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Article ; Online: Detection of chicken chapparvovirus 2 in chickens with hemorrhagic hepatitis in Japan.

Fujino, Kan / Horie, Masayuki / Aihara, Naoyuki / Kamiie, Junichi / Taharaguchi, Satoshi

The Journal of veterinary medical science

2024 Volume 86, Issue 4, Page(s) 396–399

Abstract: Chicken chaphamaparvovirus causes diarrheal symptoms and can be detected in fecal samples. This study reports the detection of chicken chapparvovirus 2 in debilitated chickens with hemorrhagic hepatitis at a broiler farm in Japan. After euthanasia and ... ...

Abstract	Chicken chaphamaparvovirus causes diarrheal symptoms and can be detected in fecal samples. This study reports the detection of chicken chapparvovirus 2 in debilitated chickens with hemorrhagic hepatitis at a broiler farm in Japan. After euthanasia and necropsy, liver hemorrhage was observed. Nuclear inclusion bodies in the hepatocytes were identified using histological analysis. High-throughput sequencing analysis using RNA from livers of three affected chickens revealed infection by chicken chapparvovirus 2 and chicken anemia virus. Polymerase chain reaction analysis showed that all three chickens were positive for chicken chapparvovirus 2, and only one was positive for both chicken chapparvovirus 2 and chicken anemia virus. In conclusion, chicken chapparvovirus 2 causes infection in chickens in Japan and might be involved in hemorrhagic hepatitis.
MeSH term(s)	Animals ; Chickens ; Japan/epidemiology ; Hepatitis A/veterinary ; Hepatitis ; Hemorrhage/veterinary ; Poultry Diseases ; Chicken anemia virus
Language	English
Publishing date	2024-02-09
Publishing country	Japan
Document type	Journal Article
ZDB-ID	1071753-5
ISSN	1347-7439 ; 0916-7250
ISSN (online)	1347-7439
ISSN	0916-7250
DOI	10.1292/jvms.23-0415
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Article ; Online: Performing the hand laterality judgement task does not necessarily require motor imagery.

Mibu, Akira / Kan, Shigeyuki / Nishigami, Tomohiko / Fujino, Yuji / Shibata, Masahiko

Scientific reports

2020 Volume 10, Issue 1, Page(s) 5155

Abstract: When people judge the laterality of rotated hand images, that is they perform the laterality judgement task (LJT), they are thought to use motor imagery. However, recent studies have suggested that its completion does not necessarily require the use of ... ...

Abstract	When people judge the laterality of rotated hand images, that is they perform the laterality judgement task (LJT), they are thought to use motor imagery. However, recent studies have suggested that its completion does not necessarily require the use of motor imagery. In this study, we investigated whether and how many people preferentially use motor imagery to perform the LJT in 37 healthy adults. We assessed the presence of behavioural features associated with motor imagery at the individual level, namely, the linear angle-response time (RT) relationship and the biomechanical constraints effect in the LJT and in the same-different judgement task (SDJT), in which people are not thought to use motor imagery. We found that at most 50% of participants showed both behavioural features in the palmar view condition of the LJT. Moreover, this proportion did not differ from that in the dorsal view condition of the LJT or that in both view conditions of the SDJT. These results demonstrate that a motor imagery-based strategy is not universally and specifically used to perform the LJT. Therefore, previous results of the LJT, in particular, regarding the biomechanical constraints effect, should be reinterpreted in light of our findings.
MeSH term(s)	Adult ; Female ; Functional Laterality/physiology ; Hand/physiology ; Humans ; Imagery, Psychotherapy/methods ; Imagination/physiology ; Judgment/physiology ; Male ; Psychomotor Performance/physiology ; Reaction Time/physiology ; Rotation
Language	English
Publishing date	2020-03-20
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-61937-9
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Reverse genetics of parrot bornavirus 4 reveals a unique splicing of the glycoprotein gene that affects viral propagation.

Komorizono, Ryo / Fujino, Kan / Kessler, Susanne / Runge, Solveig / Kanda, Takehiro / Horie, Masayuki / Makino, Akiko / Rubbenstroth, Dennis / Tomonaga, Keizo

Journal of virology

2023 Volume 97, Issue 8, Page(s) e0050923

Abstract: Viruses can utilize host splicing machinery to enable the expression of multiple genes from a limited-sized genome. Orthobornaviruses use alternative splicing to regulate the expression level of viral proteins and achieve efficient viral replication in ... ...

