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Article: [Postgraduate surgical training and system for board-certified surgeons in gastroenterology].

Miyakawa, Shuichi

2009 Volume 110, Issue 3, Page(s) 123–127

Abstract: The board-certified surgeon in gastroenterology is one of the subspecialties based on the board-certified general surgeon. Certification is given to those who pass the qualifying and certifying examinations administered by the Japanese Society of ... ...

Abstract	The board-certified surgeon in gastroenterology is one of the subspecialties based on the board-certified general surgeon. Certification is given to those who pass the qualifying and certifying examinations administered by the Japanese Society of Gastroenterological Surgery, after achieving specified standards of surgical experience and demonstrating excellence in presentation, publication, and training provided by the curriculum. The level of acquired surgical skill for the initial certification is the ability to perform surgery for the treatment of gastroenterological diseases at the lower-middle degree of difficulty under laparotomy or thoracotomy. As of January 2009, there were 4,554 (about 21.6%) board-certified surgeons in gastroenterology among the 12,087 members of the Japanese Society of Gastroenterological Surgery. Creating a database of gastroenterological surgeries plays an important role in the evaluation of training facilities and gastroenterological specialists, information disclosure to the public, and analysis of the balance between the supply of and demand for surgeons. It is necessary to correct the gap between the tentative plan proposed by the Association of Social Insurance Committees of Societies for Surgery (Gaihoren) and payment for surgical treatment and to establish a system to evaluate specialist surgeries fairly to provide greater incentive for surgeons to become board certified.
MeSH term(s)	Certification ; Education, Medical, Graduate ; Gastroenterology/education ; General Surgery/education ; Japan ; Medicine ; Specialization
Language	Japanese
Publishing date	2009-05
Publishing country	Japan
Document type	English Abstract ; Journal Article
ZDB-ID	412544-7
ISSN	1880-1129 ; 0301-4894
ISSN (online)	1880-1129
ISSN	0301-4894
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Article ; Online: A case of multisystem inflammatory syndrome in children in a Japanese boy: with discussion of cytokine profile.

Fukuda, Sayaka / Kaneta, Mio / Miyake, Mayuko / Ohya, Takashi / Miyakawa, Kei / Iwamoto, Mari / Ito, Shuichi

Modern rheumatology case reports

2021 Volume 5, Issue 2, Page(s) 442–447

Abstract: The new disease concept of multisystem inflammatory syndrome in children (MIS-C), which is a systemic inflammatory syndrome with multiple organ involvement after SARS-CoV2 infection, was established in 2020. MIS-C is common in Hispanic and black children ...

Abstract	The new disease concept of multisystem inflammatory syndrome in children (MIS-C), which is a systemic inflammatory syndrome with multiple organ involvement after SARS-CoV2 infection, was established in 2020. MIS-C is common in Hispanic and black children in Europe and North America, with few reports in East Asians. A significant portion of patients with MIS-C develop Kawasaki disease (KD)-like symptoms. Therefore, differential diagnosis is challenging, especially in East Asia, where KD is most prevalent. No Japanese cases have been reported in the literatures so far. We report a case of MIS-C in Japan with KD-like symptoms. A 9-year-old Japanese boy, who was infected with SARS-CoV2 1 month previously along with his family, was admitted to our hospital owing to fever for 6 d and erythema mainly in the groyne and pubic area. He also had conjunctivitis, strawberry tongue and diarrhoea. His laboratory findings were as follows: WBC, 12,840/µL (lymphocytes, 4%); CRP, 22.6 mg/dL, pro-calcitonin, 1.8 ng/mL (normal, <0.50 ng/mL); NT pro-BNP, 7627 pg/mL (<125 pg/mL); and troponin T, 0.14 ng/mL (<0.01 ng/mL). His cardiac function was normal. We initially diagnosed him with KD. His fever rapidly resolved with intravenous immunoglobulin (IVIG) and there were no coronary artery lesions. Desquamation of the fingers was observed later. Finally, a history of SARS-COV2 infection, his age, atypical skin rash, elevation of markers of inflammation and heart failure and lymphopenia suggested the diagnosis of MIS-C rather than KD. Differentiation between KD and MIS-C is necessary even in Japan, especially in patients with atypical features of KD.
MeSH term(s)	COVID-19/complications ; COVID-19/diagnosis ; Child ; Cytokines/blood ; Diagnosis, Differential ; Humans ; Japan ; Male ; Mucocutaneous Lymph Node Syndrome/diagnosis ; Systemic Inflammatory Response Syndrome/diagnosis
Chemical Substances	Cytokines
Language	English
Publishing date	2021-07-21
Publishing country	England
Document type	Case Reports
ISSN	2472-5625
ISSN (online)	2472-5625
DOI	10.1080/24725625.2021.1920140
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Article ; Online: NIGT1 family proteins exhibit dual mode DNA recognition to regulate nutrient response-associated genes in Arabidopsis.

