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Article ; Online: In vivo characterization of laser-assisted delivery of hyaluronic acid using multiphoton fluorescence lifetime imaging.

Nguyen, Lynhda / Mess, Christian / Schneider, Stefan W / Huck, Volker / Herberger, Katharina

2023 Volume 32, Issue 12, Page(s) 2131–2137

Abstract: Laser-assisted drug delivery (LADD) is a treatment method to enhance the penetration of pharmaceuticals through the skin. The aim of the present study is to track hyaluronic acid (HA) and analyse its effect on human skin in vivo after ablative fractional ...

Abstract	Laser-assisted drug delivery (LADD) is a treatment method to enhance the penetration of pharmaceuticals through the skin. The aim of the present study is to track hyaluronic acid (HA) and analyse its effect on human skin in vivo after ablative fractional laser (AFL) treatment. Healthy male and female subjects were recruited. Four areas were marked on their forearms of each volunteer, and each area was assigned to one of the following treatment options: AFL + HA, AFL only, HA only or untreated control. A carbon dioxide laser was used for the AFL treatment. Follow-up measurements were scheduled 30 min and 30 days after treatment using multiphoton tomography equipped with fluorescence lifetime imaging (MPT-FLIM). A total of 11 subjects completed the study. By detecting fluorescence lifetimes, the HA and the anaesthetic ointment were clearly distinguishable from surrounding tissue. After AFL treatment, HA could be visualized in all epidermal and upper dermal layers. In contrast, HA in intact skin was only detected in the superficial layers at distinctly lower levels. The applied HA gel seemed to have beneficial properties for the wound healing process after laser treatment. LADD has proven to be a fast and effective method to increase HA uptake into the skin, allowing for improved hydration and skin rejuvenation over time. Furthermore, LADD could be a beneficial treatment option in laser resurfacing. MPT-FLIM proved to be an appropriate diagnostic tool for drug delivery tracking and monitoring of treatment response for individualized therapy adjustment.
MeSH term(s)	Humans ; Male ; Female ; Hyaluronic Acid/pharmacology ; Fluorescence ; Skin/diagnostic imaging ; Wound Healing ; Lasers, Gas/therapeutic use
Chemical Substances	Hyaluronic Acid (9004-61-9)
Language	English
Publishing date	2023-10-17
Publishing country	Denmark
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1130936-2
ISSN	1600-0625 ; 0906-6705
ISSN (online)	1600-0625
ISSN	0906-6705
DOI	10.1111/exd.14961
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: In vivo visualisation of tattoo particles using multiphoton tomography and fluorescence lifetime imaging.

Nguyen, Lynhda / Mess, Christian / Schneider, Stefan W / Huck, Volker / Herberger, Katharina

Experimental dermatology

2022 Volume 31, Issue 11, Page(s) 1712–1719

Abstract: With increased popularity of decorative tattoos, awareness of tattoo-based dermatological complications has been raised. Health issues include a broad spectrum dominated by allergies and infections. To examine the etiopathology and prognose the outcome ... ...

Abstract	With increased popularity of decorative tattoos, awareness of tattoo-based dermatological complications has been raised. Health issues include a broad spectrum dominated by allergies and infections. To examine the etiopathology and prognose the outcome of an appropriate therapy, a non-invasive intravital diagnostic approach is indicated. The present pilot study introduces multiphoton tomography equipped with fluorescence lifetime imaging as a diagnostic technique to examine the morphological and metabolic status of tattooed human skin at patient's bedside. The distributing course of tattoo particles can be visualised over time. By providing optical biopsies, inflammation-based alterations in freshly tattooed skin and tattoo complications can be analysed. The study concludes that multiphoton tomography combined with fluorescence lifetime imaging is a suitable technique for in vivo visualisation of tattoo pigments as well as for the assessment of quantitative and qualitative skin changes after injection of tattoo ink into human skin.
MeSH term(s)	Humans ; Tattooing ; Pilot Projects ; Coloring Agents ; Color ; Ink ; Tomography
Chemical Substances	Coloring Agents
Language	English
Publishing date	2022-07-25
Publishing country	Denmark
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1130936-2
ISSN	1600-0625 ; 0906-6705
ISSN (online)	1600-0625
ISSN	0906-6705
DOI	10.1111/exd.14646
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Biological and psychosocial factors associated with the persistence of pruritus symptoms: protocol for a prospective, exploratory observational study in Germany (individual project of the Interdisciplinary SOMACROSS Research Unit [RU 5211]).

