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Article ; Online: Lizhong decoction ameliorates ulcerative colitis by inhibiting ferroptosis of enterocytes via the Nrf2/SLC7A11/GPX4 pathway.

Li, Wenwen / Wang, Yu / Zhang, Yun / Fan, Yuwen / Liu, Jinsong / Zhu, Ke / Jiang, Shu / Duan, Jinao

2024 Volume 326, Page(s) 117966

Abstract: ... effective way to treat many diseases. Lizhong decoction (LZD), a classical prescription composed ...

Abstract	Ethnopharmacology relevance: Traditional herbal medicines have been considered as a novel and effective way to treat many diseases. Lizhong decoction (LZD), a classical prescription composed of Zingiber officinale Rosc., Panax ginseng C. A. Mey., Atractylodes macrocephala Koidz., and Glycyrrhiza uralensis Fisch., has been used to treat gastrointestinal disorders in clinical practices for thousands of years. However, the mechanism of LZD in alleviating ulcerative colitis (UC) is still unclear. Aim of the study: The purpose of this study was to clarify the potential molecular mechanism of LZD in improving UC. Materials and methods: The amelioration of LZD on dextran sodium sulfate (DSS)-induced UC mice was evaluated by body weight, colon length, pathology of colon tissues, pro-inflammatory cytokines, and intestinal tight junction (TJ) proteins. Moreover, the gene expression profiles of UC patients were extracted to investigate potential pathological mechanisms of UC. The influence of LZD on ferroptosis was analyzed by iron load, malondialdehyde (MDA), and the expression of ferroptosis-associated proteins. Meanwhile, the inhibition of LZD on oxidative stress (OS) was assessed by the superoxide dismutase (SOD) activity, as well as the expression levels of glutathione (GSH) and glutathione disulfide (GSSG). Furthermore, the influence of LZD on ferroptosis was assessed by inhibiting nuclear factor (erythroid-derived-2)-like 2 (Nrf2). Results: LZD showed significant therapeutic effects in UC mice, including reduction of intestinal injury and inflammation. Moreover, LZD treatment notably upregulated the expression of TJ proteins. Further investigation indicated that LZD significantly inhibited the ferroptosis of enterocytes by decreasing iron load and MDA, and increasing the expression levels of solute carrier family 7 member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4) in colon tissues. Furthermore, the decreased activity of SOD, reduced level of GSH, and increased content of GSSG in UC mice were notably reversed by LZD. Consistent with in vivo results, LZD could markedly inhibit ferroptosis and OS in RSL3-induced Caco-2 cells. Mechanistically, LZD alleviated ferroptosis by suppressing OS through the activation of Nrf2 signaling. Conclusions: Collectively, LZD remarkably improved intestinal pathological injury in UC mice, and its potential mechanism was the suppression of ferroptosis in enterocytes by the Nrf2/SLC7A11/GPX4 pathway.
MeSH term(s)	Humans ; Animals ; Mice ; Colitis, Ulcerative/chemically induced ; Colitis, Ulcerative/drug therapy ; Enterocytes ; Phospholipid Hydroperoxide Glutathione Peroxidase ; NF-E2-Related Factor 2 ; Glutathione Disulfide ; Caco-2 Cells ; Ferroptosis ; Glutathione ; Iron ; Superoxide Dismutase ; Dextran Sulfate/toxicity ; Mice, Inbred C57BL ; Colitis ; Amino Acid Transport System y+
Chemical Substances	Phospholipid Hydroperoxide Glutathione Peroxidase (EC 1.11.1.12) ; NF-E2-Related Factor 2 ; Glutathione Disulfide (ULW86O013H) ; Glutathione (GAN16C9B8O) ; Iron (E1UOL152H7) ; Superoxide Dismutase (EC 1.15.1.1) ; Dextran Sulfate (9042-14-2) ; SLC7A11 protein, human ; Amino Acid Transport System y+
Language	English
Publishing date	2024-02-22
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2024.117966
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Article ; Online: Investigation of the material basis and mechanism of Lizhong decoction in ameliorating ulcerative colitis based on spectrum-effect relationship and network pharmacology.