Ueda, Yoshiaki / Nosaki, Shohei / Sakuraba, Yasuhito / Miyakawa, Takuya / Kiba, Takatoshi / Tanokura, Masaru / Yanagisawa, Shuichi

PLoS genetics

2020 Volume 16, Issue 11, Page(s) e1009197

Abstract: Fine-tuning of nutrient uptake and response is indispensable for maintenance of nutrient homeostasis in plants, but the details of underlying mechanisms remain to be elucidated. NITRATE-INDUCIBLE GARP-TYPE TRANSCRIPTIONAL REPRESSOR 1 (NIGT1) family ... ...

Abstract	Fine-tuning of nutrient uptake and response is indispensable for maintenance of nutrient homeostasis in plants, but the details of underlying mechanisms remain to be elucidated. NITRATE-INDUCIBLE GARP-TYPE TRANSCRIPTIONAL REPRESSOR 1 (NIGT1) family proteins are plant-specific transcriptional repressors that function as an important hub in the nutrient signaling network associated with the acquisition and use of nitrogen and phosphorus. Here, by yeast two-hybrid assays, bimolecular fluorescence complementation assays, and biochemical analysis with recombinant proteins, we show that Arabidopsis NIGT1 family proteins form a dimer via the interaction mediated by a coiled-coil domain (CCD) in their N-terminal regions. Electrophoretic mobility shift assays defined that the NIGT1 dimer binds to two different motifs, 5'-GAATATTC-3' and 5'-GATTC-N38-GAATC-3', in target gene promoters. Unlike the dimer of wild-type NIGT1 family proteins, a mutant variant that could not dimerize due to amino acid substitutions within the CCD had lower specificity and affinity to DNA, thereby losing the ability to precisely regulate the expression of target genes. Thus, expressing the wild-type and mutant NIGT1 proteins in the nigt1 quadruple mutant differently modified NIGT1-regulated gene expression and responses towards nitrate and phosphate. These results suggest that the CCD-mediated dimerization confers dual mode DNA recognition to NIGT1 family proteins, which is necessary to make proper controls of their target genes and nutrient responses. Intriguingly, two 5'-GATTC-3' sequences are present in face-to-face orientation within the 5'-GATTC-N38-GAATC-3' sequence or its complementary one, while two 5'-ATTC-3' sequences are present in back-to-back orientation within the 5'-GAATATTC-3' or its complementary one. This finding suggests a unique mode of DNA binding by NIGT1 family proteins and may provide a hint as to why target sequences for some transcription factors cannot be clearly determined.
MeSH term(s)	Amino Acid Motifs ; Arabidopsis/physiology ; Arabidopsis Proteins/metabolism ; DNA/genetics ; DNA/metabolism ; Gene Expression Regulation, Plant ; Metabolic Networks and Pathways/genetics ; Nutrients/metabolism ; Plants, Genetically Modified ; Promoter Regions, Genetic/genetics ; Protein Multimerization/physiology ; Repressor Proteins/metabolism
Chemical Substances	Arabidopsis Proteins ; Repressor Proteins ; DNA (9007-49-2)
Language	English
Publishing date	2020-11-02
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2186725-2
ISSN	1553-7404 ; 1553-7390
ISSN (online)	1553-7404
ISSN	1553-7390
DOI	10.1371/journal.pgen.1009197
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Article ; Online: Contact urticaria syndrome caused by sulfamethoxazole sodium contained in eye drops.

Fujiwara, Yuriko / Inomata, Naoko / Ishida, Shuichi / Miyakawa, Mami / Nomura, Yuki / Mitani, Naoko / Aihara, Michiko

Contact dermatitis

2017 Volume 77, Issue 1, Page(s) 56–57

MeSH term(s)	Adult ; Female ; Humans ; Ophthalmic Solutions/adverse effects ; Ophthalmic Solutions/chemistry ; Skin Tests ; Syndrome ; Thiazoles/adverse effects ; Thiazoles/analysis ; Urticaria/chemically induced
Chemical Substances	Ophthalmic Solutions ; Thiazoles
Language	English
Publishing date	2017-06-14
Publishing country	England
Document type	Case Reports ; Journal Article
ZDB-ID	193121-0
ISSN	1600-0536 ; 0105-1873
ISSN (online)	1600-0536
ISSN	0105-1873
DOI	10.1111/cod.12745
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Article: Persistence of Robust Humoral Immune Response in Coronavirus Disease 2019 Convalescent Individuals Over 12 Months After Infection.

