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  1. AU=Uehara Akira
  2. AU=Fransen Justin H AU=Fransen Justin H
  3. AU="Memon, Roha Saeed"
  4. AU="Lipworth, Samuel"
  5. AU="Killian, Michael O"
  6. AU=Smaldino Paul E.
  7. AU=Bi Hai
  8. AU="Pintore, Giorgio"
  9. AU="Signorini C."
  10. AU="Mameli, Maria Sabrina"
  11. AU="Yong-ming GAO"
  12. AU="Paquette, Kimberly"
  13. AU="Sharawat, Indar Kumar"
  14. AU="Alexandre Alanio"
  15. AU="Caron, Jeffrey G"
  16. AU="Lubisi, Baratang A"
  17. AU="Edelman, Robert R."
  18. AU="van der Werf, Steffie"
  19. AU="Sam, Andrew"

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  1. Artikel ; Online: Immunohistochemical study of dissociation and association of adherens junctions in splenic sinus endothelial cells.

    Uehara, Kiyoko / Uehara, Akira

    Cell and tissue research

    2021  Band 384, Heft 1, Seite(n) 25–33

    Abstract: It is not yet clear whether cellular junctions between splenic sinus endothelial cells are open or closed. In order to clarify this, immunolocalization of thrombomodulin (TM), endothelial protein C receptor (EPCR), protease-activated receptor 1 (PAR1), ... ...

    Abstract It is not yet clear whether cellular junctions between splenic sinus endothelial cells are open or closed. In order to clarify this, immunolocalization of thrombomodulin (TM), endothelial protein C receptor (EPCR), protease-activated receptor 1 (PAR1), sphingosine 1-phosphate receptor 1 (S1P
    Mesh-Begriff(e) Adherens Junctions/metabolism ; Animals ; Endothelial Cells/metabolism ; Humans ; Immunohistochemistry/methods ; Male ; Rats ; Rats, Wistar ; Spleen/cytology
    Sprache Englisch
    Erscheinungsdatum 2021-03-03
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 125067-x
    ISSN 1432-0878 ; 0302-766X
    ISSN (online) 1432-0878
    ISSN 0302-766X
    DOI 10.1007/s00441-021-03426-7
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  2. Artikel ; Online: Immunolocalization of protease-activated receptors in endothelial cells of splenic sinuses.

    Uehara, Kiyoko / Uehara, Akira

    Cell and tissue research

    2021  Band 386, Heft 3, Seite(n) 605–615

    Abstract: The immunolocalization of protease-activated receptors (PARs) and related proteins in splenic sinus endothelial cells was examined using immunofluorescence and electron microscopy. Immunofluorescence microscopy showed that PAR1 colocalized with PAR2, ... ...

    Abstract The immunolocalization of protease-activated receptors (PARs) and related proteins in splenic sinus endothelial cells was examined using immunofluorescence and electron microscopy. Immunofluorescence microscopy showed that PAR1 colocalized with PAR2, PAR3, and PAR4. PAR4 colocalized with PAR3 and P2Y12. Myosin heavy chain IIA localized to the outer shape and at the base of cells, but did not colocalize with α-catenin. The localization of di-phosphorylated myosin regulatory light chains (ppMLC) was partially detected on the outer circumference and conspicuously at the base of cells. Macrophage migration inhibitory factor (MIF) also localized in cells. Immunogold electron microscopy revealed the localization of PAR1 on the caveolar membrane, plasma membrane, and junctional membrane of cells. PAR2 and PAR3 localized to the plasma membrane of cells. PAR4 localized to the plasma membrane, depressions in the plasma membrane, and cytoplasmic vesicles. PpMLC was detected in stress fibers, but rarely near the adherens junctions of neighboring cells. MIF localized in vesicles on the apical and basal sides of the Golgi apparatus. Electron microscopy of endothelial cells with saponin extraction showed the depression of many coated pits formed by clathrin from the plasma membrane. Stress fibers developed at the base of cells; however, few actin filaments were observed near adherens junctions. These results indicate that PARs play important roles in splenic sinus endothelial cells, such as in endothelial barrier protection and the maintenance of firm adhesion to ring fibers.
    Mesh-Begriff(e) Animals ; Endothelial Cells/metabolism ; Humans ; Peptide Hydrolases/metabolism ; Rats ; Spleen/ultrastructure
    Chemische Substanzen Peptide Hydrolases (EC 3.4.-)
    Sprache Englisch
    Erscheinungsdatum 2021-10-06
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 125067-x
    ISSN 1432-0878 ; 0302-766X
    ISSN (online) 1432-0878
    ISSN 0302-766X
    DOI 10.1007/s00441-021-03535-3
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Peptide coupling using recyclable bicyclic benziodazolone.

