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Article ; Online: Dual Role for l-[Methyl-

Haberbosch, Linus / Gillett, Daniel / MacFarlane, James / Koulouri, Olympia / Mai, Knut / Spranger, Joachim / Mannion, Richard / Cheow, Heok / Jones, Jonathan / Gurnell, Mark

Journal of nuclear medicine : official publication, Society of Nuclear Medicine

2024 Volume 65, Issue 2, Page(s) 327–328

MeSH term(s)	Humans ; Methionine ; Acromegaly/diagnostic imaging ; Racemethionine ; Brain Neoplasms ; Positron-Emission Tomography
Chemical Substances	Methionine (AE28F7PNPL) ; Racemethionine (73JWT2K6T3)
Language	English
Publishing date	2024-02-01
Publishing country	United States
Document type	Journal Article
ZDB-ID	80272-4
ISSN	1535-5667 ; 0097-9058 ; 0161-5505 ; 0022-3123
ISSN (online)	1535-5667
ISSN	0097-9058 ; 0161-5505 ; 0022-3123
DOI	10.2967/jnumed.123.266446
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Zs.A 114: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 328: Show issues

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Article ; Online: Discovery and characterization of l-DOPA 2,3-dioxygenase from Streptomyces hygroscopicus jingganensis.

Ringenbach, Sara / Yoza, Riri / Jones, Paige A / Du, Muxue / Klugh, Kameron L / Peterson, Larryn W / Colabroy, Keri L

Archives of biochemistry and biophysics

2024 Volume 755, Page(s) 109967

Abstract: ... enzymes. The EDX l-DOPA 2,3-dioxygenase was first discovered as part of a biosynthetic gene cluster ... tomaymycin, porothramycin and hormaomycin. Using these l-DOPA 2,3-dioxygenases as a starting point ... of the S. hygroscopicus jingganensis VOC-family-protein with respect to activity of l-DOPA 2,3-dioxygenases ...

Abstract	The largest natural reservoir of untapped carbon can be found in the cell-wall strengthening, plant woody-tissue polymer, lignin - a polymer of catechols or 1,2-dihydroxybenzene monomers. The catecholic carbon of lignin could be valorized into feedstocks and natural products by way of catabolic and biosynthetic transformations, including the oxygen-dependent cleavage reaction of extradiol dioxygenase (EDX) enzymes. The EDX l-DOPA 2,3-dioxygenase was first discovered as part of a biosynthetic gene cluster to the natural product antibiotic, lincomycin, and also contributes to the biosyntheses of anthramycin, sibiromycin, tomaymycin, porothramycin and hormaomycin. Using these l-DOPA 2,3-dioxygenases as a starting point, we searched sequence space in order to identify new sources of dioxygenase driven natural product diversity. A "vicinal-oxygen-chelate (VOC) family protein" from Streptomyces hygroscopicus jingganensis was identified using bioinformatic methods and biochemically investigated for dioxygenase activity against a suite of natural and synthetic catechols. Steady-state oxygen consumption assays were used to screen and identify substrates, and a steady-state kinetic model of oxygen consumption was developed to evaluate activity of the S. hygroscopicus jingganensis VOC-family-protein with respect to activity of l-DOPA 2,3-dioxygenases from Streptomyces lincolnensis and Streptomyces sclerotialus. Lastly, these data were integrated with steady-state kinetic methods to observe the formation of the EDX cleavage product with UV-visible spectroscopy. The genomic context and enzymatic activity of the S. hygroscopicus jingganensis VOC family protein are consistent with a l-DOPA 2,3-dioxygenase contained within a cryptic biosynthetic pathway.
Language	English
Publishing date	2024-03-30
Publishing country	United States
Document type	Journal Article
ZDB-ID	523-x
ISSN	1096-0384 ; 0003-9861
ISSN (online)	1096-0384
ISSN	0003-9861
DOI	10.1016/j.abb.2024.109967
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Article ; Online: Update to RIFM fragrance ingredient safety assessment, l-carvone, CAS Registry Number 6485-40-1.

