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  1. Article: Improvement of native structure-based peptides as efficient inhibitors of protein-protein interactions of SARS-CoV-2 spike protein and human ACE2.

    Odolczyk, Norbert / Klim, Joanna / Podsiadła-Białoskórska, Małgorzata / Winiewska-Szajewska, Maria / Szolajska, Ewa / Zielenkiewicz, Urszula / Poznański, Jarosław / Zielenkiewicz, Piotr

    Frontiers in molecular biosciences

    2022  Volume 9, Page(s) 983014

    Abstract: New pathogens responsible for novel human disease outbreaks in the last two decades are mainly the respiratory system viruses. Not different was the last pandemic episode, caused by infection of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV- ...

    Abstract New pathogens responsible for novel human disease outbreaks in the last two decades are mainly the respiratory system viruses. Not different was the last pandemic episode, caused by infection of a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). One of the extensively explored targets, in the recent scientific literature, as a possible way for rapid development of COVID-19 specific drug(s) is the interaction between the receptor-binding domain of the virus' spike (S) glycoprotein and human receptor angiotensin-converting enzyme 2 (
    Language English
    Publishing date 2022-09-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2814330-9
    ISSN 2296-889X
    ISSN 2296-889X
    DOI 10.3389/fmolb.2022.983014
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  2. Article ; Online: A possible role of the ecdysone receptor in modulating gonad-inhibiting hormone gene expression in the black tiger prawn, Penaeus monodon

    Kluebsoongnoen, Jakkapong / Saensuwanna, Apisara / Jozghorbani, Maryam / Ho, Teerapong / Szolajska, Ewa / Sarnowski, Tomasz J. / Udomkit, Apinunt

    Aquaculture. 2023 May, v. 569 p.739393-

    2023  

    Abstract: Gonad-inhibiting hormone (GIH) is a neuronal eyestalk peptide that inhibits ovarian maturation in crustaceans. In this study, the aim was to investigate the transcriptional regulation of the GIH gene in the black tiger prawn (Penaeus monodon). An ... ...

    Abstract Gonad-inhibiting hormone (GIH) is a neuronal eyestalk peptide that inhibits ovarian maturation in crustaceans. In this study, the aim was to investigate the transcriptional regulation of the GIH gene in the black tiger prawn (Penaeus monodon). An analysis of a 1158 bp DNA fragment 5′ upstream of the GIH gene revealed a putative half-site of the hormone response element (HRE) at −561 to −566 relative to the predicted transcription start site with an upstream imperfect palindromic sequence separated by one nucleotide. Deletion of the HRE from the GIH 5′ fragment increased the promoter activity in Sf9 cells, whereas the addition of 20-hydroxyecdysone (20E) reduced the promoter activity by approximately 60–70%. A candidate HRE-binding nuclear receptor, an ecdysone receptor (EcR) that serves to bind 20E in crustaceans, was therefore functionally characterized. EcR was expressed ubiquitously in female prawns, with the highest expression levels occurring in eyestalks, suggesting that EcR possibly stimulates vitellogenesis by binding to the HRE in the GIH 5′ regulatory sequence, thereby inhibiting GIH expression. This was verified by specific binding between a recombinant EcR-GST fusion protein and the HRE in the GIH 5′ DNA fragment. In addition, in vivo knockdown of EcR resulted in a 3-fold elevation of GIH expression levels in the eyestalks of adult female prawns. Taken together, the results obtained in this study indicate that EcR acts as an inhibitory factor in the regulation of the GIH gene.
    Keywords DNA fragmentation ; Penaeus monodon ; adults ; aquaculture ; ecdysone receptor ; ecdysterone ; females ; gene expression ; neurons ; peptides ; transcription (genetics) ; transcription initiation site ; vitellogenesis ; Gonad-inhibiting hormone ; Transcriptional regulation ; Reproduction ; Prawn
    Language English
    Dates of publication 2023-05
    Publishing place Elsevier B.V.
    Document type Article ; Online
    ZDB-ID 185380-6
    ISSN 0044-8486 ; 0044-8516
    ISSN 0044-8486 ; 0044-8516
    DOI 10.1016/j.aquaculture.2023.739393
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  3. Article ; Online: Cholesterol and phosphatidylserine are engaged in adenoviral dodecahedron endocytosis.

