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Article ; Online: Live-attenuated auxotrophic mutant of Salmonella Typhimurium expressing immunogenic HA1 protein enhances immunity and protective efficacy against H1N1 influenza virus infection.

Kamble, Nitin Machindra / Hyoung, Kim Je / Lee, John Hwa

Future microbiology

2017 Volume 12, Page(s) 739–752

Abstract: Aim: To evaluate the efficacy of attenuated Salmonella Typhimurium (JOL912) as a live bacterial vaccine vector.: Materials & methods: The JOL912 engineered to deliver HA1 protein from influenza A/Puerto Rico/8/1934 (H1N1; PR8) virus was coined as ... ...

Abstract	Aim: To evaluate the efficacy of attenuated Salmonella Typhimurium (JOL912) as a live bacterial vaccine vector. Materials & methods: The JOL912 engineered to deliver HA1 protein from influenza A/Puerto Rico/8/1934 (H1N1; PR8) virus was coined as JOL1635 and further evaluated for immunogenicity and protective efficacy. Results: The JOL1635 stably harbored the HA1 gene within pMMP65 plasmid with periplasmic expression and effective delivery of HA1 protein to RAW264.7 cells. The JOL1635 immunized chickens showed the significant increase in HA1-specific IgG, sIgA antibody, IFN-γ, IL-6 cytokine and cellular immune responses. The postoral challenge, the JOL1635-immunized chickens showed a faster clearance of PR8 virus cloacal shedding than the control group. Conclusion: Generated JOL1635 can establish specific immunogenicity and protection against the PR8 virus in chickens.
Language	English
Publishing date	2017-07
Publishing country	England
Document type	Journal Article
ISSN	1746-0921
ISSN (online)	1746-0921
DOI	10.2217/fmb-2016-0190
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Article ; Online: Orally administered live attenuated Salmonella Typhimurium protects mice against lethal infection with H1N1 influenza virus.

Kamble, Nitin Machindra / Hajam, Irshad Ahmed / Lee, John Hwa

Veterinary microbiology

2017 Volume 201, Page(s) 1–6

Abstract: Pre-stimulation of toll-like receptors (TLRs) by agonists has been shown to increase protection against influenza virus infection. In this study, we evaluated the protective response generated against influenza A/Puerto Rico/8/1934 (PR8; H1N1) virus by ... ...

Abstract	Pre-stimulation of toll-like receptors (TLRs) by agonists has been shown to increase protection against influenza virus infection. In this study, we evaluated the protective response generated against influenza A/Puerto Rico/8/1934 (PR8; H1N1) virus by oral and nasal administration of live attenuated Salmonella enterica serovar Typhimurium, JOL911 strain, in mice. Oral and nasal inoculation of JOL911 significantly increased the mRNA copy number of TLR-2, TLR4 and TLR5, and downstream type I interferon (IFN) molecules, IFN-α and IFN-β, both in peripheral blood mononuclear cells (PBMCs) and in lung tissue. Similarly, the mRNA copy number of interferon-inducible genes (ISGs), Mx and ISG15, were significantly increased in both the orally and the nasally inoculated mice. Post PR8 virus lethal challenge, the nasal JOL911 and the PBS control group mice showed significant loss of body weight with 70% and 100% mortality, respectively, compared to only 30% mortality in the oral JOL911 group mice. Post sub-lethal challenge, the significant reduction in PR8 virus copy number in lung tissue was observed in oral [on day 4 and 6 post-challenge (dpc)] and nasal (on 4dpc) than the PBS control group mice. The lethal and sub-lethal challenge showed that the generated stimulated innate resistance (StIR) in JOL911 inoculated mice conferred resistance to acute and initial influenza infection but might not be sufficient to prevent the PR8 virus invasion and replication in the lung. Overall, the present study indicates that oral administration of attenuated S. Typhimurium can pre-stimulate multiple TLR pathways in mice to provide immediate early StIR against a lethal H1N1 virus challenge.
MeSH term(s)	Administration, Intranasal ; Administration, Oral ; Animals ; Humans ; Immunity, Innate ; Influenza A Virus, H1N1 Subtype/immunology ; Influenza Vaccines/administration & dosage ; Influenza, Human/pathology ; Influenza, Human/prevention & control ; Influenza, Human/virology ; Interferons/immunology ; Leukocytes, Mononuclear/immunology ; Lung/pathology ; Lung/virology ; Mice ; Mice, Inbred BALB C ; Orthomyxoviridae Infections/pathology ; Orthomyxoviridae Infections/prevention & control ; Orthomyxoviridae Infections/veterinary ; Orthomyxoviridae Infections/virology ; Salmonella typhimurium/immunology ; Vaccination ; Vaccines, Attenuated/administration & dosage
Chemical Substances	Influenza Vaccines ; Vaccines, Attenuated ; Interferons (9008-11-1)
Language	English
Publishing date	2017-03
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	753154-0
ISSN	1873-2542 ; 0378-1135
ISSN (online)	1873-2542
ISSN	0378-1135
DOI	10.1016/j.vetmic.2017.01.006
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Article ; Online: Intracellular delivery of HA1 subunit antigen through attenuated Salmonella Gallinarum act as a bivalent vaccine against fowl typhoid and low pathogenic H5N3 virus.

