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  1. Article ; Online: Temporal resolution of NAIL-MS of tRNA, rRNA and Poly-A RNA is overcome by actinomycin D.

    Hagelskamp, Felix / Borland, Kayla / Ammann, Gregor / Kaiser, Stefanie M

    RSC chemical biology

    2023  Volume 4, Issue 5, Page(s) 354–362

    Abstract: RNA is dynamically modified and has the potential to respond to environmental changes and tune translation. The objective of this work is to uncover the temporal limitation of our recently developed cell culture NAIL-MS (nucleic acid isotope labelling ... ...

    Abstract RNA is dynamically modified and has the potential to respond to environmental changes and tune translation. The objective of this work is to uncover the temporal limitation of our recently developed cell culture NAIL-MS (nucleic acid isotope labelling coupled mass spectrometry) technology and overcome it. Actinomycin D (AcmD), an inhibitor of transcription, was used in the NAIL-MS context to reveal the origin of hybrid nucleoside signals composed of unlabelled nucleosides and labelled methylation marks. We find that the formation of these hybrid species depends exclusively on transcription for Poly-A RNA and rRNA but is partly transcription-independent for tRNA. This finding suggests that tRNA modifications adapt and are dynamically regulated by cells to overcome
    Language English
    Publishing date 2023-02-24
    Publishing country England
    Document type Journal Article
    ISSN 2633-0679
    ISSN (online) 2633-0679
    DOI 10.1039/d2cb00243d
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Polr3b heterozygosity in mice induces both beneficial and deleterious effects on health during ageing with no effect on lifespan.

    Borland, Gillian / Wilkie, Stephen E / Thomson, Jackie / Wang, Zhe / Tullet, Jennifer M A / Alic, Nazif / Selman, Colin

    Aging cell

    2024  , Page(s) e14141

    Abstract: The genetic pathways that modulate ageing in multicellular organisms are typically highly conserved across wide evolutionary distances. Recently RNA polymerase III (Pol III) was shown to promote ageing in yeast, C. elegans and D. melanogaster. In this ... ...

    Abstract The genetic pathways that modulate ageing in multicellular organisms are typically highly conserved across wide evolutionary distances. Recently RNA polymerase III (Pol III) was shown to promote ageing in yeast, C. elegans and D. melanogaster. In this study we investigated the role of Pol III in mammalian ageing using C57BL/6N mice heterozygous for Pol III (Polr3b
    Language English
    Publishing date 2024-03-11
    Publishing country England
    Document type Journal Article
    ZDB-ID 2113083-8
    ISSN 1474-9726 ; 1474-9718
    ISSN (online) 1474-9726
    ISSN 1474-9718
    DOI 10.1111/acel.14141
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    In stock of ZB MED Cologne/Königswinter

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  3. Article: Microbiome imaging goes à la carte: Incorporating click chemistry into the fluorescence-activating and absorption-shifting tag (FAST) imaging platform.

    Anderson, David M / Logan, Matthew G / Patty, Sara S / Kendall, Alexander J / Borland, Christina Z / Pfeifer, Carmem S / Kreth, Jens / Merritt, Justin L

    bioRxiv : the preprint server for biology

    2023  

    Abstract: The human microbiome is predominantly composed of facultative and obligate anaerobic bacteria that live in hypoxic/anoxic polymicrobial biofilm communities. Given the oxidative sensitivity of large fractions of the human microbiota, green fluorescent ... ...

