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Article ; Online: Angiokeratoma of tongue in the pediatric age group: a rare presentation.

Jakhar, Rohit Kumar / Kakkar, Vikas / Sharma, Akriti / Stani, Anusha / Joy, Divya / Agrawal, Ayushi / Singh, Sumit

European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery

2024

Abstract: Background: Angiokeratoma is a rare cutaneous presentation with unknown etiology.: Case presentation: A case of a 10-year male, who was presented to the ENT OPD with a swelling over the posterior aspect of the tongue. The chief complaints included ... ...

Abstract	Background: Angiokeratoma is a rare cutaneous presentation with unknown etiology. Case presentation: A case of a 10-year male, who was presented to the ENT OPD with a swelling over the posterior aspect of the tongue. The chief complaints included growth on the right side of the posterior third of the tongue which was extending up to the base of the tongue on the same side. Conclusion: Excisional biopsy was taken and sent for histopathology which was suggestive of inflamed angiokeratoma. Post-excision there is no recurrence till date.
Language	English
Publishing date	2024-03-27
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1017359-6
ISSN	1434-4726 ; 0937-4477
ISSN (online)	1434-4726
ISSN	0937-4477
DOI	10.1007/s00405-024-08575-3
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Article: IgG responses against SARS-CoV-2 vaccines AZD1222 and BBV-152 and breakthrough infections among health care workers in southern India.

Rohit, Anusha / DSouza, Caroline / Kumar, Suresh / Ct, Meenachi / V, Vinothini / Perumal, Siva / Philip, M / George, Raju / Karunasagar, Iddya

Heliyon

2024 Volume 10, Issue 3, Page(s) e25528

Abstract: IgG antibodies elicited in response to SARS-CoV-2 are critical in determining the protection achieved through vaccination. The present longitudinal study aims to assess the immune response generated through AZD1222 & BBV-152 vaccination among health care ...

Abstract	IgG antibodies elicited in response to SARS-CoV-2 are critical in determining the protection achieved through vaccination. The present longitudinal study aims to assess the immune response generated through AZD1222 & BBV-152 vaccination among health care workers (HCWs) in a selected hospital. Serum IgG levels were measured approximately at 1.5 months and 6 months after the first and second vaccination. The final assessment was done 12 months after the first vaccination to analyse the sustained antibody levels. Results showed a progressive increase in antibody titres as a function of time. 26 HCWs in all had SARS-CoV-2 breakthrough infection, but their antibody titres were not significantly higher compared to COVID-19 naïve individuals. However, a comparative analysis showed considerably higher antibody titre in those who received the AZD1222 vaccine among this cohort. AZD1222 vaccination was significantly associated with seropositivity in the first and second assessments. Female HCWs showed significantly higher seropositivity, and participants above 60 years showed considerably reduced antibody titre in the first assessment. However, the final assessment showed no association with these variables, with 97.1 % of participants reporting to be seropositive. The results indicate good antibody response and potential protection against SARS CoV-2.
Language	English
Publishing date	2024-01-30
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2024.e25528
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Article ; Online: Differential expression of outer membrane proteins and quinolone resistance determining region mutations can lead to ciprofloxacin resistance in Salmonella Typhi.

Akshay, Sadanand Dangari / Nayak, Srajana / Deekshit, Vijaya Kumar / Rohit, Anusha / Maiti, Biswajit

Archives of microbiology

2023 Volume 205, Issue 4, Page(s) 136

Abstract: Multi-drug resistance in Salmonella Typhi remains a public health concern globally. This study aimed to investigate the function of quinolone resistance determining region (QRDR) of gyrA and parC in ciprofloxacin (CIP) resistant isolates and examine the ... ...