Kamble, Nitin Machindra / Hyoung, Kim Je / Lee, John Hwa

Veterinary research

2017 Volume 48, Issue 1, Page(s) 40

Abstract: Introduction of novel inactivated oil-emulsion vaccines against different strains of prevailing and emerging low pathogenic avian influenza (LPAI) viruses is not an economically viable option for poultry. Engineering attenuated Salmonella Gallinarum (S. ... ...

Abstract	Introduction of novel inactivated oil-emulsion vaccines against different strains of prevailing and emerging low pathogenic avian influenza (LPAI) viruses is not an economically viable option for poultry. Engineering attenuated Salmonella Gallinarum (S. Gallinarum) vaccine delivering H5 LPAI antigens can be employed as a bivalent vaccine against fowl typhoid and LPAI viruses, while still offering economic viability and sero-surveillance capacity. In this study, we developed a JOL1814 bivalent vaccine candidate against LPAI virus infection and fowl typhoid by engineering the attenuated S. Gallinarum to deliver the globular head (HA1) domain of hemagglutinin protein from H5 LPAI virus through pMMP65 constitutive expression plasmid. The important feature of the developed JOL1814 was the delivery of the HA1 antigen to cytosol of peritoneal macrophages. Immunization of chickens with JOL1814 produced significant level of humoral, mucosal, cellular and IL-2, IL-4, IL-17 and IFN-γ cytokine immune response against H5 HA1 and S. Gallinarum antigens in the immunized chickens. Post-challenge, only the JOL1814 immunized chicken showed significantly faster clearance of H5N3 virus in oropharyngeal and cloacal swabs, and 90% survival rate against lethal challenge with a wild type S. Gallinarum. Furthermore, the JOL1814 immunized were differentiated from the H5N3 LPAI virus infected chickens by matrix (M2) gene-specific real-time PCR. In conclusion, the data from the present showed that the JOL1814 can be an effective bivalent vaccine candidate against H5N3 LPAI and fowl typhoid infection in poultry while still offering sero-surveillance property against H5 avian influenza virus.
MeSH term(s)	Animals ; Antigens, Viral/administration & dosage ; Antigens, Viral/immunology ; Chickens/immunology ; Chickens/microbiology ; Chickens/virology ; Genetic Engineering/methods ; Genetic Engineering/veterinary ; Influenza A Virus, H5N8 Subtype/immunology ; Influenza Vaccines/immunology ; Influenza Vaccines/therapeutic use ; Influenza in Birds/immunology ; Influenza in Birds/prevention & control ; Influenza in Birds/virology ; Poultry Diseases/immunology ; Poultry Diseases/microbiology ; Poultry Diseases/prevention & control ; Poultry Diseases/virology ; Salmonella/immunology ; Salmonella Infections, Animal/immunology ; Salmonella Infections, Animal/microbiology ; Salmonella Infections, Animal/prevention & control ; Vaccines, Attenuated/immunology ; Vaccines, Attenuated/therapeutic use ; Vaccines, Synthetic/immunology ; Vaccines, Synthetic/therapeutic use
Chemical Substances	Antigens, Viral ; Influenza Vaccines ; Vaccines, Attenuated ; Vaccines, Synthetic
Language	English
Publishing date	2017-08-07
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1146298-x
ISSN	1297-9716 ; 0928-4249
ISSN (online)	1297-9716
ISSN	0928-4249
DOI	10.1186/s13567-017-0446-1
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Article: Self-destructing Salmonella via temperature induced gene E of phage PhiX174 improves influenza HA DNA vaccine immune protection against H1N1 infection in mice model