Abstract	Viruses can utilize host splicing machinery to enable the expression of multiple genes from a limited-sized genome. Orthobornaviruses use alternative splicing to regulate the expression level of viral proteins and achieve efficient viral replication in the nucleus. Although more than 20 orthobornaviruses have been identified belonging to eight different viral species, virus-specific splicing has not been demonstrated. Here, we demonstrate that the glycoprotein (G) transcript of parrot bornavirus 4 (PaBV-4; species
MeSH term(s)	Animals ; Bird Diseases ; Bornaviridae/genetics ; Glycoproteins/genetics ; Mononegavirales Infections/pathology ; Mononegavirales Infections/virology ; Parrots/genetics ; Protein Isoforms/genetics ; Reverse Genetics ; RNA, Messenger
Chemical Substances	Glycoproteins ; Protein Isoforms ; RNA, Messenger
Language	English
Publishing date	2023-08-14
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80174-4
ISSN	1098-5514 ; 0022-538X
ISSN (online)	1098-5514
ISSN	0022-538X
DOI	10.1128/jvi.00509-23
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Article ; Online: Potential of the EVAHEART 2 Double-Cuff Tipless Inflow Cannula for Prevention of Thromboembolic Events.

Ushijima, Tomoki / Tanoue, Yoshihisa / Sonoda, Hiromichi / Kan-O, Meikun / Oda, Shinichiro / Kimura, Satoshi / Hashimoto, Toru / Fujino, Takeo / Shiose, Akira

ASAIO journal (American Society for Artificial Internal Organs : 1992)

2022 Volume 68, Issue 10, Page(s) e168–e171

Abstract: A 32-year-old man, who had developed fulminant myocarditis leading to asystole, underwent implantation of an EVAHEART 2 left ventricular assist system with a double-cuff tipless inflow cannula and a concurrent Fontan operation. Approximately 2 years ... ...

Abstract	A 32-year-old man, who had developed fulminant myocarditis leading to asystole, underwent implantation of an EVAHEART 2 left ventricular assist system with a double-cuff tipless inflow cannula and a concurrent Fontan operation. Approximately 2 years after the simultaneous EVAHEART 2 implantation and the Fontan operation, the patient underwent heart transplantation. There was no device-related thromboembolism or pump malfunction under adequate antithrombotic management during the postoperative support period. Computed tomography showed no malposition of the inflow cannula irrespective of the left ventricular chamber size. Macroscopically, the left ventricular cavity of the excised heart revealed a smooth inflow ostium with appropriate intimal proliferation and without pannus or wedge thrombus formation. These findings suggest the utility of the double-cuff tipless inflow cannula for long-term clinical applications, which may lead to favorable outcomes during long-term patient management. The double-cuff tipless inflow cannula, which does not protrude into the left ventricular cavity, potentially contributes to the prevention of suction events and the collision of the inflow cannula with the interventricular septum and left ventricular free wall. Further investigation is required to confirm the role of the unique EVAHEART 2 inflow cannula in reducing thromboembolic events.
MeSH term(s)	Adult ; Cannula ; Fibrinolytic Agents ; Heart Ventricles/surgery ; Heart-Assist Devices/adverse effects ; Humans ; Male ; Thromboembolism/etiology ; Thromboembolism/prevention & control
Chemical Substances	Fibrinolytic Agents
Language	English
Publishing date	2022-03-01
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	759982-1
ISSN	1538-943X ; 0162-1432 ; 1058-2916
ISSN (online)	1538-943X
ISSN	0162-1432 ; 1058-2916
DOI	10.1097/MAT.0000000000001672
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Uk I Zs.199: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.B 2435: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Verbal Instruction Can Induce Extinction of Fear of Movement-Related Pain.

Maeda, Yoshiki / Kan, Shigeyuki / Fujino, Yuji / Shibata, Masahiko

The journal of pain : official journal of the American Pain Society

2018 Volume 19, Issue 9, Page(s) 1063–1073

Abstract: The fear avoidance model of chronic musculoskeletal pain highlights the importance of pain-related fear in chronification of pain. Although several interventions have been developed on the basis of this model, the following issues remain unresolved: ... ...