    Uehara, Daigo / Adachi, Sota / Tsubouchi, Akira / Okada, Yohei / Zhdankin, Viktor V / Yoshimura, Akira / Saito, Akio

    Chemical communications (Cambridge, England)

    2024  Band 60, Heft 8, Seite(n) 956–959

    Abstract: We report a greener peptide coupling using bicyclic benziodazolone and triarylphosphine as coupling reagents. Bicyclic benziodazolone also works as a base and can be recovered as the corresponding iodine(I) compound after use, which can be converted to ... ...

    Abstract We report a greener peptide coupling using bicyclic benziodazolone and triarylphosphine as coupling reagents. Bicyclic benziodazolone also works as a base and can be recovered as the corresponding iodine(I) compound after use, which can be converted to the original iodine(III) reagent by electrolytic oxidation.
    Sprache Englisch
    Erscheinungsdatum 2024-01-23
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 1472881-3
    ISSN 1364-548X ; 1359-7345 ; 0009-241X
    ISSN (online) 1364-548X
    ISSN 1359-7345 ; 0009-241X
    DOI 10.1039/d3cc04431a
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel: Immunolocalization of protease-activated receptors in endothelial cells of splenic sinuses

    Uehara, Kiyoko / Uehara, Akira

    Cell and tissue research. 2021 Dec., v. 386, no. 3

    2021  

    Abstract: The immunolocalization of protease-activated receptors (PARs) and related proteins in splenic sinus endothelial cells was examined using immunofluorescence and electron microscopy. Immunofluorescence microscopy showed that PAR1 colocalized with PAR2, ... ...

    Abstract The immunolocalization of protease-activated receptors (PARs) and related proteins in splenic sinus endothelial cells was examined using immunofluorescence and electron microscopy. Immunofluorescence microscopy showed that PAR1 colocalized with PAR2, PAR3, and PAR4. PAR4 colocalized with PAR3 and P2Y12. Myosin heavy chain IIA localized to the outer shape and at the base of cells, but did not colocalize with α-catenin. The localization of di-phosphorylated myosin regulatory light chains (ppMLC) was partially detected on the outer circumference and conspicuously at the base of cells. Macrophage migration inhibitory factor (MIF) also localized in cells. Immunogold electron microscopy revealed the localization of PAR1 on the caveolar membrane, plasma membrane, and junctional membrane of cells. PAR2 and PAR3 localized to the plasma membrane of cells. PAR4 localized to the plasma membrane, depressions in the plasma membrane, and cytoplasmic vesicles. PpMLC was detected in stress fibers, but rarely near the adherens junctions of neighboring cells. MIF localized in vesicles on the apical and basal sides of the Golgi apparatus. Electron microscopy of endothelial cells with saponin extraction showed the depression of many coated pits formed by clathrin from the plasma membrane. Stress fibers developed at the base of cells; however, few actin filaments were observed near adherens junctions. These results indicate that PARs play important roles in splenic sinus endothelial cells, such as in endothelial barrier protection and the maintenance of firm adhesion to ring fibers.
    Schlagwörter Golgi apparatus ; actin ; adhesion ; clathrin ; electron microscopy ; fluorescence microscopy ; fluorescent antibody technique ; macrophage migration inhibitory factors ; myosin heavy chains ; plasma membrane ; research ; saponins
    Sprache Englisch
    Erscheinungsverlauf 2021-12
    Umfang p. 605-615.
    Erscheinungsort Springer Berlin Heidelberg
    Dokumenttyp Artikel
    ZDB-ID 125067-x
    ISSN 1432-0878 ; 0302-766X
    ISSN (online) 1432-0878
    ISSN 0302-766X
    DOI 10.1007/s00441-021-03535-3
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  5. Artikel: Immunohistochemical study of dissociation and association of adherens junctions in splenic sinus endothelial cells