Kamble, Nitin Machindra / Senevirathne, Amal / Koh, Hong Bum / Lee, Jae Il / Lee, John Hwa

Journal of immunological methods. 2019 Sept., v. 472

2019

Abstract: The delivery of DNA vaccines is the principle impediment for implementation of DNA vaccination on a mass scale. In this study, we report a temperature induced conditionally expressed phage PhiX174 gene E mediated lysis of Salmonella under in vivo ... ...

Abstract	The delivery of DNA vaccines is the principle impediment for implementation of DNA vaccination on a mass scale. In this study, we report a temperature induced conditionally expressed phage PhiX174 gene E mediated lysis of Salmonella under in vivo conditions that can increase the immunogenicity of a DNA vaccine delivered via Salmonella carrier system. We electroporated gene E encoding lysis plasmid pJHL187 along with the pcDNA-HA plasmid encoding H1N1 HA into attenuated Salmonella Typhimurium, strain JOL1893. Using C57BL/6 mice as the model, we showed that the mice intragastrically vaccinated with JOL1893 induced significant production of HA-specific humoral and cell mediated immune responses compared to the JOL1837, which carry pcDNA-HA plasmid alone. Furthermore, mice vaccinated with JOL1893 vaccine were fully protected against the lethal H1N1 challenge compared to the JOL1837 strain, which showed 90% protection only. However, none of the animals survived treated with either the PBS or the Salmonella carrying empty vector. Taken together, our results indicate that mucosal immunization with conditional lysis enabled live attenuated S. Typhimurium as a DNA vaccine carrier can induce efficient systemic and mucosal immune responses, and improves immune protection against a highly pathogenic H1N1 infection in mice model.
Keywords	Salmonella Typhimurium ; animal models ; bacteriophages ; genes ; immune response ; immunogenicity ; influenza ; live vaccines ; mice ; mucosal immunity ; plasmids ; recombinant vaccines ; temperature ; vaccination
Language	English
Dates of publication	2019-09
Size	p. 7-15.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	120142-6
ISSN	1872-7905 ; 0022-1759
ISSN (online)	1872-7905
ISSN	0022-1759
DOI	10.1016/j.jim.2019.06.008
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Self-destructing Salmonella via temperature induced gene E of phage PhiX174 improves influenza HA DNA vaccine immune protection against H1N1 infection in mice model.

