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  1. Book ; Online ; Thesis: Vorkommen mutierter und nicht-mutierter feliner Coronaviren bei Katzen mit feliner infektiöser Peritonitis

    Emmler, Laura Aliki [Verfasser] / Hartmann, Katrin [Akademischer Betreuer]

    2020  

    Author's details Laura Aliki Emmler ; Betreuer: Katrin Hartmann
    Keywords Tiere (Zoologie) ; Animals (Zoology)
    Subject code sg590
    Language German
    Publisher Universitätsbibliothek der Ludwig-Maximilians-Universität
    Publishing place München
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  2. Article: Feline coronavirus with and without spike gene mutations detected by real-time RT-PCRs in cats with feline infectious peritonitis

    Emmler, Laura / Felten, Sandra / Matiasek, Kaspar / Balzer, Hans-Joerg / Pantchev, Nikola / Leutenegger, Christian / Hartmann, Katrin

    Journal of feline medicine and surgery. 2020 Aug., v. 22, no. 8

    2020  

    Abstract: Feline infectious peritonitis (FIP) emerges when feline coronaviruses (FCoVs) mutate within their host to a highly virulent biotype and the immune response is not able to control the infection. FCoV spike (S) gene mutations are considered to contribute ... ...

    Abstract Feline infectious peritonitis (FIP) emerges when feline coronaviruses (FCoVs) mutate within their host to a highly virulent biotype and the immune response is not able to control the infection. FCoV spike (S) gene mutations are considered to contribute to the change in virulence by enabling FCoV infection of and replication in macrophages. This study investigated the presence of FCoV with and without S gene mutations in cats with FIP using two different real-time RT-PCRs on different samples obtained under clinical conditions. Fine-needle aspirates (FNAs) and incisional biopsies (IBs) of popliteal and mesenteric lymph nodes, liver, spleen, omentum and kidneys (each n = 20), EDTA blood (n = 13), buffy coat smears (n = 13), serum (n = 11), effusion (n = 14), cerebrospinal fluid (n = 16), aqueous humour (n = 20) and peritoneal lavage (n = 6) were obtained from 20 cats with FIP diagnosed by immunohistochemistry. Samples were examined by RT-PCR targeting the FCoV 7b gene, detecting all FCoV, and S gene mutation RT-PCR targeting mutations in nucleotides 23531 and 23537. The prevalence of FCoV detected in each sample type was calculated. In 20/20 cats, FCoV with S gene mutations was present in at least one sample, but there was variation in which sample was positive. FCoV with mutations in the S gene was most frequently found in effusion (64%, 95% confidence interval [CI] 39–89), followed by spleen, omentum and kidney IBs (50%, 95% CI 28–72), mesenteric lymph node IBs and FNAs (45%, 95% CI 23–67), and FNAs of spleen and liver and liver IBs (40%, 95% CI 19–62). In these 20 cats with FIP, FCoVs with S gene mutations were found in every cat in at least one tissue or fluid sample. This highlights the association between mutated S gene and systemic FCoV spread. Examining a combination of different samples increased the probability of finding FCoV with the mutated S gene.
    Keywords Feline coronavirus ; biopsy ; biotypes ; blood serum ; cats ; cerebrospinal fluid ; confidence interval ; feline infectious peritonitis ; genes ; immune response ; immunohistochemistry ; infection ; kidneys ; liver ; lymph nodes ; macrophages ; medicine ; mutation ; nucleotides ; omentum ; prevalence ; sampling ; spleen ; virulence
    Language English
    Dates of publication 2020-08
    Size p. 791-799.
    Publishing place SAGE Publications
    Document type Article
    Note NAL-light
    ZDB-ID 2049047-1
    ISSN 1532-2750 ; 1098-612X
    ISSN (online) 1532-2750
    ISSN 1098-612X
    DOI 10.1177/1098612X19886671
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Feline coronavirus with and without spike gene mutations detected by real-time RT-PCRs in cats with feline infectious peritonitis.

    Emmler, Laura / Felten, Sandra / Matiasek, Kaspar / Balzer, Hans-Joerg / Pantchev, Nikola / Leutenegger, Christian / Hartmann, Katrin

    Journal of feline medicine and surgery

    2019  Volume 22, Issue 8, Page(s) 791–799

    Abstract: Objectives: Feline infectious peritonitis (FIP) emerges when feline coronaviruses (FCoVs) mutate within their host to a highly virulent biotype and the immune response is not able to control the infection. FCoV spike (: Methods: Fine-needle aspirates ...

    Abstract Objectives: Feline infectious peritonitis (FIP) emerges when feline coronaviruses (FCoVs) mutate within their host to a highly virulent biotype and the immune response is not able to control the infection. FCoV spike (
    Methods: Fine-needle aspirates (FNAs) and incisional biopsies (IBs) of popliteal and mesenteric lymph nodes, liver, spleen, omentum and kidneys (each n = 20), EDTA blood (n = 13), buffy coat smears (n = 13), serum (n = 11), effusion (n = 14), cerebrospinal fluid (n = 16), aqueous humour (n = 20) and peritoneal lavage (n = 6) were obtained from 20 cats with FIP diagnosed by immunohistochemistry. Samples were examined by RT-PCR targeting the FCoV
    Results: In 20/20 cats, FCoV with
    Conclusions and relevance: In these 20 cats with FIP, FCoVs with
    MeSH term(s) Animals ; Biopsy, Fine-Needle/veterinary ; Cats ; Coronavirus, Feline/genetics ; Feline Infectious Peritonitis/virology ; Female ; Immunohistochemistry/veterinary ; Lymph Nodes/pathology ; Male ; Mutation ; Prospective Studies ; Real-Time Polymerase Chain Reaction/veterinary ; Reverse Transcriptase Polymerase Chain Reaction/veterinary ; Spike Glycoprotein, Coronavirus/genetics
    Chemical Substances Spike Glycoprotein, Coronavirus ; spike protein, Feline Coronavirus
    Keywords covid19
    Language English
    Publishing date 2019-11-15
    Publishing country England
    Document type Journal Article
    ZDB-ID 2049047-1
    ISSN 1532-2750 ; 1098-612X
    ISSN (online) 1532-2750
    ISSN 1098-612X
    DOI 10.1177/1098612X19886671
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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