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Article: Bu-Yin-Qian-Zheng Formula Ameliorates MPP

Ma, Hao-Jie / Gai, Cong / Chai, Yuan / Feng, Wan-Di / Cheng, Cui-Cui / Zhang, Jin-Kun / Zhang, Yu-Xin / Yang, Lu-Ping / Guo, Zhen-Yu / Gao, Yu-Shan / Sun, Hong-Mei

Frontiers in pharmacology

2020 Volume 11, Page(s) 577017

Abstract: As a typical traditional Chinese medicine, Bu-Yin-Qian-Zheng Formula (BYQZF) has been shown to have ...

Abstract	As a typical traditional Chinese medicine, Bu-Yin-Qian-Zheng Formula (BYQZF) has been shown to have neuroprotective effects in patients with Parkinson's disease (PD), particularly by ameliorating mitochondrial dysfunction and regulating expression of the parkin protein. However, the underlying mechanisms by which BYQZF affects mitochondrial function through parkin are unclear. Accordingly, in this study, we evaluated the mechanisms by which BYQZF ameliorates mitochondrial dysfunction through parkin in PD. We constructed a parkin-knockdown cell model and performed fluorescence microscopy to observe transfected SH-SY5Y cells. Quantitative real-time reverse transcription polymerase chain reaction and western blotting were conducted to detect the mRNA and protein expression levels of parkin. Additionally, we evaluated the cell survival rates, ATP levels, mitochondrial membrane potential (ΔΨm), mitochondrial morphology, parkin protein expression, PINK1 protein expression, and mitochondrial fusion and fission protein expression after treatment with MPP
Language	English
Publishing date	2020-12-18
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2020.577017
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Article: Qian Yang Yu Yin Granule Improves Renal Injury of Hypertension by Regulating Metabolic Reprogramming Mediated by HIF-1α/PKM2 Positive Feedback Loop.

Qian, Lichao / Ren, Shuai / Xu, Zhongchi / Zheng, Yawei / Wu, Lihua / Yang, Ying / Wang, Yixuan / Li, Jie / Yan, Shihai / Fang, Zhuyuan

Frontiers in pharmacology

2021 Volume 12, Page(s) 667433

Abstract: ... nephropathy. In this study, the effects of Qian Yang Yu Yin granule (QYYY) on spontaneously hypertensive rats ...

Abstract	Protection against hypoxia injury is an important therapeutic strategy for treating hypertensive nephropathy. In this study, the effects of Qian Yang Yu Yin granule (QYYY) on spontaneously hypertensive rats fed with high salt diet and HEK293T cells exposed to hypoxia were investigated. After eight weeks' treatment of QYYY, blood pressure, serum creatinine, serum cystatin C, blood urea nitrogen, urinary β2-microglobulin, urinary N-acetyl-β-glucosaminidase, and urinary microalbumin were assessed. The changes of hypoxia-inducible factor-1α (HIF-1α), pyruvate kinase M2 (PKM2), glucose transport 1 (GLUT1), lactate dehydrogenase A (LDH-A), connective tissue growth factor (CTGF), transforming growth factor-β1 (TGF-β1), ATP, lactate, pyruvate, and pathology were also assessed
Language	English
Publishing date	2021-06-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2021.667433
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Article ; Online: Study on Fu-Fang-Jin-Qian-Cao Inhibiting Autophagy in Calcium Oxalate-induced Renal Injury by UHPLC/Q-TOF-MS-based Metabonomics and Network Pharmacology Approaches.

Liu, Wen-Rui / Li, Mao-Ting / Zhou, Qi / Gao, Song-Yan / Hou, Jie-Bin / Yang, Guo-Bin / Liu, Nan-Mei / Jia-Yan / Yu, Jian-Peng / Cheng, Jin / Guo, Zhi-Yong

Combinatorial chemistry & high throughput screening

2024 Volume 27, Issue 1, Page(s) 90–100

Abstract: Introduction: Fu-Fang-Jin-Qian-Cao is a Chinese herbal preparation used to treat urinary calculi ... Fu-Fang-Jin-Qian-Cao can protect renal tubular epithelial cells from calcium oxalateinduced renal ... pharmacology to study the mechanism of Fu-Fang-Jin-Qian-Cao inhibiting autophagy in calcium oxalate-induced ...