Open forum infectious diseases

2021 Volume 9, Issue 2, Page(s) ofab626

Abstract: Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection elicits varying degrees of protective immunity conferred by neutralizing antibodies (nAbs). In this study, we report the persistence of nAb responses over 12 months after ...

Abstract	Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection elicits varying degrees of protective immunity conferred by neutralizing antibodies (nAbs). In this study, we report the persistence of nAb responses over 12 months after infection despite their decreasing trend noticed from 6 months. Methods: The study included sera from 497 individuals who had been infected with SARS-CoV-2 between January and August 2020. Samples were collected at 6 and 12 months after onset. The titers of immunoglobulin (Ig)G to the viral nucleocapsid protein (NP) and receptor-binding domain (RBD) of the spike protein were measured by chemiluminescence enzyme immunoassay. The nAb titer was determined using lentivirus-based pseudovirus or authentic virus. Results: Antibody titers of NP-IgG, RBD-IgG, and nAbs were higher in severe and moderate cases than in mild cases at 12 months after onset. Although the nAb levels were likely to confer adequate protection against wild-type viral infection, the neutralization activity to recently circulating variants in some of the mild cases (~30%) was undermined, implying the susceptibility to reinfection with the variants of concerns (VOCs). Conclusions: Coronavirus disease 2019 convalescent individuals have robust humoral immunity even at 12 months after infection albeit that the medical history and background of patients could affect the function and dynamics of antibody response to the VOCs.
Language	English
Publishing date	2021-12-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	2757767-3
ISSN	2328-8957
ISSN	2328-8957
DOI	10.1093/ofid/ofab626
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Article ; Online: Loss of X-linked Protocadherin-19 differentially affects the behavior of heterozygous female and hemizygous male mice.

Hayashi, Shuichi / Inoue, Yoko / Hattori, Satoko / Kaneko, Mari / Shioi, Go / Miyakawa, Tsuyoshi / Takeichi, Masatoshi

Scientific reports

2017 Volume 7, Issue 1, Page(s) 5801

Abstract: Mutations in the X-linked gene Protocadherin-19 (Pcdh19) cause female-limited epilepsy and mental retardation in humans. Although Pcdh19 is known to be a homophilic cell-cell adhesion molecule, how its mutations bring about female-specific disorders ... ...

Abstract	Mutations in the X-linked gene Protocadherin-19 (Pcdh19) cause female-limited epilepsy and mental retardation in humans. Although Pcdh19 is known to be a homophilic cell-cell adhesion molecule, how its mutations bring about female-specific disorders remains elusive. Here, we report the effects of Pcdh19 knockout in mice on their development and behavior. Pcdh19 was expressed in various brain regions including the cerebral cortex and hippocampus. Although Pcdh19-positive cells were evenly distributed in layer V of wild-type cortices, their distribution became a mosaic in Pcdh19 heterozygous female cortices. In cortical and hippocampal neurons, Pcdh19 was localized along their dendrites, showing occasional accumulation on synapses. Pcdh19 mutants, however, displayed no detectable abnormalities in dendrite and spine morphology of layer V neurons. Nevertheless, Pcdh19 hemizygous males and heterozygous females showed impaired behaviors including activity defects under stress conditions. Notably, only heterozygous females exhibited decreased fear responses. In addition, Pcdh19 overexpression in wild-type cortices led to ectopic clustering of Pcdh19-positive neurons. These results suggest that Pcdh19 is required for behavioral control in mice, but its genetic loss differentially affects the male and female behavior, as seen in human, and they also support the hypothesis that the mosaic expression of Pcdh19 in brains perturbs neuronal interactions.
MeSH term(s)	Animals ; Behavior, Animal ; Cadherins/deficiency ; Cadherins/genetics ; Cerebral Cortex/pathology ; Female ; Hemizygote ; Heterozygote ; Hippocampus/pathology ; Male ; Mice ; Mice, Knockout
Chemical Substances	Cadherins ; Pcdh19 protein, mouse
Language	English
Publishing date	2017-07-19
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-017-06374-x
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Article ; Online: Updated clinical practice guidelines for the management of biliary tract cancers: revision concepts and major revised points.

