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  1. Article ; Online: From Catheter to Kidney Stone: The Uropathogenic Lifestyle of Proteus mirabilis.

    Norsworthy, Allison N / Pearson, Melanie M

    Trends in microbiology

    2017  Volume 25, Issue 4, Page(s) 304–315

    Abstract: Proteus mirabilis is a model organism for urease-producing uropathogens. These diverse bacteria cause infection stones in the urinary tract and form crystalline biofilms on indwelling urinary catheters, frequently leading to polymicrobial infection. ... ...

    Abstract Proteus mirabilis is a model organism for urease-producing uropathogens. These diverse bacteria cause infection stones in the urinary tract and form crystalline biofilms on indwelling urinary catheters, frequently leading to polymicrobial infection. Recent work has elucidated how P. mirabilis causes all of these disease states. Particularly exciting is the discovery that this bacterium forms large clusters in the bladder lumen that are sites for stone formation. These clusters, and other steps of infection, require two virulence factors in particular: urease and MR/P fimbriae. Highlighting the importance of MR/P fimbriae is the cotranscribed regulator, MrpJ, which globally controls virulence. Overall, P. mirabilis exhibits an extraordinary lifestyle, and further probing will answer exciting basic microbiological and clinically relevant questions.
    Language English
    Publishing date 2017-04
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1158963-2
    ISSN 1878-4380 ; 0966-842X
    ISSN (online) 1878-4380
    ISSN 0966-842X
    DOI 10.1016/j.tim.2016.11.015
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3738: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Signaling between two interacting sensor kinases promotes biofilms and colonization by a bacterial symbiont.

    Norsworthy, Allison N / Visick, Karen L

    Molecular microbiology

    2015  Volume 96, Issue 2, Page(s) 233–248

    Abstract: Cells acclimate to fluctuating environments by utilizing sensory circuits. One common sensory pathway used by bacteria is two-component signaling (TCS), composed of an environmental sensor [the sensor kinase (SK)] and a cognate, intracellular effector [ ... ...

    Abstract Cells acclimate to fluctuating environments by utilizing sensory circuits. One common sensory pathway used by bacteria is two-component signaling (TCS), composed of an environmental sensor [the sensor kinase (SK)] and a cognate, intracellular effector [the response regulator (RR)]. The squid symbiont Vibrio fischeri uses an elaborate TCS phosphorelay containing a hybrid SK, RscS, and two RRs, SypE and SypG, to control biofilm formation and host colonization. Here, we found that another hybrid SK, SypF, was essential for biofilms by functioning downstream of RscS to directly control SypE and SypG. Surprisingly, although wild-type SypF functioned as an SK in vitro, this activity was dispensable for colonization. In fact, only a single non-enzymatic domain within SypF, the HPt domain, was critical in vivo. Remarkably, this domain within SypF interacted with RscS to permit a bypass of RscS's own HPt domain and SypF's enzymatic function. This represents the first in vivo example of a functional SK that exploits the enzymatic activity of another SK, an adaptation that demonstrates the elegant plasticity in the arrangement of TCS regulators.
    MeSH term(s) Aliivibrio Infections/microbiology ; Aliivibrio Infections/veterinary ; Aliivibrio fischeri/enzymology ; Aliivibrio fischeri/genetics ; Aliivibrio fischeri/growth & development ; Aliivibrio fischeri/physiology ; Animals ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Biofilms ; Decapodiformes/microbiology ; Gene Expression Regulation, Bacterial ; Protein Kinases/genetics ; Protein Kinases/metabolism ; Signal Transduction ; Symbiosis
    Chemical Substances Bacterial Proteins ; Protein Kinases (EC 2.7.-)
    Language English
    Publishing date 2015-04
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 619315-8
    ISSN 1365-2958 ; 0950-382X
    ISSN (online) 1365-2958
    ISSN 0950-382X
    DOI 10.1111/mmi.12932
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2502: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: From Catheter to Kidney Stone: The Uropathogenic Lifestyle of Proteus mirabilis

    Norsworthy, Allison N / Melanie M. Pearson

    Trends in microbiology. 2017 Apr., v. 25, no. 4

    2017  

    Abstract: Proteus mirabilis is a model organism for urease-producing uropathogens. These diverse bacteria cause infection stones in the urinary tract and form crystalline biofilms on indwelling urinary catheters, frequently leading to polymicrobial infection. ... ...