Abstract	Introduction: Fu-Fang-Jin-Qian-Cao is a Chinese herbal preparation used to treat urinary calculi. Fu-Fang-Jin-Qian-Cao can protect renal tubular epithelial cells from calcium oxalateinduced renal injury by inhibiting ROS-mediated autopathy. The mechanism still needs further exploration. Metabonomics is a new subject; the combination of metabolomics and network pharmacology can find pathways for drugs to act on targets more efficiently. Methods: Comprehensive metabolomics and network pharmacology to study the mechanism of Fu-Fang-Jin-Qian-Cao inhibiting autophagy in calcium oxalate-induced renal injury. Based on UHPLC-Q-TOF-MS, combined with biochemical analysis, a mice model of Calcium oxalateinduced renal injury was established to study the therapeutic effect of Fu-Fang-Jin-Qian-Cao. Based on the network pharmacology, the target signaling pathway and the protective effect of Fu- Fang-Jin-Qian-Cao on Calcium oxalate-induced renal injury by inhibiting autophagy were explored. Autophagy-related proteins LC3-II, BECN1, ATG5, and ATG7 were studied by immunohistochemistry. Results: Combining network pharmacology and metabolomics, 50 differential metabolites and 2482 targets related to these metabolites were found. Subsequently, the targets enriched in PI3KAkt, MAPK and Ras signaling pathways. LC3-II, BECN1, ATG5 and ATG7 were up-regulated in Calcium oxalate-induced renal injury. All of them could be reversed after the Fu-Fang-Jin-Qian- Cao treatment. Conclusions: Fu-Fang-Jin-Qian-Cao can reverse ROS-induced activation of the MAPK signaling pathway and inhibition of the PI3K-Akt signaling pathway, thereby reducing autophagy damage of renal tubular epithelial cells in Calcium oxalate-induced renal injury.
MeSH term(s)	Mice ; Animals ; Calcium Oxalate/metabolism ; Calcium Oxalate/pharmacology ; Calcium/metabolism ; Chromatography, High Pressure Liquid ; Network Pharmacology ; Phosphatidylinositol 3-Kinases/metabolism ; Reactive Oxygen Species/metabolism ; Kidney/metabolism ; Autophagy ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/metabolism
Chemical Substances	Calcium Oxalate (2612HC57YE) ; Calcium (SY7Q814VUP) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Reactive Oxygen Species ; Drugs, Chinese Herbal
Language	English
Publishing date	2024-01-12
Publishing country	United Arab Emirates
Document type	Journal Article
ZDB-ID	2064785-2
ISSN	1875-5402 ; 1386-2073
ISSN (online)	1875-5402
ISSN	1386-2073
DOI	10.2174/1386207326666230515151302
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Article ; Online: Reply to the Letter from Qian Ren and Yanjun Wang About the Article, "LI-RADS Morphological Type Predicts Prognosis of Patients with Hepatocellular Carcinoma After Radical Resection".

Zhang, Chunhui / Yang, Rui / Wang, Xinxin / Tao, Yuqing / Tang, Shuli / Tian, Zhennan / Zhou, Yang

Annals of surgical oncology

2023 Volume 31, Issue 3, Page(s) 1849–1850

MeSH term(s)	Humans ; Carcinoma, Hepatocellular/pathology ; Liver Neoplasms/pathology ; Contrast Media ; Magnetic Resonance Imaging ; Prognosis
Chemical Substances	Contrast Media
Language	English
Publishing date	2023-11-13
Publishing country	United States
Document type	Letter ; Comment
ZDB-ID	1200469-8
ISSN	1534-4681 ; 1068-9265
ISSN (online)	1534-4681
ISSN	1068-9265
DOI	10.1245/s10434-023-14557-2
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Article ; Online: Effects of Fusu mixture (Wen-Shen-Qian-Yang Method) on sepsis-induced acute respiratory distress syndrome.

Zhang, Li / Long, Kunlan / Wang, Chunxia / Zhang, Xuemei / Yang, Hongjing / Chen, Jun / Li, Xue / Gao, Peiyang / Zhang, Song

Medicine

2020 Volume 99, Issue 29, Page(s) e21066

Abstract: Introduction: Sepsis is the most common etiology of acute lung injury (ALI) or acute respiratory distress syndrome (ARDS). Capillary leakage caused by lung endothelial injury is the central cause of ARDS. The results of research in modern medicine in ... ...