Abstract	The fear avoidance model of chronic musculoskeletal pain highlights the importance of pain-related fear in chronification of pain. Although several interventions have been developed on the basis of this model, the following issues remain unresolved: first, whether movement conditioned to pain can evoke fear responses particularly sympathetic activation, and second, whether verbal instructions can attenuate conditioned fear of movement-related pain as with direct experience. To investigate these issues, we induced proprioceptive conditioning (learning the relationship between proprioceptive sensations and an aversive event) and extinction learning in healthy volunteers, and we compared psychophysiological and subjective indices of fear between an instructed and a normal extinction group. Using paired presentation of painful heat stimuli as an unconditioned stimulus and flexion of the wrist as a conditioned stimulus, all participants acquired the conditioned fear response (skin potential response) to the conditioned stimulus. The instructed extinction group was then told that the movement was no longer followed by painful stimulus at the beginning of the extinction phase, and only this group showed significant decreases on both indices of fear. This finding indicates that verbal instruction can attenuate conditioned fear of movement-related pain, supporting the clinical importance of providing information regarding the relationship between movement and pain. Perspective: This article presents evidence that, in a laboratory setting, verbal instructions rapidly decrease conditioned fear of movement-related pain on psychophysiological as well as subjective indices. This result indicates that it is important for chronic musculoskeletal pain patients to precisely understand the relationship between movement and pain in clinical situations.
MeSH term(s)	Adult ; Avoidance Learning/physiology ; Extinction, Psychological/physiology ; Fear/physiology ; Fear/psychology ; Female ; Humans ; Male ; Movement/physiology ; Pain/physiopathology ; Pain/psychology ; Young Adult
Language	English
Publishing date	2018-04-24
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2018789-0
ISSN	1528-8447 ; 1526-5900
ISSN (online)	1528-8447
ISSN	1526-5900
DOI	10.1016/j.jpain.2018.03.014
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Zs.A 5237: Show issues

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Article ; Online: Coronary artery calcium among patients with heterozygous familial hypercholesterolaemia.

Tada, Hayato / Kojima, Nobuko / Yamagami, Kan / Nomura, Akihiro / Nohara, Atsushi / Usui, Soichiro / Sakata, Kenji / Hayashi, Kenshi / Fujino, Noboru / Takamura, Masayuki / Kawashiri, Masa-Aki

European heart journal open

2023 Volume 3, Issue 3, Page(s) oead046

Abstract: Aims: We aimed to determine if coronary artery calcium (CAC) is associated with cardiovascular disease (CVD) events, defined as CVD-related death, unstable angina, myocardial infarction, or staged revascularization among patients with heterozygous ... ...

Abstract	Aims: We aimed to determine if coronary artery calcium (CAC) is associated with cardiovascular disease (CVD) events, defined as CVD-related death, unstable angina, myocardial infarction, or staged revascularization among patients with heterozygous familial hypercholesterolaemia (HeFH) under primary prevention settings. Methods and results: Data of patients with FH admitted to Kanazawa University Hospital between 2000 and 2020, who underwent CAC measurement and were followed up ( Conclusion: The CAC score helps in further risk stratification in patients with HeFH.
Language	English
Publishing date	2023-05-03
Publishing country	England
Document type	Journal Article
ISSN	2752-4191
ISSN (online)	2752-4191
DOI	10.1093/ehjopen/oead046
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Effects of Different Types of Pathogenic Variants on Phenotypes of Familial Hypercholesterolemia.

Tada, Hayato / Kojima, Nobuko / Yamagami, Kan / Nomura, Akihiro / Nohara, Atsushi / Usui, Soichiro / Sakata, Kenji / Fujino, Noboru / Takamura, Masayuki / Kawashiri, Masa-Aki

Frontiers in genetics

2022 Volume 13, Page(s) 872056

Abstract: Objective: ...

Abstract	Objective:
Language	English
Publishing date	2022-04-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606823-0
ISSN	1664-8021
ISSN	1664-8021
DOI	10.3389/fgene.2022.872056
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Article ; Online: Impact of Healthy Lifestyle in Patients With Familial Hypercholesterolemia.

Tada, Hayato / Kojima, Nobuko / Yamagami, Kan / Nomura, Akihiro / Nohara, Atsushi / Usui, Soichiro / Sakata, Kenji / Hayashi, Kenshi / Fujino, Noboru / Takamura, Masayuki / Kawashiri, Masa-Aki

JACC. Asia

2023 Volume 3, Issue 1, Page(s) 152–160

Abstract: Background: Pathogenic mutations are associated with poor outcomes in patients with familial hypercholesterolemia (FH). However, data on the effects of a healthy lifestyle on FH phenotypes are limited.: Objectives: The authors investigated the ... ...

Abstract	Background: Pathogenic mutations are associated with poor outcomes in patients with familial hypercholesterolemia (FH). However, data on the effects of a healthy lifestyle on FH phenotypes are limited. Objectives: The authors investigated the interaction between a healthy lifestyle and FH mutation with prognosis in patients with FH. Methods: We investigated the associations of the interaction between genotypes and lifestyle, with the occurrence of major adverse cardiac events (MACE), such as cardiovascular-related mortality, myocardial infarction, unstable angina, and coronary artery revascularization, in patients with FH. We assessed their lifestyle based on 4 questionnaires (healthy dietary pattern, regular exercise, not smoking, and absence of obesity). The Cox proportional hazards model was used to assess the risk for MACE. Results: The median follow-up duration was 12.6 (IQR: 9.5-17.9) years. During the follow-up duration, 179 MACE were observed. Independent of classic risk factors, FH mutation and lifestyle score were significantly associated with MACE (HR: 2.73; 95% CI: 1.03-4.43; Conclusions: A healthy lifestyle was associated with reduced risk for MACE among patients with FH with or without genetic diagnosis.
Language	English
Publishing date	2023-01-31
Publishing country	United States
Document type	Journal Article
ISSN	2772-3747
ISSN (online)	2772-3747
DOI	10.1016/j.jacasi.2022.10.012
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Reverse genetics of parrot bornavirus 4 reveals a unique splicing of the glycoprotein gene that affects viral propagation

