LIVIVO - Search results -

Search results

Result 1 - 10 of total 69

Search options

Article ; Online: In Vivo and Ex Vivo View of Newt Lens Regeneration.

Tsissios, Georgios / Sallese, Anthony / Chen, Weihao / Miller, Alyssa / Wang, Hui / Del Rio-Tsonis, Katia

Methods in molecular biology (Clifton, N.J.)

2022 Volume 2562, Page(s) 197–208

Abstract: Lens regeneration in the adult newt illustrates a unique example of naturally occurring cell transdifferentiation. During this process, iris pigmented epithelial cells (iPECs) reprogram into a lens, a tissue that is derived from a different embryonic ... ...

Abstract	Lens regeneration in the adult newt illustrates a unique example of naturally occurring cell transdifferentiation. During this process, iris pigmented epithelial cells (iPECs) reprogram into a lens, a tissue that is derived from a different embryonic source. Several methodologies both in vivo and in culture have been utilized over the years to observe this phenomenon. Most recently, Optical Coherence Tomography (OCT) has been identified as an effective tool to study the lens regeneration process in continuity through noninvasive, real-time imaging of the same animal. Described in this chapter are three different methodologies that can be used to observe the newt lens regeneration process both in vivo and ex vivo.
MeSH term(s)	Animals ; Lens, Crystalline ; Salamandridae ; Cell Transdifferentiation ; Epithelial Cells
Language	English
Publishing date	2022-10-21
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-2659-7_13
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article ; Online: Isolation and Characterization of Peritoneal Macrophages from Salamanders.

Sallese, Anthony / Tsissios, Georgios / Pérez-Estrada, J Raúl / Martinez, Arielle / Del Rio-Tsonis, Katia

Methods in molecular biology (Clifton, N.J.)

2022 Volume 2562, Page(s) 259–270

Abstract: Salamanders have been used as research models for centuries. While they exhibit a wide range of biological features not seen in mammals, none has captivated scientists like their ability to regenerate. Interestingly, axolotl macrophages have emerged as ... ...

Abstract	Salamanders have been used as research models for centuries. While they exhibit a wide range of biological features not seen in mammals, none has captivated scientists like their ability to regenerate. Interestingly, axolotl macrophages have emerged as an essential cell population for tissue regeneration. Whether the same is true in other salamanders such as newt species Notophthalmus viridescens, Cynops pyrrhogaster, or Pleurodeles waltl remains to be seen. Unfortunately, regardless of the species, molecular tools to study macrophage function in salamanders are lacking. We propose that the readily available, terminally differentiated peritoneal macrophages from newts or axolotls could be used to validate molecular reagents in the study of macrophage function during tissue regeneration in salamanders.
MeSH term(s)	Animals ; Urodela ; Macrophages, Peritoneal ; Pleurodeles ; Mammals ; Salamandridae
Language	English
Publishing date	2022-10-19
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-2659-7_18
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

Article: DISTINCT METABOLIC STATES DIRECT RETINAL PIGMENT EPITHELIUM CELL FATE DECISIONS.

Perez-Estrada, J Raúl / Tangeman, Jared A / Proto-Newton, Maeve / Sanaka, Harshavardhan / Smucker, Byran / Del Rio-Tsonis, Katia

bioRxiv : the preprint server for biology

2023

Abstract: During tissue regeneration, proliferation, dedifferentiation, and reprogramming are necessary to restore lost structures. However, it is not fully understood how metabolism intersects with these processes. Chicken embryos can regenerate their retina ... ...

Abstract	During tissue regeneration, proliferation, dedifferentiation, and reprogramming are necessary to restore lost structures. However, it is not fully understood how metabolism intersects with these processes. Chicken embryos can regenerate their retina through retinal pigment epithelium (RPE) reprogramming when treated with fibroblast factor 2 (FGF2). Using transcriptome profiling, we uncovered extensive regulation of gene sets pertaining to proliferation, neurogenesis, and glycolysis throughout RPE-to-neural retina reprogramming. By manipulating cell media composition, we determined that glucose, glutamine, or pyruvate are sufficient to support RPE reprogramming identifying glycolysis as a requisite. Conversely, the induction of oxidative metabolism by activation of pyruvate dehydrogenase induces Epithelial-to-mesenchymal transition (EMT), while simultaneously blocking the activation of neural retina fate. We also identify that EMT is partially driven by an oxidative environment. Our findings provide evidence that metabolism controls RPE cell fate decisions and provide insights into the metabolic state of RPE cells, which are prone to fate changes in regeneration and pathologies, such as proliferative vitreoretinopathy.
Language	English
Publishing date	2023-09-27
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.09.26.559631
Database	MEDical Literature Analysis and Retrieval System OnLINE

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: Correction to: An application of slow feature analysis to the genetic sequences of coronaviruses and influenza viruses.