Abstract	Introduction: Sepsis is the most common etiology of acute lung injury (ALI) or acute respiratory distress syndrome (ARDS). Capillary leakage caused by lung endothelial injury is the central cause of ARDS. The results of research in modern medicine in reducing endothelial damage and restoring endothelial functions are limited. In the previous clinical observations, we found that the Fusu mixture not only improves the clinical symptoms but also reduces the leakage of pulmonary capillaries. Therefore, the purpose of this study is to determine the clinical efficacy of the Fusu mixture combined with Western medicine in the treatment of ARDS caused by sepsis and to explore the mechanism of traditional Chinese medicine. Methods: This is a prospective, single-center, randomized, single-blind, and controlled clinical study involving 620 eligible patients. The patients will be randomly divided into 2 groups: the Western medicine treatment group and the combination of Chinese and Western medicine treatment group. After 14 days of intervention, the clinical efficacy and safety of the Fusu mixture on sepsis-induced ARDS patients will be observed. The primary outcome will be measured as 28-day mortality. The secondary outcome indices include inflammatory markers (CRP, PCT, IL-6, TNF - α), APACHE II score, SOFA score, days without a ventilator, blood gas analysis (Lac, PaO2 / FiO2), intensive care unit hospital stay time, intensive care unit mortality. Simultaneously, the analysis of the exploratory results will be carried out to analyze the possible mechanism of Fusu mixture in the treatment of sepsis-induced ARDS by the high-throughput sequencing and bioinformatics. Discussion: The purpose of this study is to evaluate the clinical efficacy of Fusu mixture in the treatment of sepsis-induced ARDS and explore its possible mechanism of action. If successful, it will provide evidence-based adjuvant therapy for the clinical treatment of ARDS.
MeSH term(s)	Adult ; Blood Gas Analysis ; Capillary Leak Syndrome/complications ; Capillary Leak Syndrome/etiology ; Female ; Humans ; Male ; Medicine, Chinese Traditional/methods ; Medicine, Chinese Traditional/standards ; Middle Aged ; Prospective Studies ; Respiratory Distress Syndrome/drug therapy ; Respiratory Distress Syndrome/etiology ; Respiratory Distress Syndrome/physiopathology ; Sepsis/complications ; Sepsis/drug therapy ; Sepsis/physiopathology ; Single-Blind Method ; Treatment Outcome
Language	English
Publishing date	2020-04-16
Publishing country	United States
Document type	Journal Article ; Randomized Controlled Trial
ZDB-ID	80184-7
ISSN	1536-5964 ; 0025-7974
ISSN (online)	1536-5964
ISSN	0025-7974
DOI	10.1097/MD.0000000000021066
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Article ; Online: Evaluation of the antioxidant and endothelial protective effects of Lysimachia christinae Hance (Jin Qian Cao) extract fractions.

Wu, Ning-Hua / Ke, Zhi-Qiang / Wu, Shan / Yang, Xiao-Song / Chen, Qing-Jie / Huang, Sheng-Tang / Liu, Chao

BMC complementary and alternative medicine

2018 Volume 18, Issue 1, Page(s) 128

Abstract: Background: Lysimachia christinae Hance is a traditional Chinese medicine with diuretic, detumescent, and detoxifying effects. Our aimed to optimize the extraction protocol to maximize the yield of flavonoids from Lysimachia christinae Hance, and ... ...

Abstract	Background: Lysimachia christinae Hance is a traditional Chinese medicine with diuretic, detumescent, and detoxifying effects. Our aimed to optimize the extraction protocol to maximize the yield of flavonoids from Lysimachia christinae Hance, and evaluate the pharmacological activities of four fractions, namely, petroleum ether (PE), ethyl acetate (EA), n-butanol (NB), and aqueous (AQ) fractions, of the ethanolic extract of Lysimachia christinae Hance. Methods: The flavonoid monomers in the crude extract were characterized via high performance liquid chromatography (HPLC), were used as markers for extract quality control and standardization. The total flavonoid, total phenolic, and total polysaccharide contents of each fraction were determined by spectrophotometry. Further, the in vitro free radical (diphenylpicrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), superoxide, and hydroxyl radicals) scavenging activities, and antioxidant capacity in endothelial cells were evaluated for each fraction. Results: After optimizing the extraction protocol to maximize the total flavonoid yield from L. christinae Hance, the NB fractions had the highest total flavonoid (39.4 ± 4.55 mg RE/g), total phenolic (41.1 ± 3.07 mg GAE/g) and total polysaccharide (168.1 ± 7.07 mg GE/g); In addition, the NB fraction of the ethanolic extract of L. christinae Hance reveal the strongest radical-scavenging activity, antioxidant activity and protective effects against H Conclusions: Among the four fractions of L. christinae Hance, the NB fraction showed the most potent antioxidant and endothelial protective effects, which may be attributed to its high flavonoid, phenolic contents and optimal portfolio of different active ingredients of NB fractions of the ethanolic extract of L. christinae Hance. This study might improve our understanding of the pharmacological activities of L. christinae Hance, thereby facilitating its use in disease prevention and treatment.
MeSH term(s)	Antioxidants/chemistry ; Antioxidants/pharmacology ; Cell Survival/drug effects ; Flavonoids/analysis ; Human Umbilical Vein Endothelial Cells ; Humans ; Oxidative Stress/drug effects ; Phenols/analysis ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Polysaccharides/analysis ; Primulaceae/chemistry
Chemical Substances	Antioxidants ; Flavonoids ; Phenols ; Plant Extracts ; Polysaccharides
Language	English
Publishing date	2018-04-10
Publishing country	England
Document type	Journal Article
ZDB-ID	2050429-9
ISSN	1472-6882 ; 1472-6882
ISSN (online)	1472-6882
ISSN	1472-6882
DOI	10.1186/s12906-018-2157-1
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Article ; Online: Noise does not equal bias in assessing the evolutionary history of the angiosperm flora of China: A response to Qian (2019)

