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  1. Article: Obituary: Claus Nielsen 1938-2024.

    Heinze, Jürgen / Technau, Ulrich

    Frontiers in zoology

    2024  Volume 21, Issue 1, Page(s) 7

    Language English
    Publishing date 2024-03-12
    Publishing country England
    Document type Editorial
    ZDB-ID 2164409-3
    ISSN 1742-9994
    ISSN 1742-9994
    DOI 10.1186/s12983-024-00528-0
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  2. Article ; Online: Gastrulation and germ layer formation in the sea anemone Nematostella vectensis and other cnidarians.

    Technau, Ulrich

    Mechanisms of development

    2020  Volume 163, Page(s) 103628

    Abstract: Among the basally branching metazoans, cnidarians display well-defined gastrulation processes leading to a diploblastic body plan, consisting of an endodermal and an ectodermal cell layer. As the outgroup to all Bilateria, cnidarians are an interesting ... ...

    Abstract Among the basally branching metazoans, cnidarians display well-defined gastrulation processes leading to a diploblastic body plan, consisting of an endodermal and an ectodermal cell layer. As the outgroup to all Bilateria, cnidarians are an interesting group to investigate ancestral developmental mechanisms. Interestingly, all known gastrulation mechanisms known in Bilateria are already found in different species of Cnidaria. Here I review the morphogenetic processes found in different Cnidaria and focus on the investigation of the cellular and molecular mechanisms in the sea anemone Nematostella vectensis, which has been a major model organism among cnidarians for evolutionary developmental biology. Many of the genes involved in germ layer specification and morphogenetic processes in Bilateria are also found active during gastrulation of Nematostella and other cnidarians, suggesting an ancestral role of this process. The molecular analyses indicate a tight link between gastrulation and axis patterning processes by Wnt and FGF signaling. Interestingly, the endodermal layer displays many features of the mesodermal layer in Bilateria, while the pharyngeal ectoderm has an endodermal expression profile. Comparative analyses as well as experimental studies using embryonic aggregates suggest that minor differences in the gene regulatory networks allow the embryo to transition relatively easily from one mode of gastrulation to another.
    MeSH term(s) Animals ; Body Patterning/genetics ; Cnidaria/genetics ; Cnidaria/growth & development ; Ectoderm/growth & development ; Embryo, Nonmammalian/physiology ; Endoderm/growth & development ; Gastrulation/genetics ; Gene Expression Regulation, Developmental/genetics ; Gene Regulatory Networks/genetics ; Germ Layers/growth & development ; Germ Layers/metabolism ; Mesoderm/growth & development ; Sea Anemones/genetics ; Sea Anemones/growth & development ; Signal Transduction/genetics
    Language English
    Publishing date 2020-06-27
    Publishing country Ireland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1055986-3
    ISSN 1872-6356 ; 0925-4773
    ISSN (online) 1872-6356
    ISSN 0925-4773
    DOI 10.1016/j.mod.2020.103628
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  3. Article ; Online: Evolution: Directives from Sea Anemone Hox Genes.

    Technau, Ulrich / Genikhovich, Grigory

    Current biology : CB

    2018  Volume 28, Issue 22, Page(s) R1303–R1305

    Abstract: A new study in a sea anemone shows that, as in bilaterian animals, an axis-patterning Hox code exists in cnidarians. Surprisingly, it acts along the directive axis, orthogonal to the oral-aboral axis. ...