Zhang, Yun / Li, Wen-Wen / Wang, Yu / Fan, Yu-Wen / Wang, Qu-Yi / Liu, Chen / Jiang, Shu / Shang, Er-Xin / Duan, Jin-Ao

Journal of ethnopharmacology

2023 Volume 323, Page(s) 117666

Abstract: Ethnopharmacological relevance: Lizhong decoction (LZD), a classical herbal prescription recorded ...

Abstract	Ethnopharmacological relevance: Lizhong decoction (LZD), a classical herbal prescription recorded by Zhang Zhongjing in Treatise on Febrile and Miscellaneous Diseases, has been extensively used to treat ulcerative colitis (UC) in clinical practice for thousands of years. However, its material basis and underlying mechanism are not yet clear. Aim of the study: This study aims to explore the material basis and potential mechanism of LZD against UC based on the spectrum-effect relationship and network pharmacology. Materials and methods: First, LZD was extracted by a systematic solvent extraction method into four parts. Ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) technique was used to identify the compounds from different polar parts, and dextran sulfate sodium (DSS)-induced colitis model was used to evaluate the efficacy of each fraction. Then, the spectrum-effect analyses of compounds and efficacy indicators were established via grey relational analysis (GRA), bivariate correlation analysis (BCA) and partial least squares regression (PLSR). Finally, the potential mechanism of LZD for UC therapy was explored by network pharmacology, and the results were further verified by molecular docking and reverse transcription quantitative polymerase chain reaction (RT-qPCR). Results: 66 chemical components of LZD were identified by UPLC-Q-TOF-MS/MS technology. The pharmacodynamic results showed that extraction parts of LZD had different therapeutic effects on UC, among which ethyl acetate and n-butanol extracts had significant anti-colitis effects, which might be the main effective fractions of LZD. Furthermore, the spectrum-effect analyses indicated that 21 active ingredients such as liquiritin apioside, neolicuroside, formononetin, ginsenoside Rg1, 6-gingesulfonic acid, licoricesaponin A3, liquiritin, glycyrrhizic acid were the main material basis for LZD improving UC. Based on the above results, network pharmacology suggested that the amelioration of LZD on UC might be closely related to the PI3K-Akt signaling pathway. Additionally, molecular docking technology and RT-qPCR further verified that LZD could markedly inhibit the PI3K-Akt signaling pathway. Conclusion: Overall, our study first identified the chemical compositions of LZD by using UPLC-Q-TOF-MS/MS. Furthermore, the material basis and potential mechanism of LZD in improving UC were comprehensively elucidated via spectrum-effect relationships, network pharmacology, molecular docking and experimental verification. The proposed strategy provided a systematic approach for exploring how herbal medicines worked. More importantly, it laid the solid foundation for further clinical application and rational development of LZD.
MeSH term(s)	Humans ; Colitis, Ulcerative/chemically induced ; Colitis, Ulcerative/drug therapy ; Molecular Docking Simulation ; Network Pharmacology ; Phosphatidylinositol 3-Kinases ; Proto-Oncogene Proteins c-akt ; Tandem Mass Spectrometry ; Colitis ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use
Chemical Substances	Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Drugs, Chinese Herbal
Language	English
Publishing date	2023-12-28
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.117666
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Article ; Online: Lizhong decoction ameliorates pulmonary infection secondary to severe traumatic brain injury in rats by regulating the intestinal physical barrier and immune response.

Miao, Yulu / Fan, Xuejin / Wei, Luge / Wang, Bin / Diao, Fengyin / Fu, Jiafeng / Zhuang, Pengwei / Zhang, Yanjun

Journal of ethnopharmacology

2023 Volume 311, Page(s) 116346

Abstract: ... traumatic brain injury (sTBI) is closely related to damage to the intestinal barrier. Lizhong decoction (LZD) is ...