Lu, Li‐Min / Hu, Hai‐Hua / Peng, Dan‐Xiao / Liu, Bing / Ye, Jian‐Fei / Yang, Tuo / Li, Hong‐Lei / Sun, Miao / Smith, Stephen A. / Soltis, Pamela S. / Soltis, Douglas E. / Chen, Zhi‐Duan

Journal of biogeography. 2020 Oct., v. 47, no. 10 p.2286-2291

2020

Abstract: In response to our paper on the evolutionary history of the Chinese flora, Qian suggests ... al (2018). Here, we consider Qian's specific criticisms, explore the extent of uncertainty ...

Abstract	In response to our paper on the evolutionary history of the Chinese flora, Qian suggests that certain features of the divergence time estimation employed might have led to biased conclusions in Lu et al (2018). Here, we consider Qian's specific criticisms, explore the extent of uncertainty in the data and demonstrate that (i) no systematic bias toward dates that are too young or too old is detected in Lu et al.; (ii) constraint of the crown age of angiosperms does not bias the generic ages estimated by Lu et al.; and (iii) ages derived from the Chinese regional phylogeny do not bias the conclusions reported by Lu et al. All these analyses confirm that the conclusions reported previously are robust. We argue that, like many large‐scale biodiversity analyses, sources of noise in divergence time estimation are to be expected, but these should not be confused with bias.
Keywords	Angiospermae ; biodiversity ; biogeography ; flora ; phylogeny ; uncertainty ; China
Language	English
Dates of publication	2020-10
Size	p. 2286-2291.
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	NAL-AP-2-clean ; LETTER
ZDB-ID	188963-1
ISSN	0305-0270
ISSN	0305-0270
DOI	10.1111/jbi.13947
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Game theory in epigenetic reprogramming: Comment on: "Epigenetic game theory: How to compute the epigenetic control of maternal-to-zygotic transition" by Qian Wang et al.

Hsu, Fei-Man / Chen, Pao-Yang

Physics of life reviews

2017 Volume 20, Page(s) 143–145

MeSH term(s)	Epigenomics ; Game Theory ; Humans ; Zygote
Language	English
Publishing date	2017
Publishing country	Netherlands
Document type	Journal Article ; Comment
ZDB-ID	2148883-6
ISSN	1873-1457 ; 1571-0645
ISSN (online)	1873-1457
ISSN	1571-0645
DOI	10.1016/j.plrev.2017.01.005
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Article ; Online: Evaluation of the antioxidant and endothelial protective effects of Lysimachia christinae Hance (Jin Qian Cao) extract fractions

Ning-hua Wu / Zhi-qiang Ke / Shan Wu / Xiao-song Yang / Qing-jie Chen / Sheng-tang Huang / Chao Liu

BMC Complementary and Alternative Medicine, Vol 18, Iss 1, Pp 1-

2018 Volume 7

Abstract: Abstract Background Lysimachia christinae Hance is a traditional Chinese medicine with diuretic, detumescent, and detoxifying effects. Our aimed to optimize the extraction protocol to maximize the yield of flavonoids from Lysimachia christinae Hance, and ...

