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  1. Article: Preventing scattering of Tetranychus urticae in Rosa hybrida through dsCOPB2 expression

    Lee, Su Young / Shin, Ju Young / Kwon, Deok Ho / Xu, Junping / Kim, Jeong Ho / Ahn, Chang Ho / Jang, Seonghoe / Kwon, O Hyeon / Lee, Hye Jin / Kim, Won Hee

    Scientia horticulturae. 2022 July 27, v. 301

    2022  

    Abstract: To obtain rose plants resistant to Tetranychus urticae, which is one of the major pests of roses grown in a plastic house or greenhouse to be harvested as cut-flowers, we introduced the double-stranded RNA of the coatomer protein complex (dsCOPB2) from T. ...

    Abstract To obtain rose plants resistant to Tetranychus urticae, which is one of the major pests of roses grown in a plastic house or greenhouse to be harvested as cut-flowers, we introduced the double-stranded RNA of the coatomer protein complex (dsCOPB2) from T. urticae (inserted into the pPZP200-Bar vector) to somatic embryos or embryogenic calluses of a rose breeding line (KR056006), using an Agrobacterium-mediated transformation method. It took 11 to 41 months to obtain eight plants, which were regenerated from the somatic embryos or embryogenic calluses through a selection procedure that uses phosphinothricin acetyltransferase (ppt; 2 mg L ⁻ ¹) after co-cultivation. Validation of the transgene in pseudo-transgenic plants was verified via genomic polymerase chain reaction (PCR) and Southern Blot analyses. It was confirmed that one to six copies of the transgene were transferred to the eight dsCOPB2-transgenic lines; the levels of the transgene were analyzed via real-time quantitative PCR (RT-qPCR). In the resistance test of one transgenic line (KR056006-COPB2–13) against T. urticae, the pest showed a high mortality rate of approximately (∼89%), and egg-laying was relatively decreased by approximately 88%, compared to those for a non-transgenic (NT) rose line, KR056006. This suggests that the transgenic rose plants with high accumulated amounts of dsCOPB2 have a negative effect on the survival of T. urticae. It is expected that these results will accelerate the development of pest-resistant cultivars and may replace conventional pest control methods in general.
    Keywords Rosa hybr. ; Southern blotting ; Tetranychus urticae ; coculture ; cultivars ; cut flowers ; double-stranded RNA ; genetically modified organisms ; genomics ; greenhouses ; mortality ; oviposition ; pest control ; pest resistance ; pests ; phosphinothricin acetyltransferase ; quantitative polymerase chain reaction ; transgenes
    Language English
    Dates of publication 2022-0727
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 185557-8
    ISSN 0304-4238
    ISSN 0304-4238
    DOI 10.1016/j.scienta.2022.111113
    Database NAL-Catalogue (AGRICOLA)

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  2. Article: Comparative Analysis of Volatile Compounds of Gamma-Irradiated Mutants of Rose (Rosa hybrida)

    Ryu, Jaihyunk / Lyu, Jae Il / Kim, Dong-Gun / Kim, Jung-Min / Jo, Yeong Deuk / Kang, Si-Yong / Kim, Jin-Baek / Ahn, Joon-Woo / Kim, Sang Hoon

    Plants. 2020 Sept. 17, v. 9, no. 9

    2020  

    Abstract: Roses are one of the most important floricultural crops, and their essential oils have long been used for cosmetics and aromatherapy. We investigated the volatile compound compositions of 12 flower-color mutant variants and their original cultivars. ... ...

    Abstract Roses are one of the most important floricultural crops, and their essential oils have long been used for cosmetics and aromatherapy. We investigated the volatile compound compositions of 12 flower-color mutant variants and their original cultivars. Twelve rose mutant genotypes were developed by treatment with 70 Gy of ⁶⁰Co gamma irradiation of six commercial rose cultivars. Essential oils from the flowers of the 18 genotypes were analyzed by gas chromatography–mass spectrometry. Seventy-seven volatile compounds were detected, which were categorized into six classes: Aliphatic hydrocarbons, aliphatic alcohols, aliphatic ester, aromatic compounds, terpene alcohols, and others. Aliphatic (hydrocarbons, alcohols, and esters) compounds were abundant categories in all rose flowers. The CR-S2 mutant had the highest terpene alcohols and oil content. Three (CR-S1, CR-S3, and CR-S4) mutant genotypes showed higher ester contents than their original cultivar. Nonacosane, 2-methylhexacosane, and 2-methyltricosane were major volatile compounds among all genotypes. Hierarchical cluster analysis (HCA) of the rose genotypes gave four groups according to grouping among the 77 volatile compounds. In addition, the principal component analysis (PCA) model was successfully applied to distinguish most attractive rose lines. These findings will be useful for the selection of rose genotypes with improved volatile compounds.
    Keywords Rosa hybr. ; alcohols ; aromatic compounds ; cluster analysis ; cobalt ; cultivars ; essential oils ; esters ; floriculture crops ; flower color ; flowers ; gamma radiation ; gas chromatography-mass spectrometry ; genotype ; hybrids ; hydrocarbons ; lipid content ; models ; mutants ; principal component analysis ; radionuclides ; volatile compounds
    Language English
    Dates of publication 2020-0917
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants9091221
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: Clinical Evaluation of a New-Formula Shampoo for Scalp Seborrheic Dermatitis Containing Extract of Rosa centifolia Petals and Epigallocatechin Gallate: A Randomized, Double-Blind, Controlled Study.