    Abstract A new study in a sea anemone shows that, as in bilaterian animals, an axis-patterning Hox code exists in cnidarians. Surprisingly, it acts along the directive axis, orthogonal to the oral-aboral axis.
    MeSH term(s) Animals ; Body Patterning/genetics ; Evolution, Molecular ; Gene Expression Regulation, Developmental/genetics ; Genes, Homeobox/genetics ; Models, Biological ; Phylogeny ; Sea Anemones/genetics
    Language English
    Publishing date 2018-11-19
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1071731-6
    ISSN 1879-0445 ; 0960-9822
    ISSN (online) 1879-0445
    ISSN 0960-9822
    DOI 10.1016/j.cub.2018.09.040
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  4. Book ; Online ; Thesis: Unraveling the interactome of chromatin regulators that block reprogramming

    Baytek, Gülkiz [Verfasser] / Sommer, Thomas [Gutachter] / Tursun, Baris [Gutachter] / Technau, Ulrich [Gutachter]

    2022  

    Author's details Gülkiz Baytek ; Gutachter: Thomas Sommer, Baris Tursun, Ulrich Technau
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language English
    Publisher Humboldt-Universität zu Berlin
    Publishing place Berlin
    Document type Book ; Online ; Thesis
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  5. Article ; Online: On the evolution of bilaterality.

    Genikhovich, Grigory / Technau, Ulrich

    Development (Cambridge, England)

    2017  Volume 144, Issue 19, Page(s) 3392–3404

    Abstract: Bilaterality - the possession of two orthogonal body axes - is the name-giving trait of all bilaterian animals. These body axes are established during early embryogenesis and serve as a three-dimensional coordinate system that provides crucial spatial ... ...

    Abstract Bilaterality - the possession of two orthogonal body axes - is the name-giving trait of all bilaterian animals. These body axes are established during early embryogenesis and serve as a three-dimensional coordinate system that provides crucial spatial cues for developing cells, tissues, organs and appendages. The emergence of bilaterality was a major evolutionary transition, as it allowed animals to evolve more complex body plans. Therefore, how bilaterality evolved and whether it evolved once or several times independently is a fundamental issue in evolutionary developmental biology. Recent findings from non-bilaterian animals, in particular from Cnidaria, the sister group to Bilateria, have shed new light into the evolutionary origin of bilaterality. Here, we compare the molecular control of body axes in radially and bilaterally symmetric cnidarians and bilaterians, identify the minimal set of traits common for Bilateria, and evaluate whether bilaterality arose once or more than once during evolution.
    MeSH term(s) Animals ; Biological Evolution ; Body Patterning ; Bone Morphogenetic Proteins/metabolism ; Humans ; Phylogeny ; Signal Transduction
    Chemical Substances Bone Morphogenetic Proteins
    Language English
    Publishing date 2017-09-30
    Publishing country England
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 90607-4
    ISSN 1477-9129 ; 0950-1991
    ISSN (online) 1477-9129
    ISSN 0950-1991
    DOI 10.1242/dev.141507
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  6. Article: Updated single cell reference atlas for the starlet anemone Nematostella vectensis.

    Cole, Alison G / Steger, Julia / Hagauer, Julia / Denner, Andreas / Ferrer Murguia, Patricio / Knabl, Paul / Narayanaswamy, Sanjay / Wick, Brittney / Montenegro, Juan D / Technau, Ulrich

    Frontiers in zoology

    2024  Volume 21, Issue 1, Page(s) 8

    Abstract: Background: The recent combination of genomics and single cell transcriptomics has allowed to assess a variety of non-conventional model organisms in much more depth. Single cell transcriptomes can uncover hidden cellular complexity and cell lineage ... ...