    Abstract The human microbiome is predominantly composed of facultative and obligate anaerobic bacteria that live in hypoxic/anoxic polymicrobial biofilm communities. Given the oxidative sensitivity of large fractions of the human microbiota, green fluorescent protein (GFP) and related genetically-encoded fluorophores only offer limited utility for live cell imaging due the oxygen requirement for chromophore maturation. Consequently, new fluorescent imaging modalities are needed to study polymicrobial interactions and microbiome-host interactions within anaerobic environments. The fluorescence-activating and absorption shifting tag (FAST) is a rapidly developing genetically-encoded fluorescent imaging technology that exhibits tremendous potential to address this need. In the FAST system, fluorescence only occurs when the FAST protein is complexed with one of a suite of cognate small molecule fluorogens. To expand the utility of FAST imaging, we sought to develop a modular platform (Click-FAST) to democratize fluorogen engineering for personalized use cases. Using Click-FAST, investigators can quickly and affordably sample a vast chemical space of compounds, potentially imparting a broad range of desired functionalities to the parental fluorogen. In this work, we demonstrate the utility of the Click-FAST platform using a novel fluorogen,
    Language English
    Publishing date 2023-10-02
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.10.02.560575
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  4. Article ; Online: Aggression Results in the Phosphorylation of ERK1/2 in the Nucleus Accumbens and the Dephosphorylation of mTOR in the Medial Prefrontal Cortex in Female Syrian Hamsters.

    Borland, Johnathan M / Dempsey, Desarae A / Peyla, Anna C / Hall, Megan A L / Kohut-Jackson, Abigail L / Mermelstein, Paul G / Meisel, Robert L

    International journal of molecular sciences

    2023  Volume 24, Issue 2

    Abstract: Like many social behaviors, aggression can be rewarding, leading to behavioral plasticity. One outcome of reward-induced aggression is the long-term increase in the speed in which future aggression-based encounters is initiated. This form of aggression ... ...

    Abstract Like many social behaviors, aggression can be rewarding, leading to behavioral plasticity. One outcome of reward-induced aggression is the long-term increase in the speed in which future aggression-based encounters is initiated. This form of aggression impacts dendritic structure and excitatory synaptic neurotransmission in the nucleus accumbens, a brain region well known to regulate motivated behaviors. Yet, little is known about the intracellular signaling mechanisms that drive these structural/functional changes and long-term changes in aggressive behavior. This study set out to further elucidate the intracellular signaling mechanisms regulating the plasticity in neurophysiology and behavior that underlie the rewarding consequences of aggressive interactions. Female Syrian hamsters experienced zero, two or five aggressive interactions and the phosphorylation of proteins in reward-associated regions was analyzed. We report that aggressive interactions result in a transient increase in the phosphorylation of extracellular-signal related kinase 1/2 (ERK1/2) in the nucleus accumbens. We also report that aggressive interactions result in a transient decrease in the phosphorylation of mammalian target of rapamycin (mTOR) in the medial prefrontal cortex, a major input structure to the nucleus accumbens. Thus, this study identifies ERK1/2 and mTOR as potential signaling pathways for regulating the long-term rewarding consequences of aggressive interactions. Furthermore, the recruitment profile of the ERK1/2 and the mTOR pathways are distinct in different brain regions.
    MeSH term(s) Cricetinae ; Animals ; Female ; Nucleus Accumbens/metabolism ; Mesocricetus ; Phosphorylation ; MAP Kinase Signaling System ; Aggression/physiology ; Prefrontal Cortex/metabolism ; TOR Serine-Threonine Kinases/metabolism
    Chemical Substances TOR Serine-Threonine Kinases (EC 2.7.11.1)
    Language English
    Publishing date 2023-01-10
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24021379
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Report on the Malady at Walcheren.

    Borland, J / Lempriere, W / Blane, G

    The Medical and physical journal

    2018  Volume 23, Issue 133, Page(s) 183–187

    Language English
    Publishing date 2018-11-29
    Publishing country England
    Document type Journal Article
    ZDB-ID 2535269-6
    ISSN 0267-0100
    ISSN 0267-0100
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  6. Article: Stomatal Responses to Light, CO

    Santos, Mauro G / Davey, Phillip A / Hofmann, Tanja A / Borland, Anne / Hartwell, James / Lawson, Tracy

    Frontiers in plant science

    2021  Volume 12, Page(s) 740534

    Abstract: The responses of stomatal aperture to light intensity and ... ...

