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  1. Article ; Online: Extended use or reuse of N95 respirators during COVID-19 pandemic: An overview of national regulatory authority recommendations.

    Kobayashi, Leticia Mitiko / Marins, Bianca Ramos / Costa, Patrícia Cristina Dos Santos / Perazzo, Hugo / Castro, Rodolfo

    Infection control and hospital epidemiology

    2020  Volume 41, Issue 11, Page(s) 1364–1366

    MeSH term(s) Betacoronavirus ; COVID-19 ; Coronavirus ; Coronavirus Infections/epidemiology ; Hong Kong ; Humans ; Infection Control ; Masks ; Pandemics/prevention & control ; Pneumonia, Viral ; SARS-CoV-2
    Keywords covid19
    Language English
    Publishing date 2020-04-22
    Publishing country United States
    Document type Journal Article ; Comment
    ZDB-ID 639378-0
    ISSN 1559-6834 ; 0195-9417 ; 0899-823X
    ISSN (online) 1559-6834
    ISSN 0195-9417 ; 0899-823X
    DOI 10.1017/ice.2020.173
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1714: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  2. Article ; Online: Short communication: Feasibility of dengue vaccine to infect different human cell lines: An alternative potency test using HEK293T cells.

    Faria de Carvalho, Renata / de Siqueira Penna Quintaes, Lucas / de Cássia de Souza Su, Thaís / Mitiko Kobayashi, Leticia / Martins de Almeida Nogueira, Ana Cristina

    PloS one

    2022  Volume 17, Issue 5, Page(s) e0267653

    Abstract: Dengue is caused by an arbovirus that belongs to the Flaviviridae family and there are four distinct, but close related, circulating serotypes. Dengue disease is of great importance for global public health, with vaccination being its main prophylactic ... ...

    Abstract Dengue is caused by an arbovirus that belongs to the Flaviviridae family and there are four distinct, but close related, circulating serotypes. Dengue disease is of great importance for global public health, with vaccination being its main prophylactic measure. However, there is a paucity of biological models for evaluating tetravalent dengue vaccines. The aim of this study was to evaluate the susceptibility of human cell lines HEK293T and THP-1 to a commercial dengue vaccine and test the feasibility of this approach in the development of a potency assay with human cell lines, as a methodological alternative to the golden standard potency assay with VERO cells. In this context, we used a batch of the commercial vaccine Dengvaxia® (CYD-TDV) for the infection tests. We evaluated the presence of the vaccine virus in THP-1 cells, differentiated into macrophages (dTHP-1), and in HEK293T by confocal microscopy, using 4G2 pan-flavivirus antibody. Vaccine infectivity and potency were determined by immunocolorimetric assay using monoclonal antibodies specific for each serotype. The results indicated that the human strain HEK293T was responsive to the tetravalent vaccine, as shown by the presence of virus particles in the cell cytoplasm in a pattern similar to the one observed with VERO cells. Moreover, it was possible to determine the infectivity and potency values of each vaccine virus serotype in the HEK293T, with serotype 4 prevailing over the others. Thus, the human cell line HEK293T provides a potential candidate to be used in assays to determine potency and identity of tetravalent dengue vaccines.
    MeSH term(s) Animals ; Antibodies, Neutralizing ; Antibodies, Viral ; Chlorocebus aethiops ; Dengue/prevention & control ; Dengue Vaccines ; Feasibility Studies ; HEK293 Cells ; Humans ; Vaccines, Attenuated ; Vaccines, Combined ; Vero Cells
    Chemical Substances Antibodies, Neutralizing ; Antibodies, Viral ; Dengue Vaccines ; Vaccines, Attenuated ; Vaccines, Combined
    Language English
    Publishing date 2022-05-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0267653
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Extended use or reuse of N95 respirators during COVID-19 pandemic: An overview of national regulatory authority recommendations

    Kobayashi, Leticia Mitiko / Marins, Bianca Ramos / Costa, Patrícia Cristina Dos Santos / Perazzo, Hugo / Castro, Rodolfo

    Infect Control Hosp Epidemiol

    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #32319884
    Database COVID19

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  4. Article ; Online: Short communication

    Renata Faria de Carvalho / Lucas de Siqueira Penna Quintaes / Thaís de Cássia de Souza Su / Leticia Mitiko Kobayashi / Ana Cristina Martins de Almeida Nogueira

    PLoS ONE, Vol 17, Iss 5, p e

    Feasibility of dengue vaccine to infect different human cell lines: An alternative potency test using HEK293T cells.

    2022  Volume 0267653

    Abstract: Dengue is caused by an arbovirus that belongs to the Flaviviridae family and there are four distinct, but close related, circulating serotypes. Dengue disease is of great importance for global public health, with vaccination being its main prophylactic ... ...

    Abstract Dengue is caused by an arbovirus that belongs to the Flaviviridae family and there are four distinct, but close related, circulating serotypes. Dengue disease is of great importance for global public health, with vaccination being its main prophylactic measure. However, there is a paucity of biological models for evaluating tetravalent dengue vaccines. The aim of this study was to evaluate the susceptibility of human cell lines HEK293T and THP-1 to a commercial dengue vaccine and test the feasibility of this approach in the development of a potency assay with human cell lines, as a methodological alternative to the golden standard potency assay with VERO cells. In this context, we used a batch of the commercial vaccine Dengvaxia® (CYD-TDV) for the infection tests. We evaluated the presence of the vaccine virus in THP-1 cells, differentiated into macrophages (dTHP-1), and in HEK293T by confocal microscopy, using 4G2 pan-flavivirus antibody. Vaccine infectivity and potency were determined by immunocolorimetric assay using monoclonal antibodies specific for each serotype. The results indicated that the human strain HEK293T was responsive to the tetravalent vaccine, as shown by the presence of virus particles in the cell cytoplasm in a pattern similar to the one observed with VERO cells. Moreover, it was possible to determine the infectivity and potency values of each vaccine virus serotype in the HEK293T, with serotype 4 prevailing over the others. Thus, the human cell line HEK293T provides a potential candidate to be used in assays to determine potency and identity of tetravalent dengue vaccines.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article ; Online: Extended use or reuse of N95 respirators during COVID-19 pandemic

    Kobayashi, Leticia Mitiko / Marins, Bianca Ramos / Costa, Patrícia Cristina dos Santos / Perazzo, Hugo / Castro, Rodolfo

    Infection Control & Hospital Epidemiology

    An overview of national regulatory authority recommendations

    2020  Volume 41, Issue 11, Page(s) 1364–1366

    Keywords Microbiology (medical) ; Epidemiology ; Infectious Diseases ; covid19
    Language English
    Publisher Cambridge University Press (CUP)
    Publishing country uk
    Document type Article ; Online
    ZDB-ID 639378-0
    ISSN 1559-6834 ; 0195-9417 ; 0899-823X
    ISSN (online) 1559-6834
    ISSN 0195-9417 ; 0899-823X
    DOI 10.1017/ice.2020.173
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1714: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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