Abstract	Multi-drug resistance in Salmonella Typhi remains a public health concern globally. This study aimed to investigate the function of quinolone resistance determining region (QRDR) of gyrA and parC in ciprofloxacin (CIP) resistant isolates and examine the differential expression of outer membrane proteins (OMPs) on exposure to sub-lethal concentrations of CIP in S. Typhi. The CIP-resistant isolates were screened for mutations in the QRDR and analyzed for bacterial growth. Furthermore, major OMPs encoding genes such as ompF, lamB, yaeT, tolC, ompS1, and phoE were examined for differential expression under the sub-lethal concentrations of CIP by real-time PCR and SDS-PAGE. Notably, our study has shown a single-point mutation in gyrA at codon 83 (Ser83-tyrosine and Ser83-phenylalanine), also the rare amino acid substitution in parC gene at codon 80 (Glu80-glycine) in CIP-resistant isolates. Additionally, CIP-resistant isolates showed moderate growth compared to susceptible isolates. Although most of the OMP-encoding genes (tolC, ompS1, and phoE) showed some degree of upregulation, a significant level of upregulation (p < 0.05) was observed only for yaeT. However, ompF and lamB genes were down-regulated compared to CIP-susceptible isolates. Whereas OMPs profiling using SDS-PAGE did not show any changes in the banding pattern. These results provide valuable information on the QRDR mutation, and the difference in the growth, and expression of OMP-encoding genes in resistant and susceptible isolates of S. Typhi. This further provides insight into the involvement of QRDR mutation and OMPs associated with CIP resistance in S. Typhi.
MeSH term(s)	Ciprofloxacin/pharmacology ; Salmonella typhi/genetics ; Quinolones/pharmacology ; Anti-Bacterial Agents/pharmacology ; Membrane Proteins/genetics ; Mutation ; DNA Gyrase/genetics ; Drug Resistance, Bacterial/genetics ; Microbial Sensitivity Tests
Chemical Substances	Ciprofloxacin (5E8K9I0O4U) ; Quinolones ; Anti-Bacterial Agents ; Membrane Proteins ; DNA Gyrase (EC 5.99.1.3)
Language	English
Publishing date	2023-03-24
Publishing country	Germany
Document type	Journal Article
ZDB-ID	124824-8
ISSN	1432-072X ; 0302-8933
ISSN (online)	1432-072X
ISSN	0302-8933
DOI	10.1007/s00203-023-03485-0
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Article ; Online: Peritoneal dialysis related peritonitis due to Mycobacterium spp.

Anusha Rohit / Georgi Abraham

Journal of Epidemiology and Global Health, Vol 6, Iss

A case report and review of literature

2019 Volume 4

Abstract: Mycobacterium tuberculous peritonitis is a less common cause of peritoneal dialysis related infection in developed countries. As both CAPD and APD are being used as renal replacement therapy in developing countries of South Asia, Mycobacterium ... ...

Abstract	Mycobacterium tuberculous peritonitis is a less common cause of peritoneal dialysis related infection in developed countries. As both CAPD and APD are being used as renal replacement therapy in developing countries of South Asia, Mycobacterium tuberculous peritonitis are being reported. Any culture negative peritonitis should be investigated for this entity. In this manuscript, we report an index case and our experience with literature review of Mycobacterium tuberculous peritonitis. The diagnostic techniques, management and outcome are described.
Keywords	Chronic kidney disease ; Diagnosis ; Tuberculous peritonitis ; Public aspects of medicine ; RA1-1270
Language	English
Publishing date	2019-04-01T00:00:00Z
Publisher	Springer
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Successful Automated Peritoneal Dialysis (APD) in a COVID-19 patient with acalculous pancreatitis with no detectable virus in the dialysate effluent.

Abraham, Georgi / Rohit, Anusha / Mathew, Milly / Parthasarathy, Rajeevalochana

Indian journal of medical microbiology

2020 Volume 39, Issue 1, Page(s) 128–129

MeSH term(s)	Automation ; Biomarkers ; COVID-19/complications ; COVID-19/diagnosis ; COVID-19/virology ; Disease Management ; Female ; Humans ; Kidney Failure, Chronic/complications ; Middle Aged ; Pancreatitis/complications ; Pancreatitis/therapy ; Peritoneal Dialysis/methods ; SARS-CoV-2 ; Symptom Assessment ; Tomography, X-Ray Computed ; Treatment Outcome
Chemical Substances	Biomarkers
Language	English
Publishing date	2020-11-03
Publishing country	United States
Document type	Case Reports
ZDB-ID	1038798-5
ISSN	1998-3646 ; 0255-0857
ISSN (online)	1998-3646
ISSN	0255-0857
DOI	10.1016/j.ijmmb.2020.10.010
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Article: COVID-19 Infection in CAPD Patients: A Single-Center Indian Experience.

Parthasarathy, Rajeevalochana / David, Vinoi George / Rohit, Anusha / Mathew, Milly / Sabu, Sandra / Babu, Madhu / Abraham, Georgi

Indian journal of nephrology

2022 Volume 32, Issue 2, Page(s) 186–188

Language	English
Publishing date	2022-01-05
Publishing country	India
Document type	Journal Article
ZDB-ID	2134388-3
ISSN	1998-3662 ; 0971-4065
ISSN (online)	1998-3662
ISSN	0971-4065
DOI	10.4103/ijn.IJN_522_20
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Article ; Online: Differential expression of outer membrane proteins and quinolone resistance determining region mutations can lead to ciprofloxacin resistance in Salmonella Typhi