Kamble, Nitin Machindra / Senevirathne, Amal / Koh, Hong Bum / Lee, Jae Il / Lee, John Hwa

Journal of immunological methods

2019 Volume 472, Page(s) 7–15

Abstract	The delivery of DNA vaccines is the principle impediment for implementation of DNA vaccination on a mass scale. In this study, we report a temperature induced conditionally expressed phage PhiX174 gene E mediated lysis of Salmonella under in vivo conditions that can increase the immunogenicity of a DNA vaccine delivered via Salmonella carrier system. We electroporated gene E encoding lysis plasmid pJHL187 along with the pcDNA-HA plasmid encoding H1N1 HA into attenuated Salmonella Typhimurium, strain JOL1893. Using C57BL/6 mice as the model, we showed that the mice intragastrically vaccinated with JOL1893 induced significant production of HA-specific humoral and cell mediated immune responses compared to the JOL1837, which carry pcDNA-HA plasmid alone. Furthermore, mice vaccinated with JOL1893 vaccine were fully protected against the lethal H1N1 challenge compared to the JOL1837 strain, which showed 90% protection only. However, none of the animals survived treated with either the PBS or the Salmonella carrying empty vector. Taken together, our results indicate that mucosal immunization with conditional lysis enabled live attenuated S. Typhimurium as a DNA vaccine carrier can induce efficient systemic and mucosal immune responses, and improves immune protection against a highly pathogenic H1N1 infection in mice model.
MeSH term(s)	Animals ; Bacteriolysis ; Bacteriophages/genetics ; Influenza A Virus, H1N1 Subtype/immunology ; Influenza Vaccines/immunology ; Interferon-gamma/genetics ; Mice ; Mice, Inbred C57BL ; Salmonella typhimurium/genetics ; Salmonella typhimurium/immunology ; Temperature ; Vaccination ; Vaccines, DNA/immunology
Chemical Substances	Influenza Vaccines ; Vaccines, DNA ; Interferon-gamma (82115-62-6)
Language	English
Publishing date	2019-06-06
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	120142-6
ISSN	1872-7905 ; 0022-1759
ISSN (online)	1872-7905
ISSN	0022-1759
DOI	10.1016/j.jim.2019.06.008
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Article ; Online: Interaction of a live attenuated Salmonella Gallinarum vaccine candidate with chicken bone marrow-derived dendritic cells.

Kamble, Nitin Machindra / Jawale, Chetan Vilas / Lee, John Hwa

Avian pathology : journal of the W.V.P.A

2016 Volume 45, Issue 2, Page(s) 235–243

Abstract: Salmonella enterica serovar Gallinarum (SG) is a Gram-negative intracellular host-adapted pathogen that causes fowl typhoid. Attenuated strains of SG are proven and widely used vaccine candidates because of advantages like induction of strong humoral and ...

Abstract	Salmonella enterica serovar Gallinarum (SG) is a Gram-negative intracellular host-adapted pathogen that causes fowl typhoid. Attenuated strains of SG are proven and widely used vaccine candidates because of advantages like induction of strong humoral and cell-mediated immune responses. In the present study, we investigated the interaction of chicken bone marrow-derived dendritic cells (chBM-DCs) with an attenuated SG (JOL1355) strain that secretes a heat-labile enterotoxin B subunit protein previously shown to successfully vaccinate chickens. ChBM-DCs were isolated and cultured in the presence of recombinant chicken GM-CSF and IL-4 cytokines. The chBM-DCs were infected with JOL1355 at an multiplicity of infection of 10. JOL1355 was able to invade dendritic cells (DCs); however, the survival of JOL1355 in DCs decreased over time. At 24 h post infection, IL-6, IL-10 and IFN-γ transcript levels were significantly increased in JOL1355-infected DCs compared to non-stimulated DCs. Flow cytometry analysis showed an increased proportion of cells producing CD40, CD80, and MHC class II in the JOL1355-infected cultures compared to the non-stimulated control. In addition, JOL1355-stimulated chBM-DCs could induce significant expression of IL-2 in co-culture with autologous CD4+ T cells. Based on these results, we conclude that chBM-DCs are capable of internalizing the live attenuated SG vaccine candidate and the infected chBM-DCs show signs of maturation as evidenced by the upregulated expression of costimulatory molecules and cytokines.
MeSH term(s)	Animals ; Bacterial Vaccines/immunology ; Bone Marrow/immunology ; Chickens/immunology ; Cytokines/immunology ; Dendritic Cells/immunology ; Enterotoxins/immunology ; Immunity, Cellular ; Poultry Diseases/prevention & control ; Salmonella Infections, Animal/prevention & control ; Salmonella enterica/immunology ; Vaccines, Attenuated
Chemical Substances	Bacterial Vaccines ; Cytokines ; Enterotoxins ; Vaccines, Attenuated
Language	English
Publishing date	2016
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1476380-1
ISSN	1465-3338 ; 0307-9457
ISSN (online)	1465-3338
ISSN	0307-9457
DOI	10.1080/03079457.2016.1144919
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Article ; Online: A live attenuated Salmonella Enteritidis secreting detoxified heat labile toxin enhances mucosal immunity and confers protection against wild-type challenge in chickens.