Api, A M / Bartlett, A / Belsito, D / Botelho, D / Bruze, M / Bryant-Freidrich, A / Burton, G A / Cancellieri, M A / Chon, H / Dagli, M L / Dekant, W / Deodhar, C / Farrell, K / Fryer, A D / Jones, L / Joshi, K / Lapczynski, A / Lavelle, M / Lee, I /

Moustakas, H / Muldoon, J / Penning, T M / Ritacco, G / Sadekar, N / Schember, I / Schultz, T W / Siddiqi, F / Sipes, I G / Sullivan, G / Thakkar, Y / Tokura, Y

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

2024 Volume 183 Suppl 1, Page(s) 114505

MeSH term(s)	Odorants ; Perfume/toxicity ; Cyclohexane Monoterpenes
Chemical Substances	carvone (75GK9XIA8I) ; Perfume ; Cyclohexane Monoterpenes
Language	English
Publishing date	2024-02-10
Publishing country	England
Document type	Journal Article
ZDB-ID	782617-5
ISSN	1873-6351 ; 0278-6915
ISSN (online)	1873-6351
ISSN	0278-6915
DOI	10.1016/j.fct.2024.114505
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Article ; Online: Kidney function as a key driver of the pharmacokinetic response to high-dose L-carnitine in septic shock.

Jennaro, Theodore S / Puskarich, Michael A / Flott, Thomas L / McLellan, Laura A / Jones, Alan E / Pai, Manjunath P / Stringer, Kathleen A

Pharmacotherapy

2023 Volume 43, Issue 12, Page(s) 1240–1250

Abstract: Study objective: Levocarnitine (L-carnitine) has shown promise as a metabolic-therapeutic ... PK) of high-dose L-carnitine, test various estimates of kidney function, and assess the correlation ... Data source: We leveraged serum samples and metabolomics data from a phase II trial of L-carnitine ...

Abstract	Study objective: Levocarnitine (L-carnitine) has shown promise as a metabolic-therapeutic for septic shock, where mortality approaches 40%. However, high-dose (≥ 6 grams) intravenous supplementation results in a broad range of serum concentrations. We sought to describe the population pharmacokinetics (PK) of high-dose L-carnitine, test various estimates of kidney function, and assess the correlation of PK parameters with pre-treatment metabolites in describing drug response for patients with septic shock. Design: Population PK analysis was done with baseline normalized concentrations using nonlinear mixed effect models in the modeling platform Monolix. Various estimates of kidney function, patient demographics, dose received, and organ dysfunction were tested as population covariates. Data source: We leveraged serum samples and metabolomics data from a phase II trial of L-carnitine in vasopressor-dependent septic shock. Serum was collected at baseline (T0); end-of-infusion (T12); and 24, 48, and 72 h after treatment initiation. Patients and intervention: Patients were adaptively randomized to receive intravenous L-carnitine (6 grams, 12 grams, or 18 grams) or placebo. Measurements and main results: The final dataset included 542 serum samples from 130 patients randomized to L-carnitine. A two-compartment model with linear elimination and a fixed volume of distribution (17.1 liters) best described the data and served as a base structural model. Kidney function estimates as a covariate on the elimination rate constant (k) reliably improved model fit. Estimated glomerular filtration rate (eGFR), based on the 2021 Chronic Kidney Disease Epidemiology collaboration (CKD-EPI) equation with creatinine and cystatin C, outperformed creatinine clearance (Cockcroft-Gault) and older CKD-EPI equations that use an adjustment for self-identified race. Conclusions: High-dose L-carnitine supplementation is well-described by a two-compartment population PK model in patients with septic shock. Kidney function estimates that leverage cystatin C provided superior model fit. Future investigations into high-dose L-carnitine supplementation should consider baseline metabolic status and dose adjustments based on renal function over a fixed or weight-based dosing paradigm.
MeSH term(s)	Humans ; Cystatin C ; Carnitine ; Shock, Septic/drug therapy ; Creatinine ; Glomerular Filtration Rate/physiology ; Renal Insufficiency, Chronic ; Kidney
Chemical Substances	Cystatin C ; Carnitine (S7UI8SM58A) ; Creatinine (AYI8EX34EU)
Language	English
Publishing date	2023-10-11
Publishing country	United States
Document type	Randomized Controlled Trial ; Journal Article
ZDB-ID	603158-4
ISSN	1875-9114 ; 0277-0008
ISSN (online)	1875-9114
ISSN	0277-0008
DOI	10.1002/phar.2882
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Zs.A 1738: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 585: Show issues