Akshay, Sadanand Dangari / Nayak, Srajana / Deekshit, Vijaya Kumar / Rohit, Anusha / Maiti, Biswajit

Arch Microbiol. 2023 Apr., v. 205, no. 4 p.136-136

2023

Abstract	Multi-drug resistance in Salmonella Typhi remains a public health concern globally. This study aimed to investigate the function of quinolone resistance determining region (QRDR) of gyrA and parC in ciprofloxacin (CIP) resistant isolates and examine the differential expression of outer membrane proteins (OMPs) on exposure to sub-lethal concentrations of CIP in S. Typhi. The CIP-resistant isolates were screened for mutations in the QRDR and analyzed for bacterial growth. Furthermore, major OMPs encoding genes such as ompF, lamB, yaeT, tolC, ompS1, and phoE were examined for differential expression under the sub-lethal concentrations of CIP by real-time PCR and SDS-PAGE. Notably, our study has shown a single-point mutation in gyrA at codon 83 (Ser83-tyrosine and Ser83-phenylalanine), also the rare amino acid substitution in parC gene at codon 80 (Glu80-glycine) in CIP-resistant isolates. Additionally, CIP-resistant isolates showed moderate growth compared to susceptible isolates. Although most of the OMP-encoding genes (tolC, ompS1, and phoE) showed some degree of upregulation, a significant level of upregulation (p < 0.05) was observed only for yaeT. However, ompF and lamB genes were down-regulated compared to CIP-susceptible isolates. Whereas OMPs profiling using SDS-PAGE did not show any changes in the banding pattern. These results provide valuable information on the QRDR mutation, and the difference in the growth, and expression of OMP-encoding genes in resistant and susceptible isolates of S. Typhi. This further provides insight into the involvement of QRDR mutation and OMPs associated with CIP resistance in S. Typhi.
Keywords	Salmonella Typhi ; amino acid substitution ; bacterial growth ; ciprofloxacin ; gene expression regulation ; genes ; multiple drug resistance ; polyacrylamide gel electrophoresis ; public health ; quantitative polymerase chain reaction
Language	English
Dates of publication	2023-04
Size	p. 136.
Publishing place	Springer Berlin Heidelberg
Document type	Article ; Online
ZDB-ID	124824-8
ISSN	1432-072X ; 0302-8933
ISSN (online)	1432-072X
ISSN	0302-8933
DOI	10.1007/s00203-023-03485-0
Database	NAL-Catalogue (AGRICOLA)

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Article: Prophage and Plasmid-Mediated Beta-Lactamases in Multidrug-Resistant Extraintestinal Escherichia coli

Raj, Juliet Roshini Mohan / Dinesh, Akshatha / Vittal, Rajeshwari / Rohit, Anusha

Journal of Health and Allied Sciences NU

2023

Abstract: Objectives: Antibiotic resistance can arise as a mutation to adapt to stress or be mediated by horizontal gene transfer. This study aimed at identifying the resistance determinants present in the mobile genetic elements of prophages and plasmids within ... ...

Abstract	Objectives: Antibiotic resistance can arise as a mutation to adapt to stress or be mediated by horizontal gene transfer. This study aimed at identifying the resistance determinants present in the mobile genetic elements of prophages and plasmids within multidrug-resistant (MDR) extraintestinal Escherichia coli Materials and Methods: Thirty-five anonymized MDR E. coli isolates of nonintestinal infections were confirmed for their antimicrobial resistance to six categories of antimicrobials by the disk diffusion test. Genes coding for beta-lactamases and carbapenemases in bacterial genome, plasmid, and prophage fractions were separately determined by polymerase chain reaction. Transducing ability of prophages carrying resistance genes was determined. Results: Twenty-six isolates were positive for the gene bla CTX-M, nine for bla TEM, one each for bla KPC and bla VIM, thirteen for bla NDM, and seven for bla OXA. A majority of these isolates carried these determinants in plasmids and prophage fractions. Twenty-one percent of the prophage fractions (4 of 19) were able to successfully transfer resistance to sensitive isolates. Conclusions: This study indicates bla CTX-M bla TEM, and bla NDM genes that are reported most frequently in MDR isolates are more frequent in the plasmid and prophage fractions thus supporting for increased mobility.
Keywords	beta-lactamase ; carbapenemases ; mobile genetic elements ; MDR
Language	English
Publishing date	2023-08-24
Publisher	Thieme Medical and Scientific Publishers Pvt. Ltd.
Publishing place	Stuttgart ; New York
Document type	Article
ISSN	2582-4953 ; 2582-4287
ISSN (online)	2582-4953
ISSN	2582-4287
DOI	10.1055/s-0043-1772707
Database	Thieme publisher's database

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Article ; Online: Fate of respiratory droplets in tropical vs temperate environments and implications for SARS-CoV-2 transmission.

