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  1. Article ; Online: Fibroblast Migration in 3D is Controlled by Haptotaxis in a Non-muscle Myosin II-Dependent Manner.

    Moreno-Arotzena, O / Borau, C / Movilla, N / Vicente-Manzanares, M / García-Aznar, J M

    Annals of biomedical engineering

    2015  Volume 43, Issue 12, Page(s) 3025–3039

    Abstract: Cell migration in 3D is a key process in many physiological and pathological processes. Although valuable knowledge has been accumulated through analysis of various 2D models, some of these insights are not directly applicable to migration in 3D. In this ...

    Abstract Cell migration in 3D is a key process in many physiological and pathological processes. Although valuable knowledge has been accumulated through analysis of various 2D models, some of these insights are not directly applicable to migration in 3D. In this study, we have confined biomimetic hydrogels within microfluidic platforms in the presence of a chemoattractant (platelet-derived growth factor-BB). We have characterized the migratory responses of human fibroblasts within them, particularly focusing on the role of non-muscle myosin II. Our results indicate a prominent role for myosin II in the integration of chemotactic and haptotactic migratory responses of fibroblasts in 3D confined environments.
    MeSH term(s) Cell Movement/drug effects ; Cell Movement/physiology ; Cells, Cultured ; Fibroblasts/drug effects ; Fibroblasts/physiology ; Humans ; Hydrogels ; Microfluidics ; Myosin Type II/physiology ; Proto-Oncogene Proteins c-sis/pharmacology
    Chemical Substances Hydrogels ; Proto-Oncogene Proteins c-sis ; becaplermin (1B56C968OA) ; Myosin Type II (EC 3.6.1.-)
    Language English
    Publishing date 2015-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 185984-5
    ISSN 1573-9686 ; 0191-5649 ; 0090-6964
    ISSN (online) 1573-9686
    ISSN 0191-5649 ; 0090-6964
    DOI 10.1007/s10439-015-1343-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Characterization of Fibrin and Collagen Gels for Engineering Wound Healing Models.

    Moreno-Arotzena, Oihana / Meier, Johann G / Del Amo, Cristina / García-Aznar, José Manuel

    Materials (Basel, Switzerland)

    2015  Volume 8, Issue 4, Page(s) 1636–1651

    Abstract: Hydrogels are used for ... ...

    Abstract Hydrogels are used for 3D
    Language English
    Publishing date 2015-08-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2487261-1
    ISSN 1996-1944
    ISSN 1996-1944
    DOI 10.3390/ma8041636
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Erratum: Publisher's Note: "Inducing chemotactic and haptotactic cues in microfluidicdevices for three-dimensional in vitro assays" [Biomicrofluidics 8, 064122 (2014)].

    Moreno-Arotzena, O / Mendoza, G / Cóndor, M / Rüberg, T / García-Aznar, J M

    Biomicrofluidics

    2014  Volume 8, Issue 6, Page(s) 69901

    Language English
    Publishing date 2014-12-30
    Publishing country United States
    Document type Published Erratum
    ISSN 1932-1058
    ISSN 1932-1058
    DOI 10.1063/1.4905312
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Inducing chemotactic and haptotactic cues in microfluidic devices for three-dimensional in vitro assays.

    Moreno-Arotzena, O / Mendoza, G / Cóndor, M / Rüberg, T / García-Aznar, J M

    Biomicrofluidics

    2014  Volume 8, Issue 6, Page(s) 64122

    Abstract: Microfluidic devices allow for the production of physiologically relevant cellular microenvironments by including biomimetic hydrogels and generating controlled chemical gradients. During transport, the biomolecules interact in distinct ways with the ... ...

    Abstract Microfluidic devices allow for the production of physiologically relevant cellular microenvironments by including biomimetic hydrogels and generating controlled chemical gradients. During transport, the biomolecules interact in distinct ways with the fibrillar networks: as purely diffusive factors in the soluble fluid or bound to the matrix proteins. These two main mechanisms may regulate distinct cell responses in order to guide their directional migration: caused by the substrate-bound chemoattractant gradient (haptotaxis) or by the gradient established within the soluble fluid (chemotaxis). In this work 3D diffusion experiments, in combination with ELISA assays, are performed using microfluidic platforms in order to quantify the distribution of PDGF-BB and TGF-β1 across collagen and fibrin gels. Furthermore, to gain a deeper understanding of the fundamental processes, the experiments are reproduced by computer simulations based on a reaction-diffusion transport model. This model yields an accurate prediction of the experimental results, confirming that diffusion and binding phenomena are established within the microdevice.
    Language English
    Publishing date 2014-12-11
    Publishing country United States
    Document type Journal Article
    ISSN 1932-1058
    ISSN 1932-1058
    DOI 10.1063/1.4903948
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Image analysis for the quantitative comparison of stress fibers and focal adhesions.

    Elosegui-Artola, Alberto / Jorge-Peñas, Alvaro / Moreno-Arotzena, Oihana / Oregi, Amaia / Lasa, Marta / García-Aznar, José Manuel / De Juan-Pardo, Elena M / Aldabe, Rafael

    PloS one

    2014  Volume 9, Issue 9, Page(s) e107393

    Abstract: Actin stress fibers (SFs) detect and transmit forces to the extracellular matrix through focal adhesions (FAs), and molecules in this pathway determine cellular behavior. Here, we designed two different computational tools to quantify actin SFs and the ... ...

    Abstract Actin stress fibers (SFs) detect and transmit forces to the extracellular matrix through focal adhesions (FAs), and molecules in this pathway determine cellular behavior. Here, we designed two different computational tools to quantify actin SFs and the distribution of actin cytoskeletal proteins within a normalized cellular morphology. Moreover, a systematic cell response comparison between the control cells and those with impaired actin cytoskeleton polymerization was performed to demonstrate the reliability of the tools. Indeed, a variety of proteins that were present within the string beginning at the focal adhesions (vinculin) up to the actin SFs contraction (non-muscle myosin II (NMMII)) were analyzed. Finally, the software used allows for the quantification of the SFs based on the relative positions of FAs. Therefore, it provides a better insight into the cell mechanics and broadens the knowledge of the nature of SFs.
    MeSH term(s) Cell Shape ; Focal Adhesions/metabolism ; Focal Adhesions/ultrastructure ; HeLa Cells ; Humans ; Imaging, Three-Dimensional ; Microscopy, Fluorescence ; Protein Transport ; Stress Fibers/metabolism ; Stress Fibers/ultrastructure
    Language English
    Publishing date 2014-09-30
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0107393
    Database MEDical Literature Analysis and Retrieval System OnLINE

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