Abstract	Abstract Background Lysimachia christinae Hance is a traditional Chinese medicine with diuretic, detumescent, and detoxifying effects. Our aimed to optimize the extraction protocol to maximize the yield of flavonoids from Lysimachia christinae Hance, and evaluate the pharmacological activities of four fractions, namely, petroleum ether (PE), ethyl acetate (EA), n-butanol (NB), and aqueous (AQ) fractions, of the ethanolic extract of Lysimachia christinae Hance. Methods The flavonoid monomers in the crude extract were characterized via high performance liquid chromatography (HPLC), were used as markers for extract quality control and standardization. The total flavonoid, total phenolic, and total polysaccharide contents of each fraction were determined by spectrophotometry. Further, the in vitro free radical (diphenylpicrylhydrazyl, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), superoxide, and hydroxyl radicals) scavenging activities, and antioxidant capacity in endothelial cells were evaluated for each fraction. Results After optimizing the extraction protocol to maximize the total flavonoid yield from L. christinae Hance, the NB fractions had the highest total flavonoid (39.4 ± 4.55 mg RE/g), total phenolic (41.1 ± 3.07 mg GAE/g) and total polysaccharide (168.1 ± 7.07 mg GE/g); In addition, the NB fraction of the ethanolic extract of L. christinae Hance reveal the strongest radical-scavenging activity, antioxidant activity and protective effects against H2O2-induced injury in HUVECs. Conclusions Among the four fractions of L. christinae Hance, the NB fraction showed the most potent antioxidant and endothelial protective effects, which may be attributed to its high flavonoid, phenolic contents and optimal portfolio of different active ingredients of NB fractions of the ethanolic extract of L. christinae Hance. This study might improve our understanding of the pharmacological activities of L. christinae Hance, thereby facilitating its use in disease prevention and treatment.
Keywords	Lysimachia christinae Hance ; Radical-scavenging ; Antioxidant ; HUVECs ; Flavonoids ; Other systems of medicine ; RZ201-999
Subject code	500
Language	English
Publishing date	2018-04-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Evaluation of the antioxidant and endothelial protective effects of Lysimachia christinae Hance (Jin Qian Cao) extract fractions

Wu, Ning-hua / Ke, Zhi-qiang / Wu, Shan / Yang, Xiao-song / Chen, Qing-jie / Huang, Sheng-tang / Liu, Chao

BMC Complement Altern Med. 2018 Dec., v. 18, no. 1 p.128-128

2018

Abstract: BACKGROUND: Lysimachia christinae Hance is a traditional Chinese medicine with diuretic, detumescent, and detoxifying effects. Our aimed to optimize the extraction protocol to maximize the yield of flavonoids from Lysimachia christinae Hance, and ... ...

Abstract	BACKGROUND: Lysimachia christinae Hance is a traditional Chinese medicine with diuretic, detumescent, and detoxifying effects. Our aimed to optimize the extraction protocol to maximize the yield of flavonoids from Lysimachia christinae Hance, and evaluate the pharmacological activities of four fractions, namely, petroleum ether (PE), ethyl acetate (EA), n-butanol (NB), and aqueous (AQ) fractions, of the ethanolic extract of Lysimachia christinae Hance. METHODS: The flavonoid monomers in the crude extract were characterized via high performance liquid chromatography (HPLC), were used as markers for extract quality control and standardization. The total flavonoid, total phenolic, and total polysaccharide contents of each fraction were determined by spectrophotometry. Further, the in vitro free radical (diphenylpicrylhydrazyl, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), superoxide, and hydroxyl radicals) scavenging activities, and antioxidant capacity in endothelial cells were evaluated for each fraction. RESULTS: After optimizing the extraction protocol to maximize the total flavonoid yield from L. christinae Hance, the NB fractions had the highest total flavonoid (39.4 ± 4.55 mg RE/g), total phenolic (41.1 ± 3.07 mg GAE/g) and total polysaccharide (168.1 ± 7.07 mg GE/g); In addition, the NB fraction of the ethanolic extract of L. christinae Hance reveal the strongest radical-scavenging activity, antioxidant activity and protective effects against H₂O₂-induced injury in HUVECs. CONCLUSIONS: Among the four fractions of L. christinae Hance, the NB fraction showed the most potent antioxidant and endothelial protective effects, which may be attributed to its high flavonoid, phenolic contents and optimal portfolio of different active ingredients of NB fractions of the ethanolic extract of L. christinae Hance. This study might improve our understanding of the pharmacological activities of L. christinae Hance, thereby facilitating its use in disease prevention and treatment.
Keywords	Lysimachia ; Oriental traditional medicine ; antioxidant activity ; antioxidants ; butanol ; calcium oxide ; complement ; disease prevention ; ethyl acetate ; flavonoids ; free radicals ; high performance liquid chromatography ; petroleum ; polysaccharides ; quality control ; spectroscopy
Language	English
Dates of publication	2018-12
Size	p. 128.
Publishing place	BioMed Central
Document type	Article ; Online
Note	Resource is Open Access
ZDB-ID	2050429-9
ISSN	1472-6882
ISSN	1472-6882
DOI	10.1186/s12906-018-2157-1
Database	NAL-Catalogue (AGRICOLA)

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