    Kim, Yu Ri / Kim, Jeong-Hwan / Shin, Hong-Ju / Choe, Yong Beom / Ahn, Kyu Joong / Lee, Yang Won

    Annals of dermatology

    2014  Volume 26, Issue 6, Page(s) 733–738

    Abstract: ... of Rosa centifolia petals and epigallocatechin gallate (EGCG)-that exert antioxidative, anti-inflammatory ...

    Abstract Background: Scalp seborrheic dermatitis is a chronic type of inflammatory dermatosis that is associated with sebum secretion and proliferation of Malassezia species. Ketoconazole or zinc-pyrithione shampoos are common treatments for scalp seborrheic dermatitis. However, shampoos comprising different compounds are required to provide patients with a wider range of treatment options.
    Objective: This study was designed to evaluate a new-formula shampoo that contains natural ingredients-including extract of Rosa centifolia petals and epigallocatechin gallate (EGCG)-that exert antioxidative, anti-inflammatory, and sebum secretion inhibitory effects, and antifungal agents for the treatment of scalp seborrheic dermatitis.
    Methods: Seventy-five patients were randomized into three treatment groups; new-formula shampoo, 2% ketoconazole shampoo, and 1% zinc- pyrithione shampoo. The clinical severity scores and sebum levels were assessed by the same dermatologists at baseline (week 0), and at 2 and 4 weeks after using the shampoo. User satisfaction and irritation were also assessed with the aid of a questionnaire.
    Results: The efficacy of the new-formula shampoo was comparable to that of both the 1% zinc-pyrithione shampoo and the 2% ketoconazole shampoo. Furthermore, it was found to provide a more rapid response than the 1% zinc-pyrithione shampoo for mild erythema lesions and was associated with greater user satisfaction compared with the 2% ketoconazole shampoo. However, the new-formula shampoo did not exhibit the previously reported sebum inhibitory effect.
    Conclusion: Extract of R. centifolia petals or EGCG could be useful ingredients in the treatment of scalp seborrheic dermatitis.
    Language English
    Publishing date 2014-11-26
    Publishing country Korea (South)
    Document type Journal Article
    ZDB-ID 1012662-4
    ISSN 2005-3894 ; 1013-9087
    ISSN (online) 2005-3894
    ISSN 1013-9087
    DOI 10.5021/ad.2014.26.6.733
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Effect of LEDs (Light Emitting Diodes) Irradiation on Growth and Mineral Absorption of Lettuce (Lactuca sativa L. 'Lollo Rosa')

    Shin, Y.S., Gyeongsangbuk-do Agricultural Research and Extension Service, Daegu, Republic of Korea / Lee, M.J., Gyeongsangbuk-do Agricultural Research and Extension Service, Daegu, Republic of Korea / Lee, E.S., Gyeongsangbuk-do Agricultural Research and Extension Service, Daegu, Republic of Korea / Ahn, J.H., Gyeongsangbuk-do Agricultural Research and Extension Service, Daegu, Republic of Korea / Lim, J.H., Gyeongsangbuk-do Agricultural Research and Extension Service, Daegu, Republic of Korea / Kim, H.J., Ecotech Co., Ltd., Chilgok, Republic of Korea / Park, H.W., KAST Eco-friendly Farming Techniques Co., Ltd., Gumi, Republic of Korea / Um, Y.G., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea / Park, S.D., Gyeongsangbuk-do Agricultural Research and Extension Service, Daegu, Republic of Korea / Chai, J.H., Gyeongsangbuk-do Agricultural Research and Extension Service, Daegu, Republic of Korea

    Journal of Bio-Environment Control

    (Sep 2012)  Volume v. 21, Issue (3), Page(s) p. 180–185

    Abstract: ... irradiation in relation to early growth and inorganic elements in leaf lettuce (Lactuca sativa L. 'Rollo Rosa' ...

