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Article ; Online: PDGF Receptors and Signaling Are Required for 3D-Structure Formation and Differentiation of Human iPSC-Derived Hepatic Spheroids.

Tsuzuki, Syusaku / Yamaguchi, Tomoyuki / Okumura, Takashi / Kasai, Toshiharu / Ueno, Yasuharu / Taniguchi, Hideki

International journal of molecular sciences

2023 Volume 24, Issue 8

Abstract: Human iPSC-derived liver organoids (LO) or hepatic spheroids (HS) have attracted widespread interest, and the numerous studies on them have recently provided various production protocols. However, the mechanism by which the 3D structures of LO and HS are ...

Abstract	Human iPSC-derived liver organoids (LO) or hepatic spheroids (HS) have attracted widespread interest, and the numerous studies on them have recently provided various production protocols. However, the mechanism by which the 3D structures of LO and HS are formed from the 2D-cultured cells and the mechanism of the LO and HS maturation remain largely unknown. In this study, we demonstrate that
MeSH term(s)	Humans ; Mice ; Animals ; Induced Pluripotent Stem Cells ; Cell Culture Techniques/methods ; Liver ; Hepatocytes ; Cell Differentiation ; Receptor, Platelet-Derived Growth Factor alpha/genetics ; Spheroids, Cellular
Chemical Substances	Receptor, Platelet-Derived Growth Factor alpha (EC 2.7.10.1)
Language	English
Publishing date	2023-04-11
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms24087075
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Article ; Online: Present state of intestinal transplantation in Japan.

Ueno, Takehisa / Wada, Motoshi / Ogawa, Eri / Matsuura, Toshiharu / Yamada, Yohei / Sakamoto, Seisuke / Okuyama, Hiroomi

Pediatric surgery international

2023 Volume 39, Issue 1, Page(s) 276

Abstract: Introduction: Intestinal transplantation (ITx) is the ultimate treatment for intestinal failure (IF). In Japan, most cases of IF are a result of pediatric disease, including secondary or congenital intestinal disease or allied disorders of Hirschsprung' ... ...

Abstract	Introduction: Intestinal transplantation (ITx) is the ultimate treatment for intestinal failure (IF). In Japan, most cases of IF are a result of pediatric disease, including secondary or congenital intestinal disease or allied disorders of Hirschsprung's disease. Here, we report the results of the Japanese ITx registry. Methods: A web-based survey form was completed. We investigated the number, age, sex, indication, surgical procedure, immunosuppressants, postoperative course, and the effects of transplantation in patients who underwent cadaveric or living-donor ITx. Results: By the end of 2022, 42 cases of ITx have been performed in 38 patients in Japan. The donor sources included cadavers (29 cases) and living donors (13 cases). The surgical method was isolated ITx (N = 40) and combined liver and ITx (n = 2). Survival rates were 92%, 73%, and 59% at 1 year, 5 years, and 10 years, respectively. Ninety percent of patients completely discontinued parenteral nutrition. Approximately 80% of the patients had a performance status of 1 or less, indicating that the QOL of patients after ITx was extremely good. Conclusion: The results of ITx are acceptable to treat IF patients and the QOL after transplantation is also good.
MeSH term(s)	Child ; Humans ; Japan ; Quality of Life ; Short Bowel Syndrome/surgery ; Retrospective Studies ; Intestines ; Intestinal Diseases/surgery ; Living Donors
Language	English
Publishing date	2023-09-27
Publishing country	Germany
Document type	Journal Article
ZDB-ID	632773-4
ISSN	1437-9813 ; 0179-0358
ISSN (online)	1437-9813
ISSN	0179-0358
DOI	10.1007/s00383-023-05552-5
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Zs.A 2138: Show issues

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Article ; Online: Biopsy-proven Streptococcus suis-associated Infectious Glomerulonephritis.