    Jedynak, Marta / Worch, Remigiusz / Podsiadła-Białoskórska, Małgorzata / Chroboczek, Jadwiga / Szołajska, Ewa

    Biochimica et biophysica acta. Biomembranes

    2018  Volume 1860, Issue 11, Page(s) 2215–2223

    Abstract: Adenoviral dodecahedron is a virus-like particle composed of twelve penton base proteins, derived from the capsid of human adenovirus type 3. Due to the high cell penetration capacity, it was used as a vector for protein, peptide and drug delivery. Two ... ...

    Abstract Adenoviral dodecahedron is a virus-like particle composed of twelve penton base proteins, derived from the capsid of human adenovirus type 3. Due to the high cell penetration capacity, it was used as a vector for protein, peptide and drug delivery. Two receptors are known to be involved in the endocytic dodecahedron uptake, namely αv integrins and heparan sulfate proteoglycans. Since it has been observed, that dodecahedron efficiently penetrates a wide range of cancer cells, it suggests that other cellular compounds may play a role in the particle endocytosis. To shed some light onto the interactions with membrane lipids and their potential role in dodecahedron entry, we performed a series of experiments including biochemical assays, fluorescence confocal imaging of giant unilamellar vesicles and surface plasmon resonance, which indicated specific preference of the particle to anionic phosphatidylserine. Experiments performed on cholesterol-depleted epithelial cells showed that cholesterol is essential in the endocytic uptake, however a direct interaction was not observed. We believe that the results will allow to better understand the role of lipids in dodecahedron entry and to design more specific dodecahedron-based vectors for drug delivery to cancer cells.
    MeSH term(s) Adenoviruses, Human/metabolism ; Annexin A5/metabolism ; Cholesterol/metabolism ; Endocytosis ; HeLa Cells ; Humans ; Membrane Lipids/metabolism ; Phosphatidylserines/metabolism ; Surface Plasmon Resonance
    Chemical Substances Annexin A5 ; Membrane Lipids ; Phosphatidylserines ; Cholesterol (97C5T2UQ7J)
    Language English
    Publishing date 2018-09-07
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 60-7
    ISSN 1879-2642 ; 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    ISSN (online) 1879-2642 ; 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2618 ; 1879-2650
    ISSN 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    DOI 10.1016/j.bbamem.2018.09.002
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    In stock of ZB MED Cologne/Königswinter

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  4. Article: Effect of spatial position of uterine quail blastoderms cultured in vitro on bilateral symmetry formation.

    Olsrzańska, Bozenna / Szolajska, Ewa / Lassota, Zofia

    Wilhelm Roux's archives of developmental biology

    2017  Volume 193, Issue 2, Page(s) 108–110

    Abstract: A method of in vitro culture for uterine quail blastoderms has been developed, which allows them to develop from cleavage throughout gastrulation and further: stages 4-10 of Hamburger and Hamilton (1951). The method consists of cultivating the ... ...

    Abstract A method of in vitro culture for uterine quail blastoderms has been developed, which allows them to develop from cleavage throughout gastrulation and further: stages 4-10 of Hamburger and Hamilton (1951). The method consists of cultivating the blastoderms on egg albumen in a vertical position; this permits about 50% of the blastoderms explanted before area pellucida formation to develop bilateral symmetry and to form normal primitive streak, somites and head structures. Development of the blastoderms explanted after their area pellucida was already formed, occurred normally and was not influenced by their spatial position in the culture.
    Language English
    Publishing date 2017-03-16
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 191268-9
    ISSN 0340-0794
    ISSN 0340-0794
    DOI 10.1007/BF00848638
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    In stock of ZB MED Cologne/Königswinter

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  5. Article ; Online: Effect of Posttranslational Modifications on the Structure and Activity of FTO Demethylase.

    Marcinkowski, Michał / Pilžys, Tomaš / Garbicz, Damian / Piwowarski, Jan / Mielecki, Damian / Nowaczyk, Grzegorz / Taube, Michał / Gielnik, Maciej / Kozak, Maciej / Winiewska-Szajewska, Maria / Szołajska, Ewa / Dębski, Janusz / Maciejewska, Agnieszka M / Przygońska, Kaja / Ferenc, Karolina / Grzesiuk, Elżbieta / Poznański, Jarosław

    International journal of molecular sciences

    2021  Volume 22, Issue 9

    Abstract: The FTO protein is involved in a wide range of physiological processes, including adipogenesis and osteogenesis. This two-domain protein belongs to the AlkB family of 2-oxoglutarate (2-OG)- and Fe(II)-dependent dioxygenases, ... ...