    Uehara, Kiyoko / Uehara, Akira

    Cell and tissue research. 2021 Apr., v. 384, no. 1

    2021  

    Abstract: It is not yet clear whether cellular junctions between splenic sinus endothelial cells are open or closed. In order to clarify this, immunolocalization of thrombomodulin (TM), endothelial protein C receptor (EPCR), protease-activated receptor 1 (PAR1), ... ...

    Abstract It is not yet clear whether cellular junctions between splenic sinus endothelial cells are open or closed. In order to clarify this, immunolocalization of thrombomodulin (TM), endothelial protein C receptor (EPCR), protease-activated receptor 1 (PAR1), sphingosine 1-phosphate receptor 1 (S1P₁), β-catenin phosphorylated at Try142 (β-catenin Y142) and β-catenin phosphorylated at Try654 (β-catenin Y654), which are related proteins that regulate dissociation and association of the adherens junctions of endothelial cells, are examined in rats using laser microscopy and electron microscopy. TM, EPCR, PAR1 and S1P₁ were colocalized in the entire circumference of the endothelial cells, as well as in the caveolar membranes and junctional membranes of adjacent endothelial cells. These molecules may protect the adherens junctions of the endothelial cells. On the other hand, β-catenin Y142 and β-catenin Y 654 colocalized with α-catenin and β-catenin, respectively and in addition, β-catenin Y142 and β-catenin Y 654 were localized in the vicinity of the adherens junctions of the endothelial cells from immunogold electron microcopy. The adherens junctions are considered to be partially dissociated at the site where β-catenin Y142 and β-catenin Y 654 are localized. Thus, the system that protects the adherens junctions and the system that dissociates them may concurrently coexist in the endothelial cells and dissociation and association of the adherens junctions may be constantly repeated at the cell boundary of the endothelial cells.
    Schlagwörter dissociation ; electron microscopy ; immunohistochemistry ; research ; sphingosine
    Sprache Englisch
    Erscheinungsverlauf 2021-04
    Umfang p. 25-33.
    Erscheinungsort Springer Berlin Heidelberg
    Dokumenttyp Artikel
    Anmerkung NAL-AP-2-clean
    ZDB-ID 125067-x
    ISSN 1432-0878 ; 0302-766X
    ISSN (online) 1432-0878
    ISSN 0302-766X
    DOI 10.1007/s00441-021-03426-7
    Datenquelle NAL Katalog (AGRICOLA)

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  6. Artikel ; Online: A Fatal Case of Disseminated Infection Caused by Community-Associated Methicillin-Resistant Staphylococcus aureus USA300 Clone.

    Ote, Hiroyuki / Ito, Hideyuki / Akira, Taroh / Sugai, Motoyuki / Hisatsune, Junzo / Uehara, Yuki / Oba, Yuichiro

    Japanese journal of infectious diseases

    2023  Band 76, Heft 4, Seite(n) 251–254

    Abstract: Methicillin-resistant Staphylococcus aureus (MRSA) USA300 is a representative community-associated MRSA (CA-MRSA) clone worldwide. Herein, we report the case of a patient with USA300 clone infection who could not be salvaged. A 25-year-old man who had ... ...