Kajitani, Hideto / Nishiwaki, Hiroki / Ueno, Toshiharu / Koiwa, Fumihiko / Iwasaki, Shigeki / Hirade, Satoshi

Internal medicine (Tokyo, Japan)

2021 Volume 61, Issue 8, Page(s) 1201–1204

Abstract: A 64-year-old Japanese man who worked at a butcher shop was hospitalized for a fever, headache, and deafness. We diagnosed him with sepsis and meningitis caused by Streptococcus suis infection. The patient's renal function declined rapidly, and ... ...

Abstract	A 64-year-old Japanese man who worked at a butcher shop was hospitalized for a fever, headache, and deafness. We diagnosed him with sepsis and meningitis caused by Streptococcus suis infection. The patient's renal function declined rapidly, and hemodialysis was performed temporarily. A renal biopsy was performed, and the renal function tended to improve with antimicrobial therapy. This case seemed rather similar to one of staphylococcal-associated nephritis in that it showed mesangial proliferative nephritis with immunoglobulin A deposition, even though the nephritis was caused by streptococci. Similarly, intramembranous electron-dense deposits were characteristic findings. We present new findings of an in vivo renal biopsy in a case of S. suis-associated glomerulonephritis.
MeSH term(s)	Biopsy ; Female ; Glomerulonephritis/complications ; Glomerulonephritis/pathology ; Humans ; Male ; Middle Aged ; Nephritis ; Streptococcus suis
Language	English
Publishing date	2021-09-25
Publishing country	Japan
Document type	Case Reports ; Journal Article
ZDB-ID	32371-8
ISSN	1349-7235 ; 0021-5120 ; 0918-2918
ISSN (online)	1349-7235
ISSN	0021-5120 ; 0918-2918
DOI	10.2169/internalmedicine.7823-21
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Article: Genetic and morphological approach to reappraising species validity in two different Anagyrus wasps (Hymenoptera: Encyrtidae) attracted by cyclolavandulyl butyrate

Sugawara, Yuma / Mita, Toshiharu / Tabata, Jun / Ueno, Takatoshi

Entomological science. 2020 June, v. 23, no. 2

2020

Abstract: Members of the genus Anagyrus are primary endoparasitoids of mealybugs and thus include potentially important biological control agents of mealybug pests. We have previously discovered that an analogous compound of a mealybug pheromone, cyclolavandulyl ... ...

Abstract	Members of the genus Anagyrus are primary endoparasitoids of mealybugs and thus include potentially important biological control agents of mealybug pests. We have previously discovered that an analogous compound of a mealybug pheromone, cyclolavandulyl butyrate (CLB), strongly attracts Anagyrus sawadai Ishii and can enhance the foraging activity of this wasp in CLB‐treated orchards. In the present study, we found two groups of Anagyrus parasitoids that differed in color were attracted to CLB. One was A. sawadai and the other type was morphologically identified as A. subalbipes Ishii, which had been considered to be synonymous with A. sawadai. We suspected these two color populations of Anagyrus must be independent species because of their different emergence patterns in the field. Our morphological and molecular analyses supported this idea. We confirmed morphologically diagnostic features to distinguish the two species. Furthermore, molecular phylogenetic analysis based on COI sequences revealed that A. sawadai and A. subalbipes were placed in entirely different clusters. These findings not only offered new insight into the taxonomy and phylogeny of Anagyrus spp. but also provided critical knowledge for the use of these indigenous natural enemies for biological control of mealybugs in agricultural fields.
Keywords	Anagyrus ; Pseudococcidae ; biological control ; biological control agents ; butyrates ; color ; endoparasitoids ; foraging ; knowledge ; natural enemies ; orchards ; pheromones ; phylogeny ; population ; taxonomy ; validity ; wasps
Language	English
Dates of publication	2020-06
Size	p. 152-164.
Publishing place	John Wiley & Sons Australia, Ltd
Document type	Article
Note	NAL-light ; JOURNAL ARTICLE
ZDB-ID	2105599-3
ISSN	1479-8298 ; 1343-8786
ISSN (online)	1479-8298
ISSN	1343-8786
DOI	10.1111/ens.12409
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: The effect of the Kasuya CKD network on prevention of the progression of chronic kidney disease: successful collaboration of a public health service, primary care physicians and nephrologists-community based cohort study.