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Article ; Online: Conséquences de la pandémie sur les politiques de l’éducation en Europe et le cas spécifique de l’Angleterre

Ken Jones

Cahiers du MIMMOC, Vol

2023 Volume 29

Abstract: ... nombre de chercheurs a tenté d’affiner l’articulation entre le global et le national. D’une part ... par lequel des politiques développées par l’OCDE, la Banque mondiale et le FMI ont été imposées aux états-nations. Un grand ... ont mis en évidence l’inévitable résistance de caractéristiques nationales ...

Abstract	Cela fait maintenant trente ans qu’existe un nouveau modèle de politique éducative, par lequel des politiques développées par l’OCDE, la Banque mondiale et le FMI ont été imposées aux états-nations. Un grand nombre de chercheurs a tenté d’affiner l’articulation entre le global et le national. D’une part, le fonctionnement d’un « complexe de gouvernance mondiale » a été largement démontré, d’autre part, des travaux ont mis en évidence l’inévitable résistance de caractéristiques nationales et le fait que les articulations entre le global et le national sont « divergentes et profondément enracinées dans l’histoire régionale et la politique contemporaine ». Cet article contribue à ce domaine de recherche complexe.L’impact du Covid-19 sur les systèmes éducatifs est abordé, en trois volets. Le premier de ces volets évoque les points communs qui ressortent des réactions des responsables politiques européens face aux effets de santé publique et sociaux de la pandémie. Dans un deuxième temps, s’appuyant sur l’exemple anglais, l’article vise à montrer la façon dont « l’histoire et les politiques régionales » infléchissent la stratégie nationale. Enfin, en présentant une analyse granulaire de la situation anglaise, qui prend en compte les pratiques d’action politique habituelles ainsi que les types de conflits qui s’y expriment, sont ainsi pris en compte des domaines de politique publique qui font l’objet de peu de travaux de recherche.
Keywords	système éducatif ; Covid-19 ; néo-libéralisme ; globalisme ; caractéristiques nationales ; Social Sciences ; H ; Social sciences (General) ; H1-99
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	Université de Poitiers
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: L-4-thiazolylalanine (Protinol), a novel non-proteinogenic amino acid, demonstrates epidermal and dermal efficacy with clinically observable benefits.

Portocarrero Huang, Gloria / Idkowiak-Baldys, Jolanta / Liebel, Frank / Jones, Constantina / Haxaire, Coline / DiNatale, Lisa / Bayat, Ardeshir / Glynn, John R

International journal of cosmetic science

2023 Volume 46, Issue 1, Page(s) 24–38

Abstract: ... and extrinsic factors. The goal of the current work is to demonstrate L-4-thiazolylalaine (L4 ...

Abstract	Objective: Facial skin undergoes major structural and functional changes as a result of intrinsic and extrinsic factors. The goal of the current work is to demonstrate L-4-thiazolylalaine (L4, Protinol), a non-proteinogenic amino acid shown to stimulate the production of dermal proteins by fibroblasts, is an alternative efficacious topical ingredient for visible signs of ageing. Methods: In vitro studies using 3D human skin tissue models were performed to show changes in protein and gene expression of key dermal markers in samples treated with 0.3% L4 compared to vehicle control. In vivo evaluation of skin turnover was measured in volunteers after treatment with L4 compared to retinol. Skin biopsies (n = 30) were taken to investigate epidermal and dermal changes in cases treated with L4 and compared to retinol. Finally, a clinical evaluation (n = 28) was conducted to assess the efficacy of L4 over a base formulation using various ageing parameters within a population of women 46-66 years old with mild-to-moderate wrinkles. Results: In vitro studies on 3D tissues displayed significant changes in the dermal matrix via an increase in HA and pro-collagen I production and a decrease in the expression of inflammatory genes. In vivo biopsy studies demonstrated that L4 and retinol independently increased epidermal thickness and collagen remodelling significantly more compared with the base formula. Clinical evaluation showed firmer and smoother skin at day 28 post-treatment with L4 over the vehicle control without causing side effects such as redness or irritation. Conclusion: L4 is a novel, multi-functional ingredient which offers a superior alternative to currently available technologies for improving epidermal and dermal parameters that change during ageing and photodamage.
MeSH term(s)	Humans ; Female ; Middle Aged ; Aged ; Vitamin A/pharmacology ; Amino Acids ; Skin/metabolism ; Epidermis/metabolism ; Collagen/metabolism ; Skin Aging
Chemical Substances	Vitamin A (11103-57-4) ; Amino Acids ; Collagen (9007-34-5)
Language	English
Publishing date	2023-12-20
Publishing country	England
Document type	Journal Article
ZDB-ID	198917-0
ISSN	1468-2494 ; 0142-5463
ISSN (online)	1468-2494
ISSN	0142-5463
DOI	10.1111/ics.12887
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Article ; Online: RIFM fragrance ingredient safety assessment, l-lactic acid (2-hydroxy propionic acid), CAS Registry Number 79-33-4.