Yoshitomi, Hideyuki / Miyakawa, Shuichi / Nagino, Masato / Takada, Tadahiro / Miyazaki, Masaru

Journal of hepato-biliary-pancreatic sciences

2015 Volume 22, Issue 4, Page(s) 274–278

Abstract: Background: In 2008, the Japanese Society of Hepato-Biliary-Pancreatic Surgery (JSHBPS) launched the clinical practice guideline for the management of biliary tract cancers. JSHBPS decided to revise these guidelines for distribution of updated points ... ...

Abstract	Background: In 2008, the Japanese Society of Hepato-Biliary-Pancreatic Surgery (JSHBPS) launched the clinical practice guideline for the management of biliary tract cancers. JSHBPS decided to revise these guidelines for distribution of updated points concerning the treatment of biliary tract cancers. Methods: To make clearer recommendations, we introduced the concepts of Grading of Recommendations Assessment, Development, and Evaluation (GRADE) approach, in which the strength of recommendations are decided considering not only quality of evidence, but also balance of benefits and harms/burdens, patients' preferences, and cost benefits. Results: We emphasize the importance of the dynamic contrast enhanced multiple row detector CT (MDCT) in the diagnosis of biliary tract and gallbladder carcinomas. For biliary drainage, we suggest to perform endoscopic approaches instead of percutaneous approach to avoid complications. Regarding the surgical treatments, we included new clinical questions about the importance of combined vascular resection, intraoperative histological examination of the bile duct resection margin, and the combined extrahepatic bile duct resection for the gallbladder carcinoma. We also discussed details about premalignant lesions and non-neoplastic lesions in pathology section. Conclusion: With this major revision, we expect that the Japanese standards of treatments of these diseases are recorded and reported in the universal language.
MeSH term(s)	Biliary Tract Neoplasms/surgery ; Biliary Tract Surgical Procedures/standards ; Disease Management ; Humans ; Practice Guidelines as Topic ; Reoperation
Language	English
Publishing date	2015-04
Publishing country	Japan
Document type	Journal Article ; Review
ZDB-ID	2536236-7
ISSN	1868-6982 ; 1868-6974
ISSN (online)	1868-6982
ISSN	1868-6974
DOI	10.1002/jhbp.234
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Article ; Online: Revision concepts and distinctive points of the new Japanese classification for biliary tract cancers in comparison with the 7(th) edition of the Union for International Cancer Control and the American Joint Committee on Cancer staging system.

Ohtsuka, Masayuki / Miyakawa, Shuichi / Nagino, Masato / Takada, Tadahiro / Miyazaki, Masaru

Journal of hepato-biliary-pancreatic sciences

2015 Volume 22, Issue 3, Page(s) 197–201

Abstract: Background: The 3(rd) English edition of the Japanese classification of the biliary tract cancers (JC) is now available in this journal. The primary aim of this revision is to provide all clinicians and researchers with a common language of cancer ... ...

Abstract	Background: The 3(rd) English edition of the Japanese classification of the biliary tract cancers (JC) is now available in this journal. The primary aim of this revision is to provide all clinicians and researchers with a common language of cancer staging at an international level. On the other hand, there are several important issues that should be solved for the optimization of the staging system. Methods: Revision concepts and major revision points of the 3(rd) English edition of the JC were reviewed. Furthermore, comparing with the 7(th) edition of staging system developed by the Union for International Cancer Control (UICC) and the American Joint Committee on Cancer (AJCC), distinctive points in the JC was discussed. Results: In this edition of the JC, the same stage groupings as those in the UICC/AJCC staging system were basically adopted. T, N, and M categories were also identical in principle with those in the UICC/AJCC staging system, although slight modifications were proposed as the "Japanese rules". As distinctive points, perihilar cholangiocarcinomas and ampullary region carcinomas were clearly defined. Intraepithelial tumor was discriminated from invasive carcinoma at ductal resection margins. Classifications of site-specific surgical margin status remained in this edition. Histological classification was based on that in the former editions of the JC, but adopted some parts of the World Health Organization classification. Conclusions: The JC now share its staging system of the biliary tact carcinomas with the UICC/AJCC staging system. Future validation of the "Japanese rules" could provide important evidence to make globally standardized staging system.
MeSH term(s)	Bile Duct Neoplasms/classification ; Bile Duct Neoplasms/diagnosis ; Humans ; International Cooperation ; Japan ; Neoplasm Staging ; United States
Language	English
Publishing date	2015-03
Publishing country	Japan
Document type	Journal Article ; Review
ZDB-ID	2536236-7
ISSN	1868-6982 ; 1868-6974
ISSN (online)	1868-6982
ISSN	1868-6974
DOI	10.1002/jhbp.212
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Article ; Online: Loss of X-linked Protocadherin-19 differentially affects the behavior of heterozygous female and hemizygous male mice