Katafuchi, Ritsuko / Tanaka, Shigeru / Matsuo, Takayuki / Tamai, Osamu / Yoshimine, Kazuhiro / Yano, Kazutoshi / Ueno, Kiichiro / Shimohashi, Naoya / Ninomiya, Toshiharu

Clinical and experimental nephrology

2022 Volume 27, Issue 1, Page(s) 32–43

Abstract: Background: In 2012, we established a CKD network in collaboration with the public health service, primary care physicians, and nephrologists in the Kasuya area. The aim of this study was to clarify if our CKD network was effective in preventing CKD ... ...

Abstract	Background: In 2012, we established a CKD network in collaboration with the public health service, primary care physicians, and nephrologists in the Kasuya area. The aim of this study was to clarify if our CKD network was effective in preventing CKD progression. Methods: 1591 subjects, who had CKD in health checks in 2012 were included in this study. The slope of estimated glomerular filtration rate (eGFR) was compared before and after 2012. Parameters at the first health check visit before 2012, visit in 2012, and the last visit after 2012, were compared. Paired t test, analysis of variance for repeated measurements, and the Friedman test were used for the analysis. Results: Mean age was 65 years. There were 781 men and 810 women. Mean eGFR was 59 ml/min/1.73 m Conclusion: The Kasuya CKD network may be effective in preventing CKD progression.
MeSH term(s)	Aged ; Female ; Humans ; Male ; Cohort Studies ; Community Health Services ; Disease Progression ; Glomerular Filtration Rate ; Nephrologists ; Physicians, Primary Care ; Renal Insufficiency, Chronic/diagnosis ; Renal Insufficiency, Chronic/prevention & control ; Renal Insufficiency, Chronic/metabolism
Language	English
Publishing date	2022-10-07
Publishing country	Japan
Document type	Journal Article
ZDB-ID	1338768-6
ISSN	1437-7799 ; 1342-1751
ISSN (online)	1437-7799
ISSN	1342-1751
DOI	10.1007/s10157-022-02267-0
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Zs.A 4922: Show issues

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Article ; Online: Serum high-sensitivity C-reactive protein and dementia in a community-dwelling Japanese older population (JPSC-AD).

Scientific reports

2024 Volume 14, Issue 1, Page(s) 7374

Abstract: In recent years, the association between neuroinflammatory markers and dementia, especially Alzheimer's disease (AD), has attracted much attention. However, the evidence for the relationship between serum-hs-CRP and dementia including AD are inconsistent. ...

