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  1. Article ; Online: Morphological remodeling of

    Shepherd, Doulin C / Kaplan, Mohammed / Vankadari, Naveen / Kim, Ki Woo / Larson, Charles L / Dutka, Przemysław / Beare, Paul A / Krzymowski, Edward / Heinzen, Robert A / Jensen, Grant J / Ghosal, Debnath

    iScience

    2023  Volume 26, Issue 7, Page(s) 107210

    Abstract: ... Coxiella ... ...

    Abstract Coxiella burnetii
    Language English
    Publishing date 2023-06-24
    Publishing country United States
    Document type Journal Article
    ISSN 2589-0042
    ISSN (online) 2589-0042
    DOI 10.1016/j.isci.2023.107210
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Three-dimensional insights into human enveloped viruses in vitro and in situ.

    Vankadari, Naveen / Shepherd, Doulin C / Carter, Stephen D / Ghosal, Debnath

    Biochemical Society transactions

    2022  Volume 50, Issue 1, Page(s) 95–105

    Abstract: Viruses can be enveloped or non-enveloped, and require a host cell to replicate and package their genomes into new virions to infect new cells. To accomplish this task, viruses hijack the host-cell machinery to facilitate their replication by subverting ... ...

    Abstract Viruses can be enveloped or non-enveloped, and require a host cell to replicate and package their genomes into new virions to infect new cells. To accomplish this task, viruses hijack the host-cell machinery to facilitate their replication by subverting and manipulating normal host cell function. Enveloped viruses can have severe consequences for human health, causing various diseases such as acquired immunodeficiency syndrome (AIDS), seasonal influenza, COVID-19, and Ebola virus disease. The complex arrangement and pleomorphic architecture of many enveloped viruses pose a challenge for the more widely used structural biology techniques, such as X-ray crystallography. Cryo-electron tomography (cryo-ET), however, is a particularly well-suited tool for overcoming the limitations associated with visualizing the irregular shapes and morphology enveloped viruses possess at macromolecular resolution. The purpose of this review is to explore the latest structural insights that cryo-ET has revealed about enveloped viruses, with particular attention given to their architectures, mechanisms of entry, replication, assembly, maturation and egress during infection. Cryo-ET is unique in its ability to visualize cellular landscapes at 3-5 nanometer resolution. Therefore, it is the most suited technique to study asymmetric elements and structural rearrangements of enveloped viruses during infection in their native cellular context.
    MeSH term(s) Cryoelectron Microscopy ; Ebolavirus/metabolism ; Ebolavirus/ultrastructure ; Electron Microscope Tomography ; HIV-1/metabolism ; HIV-1/ultrastructure ; Herpesvirus 1, Human/metabolism ; Herpesvirus 1, Human/ultrastructure ; Humans ; SARS-CoV-2/metabolism ; SARS-CoV-2/ultrastructure ; Viruses/metabolism ; Viruses/ultrastructure
    Language English
    Publishing date 2022-02-15
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 184237-7
    ISSN 1470-8752 ; 0300-5127
    ISSN (online) 1470-8752
    ISSN 0300-5127
    DOI 10.1042/BST20210433
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: From cells to atoms: Cryo-EM as an essential tool to investigate pathogen biology, host-pathogen interaction, and drug discovery.

    Shepherd, Doulin C / Dalvi, Somavally / Ghosal, Debnath

    Molecular microbiology

    2021  Volume 117, Issue 3, Page(s) 610–617

    Abstract: Electron cryo-microscopy (cryo-EM) has lately emerged as a powerful method in structural biology and cell biology. While cryo-EM single-particle analysis (SPA) is now routinely delivering structures of purified proteins and protein complexes at near- ... ...

    Abstract Electron cryo-microscopy (cryo-EM) has lately emerged as a powerful method in structural biology and cell biology. While cryo-EM single-particle analysis (SPA) is now routinely delivering structures of purified proteins and protein complexes at near-atomic resolution, the use of electron cryo-tomography (cryo-ET), together with subtomogram averaging, is allowing visualization of macromolecular complexes in their native cellular environment, at unprecedented resolution. The unique ability of cryo-EM to provide information at many spatial resolution scales from ångströms to microns makes it an invaluable tool that bridges the classic "resolution-gap" between structural biology and cell biology domains. Like in many other fields of biology, in recent years, cryo-EM has revolutionized our understanding of pathogen biology, host-pathogen interaction and has made significant strides toward structure-based drug discovery. In a very recent example, during the ongoing coronavirus disease (COVID-19) pandemic, the structure of the stabilized severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein was deciphered by SPA. This led to the development of multiple vaccines. Alongside, cryo-ET provided key insights into the structure of the native virion, mechanism of its entry, replication, and budding; demonstrating the unrivaled power of cryo-EM in investigating pathogen biology, host-pathogen interaction, and drug discovery. In this review, we showcase a few examples of how different imaging modalities within cryo-EM have enabled the study of microbiology and host-pathogen interaction.
    MeSH term(s) Biology ; COVID-19 ; Cryoelectron Microscopy/methods ; Drug Discovery ; Host-Pathogen Interactions ; Humans ; SARS-CoV-2
    Language English
    Publishing date 2021-10-23
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 619315-8
    ISSN 1365-2958 ; 0950-382X
    ISSN (online) 1365-2958
    ISSN 0950-382X
    DOI 10.1111/mmi.14820
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2502: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

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  4. Article ; Online: Morphological remodeling of Coxiella burnetii during its biphasic developmental cycle revealed by cryo-electron tomography

    Doulin C. Shepherd / Mohammed Kaplan / Naveen Vankadari / Ki Woo Kim / Charles L. Larson / Przemysław Dutka / Paul A. Beare / Edward Krzymowski / Robert A. Heinzen / Grant J. Jensen / Debnath Ghosal

    iScience, Vol 26, Iss 7, Pp 107210- (2023)

    2023  

    Abstract: Summary: Coxiella burnetii is an obligate zoonotic bacterium that targets macrophages causing a disease called Q fever. It has a biphasic developmental life cycle where the extracellular and metabolically inactive small cell variant (SCV) transforms ... ...

    Abstract Summary: Coxiella burnetii is an obligate zoonotic bacterium that targets macrophages causing a disease called Q fever. It has a biphasic developmental life cycle where the extracellular and metabolically inactive small cell variant (SCV) transforms inside the host into the vegetative large cell variant (LCV). However, details about the morphological and structural changes of this transition are still lacking. Here, we used cryo-electron tomography to image both SCV and LCV variants grown either under axenic conditions or purified directly from host cells. We show that SCVs are characterized by equidistant stacks of inner membrane that presumably facilitate the transition to LCV, a transition coupled with the expression of the Dot/Icm type IVB secretion system (T4BSS). A class of T4BSS particles were associated with extracellular densities possibly involved in host infection. Also, SCVs contained spherical multilayered membrane structures of different sizes and locations suggesting no connection to sporulation as once assumed.
    Keywords Microbiology ; Structural biology ; Science ; Q
    Language English
    Publishing date 2023-07-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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