    Abstract The objective of this study was carried out to elucidate the effect of LEDs (light emitting diodes) irradiation in relation to early growth and inorganic elements in leaf lettuce (Lactuca sativa L. 'Rollo Rosa'). In morphological changes of leaves, shoot elongation and hypocotyl length showed poor growth in red light irradiation, while the red + blue light irradiation induced shorter plant height and much greater leaf numbers resulting in increased fresh weight. In change of the Hunter's color and SPAD values, lettuce seedlings grown under in red + blue and fluorescent light irradiation had a higher a* value, otherwise SPAD values were not changed in these light irradiations. Interestingly, relative chlorophyll contents showed 1.8 times increased redness in the treatment of red + blue light irradiation. Inorganic element (N, Ca, Mg, Mn, and Fe) and ascorbic acid contents were increased in lettuce plants grown under LEDs light irradiation compared to those of lettuce grown under the fluorescent light which showed higher P and Mn contents. In conclusion, it is considered that red + blue light irradiation which stimulates growth and higher nutrient uptake in leaf lettuce could be employed in containers equipped with LEDs.
    Keywords MINERALS ; GROWTH ; CROISSANCE ; MINERAUX ; CRECIMIENTO ; MINERALES
    Language English
    Document type Article
    ISSN 1229-4675
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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  5. Article: Rose (Rosa hybrida L.) EST-derived microsatellite markers and their transferability to strawberry (Fragaria spp.)

    Park, Young Hoon / Ahn, Seong Gyu / Choi, Young Mi / Oh, Hye Jeong / Ahn, Dong Chun / Kim, Jin Gi / Kang, Jum Soon / Choi, Young Whan / Jeong, Byoung Ryong

    Scientia horticulturae. 2010 July 26, v. 125, no. 4

    2010  

    Abstract: ... ESTs) harboring simple sequence repeats (SSRs) from several Rosaceae genera, including Rosa (rose ... Primer pairs flanking SSR were designed for 312 unique Rosa ESTs based on GDR database. Eight rose (Rosa ...

    Abstract The ‘Genome database for Rosaceae (GDR)' provides a large collection of expressed sequence tags (ESTs) harboring simple sequence repeats (SSRs) from several Rosaceae genera, including Rosa (rose). Primer pairs flanking SSR were designed for 312 unique Rosa ESTs based on GDR database. Eight rose (Rosa hybrida L.) genotypes were tested for PCR amplification, and 287 (92%) of the primer pairs generated allele-specific PCR bands that were readily scored. From 183 (63.7%) primer pairs that evidenced polymorphic alleles among the eight rose cultivars, 20 pairs evidencing EST sequence homology to known gene functions and high levels of polymorphism were selected and utilized for DNA fingerprinting and genetic diversity assessments of 47 rose hybrids. A total of 202 polymorphic bands were scored and generated unique fingerprints for each rose hybrid. The Nei-Li genetic similarity coefficients among 1081 pair-wise comparisons of 47 cultivars exhibited a broad range of genetic variations from 0.30 (‘Grand King' and ‘Carnival') to 0.99 (‘First Red' and ‘Red Champ'). UPGMA cluster analysis divided 47 hybrids into five major groups and two sub-groups. The cross-species transferability of 273 EST-SSR primer pairs was evaluated using four genotypes of the strawberry, a genus member of the Rosaceae family. PCRs on the DNA samples of strawberry were successful for 165 primer pairs; among these, 123 pairs amplified 243 polymorphic bands. As surrogates of the marker transfer, the phenetic relationship among the four strawberry genotypes was evaluated. Genetic similarity coefficients varied from 0.78 (‘Maehyang' and ‘Janghyee') to 0.64 (‘Janghyee' and ‘Pragana'). The results of cluster analysis showed that the three octaploid strawberry cultivars were quite similar, whereas the diploid ‘Pragana' was related distantly at the genomic DNA level. The EST-SSR markers developed in the present study can be efficiently utilized for genetic diversity studies in Rosaceae.
    Keywords Rosa ; cut flowers ; Fragaria ; strawberries ; genetic markers ; microsatellite repeats ; expressed sequence tags ; DNA primers ; polymerase chain reaction ; genome ; DNA libraries ; sequence analysis ; genetic polymorphism ; alleles ; DNA fingerprinting ; genetic distance ; genotype ; phylogeny
    Language English
    Dates of publication 2010-0726
    Size p. 733-739.
    Publishing place [Amsterdam; New York, NY]: Elsevier Science
    Document type Article
    ZDB-ID 185557-8
    ISSN 0304-4238
    ISSN 0304-4238
    DOI 10.1016/j.scienta.2010.05.012
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Mental Health-Related Outpatient Visits Among Adolescents and Young Adults, 2006-2019.