    Abstract The responses of stomatal aperture to light intensity and CO
    Language English
    Publishing date 2021-10-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2021.740534
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  7. Article ; Online: Trends in alcohol expenditure in Australia from 1984 to 2015-2016: An exploratory study.

    Smit, Koen / Dowling, Rowan / Livingston, Michael / Room, Robin / Laslett, Anne-Marie / Ferrier, Adamm / Livingstone, Charles / Borland, Ron / Jiang, Heng

    Drug and alcohol review

    2023  Volume 42, Issue 5, Page(s) 1235–1245

    Abstract: ... household expenditure was found across nearly all demographic groups (e.g., sex, age, employment, household income ...

    Abstract Introduction: Excessive alcohol use is associated with non-communicable diseases and social problems, such as work absence, financial problems and family violence. Expenditure and expenditure shares on alcohol are valuable measures to monitor financial activities on this risk behaviour. The aim of this paper is to report trends in alcohol expenditure in Australia over the last two decades.
    Methods: Data are from six waves of Australian Household Expenditure Surveys from 1984 to 2015-2016. We explored trends of alcohol expenditure among Australians and in different socio-demographic groups in the last 30 years. We further examined changes of expenditure on different on- and off-premises beverages over time.
    Results: Absolute alcohol expenditure has remained the same between the 1980s and 2016, after accounting for inflation. However, a declining trend in relative alcohol expenditure as a proportion of total household expenditure was found across nearly all demographic groups (e.g., sex, age, employment, household income), except for women aged 45-54, who showed an increasing trend of alcohol expenditure after 1998-1999.
    Discussion and conclusions: The current study shows declines in relative alcohol expenditure, which may reflect declines in alcohol's relative importance within the elements of the person's lifestyle they need to pay for and/or increased awareness of alcohol's health and social harms. Further longitudinal analysis should explore additional predictors of household expenditure on alcohol. Results suggest that current bi-annual indexation increases in alcohol tax should account for increases in income to ensure the effectiveness of pricing. Moreover, attention is needed to address drinking among middle-aged females.
    MeSH term(s) Middle Aged ; Humans ; Female ; Alcohol Drinking ; Health Expenditures ; Australia/epidemiology ; Alcoholic Beverages ; Ethanol
    Chemical Substances Ethanol (3K9958V90M)
    Language English
    Publishing date 2023-04-18
    Publishing country Australia
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1080442-0
    ISSN 1465-3362 ; 0959-5236
    ISSN (online) 1465-3362
    ISSN 0959-5236
    DOI 10.1111/dar.13662
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5621: Show issues Location:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article ; Online: Ontology-Based Interactive Visualization of Patient-Generated Research Questions.

    Borland, David / Christopherson, Laura / Schmitt, Charles

    Applied clinical informatics

    2019  Volume 10, Issue 3, Page(s) 377–386

    Abstract: ... of patients' lives (e.g., social interaction, family, work, diet, and sleep). Thus, treatment consists largely ...