Lalsiamthara, Jonathan / Kamble, Nitin Machindra / Lee, John Hwa

Veterinary research

2016 Volume 47, Issue 1, Page(s) 60

Abstract: A live attenuated Salmonella Enteritidis (SE) capable of constitutively secreting detoxified double mutant Escherichia coli heat labile toxin (dmLT) was developed. The biologically adjuvanted strain was generated via transformation of a highly ... ...

Abstract	A live attenuated Salmonella Enteritidis (SE) capable of constitutively secreting detoxified double mutant Escherichia coli heat labile toxin (dmLT) was developed. The biologically adjuvanted strain was generated via transformation of a highly immunogenic SE JOL1087 with a plasmid encoding dmLT gene cassette; the resultant strain was designated JOL1641. A balanced-lethal host-vector system stably maintained the plasmid via auxotrophic host complementation with a plasmid encoded aspartate semialdehyde dehydrogenase (asd) gene. Characterization by western blot assay revealed the dmLT subunit proteins in culture supernatants of JOL1641. For the investigation of adjuvanticity and protective efficacy, chickens were immunized via oral or intramuscular routes with PBS, JOL1087 and JOL1641. Birds immunized with JOL1641 showed significant (P ≤ 0.05) increases in intestinal SIgA production at the 1(st) and 2(nd) weeks post-immunization via oral and intramuscular routes, respectively. Interestingly, while both strains showed significant splenic protection via intramuscular immunization, JOL1641 outperformed JOL1087 upon oral immunization. Oral immunization of birds with JOL1641 significantly reduced splenic bacterial counts. The reduction in bacterial counts may be correlated with an adjuvant effect of dmLT that increases SIgA secretion in the intestines of immunized birds. The inclusion of detoxified dmLT in the strain did not cause adverse reactions to birds, nor did it extend the period of bacterial fecal shedding. In conclusion, we report here that dmLT could be biologically incorporated in the secretion system of a live attenuated Salmonella-based vaccine, and that this construction is safe and could enhance mucosal immunity, and protect immunized birds against wild-type challenge.
MeSH term(s)	Animals ; Bacterial Toxins/immunology ; Chickens/immunology ; Chickens/microbiology ; Enterotoxins/immunology ; Escherichia coli Proteins/immunology ; Immunity, Mucosal/immunology ; Male ; Poultry Diseases/immunology ; Poultry Diseases/microbiology ; Poultry Diseases/prevention & control ; Salmonella Infections, Animal/immunology ; Salmonella Infections, Animal/microbiology ; Salmonella Infections, Animal/prevention & control ; Salmonella Vaccines/immunology ; Salmonella Vaccines/therapeutic use ; Salmonella enteritidis/immunology ; Vaccines, Attenuated/immunology ; Vaccines, Attenuated/therapeutic use
Chemical Substances	Bacterial Toxins ; Enterotoxins ; Escherichia coli Proteins ; Salmonella Vaccines ; Vaccines, Attenuated ; heat-labile enterotoxin, E coli (D9K3SN2LNY)
Language	English
Publishing date	2016-06-04
Publishing country	England
Document type	Journal Article
ZDB-ID	1146298-x
ISSN	1297-9716 ; 0928-4249
ISSN (online)	1297-9716
ISSN	0928-4249
DOI	10.1186/s13567-016-0348-7
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Article ; Online: Intracellular delivery of HA1 subunit antigen through attenuated Salmonella Gallinarum act as a bivalent vaccine against fowl typhoid and low pathogenic H5N3 virus