    Abstract Proteus mirabilis is a model organism for urease-producing uropathogens. These diverse bacteria cause infection stones in the urinary tract and form crystalline biofilms on indwelling urinary catheters, frequently leading to polymicrobial infection. Recent work has elucidated how P. mirabilis causes all of these disease states. Particularly exciting is the discovery that this bacterium forms large clusters in the bladder lumen that are sites for stone formation. These clusters, and other steps of infection, require two virulence factors in particular: urease and MR/P fimbriae. Highlighting the importance of MR/P fimbriae is the cotranscribed regulator, MrpJ, which globally controls virulence. Overall, P. mirabilis exhibits an extraordinary lifestyle, and further probing will answer exciting basic microbiological and clinically relevant questions.
    Keywords bacteria ; biofilm ; bladder ; catheters ; fimbriae ; models ; Proteus mirabilis ; renal calculi ; urease ; virulence
    Language English
    Dates of publication 2017-04
    Size p. 304-315.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1158963-2
    ISSN 1878-4380 ; 0966-842X
    ISSN (online) 1878-4380
    ISSN 0966-842X
    DOI 10.1016/j.tim.2016.11.015
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 2005/714: Show issues

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  4. Article: Gimme shelter: how Vibrio fischeri successfully navigates an animal's multiple environments.

    Norsworthy, Allison N / Visick, Karen L

    Frontiers in microbiology

    2013  Volume 4, Page(s) 356

    Abstract: Bacteria successfully colonize distinct niches because they can sense and appropriately respond to a variety of environmental signals. Of particular interest is how a bacterium negotiates the multiple, complex environments posed during successful ... ...

    Abstract Bacteria successfully colonize distinct niches because they can sense and appropriately respond to a variety of environmental signals. Of particular interest is how a bacterium negotiates the multiple, complex environments posed during successful infection of an animal host. One tractable model system to study how a bacterium manages a host's multiple environments is the symbiotic relationship between the marine bacterium, Vibrio fischeri, and its squid host, Euprymna scolopes. V. fischeri encounters many different host surroundings ranging from initial contact with the squid to ultimate colonization of a specialized organ known as the light organ. For example, upon recognition of the squid, V. fischeri forms a biofilm aggregate outside the light organ that is required for efficient colonization. The bacteria then disperse from this biofilm to enter the organ, where they are exposed to nitric oxide, a molecule that can act as both a signal and an antimicrobial. After successfully managing this potentially hostile environment, V. fischeri cells finally establish their niche in the deep crypts of the light organ where the bacteria bioluminesce in a pheromone-dependent fashion, a phenotype that E. scolopes utilizes for anti-predation purposes. The mechanism by which V. fischeri manages these environments to outcompete all other bacterial species for colonization of E. scolopes is an important and intriguing question that will permit valuable insights into how a bacterium successfully associates with a host. This review focuses on specific molecular pathways that allow V. fischeri to establish this exquisite bacteria-host interaction.
    Language English
    Publishing date 2013-11-29
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2013.00356
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: MrpH, a new class of metal-binding adhesin, requires zinc to mediate biofilm formation.

    Jiang, Wangshu / Ubhayasekera, Wimal / Breed, Michael C / Norsworthy, Allison N / Serr, Nina / Mobley, Harry L T / Pearson, Melanie M / Knight, Stefan D

    PLoS pathogens

    2020  Volume 16, Issue 8, Page(s) e1008707

    Abstract: Proteus mirabilis, a Gram-negative uropathogen, is a major causative agent in catheter-associated urinary tract infections (CAUTI). Mannose-resistant Proteus-like fimbriae (MR/P) are crucially important for P. mirabilis infectivity and are required for ... ...

    Abstract Proteus mirabilis, a Gram-negative uropathogen, is a major causative agent in catheter-associated urinary tract infections (CAUTI). Mannose-resistant Proteus-like fimbriae (MR/P) are crucially important for P. mirabilis infectivity and are required for biofilm formation and auto-aggregation, as well as for bladder and kidney colonization. Here, the X-ray crystal structure of the MR/P tip adhesin, MrpH, is reported. The structure has a fold not previously described and contains a transition metal center with Zn2+ coordinated by three conserved histidine residues and a ligand. Using biofilm assays, chelation, metal complementation, and site-directed mutagenesis of the three histidines, we show that an intact metal binding site occupied by zinc is essential for MR/P fimbria-mediated biofilm formation, and furthermore, that P. mirabilis biofilm formation is reversible in a zinc-dependent manner. Zinc is also required for MR/P-dependent agglutination of erythrocytes, and mutation of the metal binding site renders P. mirabilis unfit in a mouse model of UTI. The studies presented here provide important clues as to the mechanism of MR/P-mediated biofilm formation and serve as a starting point for identifying the physiological MR/P fimbrial receptor.
    MeSH term(s) Adhesins, Bacterial/chemistry ; Adhesins, Bacterial/genetics ; Adhesins, Bacterial/metabolism ; Amino Acid Sequence ; Biofilms ; Fimbriae Proteins/chemistry ; Fimbriae Proteins/genetics ; Fimbriae Proteins/metabolism ; Humans ; Proteus Infections/metabolism ; Proteus Infections/microbiology ; Proteus mirabilis/chemistry ; Proteus mirabilis/genetics ; Proteus mirabilis/metabolism ; Sequence Alignment ; Urinary Tract Infections/metabolism ; Urinary Tract Infections/microbiology ; Zinc/chemistry ; Zinc/metabolism
    Chemical Substances Adhesins, Bacterial ; MrpH protein, Proteus mirabilis ; Fimbriae Proteins (147680-16-8) ; Zinc (J41CSQ7QDS)
    Language English
    Publishing date 2020-08-11
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7366
    ISSN (online) 1553-7374
    ISSN 1553-7366
    DOI 10.1371/journal.ppat.1008707
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Proteus mirabilis fimbriae- and urease-dependent clusters assemble in an extracellular niche to initiate bladder stone formation.