Abstract	In recent years, the association between neuroinflammatory markers and dementia, especially Alzheimer's disease (AD), has attracted much attention. However, the evidence for the relationship between serum-hs-CRP and dementia including AD are inconsistent. Therefore, the relationships of serum high-sensitivity CRP (hs-CRP) with dementia including AD and with regions of interest of brain MRI were investigated. A total of 11,957 community residents aged 65 years or older were recruited in eight sites in Japan (JPSC-AD Study). After applying exclusion criteria, 10,085 participants who underwent blood tests and health-related examinations were analyzed. Then, serum hs-CRP levels were classified according to clinical cutoff values, and odds ratios for the presence of all-cause dementia and its subtypes were calculated for each serum hs-CRP level. In addition, the association between serum hs-CRP and brain volume regions of interest was also examined using analysis of covariance with data from 8614 individuals in the same cohort who underwent brain MRI. After multivariable adjustment, the odds ratios (ORs) for all-cause dementia were 1.04 (95% confidence interval [CI] 0.76-1.43), 1.68 (95%CI 1.08-2.61), and 1.51 (95%CI 1.08-2.11) for 1.0-1.9 mg/L, 2.0-2.9 mg/L, and ≥ 3.0 mg/L, respectively, compared to < 1.0 mg/L, and those for AD were 0.72 (95%CI 0.48-1.08), 1.76 (95%CI 1.08-2.89), and 1.61 (95%CI 1.11-2.35), for 1.0-1.9 mg/L, 2.0-2.9 mg/L, and ≥ 3.0 mg/L, respectively, compared to < 1.0 mg/L. Multivariable-adjusted ORs for all-cause dementia and for AD prevalence increased significantly with increasing serum hs-CRP levels (p for trend < 0.001 and p = 0.001, respectively). In addition, the multivariable-adjusted temporal cortex volume/estimated total intracranial volume ratio decreased significantly with increasing serum hs-CRP levels (< 1.0 mg/L 4.28%, 1.0-1.9 mg/L 4.27%, 2.0-2.9 mg/L 4.29%, ≥ 3.0 mg/L 4.21%; p for trend = 0.004). This study's results suggest that elevated serum hs-CRP levels are associated with greater risk of presence of dementia, especially AD, and of temporal cortex atrophy in a community-dwelling Japanese older population.
MeSH term(s)	Humans ; C-Reactive Protein/metabolism ; Alzheimer Disease/epidemiology ; Japan/epidemiology ; Independent Living ; Risk Factors ; Biomarkers
Chemical Substances	C-Reactive Protein (9007-41-4) ; Biomarkers
Language	English
Publishing date	2024-03-28
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-57922-1
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Article ; Online: Comparison of Lipoprotein Cholesterol Levels in Golden Syrian Hamster Administrated trans-Octadecenoic Acid Positional Isomers.

Nagao, Koji / Ueno, Junya / Yoshinaga, Kazuaki / Nagai, Toshiharu / Mizobe, Hoyo / Yoshida, Akihiko / Beppu, Fumiaki / Tanaka, Seiya / Gotoh, Naohiro

Journal of oleo science

2022 Volume 71, Issue 4, Page(s) 609–618

Abstract: We previously conducted a study using HepG2 cells to compare the effect on the secreted apolipoprotein B-100 and apolipoprotein A-1 ratio (B-100/A-1) corresponding to the ratio of low-density to high-density lipoprotein cholesterol (LDL/HDL) among 13 ... ...

Abstract	We previously conducted a study using HepG2 cells to compare the effect on the secreted apolipoprotein B-100 and apolipoprotein A-1 ratio (B-100/A-1) corresponding to the ratio of low-density to high-density lipoprotein cholesterol (LDL/HDL) among 13 types of trans-octadecenoic acid (t-18:1) positional isomers. The results revealed that trans-5-18:1 (t5) significantly increased B-100/A-1. In this study, 1% of t5 in the diet, corresponding to 2.08 energy%, was administrated golden Syrian hamsters for 4 weeks to reveal the effects on lipid profiles, including LDL/HDL, by comparing cis-9-octadecenoic acid (OA, oleic acid), trans-9-octadecenoic acid (EA), trans-11-octadecenoic acid (VA), and trans-9,trans-12- octadecadienoic acid (TT). LDL/HDL was not significantly different among the groups. However, the cholesterol concentration of medium very low-density lipoprotein (VLDL) was significantly lower in the TT diet than in the OA and t5 diets. The cholesterol concentration of small VLDL was significantly lower in the TT diet than in the OA, t5, and EA diets. The cholesterol concentration of large LDL was significantly lower in the TT diet than in the t5 and EA diets. However, no significant difference was detected between the TT and OA diets. In contrast, the cholesterol concentration of very small HDL was significantly higher in the TT diet than in the t5 diet. These results would support that lipid metabolism is affected by the structure of TFA in animals. However, t5-18:1 did not significantly change any lipid profile compared to OA existing in nature, and the previous result from the cell experiment showing that t5 increased B-100/A-1 (LDL/HDL) was not confirmed in this animal experiment.
MeSH term(s)	Animals ; Cholesterol/metabolism ; Cholesterol, HDL ; Cricetinae ; Dietary Fats/pharmacology ; Lipoproteins/metabolism ; Mesocricetus ; Stearic Acids ; Triglycerides
Chemical Substances	5-octadecenoic acid ; Cholesterol, HDL ; Dietary Fats ; Lipoproteins ; Stearic Acids ; Triglycerides ; lipoprotein cholesterol ; Cholesterol (97C5T2UQ7J)
Language	English
Publishing date	2022-03-14
Publishing country	Japan
Document type	Journal Article
ZDB-ID	2218264-0
ISSN	1347-3352 ; 1345-8957
ISSN (online)	1347-3352
ISSN	1345-8957
DOI	10.5650/jos.ess21348
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Article ; Online: Current status of intestinal transplantation in East Asia.