Abstract	Ethnopharmacological relevance: The pathogenesis of pulmonary infection secondary to severe traumatic brain injury (sTBI) is closely related to damage to the intestinal barrier. Lizhong decoction (LZD) is a prominent traditional Chinese medicine (TCM) that is widely used in clinical treatment to regulate gastrointestinal movement and enhance resistance. Nevertheless, the role and mechanism of LZD in lung infection secondary to sTBI have yet to be elucidated. Aim of the study: Here, we evaluate the therapeutic effect of LZD on pulmonary infection secondary to sTBI in rats and discuss potential regulatory mechanisms. Materials and methods: The chemical constituents of LZD were analyzed by ultra-high performance liquid chromatography-Q Exactive-tandem mass spectrometry(UPLC-QE-MS/MS). The efficacy of LZD on rats with lung infection secondary to sTBI was examined by changes in brain morphology, coma time, brain water content, mNSS score, colony counts, 16S rRNA/RNaseP/MRP30 kDa(16S/RPP30), myeloperoxidase (MPO) content and pathology of lung tissue. The concentration of fluorescein isothiocyanate(FITC)-dextran in serum and the contents of secretory immunoglobulin A (SIgA) in colon tissue were detected by enzyme-linked immunosorbent assay (ELISA). Subsequently, Alcian Blue Periodic acid Schiff (AB-PAS) was used to detect colonic goblet cells. Immunofluorescence (IF) was used to detect the expression of tight junction proteins. The proportions of CD3 Results: Twenty-nine chemical constituents of LZD were revealed with UPLC-QE-MS/MS analysis. Administration of LZD significantly reduced colony counts, 16S/RPP30 and MPO content in lung infection secondary to sTBI rats. In addition, LZD also reduced the serum FITC-glucan content and the SIgA content of the colon. Additionally, LZD significantly increased the number of colonic goblet cells and the expression of tight junction proteins. Furthermore, LZD significantly decreased the proportion of CD3 Conclusion: LZD can improves sTBI secondary lung infection by regulating the intestinal physical barrier and immune response. Thees results suggested that LZD may be a prospective treatment for pulmonary infection secondary to sTBI.
MeSH term(s)	Rats ; Animals ; Tandem Mass Spectrometry ; Fluorescein-5-isothiocyanate ; CD8-Positive T-Lymphocytes ; RNA, Ribosomal, 16S ; Brain Injuries, Traumatic ; Pneumonia ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Immunity ; RNA, Messenger ; Tight Junction Proteins
Chemical Substances	Fluorescein-5-isothiocyanate (I223NX31W9) ; RNA, Ribosomal, 16S ; Drugs, Chinese Herbal ; RNA, Messenger ; Tight Junction Proteins
Language	English
Publishing date	2023-03-08
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.116346
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Article: Transcriptomic Analysis of Fuzi Lizhong Decoction for the Treatment of Stomach Ulcers.

Xin, Yang / Wang, Haijun / Xu, Lei

Evidence-based complementary and alternative medicine : eCAM

2020 Volume 2020, Page(s) 3291853

Abstract: This study aims to understand the treatment of stomach ulcers with FLD and to identify its potential target genes as well as related pathways by transcriptomic analysis. Rat stomach mRNA from a blank group (BG), a model group (MG), an untreated-model ... ...

Abstract	This study aims to understand the treatment of stomach ulcers with FLD and to identify its potential target genes as well as related pathways by transcriptomic analysis. Rat stomach mRNA from a blank group (BG), a model group (MG), an untreated-model group (u-MG), and a treated group (TG) was sequenced. A partial least-squares discriminant analysis (PLS-DA) was used to differentiate the MG from the BG, and the Deseq2 R Package was used to identify differentially expressed genes between these groups. Furthermore,
Language	English
Publishing date	2020-02-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	2171158-6
ISSN	1741-4288 ; 1741-427X
ISSN (online)	1741-4288
ISSN	1741-427X
DOI	10.1155/2020/3291853
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Article: Filtration and qualification for target biomarkers of traditional Chinese medicine formula "fuzi lizhong decoction" acting on stomach ulcer by UPLC/Q-TOF MS.

Xin, Yang / Yu, Kaichen / Yu, Yang / Wang, Haijun

Pakistan journal of pharmaceutical sciences

2022 Volume 35, Issue 2, Page(s) 519–528

Abstract: This study aimed to illustrate the curative effect of Fuzi Lizhong Decoction, which is composed ... metabolism. In conclusion, Fuzi Lizhong Decoction may cure stomach ulcer by improving tryptophan metabolism ...