    Schaffer, Jessica N / Norsworthy, Allison N / Sun, Tung-Tien / Pearson, Melanie M

    Proceedings of the National Academy of Sciences of the United States of America

    2016  Volume 113, Issue 16, Page(s) 4494–4499

    Abstract: The catheter-associated uropathogenProteus mirabilisfrequently causes urinary stones, but little has been known about the initial stages of bladder colonization and stone formation. We found thatP. mirabilisrapidly invades the bladder urothelium, but ... ...

    Abstract The catheter-associated uropathogenProteus mirabilisfrequently causes urinary stones, but little has been known about the initial stages of bladder colonization and stone formation. We found thatP. mirabilisrapidly invades the bladder urothelium, but generally fails to establish an intracellular niche. Instead, it forms extracellular clusters in the bladder lumen, which form foci of mineral deposition consistent with development of urinary stones. These clusters elicit a robust neutrophil response, and we present evidence of neutrophil extracellular trap generation during experimental urinary tract infection. We identified two virulence factors required for cluster development: urease, which is required for urolithiasis, and mannose-resistantProteus-like fimbriae. The extracellular cluster formation byP. mirabilisstands in direct contrast to uropathogenicEscherichia coli, which readily formed intracellular bacterial communities but not luminal clusters or urinary stones. We propose that extracellular clusters are a key mechanism ofP. mirabilissurvival and virulence in the bladder.
    MeSH term(s) Animals ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Disease Models, Animal ; Female ; Fimbriae, Bacterial/genetics ; Fimbriae, Bacterial/metabolism ; Mice ; Mice, Inbred CBA ; Proteus Infections/genetics ; Proteus Infections/metabolism ; Proteus Infections/pathology ; Proteus mirabilis/genetics ; Proteus mirabilis/metabolism ; Proteus mirabilis/pathogenicity ; Urease/genetics ; Urease/metabolism ; Urinary Bladder/microbiology ; Urinary Bladder/pathology ; Urinary Bladder Calculi/genetics ; Urinary Bladder Calculi/metabolism ; Urinary Bladder Calculi/microbiology ; Urinary Bladder Calculi/pathology ; Uropathogenic Escherichia coli/genetics ; Uropathogenic Escherichia coli/metabolism ; Uropathogenic Escherichia coli/pathogenicity
    Chemical Substances Bacterial Proteins ; Urease (EC 3.5.1.5)
    Language English
    Publishing date 2016-04-04
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.1601720113
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1148: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  7. Article ; Online: MrpH, a new class of metal-binding adhesin, requires zinc to mediate biofilm formation.

    Wangshu Jiang / Wimal Ubhayasekera / Michael C Breed / Allison N Norsworthy / Nina Serr / Harry L T Mobley / Melanie M Pearson / Stefan D Knight

    PLoS Pathogens, Vol 16, Iss 8, p e

    2020  Volume 1008707

    Abstract: Proteus mirabilis, a Gram-negative uropathogen, is a major causative agent in catheter-associated urinary tract infections (CAUTI). Mannose-resistant Proteus-like fimbriae (MR/P) are crucially important for P. mirabilis infectivity and are required for ... ...

    Abstract Proteus mirabilis, a Gram-negative uropathogen, is a major causative agent in catheter-associated urinary tract infections (CAUTI). Mannose-resistant Proteus-like fimbriae (MR/P) are crucially important for P. mirabilis infectivity and are required for biofilm formation and auto-aggregation, as well as for bladder and kidney colonization. Here, the X-ray crystal structure of the MR/P tip adhesin, MrpH, is reported. The structure has a fold not previously described and contains a transition metal center with Zn2+ coordinated by three conserved histidine residues and a ligand. Using biofilm assays, chelation, metal complementation, and site-directed mutagenesis of the three histidines, we show that an intact metal binding site occupied by zinc is essential for MR/P fimbria-mediated biofilm formation, and furthermore, that P. mirabilis biofilm formation is reversible in a zinc-dependent manner. Zinc is also required for MR/P-dependent agglutination of erythrocytes, and mutation of the metal binding site renders P. mirabilis unfit in a mouse model of UTI. The studies presented here provide important clues as to the mechanism of MR/P-mediated biofilm formation and serve as a starting point for identifying the physiological MR/P fimbrial receptor.
    Keywords Immunologic diseases. Allergy ; RC581-607 ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2020-08-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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