Hibi, Taizo / Chen, Yun / Kim, Ji-Il / Lee, Myung Duk / Matsuura, Toshiharu / Ueno, Takehisa

Current opinion in organ transplantation

2020 Volume 25, Issue 2, Page(s) 165–168

Abstract: Purpose of review: Intestinal transplantation (ITx) activity remains low in East Asia. We conducted a multinational, retrospective study on patients who underwent ITx in Korea, Taiwan, and Japan, to provide an overview and to foresee future developments ...

Abstract	Purpose of review: Intestinal transplantation (ITx) activity remains low in East Asia. We conducted a multinational, retrospective study on patients who underwent ITx in Korea, Taiwan, and Japan, to provide an overview and to foresee future developments and collaborations in this region. Recent findings: Total 71 ITx were performed in 67 patients. Living donor ITx was most commonly conducted in Japan (n = 13). Despite the low caseload, all three countries demonstrated acceptable patient survival rates of approximately 70% at 5 years. Over 70% of recipients with a functioning graft were free from total parenteral nutrition. Summary: There is an urgent need to establish a nationwide and multinational registry of ITx recipients and patients with intestinal failure in East Asia. An efficient referral system to specialized intestinal rehabilitation and ITx centers and a multidisciplinary team approach is also warranted to provide state-of-the-art treatment for patients desperately waiting for a chance to survive.
MeSH term(s)	Humans ; Asia ; Intestinal Diseases/therapy ; Intestines/transplantation ; Retrospective Studies
Language	English
Publishing date	2020-02-13
Publishing country	United States
Document type	Journal Article ; Multicenter Study
ZDB-ID	1390429-2
ISSN	1531-7013 ; 1087-2418
ISSN (online)	1531-7013
ISSN	1087-2418
DOI	10.1097/MOT.0000000000000750
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Zs.A 5609: Show issues

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Article: Syphilis-associated membranous nephropathy successfully treated with amoxicillin.

Hazue, Ryo / Ueno, Toshiharu / Nozaki, Hiroyuki / Kinowaki, Keiichi / Ohashi, Kenichi / Hoshino, Junichi / Sawa, Naoki / Ubara, Yoshifumi

Clinical nephrology

2021 Volume 96, Issue 5, Page(s) 297–301

Abstract: A 33-year-old Japanese man with no significant past medical history was admitted to our hospital for evaluation of weight gain and pitting edema. A laboratory test confirmed nephrotic-range proteinuria. Renal biopsy showed subepithelial deposits, and ... ...