Abstract	This study aimed to illustrate the curative effect of Fuzi Lizhong Decoction, which is composed of Aconitum carmichaeli Debeaux, Zingiber officinale Roscoe, Codonopsis pilosula (Franch.), Atractylodes macrocephala Koidz and Glycyrrhiza uralensis Fisch. and contained components such as benzoyl aconitine, benzoyl aconitine, atractyl lactone I, atractylenolide II, ginsenoside, emodin, glycyrrhizin, glycyrrhizin, 8-gingerol and 10-gingerol, on stomach ulcer based on ultra-performance liquid chromatography/mass spectrometry metabolomics analysis. Rats urine samples of three groups were detected by ultra-performance liquid chromatography/mass spectrometry. Then, stomach ulcer biomarkers were filtered out by EZinfo 2.0 software. After that, analysis of variance was used for picking up significantly retroved biomarkers in treated group. Compounds were identified based on MS/MS spectrum, accurate mass weight and retention time. Twelve metabolites including 2-Methylhippuic acid, Kynurenic acid, 2-Indolecarboxylic acid, Pyrocatechol, Caproic acid, 2,3-Dihydrobenzofuran, 4-Ethylphenol, Lanthionine ketimine, Traumatic acid, Indole-3-carboxilic acid-O-sulphate, Vanillic acid 4-sulfate, 6-Mecaptopurine ribonudeoside 5'-diphosphate were identified as stomach ulcer biomarkers. Among them, ten ones except for 2-Methylhippuic acid and 2-Indolecarboxylic acid retrieved. Pathways involved tryptophan metabolism, benzoate degradation, bisphenol degradation and α-linolenic acid metabolism. In conclusion, Fuzi Lizhong Decoction may cure stomach ulcer by improving tryptophan metabolism, benzoate degradation, bisphenol degradation and α-linolenic acid metabolism.
MeSH term(s)	Aconitine ; Animals ; Benzoates ; Biomarkers ; Chromatography, High Pressure Liquid/methods ; Diterpenes ; Drugs, Chinese Herbal ; Glycyrrhizic Acid ; Medicine, Chinese Traditional ; Rats ; Stomach Ulcer ; Tandem Mass Spectrometry/methods ; Tryptophan ; alpha-Linolenic Acid
Chemical Substances	Benzoates ; Biomarkers ; Diterpenes ; Drugs, Chinese Herbal ; fuzi drug herbal ; alpha-Linolenic Acid (0RBV727H71) ; Glycyrrhizic Acid (6FO62043WK) ; Tryptophan (8DUH1N11BX) ; Aconitine (X8YN71D5WC)
Language	English
Publishing date	2022-06-01
Publishing country	Pakistan
Document type	Journal Article
ZDB-ID	885131-1
ISSN	1011-601X
ISSN	1011-601X
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 6588: Show issues

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Article: Action Mechanism Underlying Improvement Effect of Fuzi Lizhong Decoction on Nonalcoholic Fatty Liver Disease: A Study Based on Network Pharmacology and Molecular Docking.

Luo, Zheng / Xia, Lu-Yun / Tang, Yu-Qin / Huang, Lili / Liu, Dan / Huai, Wen-Ying / Zhang, Chun-Jiang / Wang, Yan-Qiu / Xie, Yong-Mei / Yin, Qiao-Zhi / Chen, Yun-Hui / Zhang, Tian-E

Evidence-based complementary and alternative medicine : eCAM

2022 Volume 2022, Page(s) 1670014

Abstract: ... of traditional Chinese medicine (TCM) formula Fuzi Lizhong Decoction (FLD) for nonalcoholic fatty liver disease (NAFLD) treatment ...