Nitin Machindra Kamble / Kim Je Hyoung / John Hwa Lee

Veterinary Research, Vol 48, Iss 1, Pp 1-

2017 Volume 12

Abstract: Abstract Introduction of novel inactivated oil-emulsion vaccines against different strains of prevailing and emerging low pathogenic avian influenza (LPAI) viruses is not an economically viable option for poultry. Engineering attenuated Salmonella ... ...

Abstract	Abstract Introduction of novel inactivated oil-emulsion vaccines against different strains of prevailing and emerging low pathogenic avian influenza (LPAI) viruses is not an economically viable option for poultry. Engineering attenuated Salmonella Gallinarum (S. Gallinarum) vaccine delivering H5 LPAI antigens can be employed as a bivalent vaccine against fowl typhoid and LPAI viruses, while still offering economic viability and sero-surveillance capacity. In this study, we developed a JOL1814 bivalent vaccine candidate against LPAI virus infection and fowl typhoid by engineering the attenuated S. Gallinarum to deliver the globular head (HA1) domain of hemagglutinin protein from H5 LPAI virus through pMMP65 constitutive expression plasmid. The important feature of the developed JOL1814 was the delivery of the HA1 antigen to cytosol of peritoneal macrophages. Immunization of chickens with JOL1814 produced significant level of humoral, mucosal, cellular and IL-2, IL-4, IL-17 and IFN-γ cytokine immune response against H5 HA1 and S. Gallinarum antigens in the immunized chickens. Post-challenge, only the JOL1814 immunized chicken showed significantly faster clearance of H5N3 virus in oropharyngeal and cloacal swabs, and 90% survival rate against lethal challenge with a wild type S. Gallinarum. Furthermore, the JOL1814 immunized were differentiated from the H5N3 LPAI virus infected chickens by matrix (M2) gene-specific real-time PCR. In conclusion, the data from the present showed that the JOL1814 can be an effective bivalent vaccine candidate against H5N3 LPAI and fowl typhoid infection in poultry while still offering sero-surveillance property against H5 avian influenza virus.
Keywords	Veterinary medicine ; SF600-1100
Subject code	616
Language	English
Publishing date	2017-08-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
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Article: Orally administered live attenuated Salmonella Typhimurium protects mice against lethal infection with H1N1 influenza virus