Api, A M / Belsito, D / Botelho, D / Bruze, M / Burton, G A / Cancellieri, M A / Chon, H / Dagli, M L / Dekant, W / Deodhar, C / Fryer, A D / Jones, L / Joshi, K / Kumar, M / Lapczynski, A / Lavelle, M / Lee, I / Liebler, D C / Moustakas, H /

Muldoon, J / Penning, T M / Ritacco, G / Romine, J / Sadekar, N / Schultz, T W / Selechnik, D / Siddiqi, F / Sipes, I G / Sullivan, G / Thakkar, Y / Tokura, Y

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

2023 Volume 182 Suppl 1, Page(s) 114137

Language	English
Publishing date	2023-10-31
Publishing country	England
Document type	Journal Article
ZDB-ID	782617-5
ISSN	1873-6351 ; 0278-6915
ISSN (online)	1873-6351
ISSN	0278-6915
DOI	10.1016/j.fct.2023.114137
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Article ; Online: Microplastic accumulation in a Zostera marina L. bed at Deerness Sound, Orkney, Scotland.

Jones, Katherine L / Hartl, Mark G J / Bell, Michael C / Capper, Angela

Marine pollution bulletin

2020 Volume 152, Page(s) 110883

Abstract: Seagrasses have global distribution and are highly productive and economically valuable habitats. They are sensitive and vulnerable to a range of human-induced pressures, including ongoing exposure to marine litter, such as microplastic particles (<5 mm). ...

Abstract	Seagrasses have global distribution and are highly productive and economically valuable habitats. They are sensitive and vulnerable to a range of human-induced pressures, including ongoing exposure to marine litter, such as microplastic particles (<5 mm). In this study, a Zostera marina bed in Deerness Sound, Orkney was selected to determine whether microplastics accumulate in seagrass beds and adhere to seagrass blades. Sediment, seagrass blade, biota and seawater samples were collected. 280 microplastic particles (0.04 to 3.95 mm (mean = 0.95 mm ± 0.05 SE)) were observed in 94% of samples collected (n = 111). These were visually categorised into type (fibre, flake, fragment) and colour, and 50 were successfully identified as plastic using ATR-FTIR. Fibres contributed >50% of the total microplastics observed across all samples. This is the first known study on Z. marina to describe microplastic loading within a seagrass bed and to identify microplastic adherence to seagrass blades.
MeSH term(s)	Ecosystem ; Microplastics ; Plastics ; Scotland ; Zosteraceae
Chemical Substances	Microplastics ; Plastics
Language	English
Publishing date	2020-01-20
Publishing country	England
Document type	Journal Article
ZDB-ID	2001296-2
ISSN	1879-3363 ; 0025-326X
ISSN (online)	1879-3363
ISSN	0025-326X
DOI	10.1016/j.marpolbul.2020.110883
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Article ; Online: RIFM fragrance ingredient safety assessment, l-carvone, CAS Registry Number 6485-40-1.