    Abstract The FTO protein is involved in a wide range of physiological processes, including adipogenesis and osteogenesis. This two-domain protein belongs to the AlkB family of 2-oxoglutarate (2-OG)- and Fe(II)-dependent dioxygenases, displaying
    MeSH term(s) Alpha-Ketoglutarate-Dependent Dioxygenase FTO/genetics ; Alpha-Ketoglutarate-Dependent Dioxygenase FTO/metabolism ; Alpha-Ketoglutarate-Dependent Dioxygenase FTO/physiology ; Catalysis ; Catalytic Domain ; Dioxygenases/genetics ; Humans ; Ketoglutaric Acids/metabolism ; Protein Processing, Post-Translational/genetics ; RNA, Messenger/genetics ; Scattering, Small Angle ; Structure-Activity Relationship ; X-Ray Diffraction/methods
    Chemical Substances Ketoglutaric Acids ; RNA, Messenger ; Dioxygenases (EC 1.13.11.-) ; Alpha-Ketoglutarate-Dependent Dioxygenase FTO (EC 1.14.11.33) ; FTO protein, human (EC 1.14.11.33)
    Language English
    Publishing date 2021-04-26
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms22094512
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  6. Article ; Online: Faithful chaperones.

    Szolajska, Ewa / Chroboczek, Jadwiga

    Cellular and molecular life sciences : CMLS

    2011  Volume 68, Issue 20, Page(s) 3307–3322

    Abstract: This review describes the properties of some rare eukaryotic chaperones that each assist in the folding of only one target protein. In particular, we describe (1) the tubulin cofactors, (2) p47, which assists in the folding of collagen, (3) α-hemoglobin ... ...

    Abstract This review describes the properties of some rare eukaryotic chaperones that each assist in the folding of only one target protein. In particular, we describe (1) the tubulin cofactors, (2) p47, which assists in the folding of collagen, (3) α-hemoglobin stabilizing protein (AHSP), (4) the adenovirus L4-100 K protein, which is a chaperone of the major structural viral protein, hexon, and (5) HYPK, the huntingtin-interacting protein. These various-sized proteins (102-1,190 amino acids long) are all involved in the folding of oligomeric polypeptides but are otherwise functionally unique, as they each assist only one particular client. This raises a question regarding the biosynthetic cost of the high-level production of such chaperones. As the clients of faithful chaperones are all abundant proteins that are essential cellular or viral components, it is conceivable that this necessary metabolic expenditure withstood evolutionary pressure to minimize biosynthetic costs. Nevertheless, the complexity of the folding pathways in which these chaperones are involved results in error-prone processes. Several human disorders associated with these chaperones are discussed.
    MeSH term(s) Animals ; Humans ; Molecular Chaperones/physiology ; Protein Folding ; Proteins/physiology
    Chemical Substances Molecular Chaperones ; Proteins
    Language English
    Publishing date 2011-06-08
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 1358415-7
    ISSN 1420-9071 ; 1420-682X
    ISSN (online) 1420-9071
    ISSN 1420-682X
    DOI 10.1007/s00018-011-0740-4
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 195: Show issues Location:
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  7. Article ; Online: Virus-like particles as vaccine.

    Chroboczek, Jadwiga / Szurgot, Inga / Szolajska, Ewa

    Acta biochimica Polonica

    2014  Volume 61, Issue 3, Page(s) 531–539

    Abstract: This review presents data on commercial and experimental virus-like particle (VLP) vaccines, including description of VLP vaccines against influenza. Virus-like particles are multimeric, sometimes multiprotein nanostructures assembled from viral ... ...