Abstract	Objective: This study aimed to decipher the bioactive compounds and potential mechanism of traditional Chinese medicine (TCM) formula Fuzi Lizhong Decoction (FLD) for nonalcoholic fatty liver disease (NAFLD) treatment via an integrative network pharmacology approach. Methods: The candidate compounds of FLD and its relative targets were obtained from the TCMSP and PharmMapper web server, and the intersection genes for NAFLD were discerned using OMIM, GeneCards, and DisGeNET. Then, the PPI and component-target-pathway networks were constructed. Moreover, GO enrichment and KEGG pathway analysis were performed to investigate the potential signaling pathways associated with FLD's effect on NAFLD. Eventually, molecular docking simulation was carried out to validate the binding affinity between potential core components and key targets. Results: A total of 143 candidate active compounds and 129 relative drug targets were obtained, in which 61 targets were overlapped with NAFLD. The PPI network analysis identified ALB, MAPK1, CASP3, MARK8, and AR as key targets, mainly focusing on cellular response to organic cyclic compound, steroid metabolic process, and response to steroid hormone in the biological processes. The KEGG pathway analysis demonstrated that 16 signaling pathways were closely correlated with FLD's effect on NALFD with cancer pathways, Th17 cell differentiation, and IL-17 signaling pathways as the most significant ones. In addition, the molecular docking analysis revealed that the core active compounds of FLD, such as 3'-methoxyglabridin, chrysanthemaxanthin, and Gancaonin H, had a high binding activity with such key targets as ALB, MAPK1, and CASP3. Conclusions: This study suggested that FLD exerted its effect on NAFLD via modulating multitargets with multicompounds through multipathways. It also demonstrated that the network pharmacology-based approach might provide insights for understanding the interrelationship between complex diseases and interventions of the TCM formula.
Language	English
Publishing date	2022-01-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	2171158-6
ISSN	1741-4288 ; 1741-427X
ISSN (online)	1741-4288
ISSN	1741-427X
DOI	10.1155/2022/1670014
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Article: Lizhong Decoction Ameliorates Ulcerative Colitis in Mice via Regulation of Plasma and Urine Metabolic Profiling.

Wang, Ling / Tao, Jin-Hua / Chen, Yi-Fan / Shen, Yu-Meng / Jiang, Shu

Chinese journal of integrative medicine

2021 Volume 28, Issue 11, Page(s) 1015–1022

Abstract: Objective: To elucidate the mechanism of Lizhong Decoction (LZD) in treating dextran ...

Abstract	Objective: To elucidate the mechanism of Lizhong Decoction (LZD) in treating dextran sodium sulfate (DSS)-induced colitis in mice based on metabonomics. Methods: Thirty-six mice were randomly divided into 6 groups, including normal, model, low- (1.365 g/kg), medium- (4.095 g/kg) and high dose (12.285 g/kg) LZD and salazosulfadimidine (SASP) groups, 6 mice in each group. Colitis model mice were induced by DSS admistration for 7 days, and treated with low, medium and high dose LZD extract and positive drug SASP. Metabolic comparison of DSS-induced colitis and normal mice was investigated by using ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass (UPLC-Q-TOF/MS) combined with Metabolynx™ software. Results: The metabolic profiles of plasma and urine in colitis mice were distinctly ameliorated after LZD treatment (P<0.05). Potential biomarkers (9 in serum and 4 in urine) were screened and tentatively identified. The endogenous metabolites were mainly involved in primary bile acid, sphingolipid, linoleic acid, arachidonic acid, amino acids (alanine, aspartate, and glutamate), butanoate and glycerophospholipid metabolism in plasma, and terpenoid backbone biosynthesis, glycerophospholipid and tryptophan metabolism in urine. After LZD treatment, these markers notably restored to normal levels. Conclusions: The study revealed the underlying mechanism of LZD on amelioration of ulcerative colitis based on metabonomics, which laid a foundation for further exploring the pathological and physiological mechanism, early diagnosis, and corresponding drug development of colitis.
MeSH term(s)	Mice ; Animals ; Colitis, Ulcerative/chemically induced ; Colitis, Ulcerative/drug therapy ; Tryptophan/adverse effects ; Aspartic Acid ; Dextrans/adverse effects ; Drugs, Chinese Herbal/adverse effects ; Colitis/chemically induced ; Colitis/drug therapy ; Biomarkers/metabolism ; Amino Acids/adverse effects ; Glycerophospholipids/therapeutic use ; Sphingolipids/adverse effects ; Bile Acids and Salts/adverse effects ; Glutamates/adverse effects ; Alanine/adverse effects ; Arachidonic Acids/adverse effects ; Linoleic Acids/adverse effects ; Terpenes
Chemical Substances	Tryptophan (8DUH1N11BX) ; Aspartic Acid (30KYC7MIAI) ; Dextrans ; Drugs, Chinese Herbal ; Biomarkers ; Amino Acids ; Glycerophospholipids ; Sphingolipids ; Bile Acids and Salts ; Glutamates ; Alanine (OF5P57N2ZX) ; Arachidonic Acids ; Linoleic Acids ; Terpenes
Language	English
Publishing date	2021-09-29
Publishing country	China
Document type	Journal Article
ZDB-ID	2171254-2
ISSN	1993-0402 ; 1672-0415
ISSN (online)	1993-0402
ISSN	1672-0415
DOI	10.1007/s11655-021-3299-4
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Zs.A 5823: Show issues