    Abstract Methicillin-resistant Staphylococcus aureus (MRSA) USA300 is a representative community-associated MRSA (CA-MRSA) clone worldwide. Herein, we report the case of a patient with USA300 clone infection who could not be salvaged. A 25-year-old man who had sex with men presented with symptoms including fever persisting for one week and skin lesions located on the buttocks. Computed tomography imaging showed multiple nodules and consolidations, especially in the peripheral lung fields, right iliac vein thrombosis, and pyogenic myositis of medial thighs bilaterally. Blood cultures revealed MRSA bacteremia. The patient's condition deteriorated rapidly, complicated by acute respiratory distress syndrome and infective endocarditis. Despite the intubation on the 6th hospital day, he died on the 9th day. Multilocus sequence typing of this patient's MRSA strain revealed sequence type 8 with a staphylococcal cassette chromosome of mec type IVa, Panton-Valentine leukocidin gene, and the arginine catabolic mobile element, indicating presence of the USA300 clone. Patients with CA-MRSA skin lesions presenting with furuncles or carbuncles on the lower body are at a higher risk of severe disease. The patient's background, appearance, and location of skin lesions are critical for the early diagnosis of severe CA-MRSA infection.
    Mesh-Begriff(e) Male ; Humans ; Adult ; Methicillin-Resistant Staphylococcus aureus/genetics ; Staphylococcal Infections/diagnosis ; Multilocus Sequence Typing ; Bacteremia ; Community-Acquired Infections/diagnosis
    Sprache Englisch
    Erscheinungsdatum 2023-03-31
    Erscheinungsland Japan
    Dokumenttyp Case Reports ; Journal Article
    ZDB-ID 1478383-6
    ISSN 1884-2836 ; 1344-6304
    ISSN (online) 1884-2836
    ISSN 1344-6304
    DOI 10.7883/yoken.JJID.2022.578
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  7. Artikel ; Online: Drug-induced Steatohepatitis Caused by Long-term Use of Topical Steroids for Atopic Dermatitis: A Case Report.

    Tamura, Yuki / Naganuma, Atsushi / Suzuki, Yuhei / Uehara, Sanae / Hosino, Takashi / Hatanaka, Takeshi / Shibusawa, Nobuyuki / Uehara, Akihito / Ogawa, Akira / Kakizaki, Satoru / Uraoka, Toshio

    Internal medicine (Tokyo, Japan)

    2024  

    Abstract: Atopic dermatitis is common in children and often treated with topical corticosteroids (TCs). A boy in his late teens who had been using TCs for atopic dermatitis was diagnosed with liver damage during a health checkup. A medical examination revealed ... ...

    Abstract Atopic dermatitis is common in children and often treated with topical corticosteroids (TCs). A boy in his late teens who had been using TCs for atopic dermatitis was diagnosed with liver damage during a health checkup. A medical examination revealed severe steatotic liver disease and elevated liver enzyme levels despite the absence of typical symptoms such as central obesity. After discontinuation of TCs, an improvement in liver enzyme levels was observed, leading to the diagnosis of drug-induced steatohepatitis. This case underscores the potential liver risks associated with prolonged TC use in children, highlighting the need for parental education.
    Sprache Englisch
    Erscheinungsdatum 2024-04-09
    Erscheinungsland Japan
    Dokumenttyp Journal Article
    ZDB-ID 32371-8
    ISSN 1349-7235 ; 0021-5120 ; 0918-2918
    ISSN (online) 1349-7235
    ISSN 0021-5120 ; 0918-2918
    DOI 10.2169/internalmedicine.3439-23
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Artikel: [A Case of Transverse Colon Cancer with Gastrointestinal Amyloidosis Prevented from Anastomotic Leakage by Multidimensional Approach].

    Kusudo, Natsuki / Suzuki, Takuya / Uehara, Shuhei / Kato, Akira / Fujii, Yoshiaki / Harata, Shinnosuke / Watanabe, Kaori / Yanagita, Takeshi / Ushigome, Hajime / Ogawa, Ryo / Takahashi, Hiroki / Matsuo, Yoichi / Mitsui, Akira / Kimura, Masahiro / Takiguchi, Shuji

    Gan to kagaku ryoho. Cancer & chemotherapy

    2024  Band 51, Heft 1, Seite(n) 90–92

    Abstract: The patient was a 68-year-old woman who was on hemodialysis due to systemic amyloidosis and nephrotic syndrome. Biopsy revealed amyloid deposition in the stomach, duodenum, and colon. A transverse colon tumor was found on a follow- up CT after the aortic ...

    Abstract The patient was a 68-year-old woman who was on hemodialysis due to systemic amyloidosis and nephrotic syndrome. Biopsy revealed amyloid deposition in the stomach, duodenum, and colon. A transverse colon tumor was found on a follow- up CT after the aortic dissection surgery. We performed lower gastrointestinal endoscopy and contrast-enhanced CT and diagnosed transverse colon cancer with gastric wall infiltration(cStage Ⅲc). We considered that transverse colon resection was oncologically sufficient. However, due to concurrent gastrointestinal amyloidosis, which increased the risk of anastomotic leakage we performed laparoscopic extended right hemicolectomy to avoid colon-colon anastomosis with partial gastrectomy. Additionally intraoperative indocyanine green(ICG)fluorescence imaging showed that the fluorescence signal in the small intestinal wall was satisfactory, while it was weak in the colon wall. As a result, we suspected of impaired blood flow of colon wall due to an amyloidosis, so we additionally created a loop ileostomy. It is said that gastrointestinal amyloidosis raises the risk of anastomotic leakage. A case of transverse colon cancer complicated by gastrointestinal amyloidosis in which we successfully prevented anastomotic leakage through a multidimensional evaluation and approach is reported, along with a literature review.
    Mesh-Begriff(e) Female ; Humans ; Aged ; Anastomotic Leak ; Colon, Transverse/surgery ; Amyloidosis/complications ; Amyloidosis/surgery ; Colonic Neoplasms/complications ; Colonic Neoplasms/surgery ; Gastrointestinal Diseases
    Sprache Japanisch
    Erscheinungsdatum 2024-01-22
    Erscheinungsland Japan
    Dokumenttyp Review ; Case Reports ; English Abstract ; Journal Article
    ZDB-ID 604842-0
    ISSN 0385-0684
    ISSN 0385-0684
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  9. Artikel ; Online: Author Correction: Gait improvement with wearable cyborg HAL trunk unit for parkinsonian patients: five case reports.

    Uehara, Akira / Kawamoto, Hiroaki / Imai, Hisamasa / Shirai, Makoto / Sone, Masatomi / Noda, Sachiko / Sato, Shigeto / Hattori, Nobutaka / Sankai, Yoshiyuki

    Scientific reports

    2023  Band 13, Heft 1, Seite(n) 8247

    Sprache Englisch
    Erscheinungsdatum 2023-05-22
    Erscheinungsland England
    Dokumenttyp Published Erratum
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-35421-z
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  10. Artikel ; Online: Low-grade mixed neuroendocrine-non-neuroendocrine neoplasm of the extrahepatic bile duct: A rare tumour with 11 years' follow-up before surgery.

    Kubota, Koji / Sato, Yoshinori / Iwaya, Mai / Notake, Tsuyoshi / Shimizu, Akira / Iguchi, Takuya / Uehara, Takeshi / Ota, Hiroyoshi / Soejima, Yuji

    Pathology international

    2023  Band 73, Heft 4, Seite(n) 173–176

    Mesh-Begriff(e) Humans ; Follow-Up Studies ; Neuroendocrine Tumors/surgery ; Neuroendocrine Tumors/pathology ; Bile Ducts, Extrahepatic/surgery ; Bile Ducts, Extrahepatic/pathology ; Bile Duct Neoplasms/surgery ; Bile Duct Neoplasms/pathology
    Sprache Englisch
    Erscheinungsdatum 2023-03-07
    Erscheinungsland Australia
    Dokumenttyp Letter
    ZDB-ID 1194850-4
    ISSN 1440-1827 ; 1320-5463
    ISSN (online) 1440-1827
    ISSN 1320-5463
    DOI 10.1111/pin.13317
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