencing.

Tindale, Lauren C / Baticados, Waren / Duan, Jun / Coombe, Michelle / Jassem, Agatha / Tang, Patrick / Uyaguari-Diaz, Miguel / Moore, Richard / Himsworth, Chelsea / Hsiao, William / Prystajecky, Natalie

Frontiers in veterinary science

2020 Volume 7, Page(s) 301

Abstract: Early virus detection and characterization is key to successful avian influenza virus (AIV) surveillance for the health of humans as well as domestic poultry. We explored a novel sampling approach and molecular strategy using sediment from wetlands and ... ...

Abstract	Early virus detection and characterization is key to successful avian influenza virus (AIV) surveillance for the health of humans as well as domestic poultry. We explored a novel sampling approach and molecular strategy using sediment from wetlands and outdoor waterbodies on poultry farms as a population-level proxy of AIV activity in waterfowls. RNA was extracted using the MoBio RNA PowerSoil Total RNA isolation kit with additional chloroform extraction steps to reduce PCR inhibition. AIV matrix protein (MP) gene was detected in 42/345 (12.2%) samples by RT-qPCR; an additional 64 (18.6%) samples showed evidence of amplification below the threshold and were categorized as "suspect positive." Enrichment-based targeted resequencing (TR) identified AIV sequences in 79/345 (22.9%) samples. TR probes were designed for MP, hemagglutinin (HA), and neuraminidase (NA), however PB2 and PA were also identified. Although RT-qPCR and TR only had fair-moderate agreement, RT-qPCR positivity was predictive of TR-positivity both when using only strictly positive RT-qPCR samples (OR = 11.29) and when coding suspect positives as positive (OR = 7.56). This indicates that RT-qPCR could be used as a screening tool to select samples for virus characterization by TR and that future studies should consider RT-qPCR suspect positives to be positive samples for subsequent resequencing when avoiding false negatives is the priority, for instance in a diagnostic test, and to consider suspect positives to be negative samples when cost efficiency over a large number of samples is the priority, for instance in a surveillance program. A total of 13 HA (H1-7, H9-13, H16) and 9 NA (N1-9) subtypes were identified, with a maximum of 8 HA and 8 NA subtypes detected in a single sample. The optimized RNA extraction and targeted resequencing methods provided increased virus detection and subtyping characterization that could be implemented in an AIV surveillance system.
Language	English
Publishing date	2020-05-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2834243-4
ISSN	2297-1769
ISSN	2297-1769
DOI	10.3389/fvets.2020.00301
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