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Article ; Online: Lizhong decoction ameliorates pulmonary infection secondary to severe traumatic brain injury in rats by regulating the intestinal physical barrier and immune response

Miao, Yulu / Fan, Xuejin / Wei, Luge / Wang, Bin / Diao, Fengyin / Fu, Jiafeng / Zhuang, Pengwei / Zhang, Yanjun

Journal of Ethnopharmacology. 2023 July, v. 311 p.116346-

2023

Abstract: ... closely related to damage to the intestinal barrier. Lizhong decoction (LZD) is a prominent ...

Abstract	The pathogenesis of pulmonary infection secondary to severe traumatic brain injury (sTBI) is closely related to damage to the intestinal barrier. Lizhong decoction (LZD) is a prominent traditional Chinese medicine (TCM) that is widely used in clinical treatment to regulate gastrointestinal movement and enhance resistance. Nevertheless, the role and mechanism of LZD in lung infection secondary to sTBI have yet to be elucidated. Here, we evaluate the therapeutic effect of LZD on pulmonary infection secondary to sTBI in rats and discuss potential regulatory mechanisms. The chemical constituents of LZD were analyzed by ultra-high performance liquid chromatography-Q Exactive-tandem mass spectrometry(UPLC-QE-MS/MS). The efficacy of LZD on rats with lung infection secondary to sTBI was examined by changes in brain morphology, coma time, brain water content, mNSS score, colony counts, 16S rRNA/RNaseP/MRP30 kDa(16S/RPP30), myeloperoxidase (MPO) content and pathology of lung tissue. The concentration of fluorescein isothiocyanate(FITC)-dextran in serum and the contents of secretory immunoglobulin A (SIgA) in colon tissue were detected by enzyme-linked immunosorbent assay (ELISA). Subsequently, Alcian Blue Periodic acid Schiff (AB-PAS) was used to detect colonic goblet cells. Immunofluorescence (IF) was used to detect the expression of tight junction proteins. The proportions of CD3⁺ cell, CD4⁺CD8⁺ T cells, CD45⁺ cell and CD103+ cells in the colon were analyzed by flow cytometry (FC). In addition, colon transcriptomics were analyzed by Illumina mRNA-Seq sequencing. Real-time quantitative polymerase chain reaction (qRT‒PCR) was used to verify the genes associated with LZD alleviation of intestinal barrier function. Twenty-nine chemical constituents of LZD were revealed with UPLC-QE-MS/MS analysis. Administration of LZD significantly reduced colony counts, 16S/RPP30 and MPO content in lung infection secondary to sTBI rats. In addition, LZD also reduced the serum FITC-glucan content and the SIgA content of the colon. Additionally, LZD significantly increased the number of colonic goblet cells and the expression of tight junction proteins. Furthermore, LZD significantly decreased the proportion of CD3⁺ cell, CD4⁺CD8⁺ T cells,CD45+ and CD103+ cells in colon tissue. Transcriptomic analysis identified 22 upregulated genes and 56 downregulated genes in sTBI compared to the sham group. The levels of seven genes were recovered after LZD treatment. qRT‒PCR successfully validated two genes (Jchain and IL-6) at the mRNA level. LZD can improves sTBI secondary lung infection by regulating the intestinal physical barrier and immune response. Thees results suggested that LZD may be a prospective treatment for pulmonary infection secondary to sTBI.
Keywords	Oriental traditional medicine ; blood serum ; brain ; brain damage ; colon ; coma ; enzyme-linked immunosorbent assay ; flow cytometry ; fluorescein ; fluorescent antibody technique ; gene expression ; immune response ; immunoglobulin A ; interleukin-6 ; isothiocyanates ; lungs ; mass spectrometry ; myeloperoxidase ; pathogenesis ; quantitative polymerase chain reaction ; therapeutics ; tight junctions ; transcriptomics ; water content ; Lizhong decoction ; Lung infection secondary to sTBI ; Intestinal barrier ; Tight junction
Language	English
Dates of publication	2023-07
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2023.116346
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Action Mechanism Underlying Improvement Effect of Fuzi Lizhong Decoction on Nonalcoholic Fatty Liver Disease