Kamble, Nitin Machindra / Irshad Ahmed Hajam / John Hwa Lee

Veterinary microbiology. 2017 Mar., v. 201

2017

Abstract	Pre-stimulation of toll-like receptors (TLRs) by agonists has been shown to increase protection against influenza virus infection. In this study, we evaluated the protective response generated against influenza A/Puerto Rico/8/1934 (PR8; H1N1) virus by oral and nasal administration of live attenuated Salmonella enterica serovar Typhimurium, JOL911 strain, in mice. Oral and nasal inoculation of JOL911 significantly increased the mRNA copy number of TLR-2, TLR4 and TLR5, and downstream type I interferon (IFN) molecules, IFN-α and IFN-β, both in peripheral blood mononuclear cells (PBMCs) and in lung tissue. Similarly, the mRNA copy number of interferon-inducible genes (ISGs), Mx and ISG15, were significantly increased in both the orally and the nasally inoculated mice. Post PR8 virus lethal challenge, the nasal JOL911 and the PBS control group mice showed significant loss of body weight with 70% and 100% mortality, respectively, compared to only 30% mortality in the oral JOL911 group mice. Post sub-lethal challenge, the significant reduction in PR8 virus copy number in lung tissue was observed in oral [on day 4 and 6 post-challenge (dpc)] and nasal (on 4dpc) than the PBS control group mice. The lethal and sub-lethal challenge showed that the generated stimulated innate resistance (StIR) in JOL911 inoculated mice conferred resistance to acute and initial influenza infection but might not be sufficient to prevent the PR8 virus invasion and replication in the lung. Overall, the present study indicates that oral administration of attenuated S. Typhimurium can pre-stimulate multiple TLR pathways in mice to provide immediate early StIR against a lethal H1N1 virus challenge.
Keywords	agonists ; body weight changes ; chemical elements ; genes ; influenza ; Influenza A virus ; interferon-alpha ; interferon-beta ; intranasal administration ; lungs ; messenger RNA ; mice ; mononuclear leukocytes ; mortality ; nose ; oral administration ; Salmonella Typhimurium ; Toll-like receptor 4 ; Toll-like receptor 5 ; viruses
Language	English
Dates of publication	2017-03
Size	p. 1-6.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	753154-0
ISSN	1873-2542 ; 0378-1135
ISSN (online)	1873-2542
ISSN	0378-1135
DOI	10.1016/j.vetmic.2017.01.006
Database	NAL-Catalogue (AGRICOLA)

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Article: Protective effects of recombinant glycoprotein D based prime boost approach against duck enteritis virus in mice model

Aravind, S / C. Madhan Mohan / Nitin Machindra Kamble / R. Saravanan / Sanjeev Kumar Shukla / Satish S. Gaikwad / Sohini Dey

Microbial pathogenesis. 2015 Nov., v. 88

2015

Abstract: Duck virus enteritis, also known as duck plague, is an acute herpes viral infection of ducks caused by duck enteritis virus (DEV). The method of repeated immunization with a live attenuated vaccine has been used for the prevention and control of duck ... ...

Abstract	Duck virus enteritis, also known as duck plague, is an acute herpes viral infection of ducks caused by duck enteritis virus (DEV). The method of repeated immunization with a live attenuated vaccine has been used for the prevention and control of duck enteritis virus (DEV). However, the incidence of the disease in vaccinated flocks and latency reactivation are the major constraints in the present vaccination programme. The immunogenicity and protective efficacy afforded by intramuscular inoculation of plasmid DNA encoding DEV glycoprotein D (pCDNA-gD) followed by DEV gD expressed in Saccharomyces cerevisia (rgD) was assessed in a murine model. Compared with mice inoculated with DNA (pCDNA-gD) or protein (rgD) only, mice inoculated with the combination of gD DNA and protein had enhanced ELISA antibody titers to DEV and had accelerated clearance of virus following challenge infection. Furthermore, the highest levels of lymphocyte proliferation response, IL-4, IL-12 and IFN-γ production were induced following priming with the DNA vaccine and boosting with the rgD protein. For instance, the specially designed recombinant DEV vector vaccine would be the best choice to use in ducks. It offers an excellent solution to the low vaccination coverage rate in ducks. We expect that the application of this novel vaccine in the near future will greatly decrease the virus load in the environment and reduce outbreaks of DEV in ducks.
Keywords	Anatid herpesvirus 1 ; animal models ; antibodies ; duck plague ; ducks ; enzyme-linked immunosorbent assay ; flocks ; glycoproteins ; immunogenicity ; interferon-gamma ; interleukin-12 ; interleukin-4 ; live vaccines ; mice ; plasmids ; protective effect ; Saccharomyces ; vaccination ; vector vaccines ; viral load ; viruses
Language	English
Dates of publication	2015-11
Size	p. 78-86.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	632772-2
ISSN	1096-1208 ; 0882-4010
ISSN (online)	1096-1208
ISSN	0882-4010
DOI	10.1016/j.micpath.2015.07.008
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In stock of ZB MED Cologne/Königswinter

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In stock of ZB MED Cologne/Königswinter

Order via subito