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  1. Book: Bacterial pathogenesis

    Nordenfelt, Pontus / Collin, Mattias

    methods and protocols

    (Methods in molecular biology ; 2674 ; Springer protocols)

    2023  

    Author's details edited by Pontus Nordenfelt, Mattias Collin
    Series title Methods in molecular biology ; 2674
    Springer protocols
    Collection
    Keywords Bacteria / pathogenicity ; Bacteriological Techniques ; Bacteria / genetics ; Bacteria / immunology ; Host-Pathogen Interactions ; antibody-mediated diseases ; escherichia coli ; immunoglobulin active enzymes ; listeria monocytogenes ; salmonella typhimurium ; staphylococcus aureus ; streptococcus pneumoniae ; streptococcus pyogenes ; subcellular localization of bacterial proteins ; zebrafish
    Language English
    Size xiv, 337 Seiten, Illustrationen, Diagramme, 25.4 cm x 17.8 cm, 0 g
    Edition Second edition
    Publisher Humana Press
    Publishing place New York, NY
    Publishing country United States
    Document type Book
    HBZ-ID HT030043641
    ISBN 978-1-0716-3242-0 ; 9781071632437 ; 1-0716-3242-6 ; 1071632434
    Database Catalogue ZB MED Medicine, Health

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  2. Book: Bacterial pathogenesis

    Nordenfelt, Pontus / Collin, Mattias

    methods and protocols

    (Methods in molecular biology ; 1535 ; Springer protocols ; Life sciences)

    2017  

    Author's details edited by Pontus Nordenfelt, Mattias Collin
    Series title Methods in molecular biology ; 1535
    Springer protocols
    Life sciences
    Collection
    Keywords antibody-mediated diseases ; escherichia coli ; immunoglobulin active enzymes ; listeria monocytogenes ; salmonella typhimurium ; staphylococcus aureus ; streptococcus pneumoniae ; streptococcus pyogenes ; subcellular localization of bacterial proteins ; zebrafish
    Language English
    Size xi, 357 Seiten, Illustrationen, Diagramme, 25.4 cm x 17.8 cm, 0 g
    Publisher Humana Press
    Publishing place New York
    Publishing country United States
    Document type Book
    HBZ-ID HT019199154
    ISBN 978-1-4939-6671-4 ; 1-4939-6671-5 ; 9781493966738 ; 1493966731
    Database Catalogue ZB MED Medicine, Health

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  3. Article: Immunomodulating Enzymes from

    Happonen, Lotta / Collin, Mattias

    Microorganisms

    2024  Volume 12, Issue 1

    Abstract: Streptococcus ... ...

    Abstract Streptococcus pyogenes
    Language English
    Publishing date 2024-01-18
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms12010200
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Book ; Online: Bacterial sensing and signaling

    Collin, Mattias

    one of the keys to the development of novel anti-infective strategies

    2009  

    Author's details vol. ed.: Mattias Collin
    Language English
    Size VIII + 230 S.
    Publisher Karger
    Publishing place Basel
    Publishing country Switzerland
    Document type Book ; Online
    HBZ-ID TT050388041
    ISBN 978-3-8055-9133-1 ; 3-8055-9133-0
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  5. Book: Bacterial sensing and signaling

    Collin, Mattias

    3 tables

    (Contributions to microbiology ; 16)

    2009  

    Author's details vol. ed. Mattias Collin
    Series title Contributions to microbiology ; 16
    Collection
    Keywords Bacteria / chemistry ; Bacterial Processes ; Quorum Sensing / physiology ; Signal Transduction / physiology ; Bakterien ; Chemische Kommunikation ; Signaltransduktion
    Subject Signalübertragung ; Signalvermittlung ; Bacteriophyta ; Bazillen ; Schizomycetes ; Spaltpilze ; Bakterienflora ; Bacterium ; Bacteria ; Eubakterien ; Eubacteriales ; Eubacteria
    Language English
    Size VIII, 230 S. : Ill.
    Publisher Karger
    Publishing place Basel u.a.
    Publishing country Switzerland
    Document type Book
    HBZ-ID HT015975264
    ISBN 3-8055-9132-2 ; 978-3-8055-9132-4 ; 9783805591331 ; 3805591330
    Database Catalogue ZB MED Medicine, Health

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  6. Article ; Online: Antibody glycosylation as an immunological key in health and disease.