Zheng Luo / Lu-Yun Xia / Yu-Qin Tang / Lili Huang / Dan Liu / Wen-Ying Huai / Chun-Jiang Zhang / Yan-Qiu Wang / Yong-Mei Xie / Qiao-Zhi Yin / Yun-Hui Chen / Tian-E. Zhang

Evidence-Based Complementary and Alternative Medicine, Vol

A Study Based on Network Pharmacology and Molecular Docking

2022 Volume 2022

Abstract: ... of traditional Chinese medicine (TCM) formula Fuzi Lizhong Decoction (FLD) for nonalcoholic fatty liver disease (NAFLD) treatment ...

Abstract	Objective. This study aimed to decipher the bioactive compounds and potential mechanism of traditional Chinese medicine (TCM) formula Fuzi Lizhong Decoction (FLD) for nonalcoholic fatty liver disease (NAFLD) treatment via an integrative network pharmacology approach. Methods. The candidate compounds of FLD and its relative targets were obtained from the TCMSP and PharmMapper web server, and the intersection genes for NAFLD were discerned using OMIM, GeneCards, and DisGeNET. Then, the PPI and component-target-pathway networks were constructed. Moreover, GO enrichment and KEGG pathway analysis were performed to investigate the potential signaling pathways associated with FLD’s effect on NAFLD. Eventually, molecular docking simulation was carried out to validate the binding affinity between potential core components and key targets. Results. A total of 143 candidate active compounds and 129 relative drug targets were obtained, in which 61 targets were overlapped with NAFLD. The PPI network analysis identified ALB, MAPK1, CASP3, MARK8, and AR as key targets, mainly focusing on cellular response to organic cyclic compound, steroid metabolic process, and response to steroid hormone in the biological processes. The KEGG pathway analysis demonstrated that 16 signaling pathways were closely correlated with FLD’s effect on NALFD with cancer pathways, Th17 cell differentiation, and IL-17 signaling pathways as the most significant ones. In addition, the molecular docking analysis revealed that the core active compounds of FLD, such as 3′-methoxyglabridin, chrysanthemaxanthin, and Gancaonin H, had a high binding activity with such key targets as ALB, MAPK1, and CASP3. Conclusions. This study suggested that FLD exerted its effect on NAFLD via modulating multitargets with multicompounds through multipathways. It also demonstrated that the network pharmacology-based approach might provide insights for understanding the interrelationship between complex diseases and interventions of the TCM formula.
Keywords	Other systems of medicine ; RZ201-999
Subject code	540
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Transcriptomic Analysis of Fuzi Lizhong Decoction for the Treatment of Stomach Ulcers

Yang Xin / Haijun Wang / Lei Xu

Evidence-Based Complementary and Alternative Medicine, Vol

2020 Volume 2020

Abstract	This study aims to understand the treatment of stomach ulcers with FLD and to identify its potential target genes as well as related pathways by transcriptomic analysis. Rat stomach mRNA from a blank group (BG), a model group (MG), an untreated-model group (u-MG), and a treated group (TG) was sequenced. A partial least-squares discriminant analysis (PLS-DA) was used to differentiate the MG from the BG, and the Deseq2 R Package was used to identify differentially expressed genes between these groups. Furthermore, t-tests based on transcripts per million (TPM) were used to select different genes between MG and BG and significantly retrieved genes in TG, except for self-retrieved genes in u-MG. Finally, pathways regulated by retrieved genes were analyzed with KEGG database. Results showed that 327 of the 32,623 total detected genes were different (p<0.05) between the MG and BG. Among these genes, eighteen genes were significantly retrieved after rats were treated with FLD in TG, and they were considered as target genes on which FLD acted. In conclusion, FLD was deduced to cure stomach ulcers by affecting glycerolipid metabolism, fatty acid degradation, circadian entrainment, circadian rhythm, and dopaminergic synapse.
Keywords	Other systems of medicine ; RZ201-999
Subject code	572
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Hindawi Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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