    Abstract This review presents data on commercial and experimental virus-like particle (VLP) vaccines, including description of VLP vaccines against influenza. Virus-like particles are multimeric, sometimes multiprotein nanostructures assembled from viral structural proteins and are devoid of any genetic material. VLPs present repetitive high-density displays of viral surface proteins. Importantly, they contain functional viral proteins responsible for cell penetration by the virus, ensuring efficient cell entry and thus tissue-specific targeting, determined by the origin of the virus. The foremost application of VLPs is in vaccinology, where they provide delivery systems that combine good safety profiles with strong immunogenicity and constitute a safe alternative to inactivated infectious viruses. These stable and versatile nanoparticles display excellent adjuvant properties capable of inducing innate and cognate immune responses. They present both, high-density B-cell epitopes, for antibody production and intracellular T-cell epitopes, thus inducing, respectively, potent humoral and cellular immune responses. Uptake of VLPs by antigen-presenting cells leads to efficient immune responses resulting in control of pathogenic microorganisms.
    MeSH term(s) Epitopes, B-Lymphocyte/immunology ; Epitopes, T-Lymphocyte/immunology ; Humans ; Influenza A virus/genetics ; Influenza A virus/immunology ; Influenza A virus/ultrastructure ; Influenza, Human/prevention & control ; Nanostructures ; Vaccination ; Vaccines, Virus-Like Particle/immunology
    Chemical Substances Epitopes, B-Lymphocyte ; Epitopes, T-Lymphocyte ; Vaccines, Virus-Like Particle
    Language English
    Publishing date 2014
    Publishing country Poland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 595762-x
    ISSN 1734-154X ; 0001-527X
    ISSN (online) 1734-154X
    ISSN 0001-527X
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    In stock of ZB MED Cologne/Königswinter

    Zs.B 232: Show issues Location:
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  8. Article ; Online: On the use of adenovirus dodecahedron as a carrier for glycoconjugate vaccines.

    Prasanna, Maruthi / Podsiadla-Bialoskorska, Malgorzata / Mielecki, Damian / Ruffier, Nicolas / Fateh, Amina / Lambert, Annie / Fanuel, Mathieu / Camberlein, Emilie / Szolajska, Ewa / Grandjean, Cyrille

    Glycoconjugate journal

    2021  Volume 38, Issue 4, Page(s) 437–446

    Abstract: Virus-Like Particles (VLPs) have been used as immunogenic molecules in numerous recombinant vaccines. VLPs can also serve as vaccine platform to exogenous antigens, usually peptides incorporated within the protein sequences which compose the VLPs or ... ...

    Abstract Virus-Like Particles (VLPs) have been used as immunogenic molecules in numerous recombinant vaccines. VLPs can also serve as vaccine platform to exogenous antigens, usually peptides incorporated within the protein sequences which compose the VLPs or conjugated to them. We herein described the conjugation of a synthetic tetrasaccharide mimicking the Streptococcus pneumoniae serotype 14 capsular polysaccharide to recombinant adenoviral type 3 dodecahedron, formed by the self-assembling of twelve penton bases and investigated the induced immune response when administered subcutaneously (s.c.). Whether formulated in the form of a dodecahedron or disassembled, the glycoconjugate induced an anti-protein response after two and three immunizations equivalent to that observed when the native dodecahedron was administered. On the other hand, the glycoconjugate induced a weak anti-IgM response which diminishes after two doses but no IgM-to-IgG switch was observed in mice against the serotype 14 capsular polysaccharide. In definitive, the whole conjugation process preserved both particulate nature and immunogenicity of the adenoviral dodecahedron. Further studies are needed to fully exploit adenoviral dodecahedron potential in terms of plasticity towards sequence engineering and of its capacity to stimulate the immune system via the intranasal route of administration as well as to shift the response to the carbohydrate antigen by playing both with the carbohydrate to protein ratio and the length of the synthetic carbohydrate antigen.
    MeSH term(s) Adenoviridae ; Glycoconjugates/chemistry ; Models, Molecular ; Pneumococcal Vaccines/chemistry ; Pneumococcal Vaccines/immunology ; Protein Conformation ; Streptococcus pneumoniae ; Vaccines, Conjugate/chemistry ; Vaccines, Conjugate/immunology
    Chemical Substances Glycoconjugates ; Pneumococcal Vaccines ; Vaccines, Conjugate
    Language English
    Publishing date 2021-04-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 283770-5
    ISSN 1573-4986 ; 0282-0080
    ISSN (online) 1573-4986
    ISSN 0282-0080
    DOI 10.1007/s10719-021-09999-3
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2171: Show issues Location:
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  9. Article ; Online: Effect of Posttranslational Modifications on the Structure and Activity of FTO Demethylase

    Michał Marcinkowski / Tomaš Pilžys / Damian Garbicz / Jan Piwowarski / Damian Mielecki / Grzegorz Nowaczyk / Michał Taube / Maciej Gielnik / Maciej Kozak / Maria Winiewska-Szajewska / Ewa Szołajska / Janusz Dębski / Agnieszka M. Maciejewska / Kaja Przygońska / Karolina Ferenc / Elżbieta Grzesiuk / Jarosław Poznański

    International Journal of Molecular Sciences, Vol 22, Iss 4512, p

    2021  Volume 4512

    Abstract: The FTO protein is involved in a wide range of physiological processes, including adipogenesis and osteogenesis. This two-domain protein belongs to the AlkB family of 2-oxoglutarate (2-OG)- and Fe(II)-dependent dioxygenases, displaying N 6 - ... ...