Tsonis, Anastasios A / Wang, Geli / Zhang, Lvyi / Lu, Wenxu / Kayafas, Aristotle / Del Rio-Tsonis, Katia

Human genomics

2021 Volume 15, Issue 1, Page(s) 31

Language	English
Publishing date	2021-06-02
Publishing country	England
Document type	Published Erratum
ZDB-ID	2147618-4
ISSN	1479-7364 ; 1479-7364
ISSN (online)	1479-7364
ISSN	1479-7364
DOI	10.1186/s40246-021-00334-3
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Integrated single-cell multiomics uncovers foundational regulatory mechanisms of lens development and pathology.

Tangeman, Jared A / Rebull, Sofia M / Grajales-Esquivel, Erika / Weaver, Jacob M / Bendezu-Sayas, Stacy / Robinson, Michael L / Lachke, Salil A / Del Rio-Tsonis, Katia

Development (Cambridge, England)

2024 Volume 151, Issue 1

Abstract: Ocular lens development entails epithelial to fiber cell differentiation, defects in which cause congenital cataracts. We report the first single-cell multiomic atlas of lens development, leveraging snRNA-seq, snATAC-seq and CUT&RUN-seq to discover ... ...

Abstract	Ocular lens development entails epithelial to fiber cell differentiation, defects in which cause congenital cataracts. We report the first single-cell multiomic atlas of lens development, leveraging snRNA-seq, snATAC-seq and CUT&RUN-seq to discover previously unreported mechanisms of cell fate determination and cataract-linked regulatory networks. A comprehensive profile of cis- and trans-regulatory interactions, including for the cataract-linked transcription factor MAF, is established across a temporal trajectory of fiber cell differentiation. Furthermore, we identify an epigenetic paradigm of cellular differentiation, defined by progressive loss of the H3K27 methylation writer Polycomb repressive complex 2 (PRC2). PRC2 localizes to heterochromatin domains across master-regulator transcription factor gene bodies, suggesting it safeguards epithelial cell fate. Moreover, we demonstrate that FGF hyper-stimulation in vivo leads to MAF network activation and the emergence of novel lens cell states. Collectively, these data depict a comprehensive portrait of lens fiber cell differentiation, while defining regulatory effectors of cell identity and cataract formation.
MeSH term(s)	Humans ; Multiomics ; Cataract/genetics ; Cell Differentiation/genetics ; Eye ; Lens, Crystalline
Language	English
Publishing date	2024-01-05
Publishing country	England
Document type	Journal Article
ZDB-ID	90607-4
ISSN	1477-9129 ; 0950-1991
ISSN (online)	1477-9129
ISSN	0950-1991
DOI	10.1242/dev.202249
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Ub I Zs.183: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MG 91: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Design and Characterization of Biomimetic Kerateine Aerogel-Electrospun Polycaprolactone Scaffolds for Retinal Cell Culture.

Zeng, Ziqian / Lam, Phuong T / Robinson, Michael L / Del Rio-Tsonis, Katia / Saul, Justin M

Annals of biomedical engineering

2021 Volume 49, Issue 7, Page(s) 1633–1644

Abstract: Age-related macular degeneration (AMD) is a retinal disease that affects 196 million people and causes nearly 9% of blindness worldwide. While several pharmacological approaches slow the effects of AMD, in our opinion, cell-based strategies offer the ... ...