Rohit, Anusha / Rajasekaran, Shankar / Karunasagar, Indrani / Karunasagar, Iddya

Medical hypotheses

2020 Volume 144, Page(s) 109958

Abstract: The new pandemic of SARS-CoV-2 has shown stark differences in number of affected patients between countries in the tropics and those with temperate environments. Though there have been many theories on reasons for these differences, we hypothesise that ... ...

Abstract	The new pandemic of SARS-CoV-2 has shown stark differences in number of affected patients between countries in the tropics and those with temperate environments. Though there have been many theories on reasons for these differences, we hypothesise that this could be due to differences in the fate of respiratory droplets in the two environments. A simple understanding of the mechanics of droplet size, dispersion and displacement could help infection control and public health measures to minimize spread and mitigate the risk of people getting infected especially in hotspots like hospital environments or other closed spaces. This paper discusses the possibility of differences in number of infections and spread between different countries based on the spread of droplets.
MeSH term(s)	Air Microbiology ; Air Movements ; COVID-19/epidemiology ; COVID-19/transmission ; Disease Transmission, Infectious ; Environment ; Geography, Medical ; Global Health ; Humans ; Humidity ; Meteorological Concepts ; Pandemics ; Particle Size ; Rheology ; SARS-CoV-2/isolation & purification ; Temperature ; Tropical Climate
Keywords	covid19
Language	English
Publishing date	2020-06-09
Publishing country	United States
Document type	Journal Article
ZDB-ID	193145-3
ISSN	1532-2777 ; 0306-9877
ISSN (online)	1532-2777
ISSN	0306-9877
DOI	10.1016/j.mehy.2020.109958
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Article ; Online: Effect of sub-minimum inhibitory concentration of ceftriaxone on the expression of outer membrane proteins in Salmonella enterica serovar Typhi.

Akshay, Sadanand Dangari / Anupama, Karanth Padyana / Deekshit, Vijaya Kumar / Rohit, Anusha / Maiti, Biswajit

World journal of microbiology & biotechnology

2022 Volume 38, Issue 11, Page(s) 190

Abstract: Multi-drug resistance (MDR) in Salmonella is one of the major reasons for foodborne outbreaks worldwide. Decreased susceptibility of Salmonella Typhi to first-line drugs such as ceftriaxone, ciprofloxacin, and azithromycin has raised concern. Reduced ... ...

Abstract	Multi-drug resistance (MDR) in Salmonella is one of the major reasons for foodborne outbreaks worldwide. Decreased susceptibility of Salmonella Typhi to first-line drugs such as ceftriaxone, ciprofloxacin, and azithromycin has raised concern. Reduced outer membrane proteins (OMPs) permeability and increased efflux pump transportation are considered to be the main reasons for the emergence of antibiotic resistance in Salmonella. The present study aimed to assess the expression of OMPs at sub-lethal concentrations of ceftriaxone in S. Typhi (Sl5037/BC, and Sl05). The S. Typhi strains were exposed to sub-MIC and half of the sub-MIC concentrations of ceftriaxone at three different time intervals (0 min, 40 min, and 180 min) and analyzed for differential expression of OMPs. Further, the expression variation of OMP encoding genes (yaeT, ompX, lamb, ompA, and ybfM) in response to ceftriaxone was evaluated using real-time PCR. The genes like lamB, ompX, and yaeT showed significant downregulation (p < 0.05) compared to the control without antibiotic exposure, whereas ybfM and ompA showed a moderate downregulation. The expression of omp genes such as lamB, ompA, ompX, ybfM, and yaeT were found to be low in the presence of ceftriaxone, followed by time and dose-dependent. The study provides insights into the possible involvement of OMPs in drug resistance of S. Typhi, which could help develop a therapeutic strategy to combat MDR isolates of S. Typhi.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Bacterial Outer Membrane Proteins/metabolism ; Ceftriaxone/pharmacology ; Drug Resistance, Bacterial ; Microbial Sensitivity Tests ; Salmonella typhi/drug effects
Chemical Substances	Anti-Bacterial Agents ; Bacterial Outer Membrane Proteins ; Ceftriaxone (75J73V1629)
Language	English
Publishing date	2022-08-16
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1499109-3
ISSN	1573-0972 ; 0959-3993
ISSN (online)	1573-0972
ISSN	0959-3993
DOI	10.1007/s11274-022-03383-5
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