    Abstract Background: The recent combination of genomics and single cell transcriptomics has allowed to assess a variety of non-conventional model organisms in much more depth. Single cell transcriptomes can uncover hidden cellular complexity and cell lineage relationships within organisms. The recent developmental cell atlases of the sea anemone Nematostella vectensis, a representative of the basally branching Cnidaria, has provided new insights into the development of all cell types (Steger et al Cell Rep 40(12):111370, 2022; Sebé-Pedrós et al. Cell 173(6):1520-1534.e20). However, the mapping of the single cell reads still suffers from relatively poor gene annotations and a draft genome consisting of many scaffolds.
    Results: Here we present a new wildtype resource of the developmental single cell atlas, by re-mapping of sequence data first published in Steger et al. (2022) and Cole et al. (Nat Commun 14(1):1747, 2023), to the new chromosome-level genome assembly and corresponding gene models in Zimmermann et al. (Nat Commun 14, 8270 (2023). https://doi.org/10.1038/s41467-023-44080-7 ). We expand the pre-existing dataset through the incorporation of additional sequence data derived from the capture and sequencing of cell suspensions from four additional samples: 24 h gastrula, 2d planula, an inter-parietal region of the bodywall from a young unsexed animal, and another adult mesentery from a mature male animal.
    Conclusion: Our analyses of the full cell-state inventory provide transcriptomic signatures for 127 distinct cell states, of which 47 correspond to neuroglandular subtypes. We also identify two distinct putatively immune-related transcriptomic profiles that segregate between the inner and outer cell layers. Furthermore, the new gene annotation Nv2 has markedly improved the mapping on the single cell transcriptome data and will therefore be of great value for the community and anyone using the dataset.
    Language English
    Publishing date 2024-03-18
    Publishing country England
    Document type Journal Article
    ZDB-ID 2164409-3
    ISSN 1742-9994
    ISSN 1742-9994
    DOI 10.1186/s12983-024-00529-z
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  7. Article ; Online: Meganuclease-assisted generation of stable transgenics in the sea anemone Nematostella vectensis.

    Renfer, Eduard / Technau, Ulrich

    Nature protocols

    2017  Volume 12, Issue 9, Page(s) 1844–1854

    Abstract: The sea anemone Nematostella vectensis is a model system used by a rapidly growing research community for comparative genomics, developmental biology and ecology. Here, we describe a microinjection procedure for creating stable transgenic lines in ... ...

    Abstract The sea anemone Nematostella vectensis is a model system used by a rapidly growing research community for comparative genomics, developmental biology and ecology. Here, we describe a microinjection procedure for creating stable transgenic lines in Nematostella based on meganuclease (I-SceI)-assisted integration of a transgenic cassette into the genome. The procedure describes the preparation of the reagents, microinjection of the transgenesis vector and the husbandry of transgenic animals. The microinjection setup differs from those of previously published protocols by the use of a holding capillary mounted on an inverted fluorescence microscope. In one session of injections, a single researcher can microinject up to 1,300 zygotes with a reporter construct digested with the meganuclease I-SceI. Under optimal conditions, fully transgenic heterozygous F1 animals can be obtained within 4-5 months of the injections, with a germ-line transmission efficiency of ∼3%. The method is versatile and, after a short training phase, can be carried out by any researcher with basic training in molecular biology. Flexibility of construct design enables this method to be used for numerous applications, including the functional dissection of cis-regulatory elements, subcellular localization of proteins, detection of protein-binding partners, ectopic expression of genes of interest, lineage tracing and cell-type-specific reporter gene expression.
    MeSH term(s) Animals ; Animals, Genetically Modified/genetics ; Animals, Genetically Modified/physiology ; Cloning, Molecular ; Deoxyribonucleases, Type II Site-Specific/metabolism ; Equipment Design ; Genetic Engineering/methods ; Microinjections/instrumentation ; Microinjections/methods ; Saccharomyces cerevisiae Proteins/metabolism ; Sea Anemones/genetics ; Sea Anemones/physiology
    Chemical Substances Saccharomyces cerevisiae Proteins ; SCEI protein, S cerevisiae (EC 3.1.21.-) ; Deoxyribonucleases, Type II Site-Specific (EC 3.1.21.4)
    Language English
    Publishing date 2017-08-17
    Publishing country England
    Document type Journal Article
    ZDB-ID 2244966-8
    ISSN 1750-2799 ; 1754-2189
    ISSN (online) 1750-2799
    ISSN 1754-2189
    DOI 10.1038/nprot.2017.075
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  8. Article ; Online: Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian Nematostella vectensis.

    Rentzsch, Fabian / Renfer, Eduard / Technau, Ulrich

    Methods in molecular biology (Clifton, N.J.)