    Abstract Background: Crohn's disease and colitis are chronic conditions that affect every facet of patients' lives (e.g., social interaction, family, work, diet, and sleep). Thus, treatment consists largely of disease management. The University of North Carolina at Chapel Hill chapter of the Crohn's and Colitis Foundation-IBD Partners-has created an interactive website that, in addition to providing helpful information and disease management tools, provides a discussion forum for patients to talk about their experiences and suggest new lines of research into Crohn's disease and colitis.
    Objectives: The primary objective of this work is to enable researchers to more effectively browse the forum content. Researchers wish to identify important/popular patient-suggested research topics, appreciate the full breadth of the research topics, and see connections between them, in order to more effectively prioritize research agendas.
    Methods: To help structure the forum content we have developed an ontology describing the major themes in the discussion forum. We have also created a prototype interactive visualization tool that leverages the ontology to help researchers identify common themes and related patient-generated research topics via linked views of (1) the ontology, (2) a research topic overview clustered by relevant ontology terms, and (3) a detailed view of the discussion forum content.
    Results: We discuss visualizations and interactions enabled by the visualization tool, provide an example scenario using the tool, and discuss limitations and future work based on feedback from potential users.
    Conclusion: The integration of a user-community specific ontology with an interactive visualization tool is a promising approach for enabling researchers to more effectively study user-generated research questions.
    MeSH term(s) Biological Ontologies ; Biomedical Research ; Colitis ; Crohn Disease ; Data Mining/methods ; Feedback ; Humans ; User-Computer Interface
    Language English
    Publishing date 2019-06-05
    Publishing country Germany
    Document type Journal Article
    ISSN 1869-0327
    ISSN (online) 1869-0327
    DOI 10.1055/s-0039-1688938
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  9. Article: Corrigendum: RNA Polymerase III, Ageing and Longevity.

    Kulaberoglu, Yavuz / Malik, Yasir / Borland, Gillian / Selman, Colin / Alic, Nazif / Tullet, Jennifer M A

    Frontiers in genetics

    2021  Volume 12, Page(s) 758135

    Abstract: This corrects the article DOI: 10.3389/fgene.2021.705122.]. ...

    Abstract [This corrects the article DOI: 10.3389/fgene.2021.705122.].
    Language English
    Publishing date 2021-09-02
    Publishing country Switzerland
    Document type Published Erratum
    ZDB-ID 2606823-0
    ISSN 1664-8021
    ISSN 1664-8021
    DOI 10.3389/fgene.2021.758135
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  10. Article: RNA Polymerase III, Ageing and Longevity.

    Kulaberoglu, Yavuz / Malik, Yasir / Borland, Gillian / Selman, Colin / Alic, Nazif / Tullet, Jennifer M A

    Frontiers in genetics

    2021  Volume 12, Page(s) 705122

    Abstract: Transcription in eukaryotic cells is performed by three RNA polymerases. RNA polymerase I synthesises most rRNAs, whilst RNA polymerase II transcribes all mRNAs and many non-coding RNAs. The largest of the three polymerases is RNA polymerase III (Pol III) ...

    Abstract Transcription in eukaryotic cells is performed by three RNA polymerases. RNA polymerase I synthesises most rRNAs, whilst RNA polymerase II transcribes all mRNAs and many non-coding RNAs. The largest of the three polymerases is RNA polymerase III (Pol III) which transcribes a variety of short non-coding RNAs including tRNAs and the 5S rRNA, in addition to other small RNAs such as snRNAs, snoRNAs, SINEs, 7SL RNA, Y RNA, and U6 spilceosomal RNA. Pol III-mediated transcription is highly dynamic and regulated in response to changes in cell growth, cell proliferation and stress. Pol III-generated transcripts are involved in a wide variety of cellular processes, including translation, genome and transcriptome regulation and RNA processing, with Pol III dys-regulation implicated in diseases including leukodystrophy, Alzheimer's, Fragile X-syndrome and various cancers. More recently, Pol III was identified as an evolutionarily conserved determinant of organismal lifespan acting downstream of mTORC1. Pol III inhibition extends lifespan in yeast, worms and flies, and in worms and flies acts from the intestine and intestinal stem cells respectively to achieve this. Intriguingly, Pol III activation achieved through impairment of its master repressor, Maf1, has also been shown to promote longevity in model organisms, including mice. In this review we introduce the Pol III transcription apparatus and review the current understanding of RNA Pol III's role in ageing and lifespan in different model organisms. We then discuss the potential of Pol III as a therapeutic target to improve age-related health in humans.
    Language English
    Publishing date 2021-07-06
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2606823-0
    ISSN 1664-8021
    ISSN 1664-8021
    DOI 10.3389/fgene.2021.705122
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