Shuichi Hayashi / Yoko Inoue / Satoko Hattori / Mari Kaneko / Go Shioi / Tsuyoshi Miyakawa / Masatoshi Takeichi

Scientific Reports, Vol 7, Iss 1, Pp 1-

2017 Volume 15

Abstract: Abstract Mutations in the X-linked gene Protocadherin-19 (Pcdh19) cause female-limited epilepsy and mental retardation in humans. Although Pcdh19 is known to be a homophilic cell-cell adhesion molecule, how its mutations bring about female-specific ... ...

Abstract	Abstract Mutations in the X-linked gene Protocadherin-19 (Pcdh19) cause female-limited epilepsy and mental retardation in humans. Although Pcdh19 is known to be a homophilic cell-cell adhesion molecule, how its mutations bring about female-specific disorders remains elusive. Here, we report the effects of Pcdh19 knockout in mice on their development and behavior. Pcdh19 was expressed in various brain regions including the cerebral cortex and hippocampus. Although Pcdh19-positive cells were evenly distributed in layer V of wild-type cortices, their distribution became a mosaic in Pcdh19 heterozygous female cortices. In cortical and hippocampal neurons, Pcdh19 was localized along their dendrites, showing occasional accumulation on synapses. Pcdh19 mutants, however, displayed no detectable abnormalities in dendrite and spine morphology of layer V neurons. Nevertheless, Pcdh19 hemizygous males and heterozygous females showed impaired behaviors including activity defects under stress conditions. Notably, only heterozygous females exhibited decreased fear responses. In addition, Pcdh19 overexpression in wild-type cortices led to ectopic clustering of Pcdh19-positive neurons. These results suggest that Pcdh19 is required for behavioral control in mice, but its genetic loss differentially affects the male and female behavior, as seen in human, and they also support the hypothesis that the mosaic expression of Pcdh19 in brains perturbs neuronal interactions.
Keywords	Medicine ; R ; Science ; Q
Subject code	590
Language	English
Publishing date	2017-07-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Biliary tract cancer registry in Japan from 2008 to 2013.

Ishihara, Shin / Horiguchi, Akihiko / Miyakawa, Shuichi / Endo, Itaru / Miyazaki, Masaru / Takada, Tadahiro

Journal of hepato-biliary-pancreatic sciences

2016 Volume 23, Issue 3, Page(s) 149–157

Abstract: Background: The present study analyzed biliary tract cancer patients registered from 2008 to 2013 in Japan and evaluated the outcomes of biliary tract cancer.: Methods: A total of 18,606 patients were registered from 2008 to 2013. Cases were analyzed ...

Abstract	Background: The present study analyzed biliary tract cancer patients registered from 2008 to 2013 in Japan and evaluated the outcomes of biliary tract cancer. Methods: A total of 18,606 patients were registered from 2008 to 2013. Cases were analyzed with regard to patient survival according to contiguous extent of the primary tumor (T), node metastasis, and tumor stage using the 3rd English edition of the Japanese classification of the biliary tract cancers. Results: Five-year survival rates were 39.8% for gallbladder cancer, 24.2% for perihilar bile duct cancer, 39.1% for distal bile duct cancer, and 61.3% for ampullary region cancer. Significant differences were observed between newly introduced subdivisions in the new Japanese classification for all tumoral sites except gallbladder cancer. The survival rate in patients with #13a metastasis was significantly higher than in patients with distant lymph node metastasis. Conclusions: The new Japanese classification adopted the 7th edition of staging system developed by the Union for International Cancer Control staging system. However, numerous aspects of these classification systems remain unvalidated. The present analysis demonstrated the significance of a proportion of T factor subdivisions and classifications of regional lymph nodes in cases of gallbladder cancer in the new Japanese classification.
MeSH term(s)	Biliary Tract Neoplasms/epidemiology ; Biliary Tract Neoplasms/pathology ; Female ; Humans ; Japan/epidemiology ; Lymphatic Metastasis ; Male ; Neoplasm Staging ; Registries ; Survival Rate
Language	English
Publishing date	2016-03
Publishing country	Japan
Document type	Journal Article
ZDB-ID	2536236-7
ISSN	1868-6982 ; 1868-6974
ISSN (online)	1868-6982
ISSN	1868-6974
DOI	10.1002/jhbp.314
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