    Ahn-Horst, Rosa Y / Bourgeois, Florence T

    JAMA network open

    2024  Volume 7, Issue 3, Page(s) e241468

    Abstract: Importance: Concerns over the mental health of young people have been increasing over the past decade, especially with the rise in mental health burden seen during the COVID-19 pandemic. Examining trends in mental health-related outpatient visits ... ...

    Abstract Importance: Concerns over the mental health of young people have been increasing over the past decade, especially with the rise in mental health burden seen during the COVID-19 pandemic. Examining trends in mental health-related outpatient visits provides critical information to elucidate contributing factors, identify vulnerable populations, and inform strategies to address the mental health crisis.
    Objective: To examine characteristics and trends in mental health-related outpatient visits and psychotropic medication use among US adolescents and young adults.
    Design, setting, and participants: A retrospective cross-sectional analysis of nationally representative data from the National Ambulatory Medical Care Survey, an annual probability sample survey, was conducted from January 2006 to December 2019. Participants included adolescents (age 12-17 years) and young adults (age 18-24 years) with office-based outpatient visits in the US. Data were analyzed from March 1, 2023, to September 15, 2023.
    Main outcomes and measures: Mental health-related outpatient visits were identified based on established sets of diagnostic codes for psychiatric disorders. Temporal trends in the annual proportion of mental health-related outpatient visits were assessed, including visits associated with use of psychotropic medications. Analyses were stratified by age and sex.
    Results: From 2006 to 2019, there were an estimated 1.1 billion outpatient visits by adolescents and young adults, of which 145.0 million (13.1%) were associated with a mental health condition (mean [SD] age, 18.4 [3.5] years; 74.0 million females [51.0%]). Mental health-related diagnoses were more prevalent among visits by male (16.8%) compared with female (10.9%) patients (P < .001). This difference was most pronounced among young adults, with 20.1% of visits associated with a psychiatric diagnosis among males vs 10.1% among females (P < .001). The proportion of mental health-related visits nearly doubled, from 8.9% in 2006 to 16.9% in 2019 (P < .001). Among all outpatient visits, 17.2% were associated with the prescription of at least 1 psychotropic medication, with significant increases from 12.8% to 22.4% by 2019 (P < .001).
    Conclusions and relevance: In this cross-sectional study, there were substantial increases in mental health-related outpatient visits and use of psychotropic medications, with greater overall burden among male patients. These findings provide a baseline for understanding post-pandemic shifts and suggest that current treatment and prevention strategies will need to address preexisting psychiatric needs in addition to the effects of the COVID-19 pandemic.
    MeSH term(s) Humans ; Adolescent ; Female ; Male ; Young Adult ; Child ; Adult ; Mental Health ; Cross-Sectional Studies ; Outpatients ; Pandemics ; Retrospective Studies ; COVID-19/epidemiology
    Language English
    Publishing date 2024-03-04
    Publishing country United States
    Document type Journal Article
    ISSN 2574-3805
    ISSN (online) 2574-3805
    DOI 10.1001/jamanetworkopen.2024.1468
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Unpublished trials of alprazolam XR and their influence on its apparent efficacy for panic disorder.

    Ahn-Horst, Rosa Y / Turner, Erick H

    Psychological medicine

    2023  Volume 54, Issue 5, Page(s) 1026–1033

    Abstract: Objective: To test for publication bias with alprazolam, the most widely prescribed benzodiazepine, by comparing its efficacy for panic disorder using trial results from (1) the published literature and (2) the US Food and Drug Administration (FDA).: ... ...