Abstract	Age-related macular degeneration (AMD) is a retinal disease that affects 196 million people and causes nearly 9% of blindness worldwide. While several pharmacological approaches slow the effects of AMD, in our opinion, cell-based strategies offer the most likely path to a cure. We describe the design and initial characterization of a kerateine (obtained by reductive extraction from keratin proteins) aerogel-electrospun polycaprolactone fiber scaffold system. The scaffolds mimic key features of the choroid and the Bruch's membrane, which is the basement membrane to which the cells of the retinal pigment epithelium (RPE) attach. The scaffolds had elastic moduli of 2-7.2 MPa, a similar range as native choroid and Bruch's membrane. ARPE-19 cells attached to the polycaprolactone fibers, remained viable for one week, and proliferated to form a monolayer reminiscent of that needed for retinal repair. These constructs could serve as a model system for testing cell and/or drug treatment strategies or directing ex vivo retinal tissue formation in the treatment of AMD.
MeSH term(s)	Biomimetic Materials/chemistry ; Cell Culture Techniques ; Cell Line ; Humans ; Keratins/chemistry ; Polyesters/chemistry ; Retinal Pigment Epithelium/metabolism ; Tissue Scaffolds/chemistry
Chemical Substances	Polyesters ; polycaprolactone (24980-41-4) ; Keratins (68238-35-7)
Language	English
Publishing date	2021-04-06
Publishing country	United States
Document type	Journal Article
ZDB-ID	185984-5
ISSN	1573-9686 ; 0191-5649 ; 0090-6964
ISSN (online)	1573-9686
ISSN	0191-5649 ; 0090-6964
DOI	10.1007/s10439-021-02756-5
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1018: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Integrated single-cell multiomics uncovers foundational regulatory mechanisms of lens development and pathology.

Tangeman, Jared A / Rebull, Sofia M / Grajales-Esquivel, Erika / Weaver, Jacob M / Bendezu-Sayas, Stacy / Robinson, Michael L / Lachke, Salil A / Rio-Tsonis, Katia Del

bioRxiv : the preprint server for biology

2023

Abstract: Ocular lens development entails epithelial to fiber cell differentiation, defects in which cause congenital cataract. We report the first single-cell multiomic atlas of lens development, leveraging snRNA-seq, snATAC-seq, and CUT&RUN-seq to discover novel ...

Abstract	Ocular lens development entails epithelial to fiber cell differentiation, defects in which cause congenital cataract. We report the first single-cell multiomic atlas of lens development, leveraging snRNA-seq, snATAC-seq, and CUT&RUN-seq to discover novel mechanisms of cell fate determination and cataract-linked regulatory networks. A comprehensive profile of
Language	English
Publishing date	2023-07-11
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.07.10.548451
Database	MEDical Literature Analysis and Retrieval System OnLINE

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: An application of slow feature analysis to the genetic sequences of coronaviruses and influenza viruses.

Tsonis, Anastasios A / Wang, Geli / Zhang, Lvyi / Lu, Wenxu / Kayafas, Aristotle / Del Rio-Tsonis, Katia

Human genomics

2021 Volume 15, Issue 1, Page(s) 26

Abstract: Background: Mathematical approaches have been for decades used to probe the structure of DNA sequences. This has led to the development of Bioinformatics. In this exploratory work, a novel mathematical method is applied to probe the DNA structure of two ...

Abstract	Background: Mathematical approaches have been for decades used to probe the structure of DNA sequences. This has led to the development of Bioinformatics. In this exploratory work, a novel mathematical method is applied to probe the DNA structure of two related viral families: those of coronaviruses and those of influenza viruses. The coronaviruses are SARS-CoV-2, SARS-CoV-1, and MERS. The influenza viruses include H1N1-1918, H1N1-2009, H2N2-1957, and H3N2-1968. Methods: The mathematical method used is the slow feature analysis (SFA), a rather new but promising method to delineate complex structure in DNA sequences. Results: The analysis indicates that the DNA sequences exhibit an elaborate and convoluted structure akin to complex networks. We define a measure of complexity and show that each DNA sequence exhibits a certain degree of complexity within itself, while at the same time there exists complex inter-relationships between the sequences within a family and between the two families. From these relationships, we find evidence, especially for the coronavirus family, that increasing complexity in a sequence is associated with higher transmission rate but with lower mortality. Conclusions: The complexity measure defined here may hold a promise and could become a useful tool in the prediction of transmission and mortality rates in future new viral strains.
MeSH term(s)	Betacoronavirus/classification ; Betacoronavirus/genetics ; Betacoronavirus/physiology ; Coronavirus Infections/mortality ; Coronavirus Infections/transmission ; Coronavirus Infections/virology ; Evolution, Molecular ; Humans ; Influenza A virus/classification ; Influenza A virus/genetics ; Influenza A virus/physiology ; Influenza, Human/mortality ; Influenza, Human/transmission ; Influenza, Human/virology ; Models, Genetic ; Sequence Analysis, DNA ; Species Specificity ; Time Factors
Language	English
Publishing date	2021-05-07
Publishing country	England
Document type	Journal Article
ZDB-ID	2147618-4
ISSN	1479-7364 ; 1479-7364
ISSN (online)	1479-7364
ISSN	1479-7364
DOI	10.1186/s40246-021-00327-2
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Generation of a Retina Reporter hiPSC Line to Label Progenitor, Ganglion, and Photoreceptor Cell Types.