Abstract	A 33-year-old Japanese man with no significant past medical history was admitted to our hospital for evaluation of weight gain and pitting edema. A laboratory test confirmed nephrotic-range proteinuria. Renal biopsy showed subepithelial deposits, and membranous nephropathy (MN) was diagnosed. Closer examination clarified an active syphilis infection. After renal biopsy, we prescribed amoxicillin for 8 weeks to treat the syphilis infection. Three weeks later, the patient's proteinuria dramatically decreased. This case is of interest because syphilis can become a cause of acute-onset MN in younger adults, and the incidence of syphilis is increasing in Japan. .
MeSH term(s)	Adult ; Amoxicillin/therapeutic use ; Glomerulonephritis, Membranous/diagnosis ; Glomerulonephritis, Membranous/drug therapy ; Glomerulonephritis, Membranous/etiology ; Humans ; Japan ; Male ; Proteinuria ; Syphilis/complications ; Syphilis/diagnosis ; Syphilis/drug therapy
Chemical Substances	Amoxicillin (804826J2HU)
Language	English
Publishing date	2021-08-27
Publishing country	Germany
Document type	Case Reports ; Journal Article
ZDB-ID	185101-9
ISSN	0301-0430
ISSN	0301-0430
DOI	10.5414/CN110586
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Zs.A 873: Show issues

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Article ; Online: Secular trends in the prevalence of dementia based on a community-based complete enumeration in Japan: the Nakayama Study.

Shimizu, Hideaki / Mori, Takaaki / Yoshida, Taku / Tachibana, Ayumi / Ozaki, Tomoki / Yoshino, Yuta / Ochi, Shinichiro / Sonobe, Naomi / Matsumoto, Teruhisa / Komori, Kenjiro / Iga, Jun-Ichi / Ninomiya, Toshiharu / Ueno, Shu-Ichi / Ikeda, Manabu

Psychogeriatrics : the official journal of the Japanese Psychogeriatric Society

2022 Volume 22, Issue 5, Page(s) 631–641

Abstract: Background: The number of dementia patients is increasing worldwide, especially in Japan, which has the world's highest ageing population. The increase in the number of older people with dementia is a medical and socioeconomic problem that needs to be ... ...

Abstract	Background: The number of dementia patients is increasing worldwide, especially in Japan, which has the world's highest ageing population. The increase in the number of older people with dementia is a medical and socioeconomic problem that needs to be prevented, but the actual situation is still not fully understood. Methods: Four cross-sectional studies on dementia were conducted in 1997, 2004, 2012, and 2016 for complete enumeration of all residents aged 65 years and older. We examined the secular trends in the prevalence of all-cause dementia, Alzheimer's disease (AD), vascular dementia (VaD), and other/unclassified dementia. Results: The age-standardised prevalence of all-cause dementia significantly increased (4.5% in 1997, 5.7% in 2004, 5.3% in 2012, 9.5% in 2016; P for trend <0.05). Similar trends were observed for AD (1.7%, 3.0%, 2.5% and 4.9%, respectively; P for trend <0.05) and other/unclassified dementia (0.8%, 1.0%, 1.0% and 2.2%, respectively; P for trend <0.05), whereas no significant change in VaD was seen (2.1%, 1.8%, 1.8%, 2.4%, respectively; P for trend = 0.77). The crude prevalence of all-cause dementia and AD increased from 1997 to 2016 among participants aged 75-79 years and ≥85 years (all P for trend <0.05). Similar trends were observed for other/unclassified dementia among participants aged ≥80 years (all P for trend <0.05), but not in VaD. Conclusions: The prevalence of dementia has increased beyond the ageing of the population, suggesting that factors in addition to ageing are involved in the increase in the number of older people with dementia. To control the increase in the number of older people with dementia, elucidation of secular trends in the incidence, mortality, and prognosis of dementia as well as the factors that promote and protect against dementia, and development of preventive strategies are necessary.
MeSH term(s)	Aged ; Alzheimer Disease/epidemiology ; Cross-Sectional Studies ; Dementia/epidemiology ; Dementia, Vascular/epidemiology ; Humans ; Japan/epidemiology ; Prevalence ; Risk Factors
Language	English
Publishing date	2022-06-26
Publishing country	England
Document type	Journal Article
ZDB-ID	2213105-X
ISSN	1479-8301 ; 1346-3500
ISSN (online)	1479-8301
ISSN	1346-3500
DOI	10.1111/psyg.12865
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