    Abstract Objective: To test for publication bias with alprazolam, the most widely prescribed benzodiazepine, by comparing its efficacy for panic disorder using trial results from (1) the published literature and (2) the US Food and Drug Administration (FDA).
    Methods: From FDA reviews, we included data from all phase 2/3 efficacy trials of alprazolam extended-release (Xanax XR) for the treatment of panic disorder. A search for matching publications was performed using PubMed and Google Scholar. Publication bias was examined by comparing: (1) overall trial results (positive or not) according to the FDA
    Results: The FDA review showed that five trials were conducted, only one of which (20%) was positive. Of the four not-positive trials, two were published conveying a positive outcome; the other two were not published. Thus, according to the published literature, three trials were conducted and all (100%) were positive. Alprazolam's effect size calculated using FDA data was 0.33 (CI
    Conclusions: Publication bias substantially inflates the apparent efficacy of alprazolam XR.
    MeSH term(s) Humans ; Alprazolam/pharmacology ; Alprazolam/therapeutic use ; Panic Disorder/drug therapy ; Benzodiazepines/therapeutic use ; Publication Bias
    Chemical Substances Alprazolam (YU55MQ3IZY) ; Benzodiazepines (12794-10-4)
    Language English
    Publishing date 2023-10-19
    Publishing country England
    Document type Journal Article
    ZDB-ID 217420-0
    ISSN 1469-8978 ; 0033-2917
    ISSN (online) 1469-8978
    ISSN 0033-2917
    DOI 10.1017/S0033291723002830
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Correction to: Do Limitations in the Design of PARADIGM-HF Justify the Slow Real World Uptake of Sacubitril/Valsartan (Entresto)?

    Ahn, Rosa / Prasad, Vinay

    Cardiovascular drugs and therapy

    2018  Volume 32, Issue 6, Page(s) 637

    Abstract: The original version of this article unfortunately contained a mistake. There was a typo in the second sentence of the paragraph under the section Was the Dose of Drugs Fair?; "320 mg twice a day" should read "160mg twice a day". The corrected paragraph ... ...

    Abstract The original version of this article unfortunately contained a mistake. There was a typo in the second sentence of the paragraph under the section Was the Dose of Drugs Fair?; "320 mg twice a day" should read "160mg twice a day". The corrected paragraph is shown below.
    Language English
    Publishing date 2018-10-01
    Publishing country United States
    Document type Journal Article ; Published Erratum
    ZDB-ID 639068-7
    ISSN 1573-7241 ; 0920-3206
    ISSN (online) 1573-7241
    ISSN 0920-3206
    DOI 10.1007/s10557-018-6834-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Characteristics of Public Comments Submitted to State Health Technology Assessment Programs in Oregon and Washington.

    Ahn, Rosa / Herrera-Perez, Diana / Prasad, Vinay

    JAMA internal medicine

    2019  Volume 180, Issue 2, Page(s) 329–331

    MeSH term(s) Community Participation ; Conflict of Interest ; Humans ; Insurance Coverage ; Medicaid ; Oregon ; Technology Assessment, Biomedical ; United States ; Washington
    Language English
    Publishing date 2019-12-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2699338-7
    ISSN 2168-6114 ; 2168-6106
    ISSN (online) 2168-6114
    ISSN 2168-6106
    DOI 10.1001/jamainternmed.2019.5145
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Do Limitations in the Design of PARADIGM-HF Justify the Slow Real World Uptake of Sacubitril/Valsartan (Entresto)?

    Ahn, Rosa / Prasad, Vinay

    Cardiovascular drugs and therapy

    2018  Volume 32, Issue 6, Page(s) 633–635

    MeSH term(s) Aminobutyrates/adverse effects ; Aminobutyrates/therapeutic use ; Angiotensin Receptor Antagonists/adverse effects ; Angiotensin Receptor Antagonists/therapeutic use ; Drug Approval ; Drug Combinations ; Evidence-Based Medicine ; Heart Failure/diagnosis ; Heart Failure/drug therapy ; Heart Failure/mortality ; Heart Failure/physiopathology ; Humans ; Neprilysin/antagonists & inhibitors ; Practice Patterns, Physicians'/trends ; Protease Inhibitors/adverse effects ; Protease Inhibitors/therapeutic use ; Randomized Controlled Trials as Topic/methods ; Research Design ; Risk Factors ; Tetrazoles/adverse effects ; Tetrazoles/therapeutic use ; Treatment Outcome ; United States ; United States Food and Drug Administration
    Chemical Substances Aminobutyrates ; Angiotensin Receptor Antagonists ; Drug Combinations ; Protease Inhibitors ; Tetrazoles ; Neprilysin (EC 3.4.24.11) ; sacubitril and valsartan sodium hydrate drug combination (WB8FT61183)
    Language English
    Publishing date 2018-09-14
    Publishing country United States
    Document type Letter
    ZDB-ID 639068-7
    ISSN 1573-7241 ; 0920-3206
    ISSN (online) 1573-7241
    ISSN 0920-3206
    DOI 10.1007/s10557-018-6830-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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