Api, A M / Belsito, D / Botelho, D / Bruze, M / Burton, G A / Buschmann, J / Cancellieri, M A / Dagli, M L / Date, M / Dekant, W / Deodhar, C / Fryer, A D / Jones, L / Joshi, K / Kumar, M / Lapczynski, A / Lavelle, M / Lee, I / Liebler, D C /

Moustakas, H / Na, M / Penning, T M / Ritacco, G / Romine, J / Sadekar, N / Schultz, T W / Selechnik, D / Siddiqi, F / Sipes, I G / Sullivan, G / Thakkar, Y / Tokura, Y

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

2022 Volume 163 Suppl 1, Page(s) 112975

Abstract: l-Carvone was evaluated for genotoxicity, repeated dose toxicity, reproductive toxicity, local ... show that l-carvone is not genotoxic and provided a No Expected Sensitization Induction Level (NESIL ... of 2600 μg/cm2 for the skin sensitization endpoint. Data on l-carvone provided a calculated Margin ...

Abstract	l-Carvone was evaluated for genotoxicity, repeated dose toxicity, reproductive toxicity, local respiratory toxicity, phototoxicity/photoallergenicity, skin sensitization, and environmental safety. Data show that l-carvone is not genotoxic and provided a No Expected Sensitization Induction Level (NESIL) of 2600 μg/cm2 for the skin sensitization endpoint. Data on l-carvone provided a calculated Margin of Exposure (MOE) >100 for the repeated dose toxicity and reproductive toxicity endpoints. The phototoxicity/photoallergenicity endpoint was completed based on data and ultraviolet/visible (UV/Vis) spectra; l-carvone is not phototoxic/photoallergenic. The local respiratory toxicity endpoint was evaluated using the Threshold of Toxicological Concern (TTC) for a Cramer Class II material (0.47 mg/day); the exposure to l-carvone is below the TTC. The environmental endpoints were evaluated; l-carvone was found not to be persistent, bioaccumulative, and toxic (PBT) as per the International Fragrance Association (IFRA) Environmental Standards, and its risk quotients, based on its current volume of use in Europe and North America (i.e., Predicted Environmental Concentration/Predicted No Effect Concentration [PEC/PNEC]), are <1.
MeSH term(s)	Cyclohexane Monoterpenes ; Dermatitis, Phototoxic ; Humans ; Mutagenicity Tests ; Odorants ; Perfume/toxicity ; Registries ; Risk Assessment ; Toxicity Tests
Chemical Substances	Cyclohexane Monoterpenes ; Perfume ; carvone (75GK9XIA8I)
Language	English
Publishing date	2022-03-30
Publishing country	England
Document type	Journal Article
ZDB-ID	782617-5
ISSN	1873-6351 ; 0278-6915
ISSN (online)	1873-6351
ISSN	0278-6915
DOI	10.1016/j.fct.2022.112975
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Article ; Online: Tetraspanin CD53 Promotes Lymphocyte Recirculation by Stabilizing L-Selectin Surface Expression.

Demaria, Maria C / Yeung, Louisa / Peeters, Rens / Wee, Janet L / Mihaljcic, Masa / Jones, Eleanor L / Nasa, Zeyad / Alderuccio, Frank / Hall, Pamela / Smith, Brodie C / Binger, Katrina J / Hammerling, Gunther / Kwok, Hang Fai / Newman, Andrew / Ager, Ann / van Spriel, Annemiek / Hickey, Michael J / Wright, Mark D

iScience

2020 Volume 23, Issue 5, Page(s) 101104

Abstract: Tetraspanins regulate key processes in immune cells; however, the function of the leukocyte-restricted tetraspanin CD53 is unknown. Here we show that CD53 is essential for lymphocyte recirculation. Lymph nodes of ... ...

Abstract	Tetraspanins regulate key processes in immune cells; however, the function of the leukocyte-restricted tetraspanin CD53 is unknown. Here we show that CD53 is essential for lymphocyte recirculation. Lymph nodes of Cd53
Language	English
Publishing date	2020-04-27
Publishing country	United States
Document type	Journal Article
ISSN	2589-0042
ISSN (online)	2589-0042
DOI	10.1016/j.isci.2020.101104
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