Schneider, Gudrun / Ständer, Sonja / Kahnert, Stefan / Pereira, Manuel Pedro / Mess, Christian / Huck, Volker / Agelopoulos, Konstantin / Frank, Gina / Schneider, Stefan W

BMJ open

2022 Volume 12, Issue 7, Page(s) e060811

Abstract: Introduction: Chronic pruritus (CP) is a symptom of dermatologic, neurologic, systemic and psychosomatic diseases. CP has a prevalence of ~20% in the general population and is therefore a significant burden on society, but the transition from acute ... ...

Abstract	Introduction: Chronic pruritus (CP) is a symptom of dermatologic, neurologic, systemic and psychosomatic diseases. CP has a prevalence of ~20% in the general population and is therefore a significant burden on society, but the transition from acute pruritus to CP is not well understood. It probably involves interactions between biological and psychosocial factors and pruritus-specific risk factors as well as mechanisms shared with other persistent somatic symptoms addressed in other projects of the SOMACROSS Research Unit (RU). Here we aim to identify psychosocial and biological factors and their interactions which might be associated with the persistence of CP with and without immunologic/inflammatory origin, that is, atopic dermatitis and pruritus on non-inflamed skin. We expect that psychosocial factors relevant to the persistence of symptoms such as fatigue and pain may also show associations to CP. Methods and analysis: In this prospective, exploratory observational study situated in Germany, three cohorts of 40 patients each with acute exacerbation of atopic dermatitis and chronic atopic dermatitis and 40 CP patients with unaffected skin will be recruited for a comprehensive translational investigation including pruritus-specific and the shared psychosocial assessments of the RU SOMACROSS. Pruritus-specific measures will include questionnaires, quantitative sensory testing, cutaneous nerve fibre morphology, skin barrier morphology, epidermal metabolism and pruritogen blood levels. Within 1 year, patients and 80 age-matched and sex-matched healthy controls will be examined at three time points, allowing cross-sectional comparison and a longitudinal investigation of predictive outcome factors in patients under treatment according to existing guidelines. Ethics and dissemination: The study has been approved by the ethics committees of Hamburg (2020-10200-BO-ff) and Münster (2020-676 f-S), Germany. All participants are required to provide written informed consent. Findings will be disseminated through peer-reviewed publications, scientific conferences and involvement of relevant stakeholders, patients and the lay public. Trial registration number: DRKS00026646.
MeSH term(s)	Cross-Sectional Studies ; Dermatitis, Atopic/complications ; Germany/epidemiology ; Humans ; Observational Studies as Topic ; Prospective Studies ; Pruritus/epidemiology
Language	English
Publishing date	2022-07-07
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2599832-8
ISSN	2044-6055 ; 2044-6055
ISSN (online)	2044-6055
ISSN	2044-6055
DOI	10.1136/bmjopen-2022-060811
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Skin Barriers in Dermal Drug Delivery: Which Barriers Have to Be Overcome and How Can We Measure Them?

Gorzelanny, Christian / Mess, Christian / Schneider, Stefan W / Huck, Volker / Brandner, Johanna M

Pharmaceutics

2020 Volume 12, Issue 7

Abstract: Although, drugs are required in the various skin compartments such as viable epidermis, dermis, or hair follicles, to efficiently treat skin diseases, drug delivery into and across the skin is still challenging. An improved understanding of skin barrier ... ...

Abstract	Although, drugs are required in the various skin compartments such as viable epidermis, dermis, or hair follicles, to efficiently treat skin diseases, drug delivery into and across the skin is still challenging. An improved understanding of skin barrier physiology is mandatory to optimize drug penetration and permeation. The various barriers of the skin have to be known in detail, which means methods are needed to measure their functionality and outside-in or inside-out passage of molecules through the various barriers. In this review, we summarize our current knowledge about mechanical barriers, i.e., stratum corneum and tight junctions, in interfollicular epidermis, hair follicles and glands. Furthermore, we discuss the barrier properties of the basement membrane and dermal blood vessels. Barrier alterations found in skin of patients with atopic dermatitis are described. Finally, we critically compare the up-to-date applicability of several physical, biochemical and microscopic methods such as transepidermal water loss, impedance spectroscopy, Raman spectroscopy, immunohistochemical stainings, optical coherence microscopy and multiphoton microscopy to distinctly address the different barriers and to measure permeation through these barriers in vitro and in vivo.
Language	English
Publishing date	2020-07-20
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2527217-2
ISSN	1999-4923
ISSN	1999-4923
DOI	10.3390/pharmaceutics12070684
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Biological and psychosocial factors associated with the persistence of pruritus symptoms

Sonja Ständer / Stefan W Schneider / Gudrun Schneider / Stefan Kahnert / Manuel Pedro Pereira / Christian Mess / Volker Huck / Konstantin Agelopoulos / Gina Frank

BMJ Open, Vol 12, Iss

protocol for a prospective, exploratory observational study in Germany (individual project of the Interdisciplinary SOMACROSS Research Unit [RU 5211])

2022 Volume 7

Abstract: Introduction Chronic pruritus (CP) is a symptom of dermatologic, neurologic, systemic and psychosomatic diseases. CP has a prevalence of ~20% in the general population and is therefore a significant burden on society, but the transition from acute ... ...