    Collin, Mattias

    Glycobiology

    2020  Volume 30, Issue 4, Page(s) 200–201

    MeSH term(s) Animals ; Antibodies/chemistry ; Antibodies/immunology ; Communicable Diseases/immunology ; Glycosylation ; Humans ; Polysaccharides/chemistry ; Polysaccharides/immunology
    Chemical Substances Antibodies ; Polysaccharides
    Language English
    Publishing date 2020-03-26
    Publishing country England
    Document type Introductory Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1067689-2
    ISSN 1460-2423 ; 0959-6658
    ISSN (online) 1460-2423
    ISSN 0959-6658
    DOI 10.1093/glycob/cwaa017
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Book ; Thesis: Streptococcus pyogenes secreted enzymes interacting with the human host

    Collin, Mattias

    2001  

    Author's details Mattias Collin
    Language English ; Swedish
    Size Getr. Zählung : Ill., graph. Darst.
    Publishing country Sweden
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Lund, Univ., Diss., 2001
    Note Zsfassung in schwed. Sprache
    HBZ-ID HT013355222
    ISBN 91-628-4966-2 ; 978-91-628-4966-5
    Database Catalogue ZB MED Medicine, Health

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  8. Article ; Online: Antibody Glycosylation Predicts Relapse in Autoimmune Vasculitis.

    Collin, Mattias

    EBioMedicine

    2017  Volume 17, Page(s) 15

    Language English
    Publishing date 2017-03
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2851331-9
    ISSN 2352-3964
    ISSN (online) 2352-3964
    DOI 10.1016/j.ebiom.2017.02.008
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Antibody Glycosylation Predicts Relapse in Autoimmune Vasculitis

    Mattias Collin

    EBioMedicine, Vol 17, Iss C, p

    2017  Volume 15

    Keywords Medicine ; R
    Language English
    Publishing date 2017-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Expression of the Bacterial Enzyme IdeS Using a GFP Fusion in the Yeast Saccharomyces cerevisiae.

    Lindh, Tova / Collin, Mattias / Lood, Rolf / Carlquist, Magnus

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2674, Page(s) 131–146

    Abstract: Bacterial proteases are important enzymes used in several technical applications where controlled cleavage of proteins is needed. They are challenging enzymes to express recombinantly as parts of the proteome can be hydrolyzed by their activity. The ... ...

    Abstract Bacterial proteases are important enzymes used in several technical applications where controlled cleavage of proteins is needed. They are challenging enzymes to express recombinantly as parts of the proteome can be hydrolyzed by their activity. The eukaryotic model organism Saccharomyces cerevisiae is potentially a good expression host as it tolerates several stress conditions and is known to better express insoluble proteins compared to bacterial systems. In this chapter we describe how the protease IdeS from Streptococcus pyogenes can be expressed in S. cerevisiae. The expression of IdeS was followed by constructing a fused protein with GFP and measuring the fluorescence with flow cytometry. The protease presence was confirmed with a Western blot assay and activity was measured with an in vitro assay. To reduce potentially toxic effect on the host cell, the growth and production phases were separated by using the inducible promoter GAL1p to control recombinant gene expression. The protocol provided may be adopted for other bacterial proteases through minor modifications of the fused protein.
    MeSH term(s) Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Bacterial Proteins/metabolism ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/metabolism ; Fluorescence ; Peptide Hydrolases/metabolism
    Chemical Substances Bacterial Proteins ; Recombinant Fusion Proteins ; Peptide Hydrolases (EC 3.4.-)
    Language English
    Publishing date 2023-05-31
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3243-7_9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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