Komorizono, Ryo / Fujino, Kan / Kessler, Susanne / Runge, Solveig / Kanda, Takehiro / Horie, Masayuki / Makino, Akiko / Rubbenstroth, Dennis / Tomonaga, Keizo

2023

Abstract	Viruses can utilize host splicing machinery to enable the expression of multiple genes from a limited-sized genome. Orthobornaviruses use alternative splicing to regulate the expression level of viral proteins and achieve efficient viral replication in the nucleus. Although more than 20 orthobornaviruses have been identified belonging to eight different viral species, virus-specific splicing has not been demonstrated. Here, we demonstrate that the glycoprotein (G) transcript of parrot bornavirus 4 (PaBV-4; species Orthobornavirus alphapsittaciforme), a highly virulent virus in psittacines, undergoes mRNA splicing and expresses a soluble isoform termed sGP. Interestingly, the splicing donor for sGP is not conserved in other orthobornaviruses, including those belonging to the same orthobornavirus species, suggesting that this splicing has evolved as a PaBV-4-specific event. We have also shown that exogenous expression of sGP does not affect PaBV-4 replication or de novo virion infectivity. In this study, to investigate the role of sGP in viral replication, we established a reverse genetics system for PaBV-4 by using avian cell lines and generated a recombinant virus lacking the spliced mRNA for sGP. Using the recombinant viruses, we show that the replication of the sGP-deficient virus is significantly slower than that of the wild-type virus and that the exogenous expression of sGP cannot restore its propagation efficiency. These results suggest that autologous or controlled expression of sGP by splicing may be important for PaBV-4 propagation. The reverse genetics system for avian bornaviruses developed here will be a powerful tool for understanding the replication strategies and pathogenesis of avian orthobornaviruses.
Keywords	Text ; ddc:570 ; bornavirus -- avian virus -- proventricular dilatation disease -- RNA splicing -- reverse genetic analysis
Subject code	570 ; 339
Language	English
Publishing date	2023-08-31
Publisher	American Society for Microbiology
Publishing country	de
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Cellular and humoral immune responses to COVID-19 booster vaccination in Japanese dialysis patients.

Clinical and experimental nephrology

2024

Abstract: Background: Dialysis patients are susceptible to developing severe coronavirus disease 2019 (COVID-19) due to hypoimmunity. Antibody titers against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) after the primary vaccinations are lower in ... ...

Abstract	Background: Dialysis patients are susceptible to developing severe coronavirus disease 2019 (COVID-19) due to hypoimmunity. Antibody titers against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) after the primary vaccinations are lower in hemodialysis (HD) patients than in healthy individuals. This study aimed to evaluate the effect of a SARS-CoV-2 booster vaccination in HD and peritoneal dialysis (PD) patients based on antibody titers and cellular and humoral immunity. Methods: Participants of the control, HD, and PD groups were recruited from 12 facilities. SARS-CoV-2 antigen-specific cytokine and IgG-antibody levels were measured. Regulatory T cells and memory B cells were counted using flow cytometry at 6 months after primary vaccination with BNT162b2 and 3 weeks after the booster vaccination in HD and PD patients and compared with those of a control group. Results: Booster vaccination significantly enhanced the levels of antibodies, cytokines, and memory B cells in three groups. The HD group showed significantly higher levels of IgG-antibodies, IL-1β, IL-2, IL-4, IL-17, and memory B cells than those in the control group at 3 weeks after the booster dose. The PD group tended to show similar trends to HD patients but had similar levels of IgG-antibodies, cytokines, and memory B cells to the control group. Conclusions: HD patients had significantly stronger cellular and humoral immune responses than the control 3 weeks after the booster dose. Our findings will help in developing better COVID-19 vaccination strategies for HD and PD patients.
Language	English
Publishing date	2024-03-08
Publishing country	Japan
Document type	Journal Article
ZDB-ID	1338768-6
ISSN	1437-7799 ; 1342-1751
ISSN (online)	1437-7799
ISSN	1342-1751
DOI	10.1007/s10157-024-02477-8
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