    Abstract The FTO protein is involved in a wide range of physiological processes, including adipogenesis and osteogenesis. This two-domain protein belongs to the AlkB family of 2-oxoglutarate (2-OG)- and Fe(II)-dependent dioxygenases, displaying N 6 -methyladenosine ( N 6 -meA) demethylase activity. The aim of the study was to characterize the relationships between the structure and activity of FTO. The effect of cofactors (Fe 2+ /Mn 2+ and 2-OG), Ca 2+ that do not bind at the catalytic site, and protein concentration on FTO properties expressed in either E. coli ( EC FTO) or baculovirus ( BES FTO) system were determined using biophysical methods (DSF, MST, SAXS) and biochemical techniques (size-exclusion chromatography, enzymatic assay). We found that BES FTO carries three phosphoserines (S184, S256, S260), while there were no such modifications in EC FTO. The S256D mutation mimicking the S256 phosphorylation moderately decreased FTO catalytic activity. In the presence of Ca 2+ , a slight stabilization of the FTO structure was observed, accompanied by a decrease in catalytic activity. Size exclusion chromatography and MST data confirmed the ability of FTO from both expression systems to form homodimers. The MST-determined dissociation constant of the FTO homodimer was consistent with their in vivo formation in human cells. Finally, a low-resolution structure of the FTO homodimer was built based on SAXS data.
    Keywords EC FTO ; BES FTO ; phosphorylation ; calcium ; dimerization ; nanoDSF ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 333
    Language English
    Publishing date 2021-04-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Leukocytes and drug-resistant cancer cells are targets for intracellular delivery by adenoviral dodecahedron.

    Jedynak, Marta / Laurin, David / Dolega, Patryk / Podsiadla-Bialoskorska, Malgorzata / Szurgot, Inga / Chroboczek, Jadwiga / Szolajska, Ewa

    Nanomedicine : nanotechnology, biology, and medicine

    2018  Volume 14, Issue 6, Page(s) 1853–1865

    Abstract: One of the major factors limiting the effectiveness of cancer chemotherapy is inefficient drug delivery. Systems enabling efficient delivery and enhanced intracellular uptake appear particularly promising in this respect. Virus-like particle, adenoviral ... ...

    Abstract One of the major factors limiting the effectiveness of cancer chemotherapy is inefficient drug delivery. Systems enabling efficient delivery and enhanced intracellular uptake appear particularly promising in this respect. Virus-like particle, adenoviral dodecahedron (Dd), employs receptor-mediated endocytosis for cell penetration and is able to deliver intracellularly dozens of cargo molecules attached to one particle. We focused on studying Dd properties in the context of cancer treatment, showing that intratumoral injection of Dd, assessed in mouse xenograft model, results in vector accumulation in tumor without spreading in off-target organs. Moreover, we demonstrated that Dd is a promising vector targeting leukocytes and drug-resistant cancer cells. Dd uptake by human blood cells analyzed in vitro indicated the preference for leukocytes in comparison to red blood cells and platelets. Furthermore, internalization of Dd-doxorubicin conjugate by drug-resistant cells leads to increased nuclear accumulation of doxorubicin and significant enhancement of cytotoxicity against target cancer cells.
    MeSH term(s) Adenoviridae/genetics ; Animals ; Antibiotics, Antineoplastic/pharmacology ; Capsid Proteins/administration & dosage ; Capsid Proteins/genetics ; Cells, Cultured ; Doxorubicin/pharmacology ; Drug Delivery Systems ; Drug Resistance, Neoplasm ; Humans ; Leukocytes/cytology ; Leukocytes/metabolism ; Mice ; Neoplasms/therapy
    Chemical Substances Antibiotics, Antineoplastic ; Capsid Proteins ; Doxorubicin (80168379AG)
    Language English
    Publishing date 2018-05-17
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2183417-9
    ISSN 1549-9642 ; 1549-9634
    ISSN (online) 1549-9642
    ISSN 1549-9634
    DOI 10.1016/j.nano.2018.05.001
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