    2019  Volume 2047, Page(s) 45–57

    Abstract: Neurons often display complex morphologies with long and fine processes that can be difficult to visualize, in particular in living animals. Transgenic reporter lines in which fluorescent proteins are expressed in defined populations of neurons are ... ...

    Abstract Neurons often display complex morphologies with long and fine processes that can be difficult to visualize, in particular in living animals. Transgenic reporter lines in which fluorescent proteins are expressed in defined populations of neurons are important tools that can overcome these difficulties. By using membrane-attached fluorescent proteins, such reporter transgenes can identify the complete outline of subsets of neurons or they can highlight the subcellular localization of fusion proteins, for example at pre- or postsynaptic sites. The relative stability of fluorescent proteins furthermore allows the tracing of the progeny of cells over time and can therefore provide information about potential roles of the gene whose regulatory elements are controlling the expression of the fluorescent protein. Here we describe the generation of transgenic reporter lines in the sea anemone Nematostella vectensis, a cnidarian model organism for studying the evolution of developmental processes. We also provide an overview of existing transgenic Nematostella lines that have been used to study conserved and derived aspects of nervous system development.
    MeSH term(s) Animals ; Animals, Genetically Modified/growth & development ; Genes, Reporter ; Luminescent Proteins/genetics ; Luminescent Proteins/metabolism ; Nervous System/growth & development ; Neurogenesis ; Sea Anemones/genetics ; Sea Anemones/growth & development
    Chemical Substances Luminescent Proteins
    Language English
    Publishing date 2019-08-20
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-9732-9_3
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  9. Article ; Online: Evolutionary biology: Small regulatory RNAs pitch in.

    Technau, Ulrich

    Nature

    2008  Volume 455, Issue 7217, Page(s) 1184–1185

    MeSH term(s) Animals ; Evolution, Molecular ; Gene Expression Regulation ; Humans ; MicroRNAs/genetics ; Phylogeny ; RNA, Small Interfering/genetics ; Sea Anemones/genetics
    Chemical Substances MicroRNAs ; RNA, Small Interfering
    Language English
    Publishing date 2008-10-30
    Publishing country England
    Document type Comment ; News
    ZDB-ID 120714-3
    ISSN 1476-4687 ; 0028-0836
    ISSN (online) 1476-4687
    ISSN 0028-0836
    DOI 10.1038/4551184a
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  10. Article ; Online: Genomics and development of Nematostella vectensis and other anthozoans.

    Rentzsch, Fabian / Technau, Ulrich

    Current opinion in genetics & development

    2016  Volume 39, Page(s) 63–70

    Abstract: Due to their rather simple body plan with only few organs and a low number of cell types, cnidarians have long been recognized as an important animal group for evolutionary comparisons of animal body plans. Recent studies have shown, however, that the ... ...

    Abstract Due to their rather simple body plan with only few organs and a low number of cell types, cnidarians have long been recognized as an important animal group for evolutionary comparisons of animal body plans. Recent studies have shown, however, that the genomes of cnidarians and their epigenetic and posttranscriptional regulation are more complex than their morphology might suggest. How these complex genomes are deployed during embryonic development is an open question. With a focus on the sea anemone Nematostella vectensis we describe new findings about the development of the nervous system from neural progenitor cells and how Wnt and BMP signalling control axial patterning. These studies show that beyond evolutionary comparisons, cnidarian model organisms can provide new insights into generic questions in developmental biology.
    MeSH term(s) Animals ; Gene Expression Regulation, Developmental ; Genome/genetics ; Genomics ; Neural Stem Cells ; Sea Anemones/genetics ; Sea Anemones/growth & development ; Signal Transduction/genetics
    Language English
    Publishing date 2016-06-16
    Publishing country England
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 1077312-5
    ISSN 1879-0380 ; 0959-437X
    ISSN (online) 1879-0380
    ISSN 0959-437X
    DOI 10.1016/j.gde.2016.05.024
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