Lam, Phuong T / Gutierrez, Christian / Del Rio-Tsonis, Katia / Robinson, Michael L

Translational vision science & technology

2020 Volume 9, Issue 3, Page(s) 21

Abstract: Purpose: Early in mammalian eye development, : Methods: CRISPR/Cas9-mediated homology-directed repair (HDR) in hiPSCs facilitated the replacement of the : Results: Retinal organoids formed from the PGP1 line expressed appropriate fluorescent ... ...

Abstract	Purpose: Early in mammalian eye development, Methods: CRISPR/Cas9-mediated homology-directed repair (HDR) in hiPSCs facilitated the replacement of the Results: Retinal organoids formed from the PGP1 line expressed appropriate fluorescent proteins consistent with the differentiation of NRPs, RGCs, and PRs. Organoids produced from the PGP1 line expressed transcripts consistent with the development of all major retinal cell types. Conclusions and translational relevance: The PGP1 line offers a powerful new tool to study retinal development, retinal reprogramming, and therapeutic drug screening.
MeSH term(s)	Animals ; Cell Differentiation ; Humans ; Induced Pluripotent Stem Cells ; Organoids ; Photoreceptor Cells ; Retina
Language	English
Publishing date	2020-02-18
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2674602-5
ISSN	2164-2591
ISSN	2164-2591
DOI	10.1167/tvst.9.3.21
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Characterizing the lens regeneration process in Pleurodeles waltl.

Tsissios, Georgios / Theodoroudis-Rapp, Gabriella / Chen, Weihao / Sallese, Anthony / Smucker, Byran / Ernst, Lake / Chen, Junfan / Xu, Yiqi / Ratvasky, Sophia / Wang, Hui / Del Rio-Tsonis, Katia

Differentiation; research in biological diversity

2023 Volume 132, Page(s) 15–23

Abstract: Background: Aging and regeneration are heavily linked processes. While it is generally accepted that regenerative capacity declines with age, some vertebrates, such as newts, can bypass the deleterious effects of aging and successfully regenerate a lens ...

Abstract	Background: Aging and regeneration are heavily linked processes. While it is generally accepted that regenerative capacity declines with age, some vertebrates, such as newts, can bypass the deleterious effects of aging and successfully regenerate a lens throughout their lifetime. Results: Here, we used Spectral-Domain Optical Coherence Tomography (SD-OCT) to monitor the lens regeneration process of larvae, juvenile, and adult newts. While all three life stages were able to regenerate a lens through transdifferentiation of the dorsal iris pigment epithelial cells (iPECs), an age-related change in the kinetics of the regeneration process was observed. Consistent with these findings, iPECs from older animals exhibited a delay in cell cycle re-entry. Furthermore, it was observed that clearance of the extracellular matrix (ECM) was delayed in older organisms. Conclusions: Collectively, our results suggest that although lens regeneration capacity does not decline throughout the lifespan of newts, the intrinsic and extrinsic cellular changes associated with aging alter the kinetics of this process. By understanding how these changes affect lens regeneration in newts, we can gain important insights for restoring the age-related regeneration decline observed in most vertebrates.
MeSH term(s)	Animals ; Pleurodeles ; Salamandridae ; Lens, Crystalline ; Extracellular Matrix ; Cell Division
Language	English
Publishing date	2023-03-11
Publishing country	England
Document type	Journal Article
ZDB-ID	184540-8
ISSN	1432-0436 ; 0301-4681
ISSN (online)	1432-0436
ISSN	0301-4681
DOI	10.1016/j.diff.2023.02.003
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1170: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito