Article ; Online: G1-Cyclin2 (Cln2) promotes chromosome hypercondensation in eco1/ctf7 rad61 null cells during hyperthermic stress in Saccharomyces cerevisiae.
2022 Volume 12, Issue 8
Abstract: ... result in severe developmental abnormalities and intellectual disabilities referred to as Roberts ...
| Abstract | Eco1/Ctf7 is a highly conserved acetyltransferase that activates cohesin complexes and is critical for sister chromatid cohesion, chromosome condensation, DNA damage repair, nucleolar integrity, and gene transcription. Mutations in the human homolog of ECO1 (ESCO2/EFO2), or in genes that encode cohesin subunits, result in severe developmental abnormalities and intellectual disabilities referred to as Roberts syndrome and Cornelia de Lange syndrome, respectively. In yeast, deletion of ECO1 results in cell inviability. Codeletion of RAD61 (WAPL in humans), however, produces viable yeast cells. These eco1 rad61 double mutants, however, exhibit a severe temperature-sensitive growth defect, suggesting that Eco1 or cohesins respond to hyperthermic stress through a mechanism that occurs independent of Rad61. Here, we report that deletion of the G1 cyclin CLN2 rescues the temperature-sensitive lethality otherwise exhibited by eco1 rad61 mutant cells, such that the triple mutant cells exhibit robust growth over a broad range of temperatures. While Cln1, Cln2, and Cln3 are functionally redundant G1 cyclins, neither CLN1 nor CLN3 deletions rescue the temperature-sensitive growth defects otherwise exhibited by eco1 rad61 double mutants. We further provide evidence that CLN2 deletion rescues hyperthermic growth defects independent of START and impacts the state of chromosome condensation. These findings reveal novel roles for Cln2 that are unique among the G1 cyclin family and appear critical for cohesin regulation during hyperthermic stress. |
|---|---|
| MeSH term(s) | Acetyltransferases/genetics ; Cell Cycle Proteins/genetics ; Chromatids ; Chromosomal Proteins, Non-Histone/genetics ; Chromosome Segregation ; Cyclins/genetics ; Humans ; Lymphocytes, Null ; Membrane Glycoproteins/genetics ; Molecular Chaperones/genetics ; Nuclear Proteins/genetics ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae Proteins/genetics |
| Chemical Substances | CLN2 protein, S cerevisiae ; CLN3 protein, human ; Cell Cycle Proteins ; Chromosomal Proteins, Non-Histone ; Cyclins ; Membrane Glycoproteins ; Molecular Chaperones ; Nuclear Proteins ; RAD61 protein, S cerevisiae ; Saccharomyces cerevisiae Proteins ; Acetyltransferases (EC 2.3.1.-) ; ECO1 protein, S cerevisiae (EC 2.3.1.-) ; ESCO2 protein, human (EC 2.3.1.-) |
| Language | English |
| Publishing date | 2022-06-22 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, N.I.H., Extramural |
| ZDB-ID | 2629978-1 |
| ISSN | 2160-1836 ; 2160-1836 |
| ISSN (online) | 2160-1836 |
| ISSN | 2160-1836 |
| DOI | 10.1093/g3journal/jkac157 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.