Abstract	Introduction Chronic pruritus (CP) is a symptom of dermatologic, neurologic, systemic and psychosomatic diseases. CP has a prevalence of ~20% in the general population and is therefore a significant burden on society, but the transition from acute pruritus to CP is not well understood. It probably involves interactions between biological and psychosocial factors and pruritus-specific risk factors as well as mechanisms shared with other persistent somatic symptoms addressed in other projects of the SOMACROSS Research Unit (RU). Here we aim to identify psychosocial and biological factors and their interactions which might be associated with the persistence of CP with and without immunologic/inflammatory origin, that is, atopic dermatitis and pruritus on non-inflamed skin. We expect that psychosocial factors relevant to the persistence of symptoms such as fatigue and pain may also show associations to CP.Methods and analysis In this prospective, exploratory observational study situated in Germany, three cohorts of 40 patients each with acute exacerbation of atopic dermatitis and chronic atopic dermatitis and 40 CP patients with unaffected skin will be recruited for a comprehensive translational investigation including pruritus-specific and the shared psychosocial assessments of the RU SOMACROSS. Pruritus-specific measures will include questionnaires, quantitative sensory testing, cutaneous nerve fibre morphology, skin barrier morphology, epidermal metabolism and pruritogen blood levels. Within 1 year, patients and 80 age-matched and sex-matched healthy controls will be examined at three time points, allowing cross-sectional comparison and a longitudinal investigation of predictive outcome factors in patients under treatment according to existing guidelines.Ethics and dissemination The study has been approved by the ethics committees of Hamburg (2020-10200-BO-ff) and Münster (2020-676 f-S), Germany. All participants are required to provide written informed consent. Findings will be disseminated through peer-reviewed ...
Keywords	Medicine ; R
Subject code	610
Language	English
Publishing date	2022-07-01T00:00:00Z
Publisher	BMJ Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: International organizations and the private sector

Huck, Volker / Schomaker, Rahel

Internationale Organisationen : ordnungspolitische Grundlagen, Perspektiven und Anwendungsbereiche , p. 233-253

global public-private partnerships for global public goods?

2013 , Page(s) 233–253

Author's details	Volker Huck and Rahel Schomaker
Keywords	Öffentlich-private Partnerschaft ; Internationale Organisation ; Gesundheitspolitik ; Welt
Language	English
Size	graph. Darst.
Publisher	Lucius & Lucius
Publishing place	Stuttgart
Document type	Article
ISBN	978-3-8282-0579-6 ; 3-8282-0579-8
Database	ECONomics Information System

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Article ; Conference proceedings: Functional and Structural Properties of the 2nd Gain-of-Function Variant in the C4 Domain of von Willebrand Factor

Brehm, Maria A. / König, Gesa / Obser, Tobias / Huck, Volker / Schneider, Stefan W. / Schneppenheim, Reinhard

Hämostaseologie

2020 Volume 40, Issue S 01

Event/congress	Hamburger Hämophilie Symposion Hamburg, Germany, Germany, 2019-11-08
Language	English
Publishing date	2020-11-01
Publisher	Georg Thieme Verlag KG
Publishing place	Stuttgart ; New York
Document type	Article ; Conference proceedings
ZDB-ID	801512-0
ISSN	2567-5761 ; 0720-9355
ISSN (online)	2567-5761
ISSN	0720-9355
DOI	10.1055/s-0040-1721606
Database	Thieme publisher's database

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Zs.A 1631: Show issues

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Article ; Online: Platelet adhesion and aggregate formation controlled by immobilised and soluble VWF.

Schneider, Matthias F / Fallah, Mohammad A / Mess, Christian / Obser, Tobias / Schneppenheim, Reinhard / Alexander-Katz, Alfredo / Schneider, Stefan W / Huck, Volker

BMC molecular and cell biology

2020 Volume 21, Issue 1, Page(s) 64

Abstract: Background: It has been demonstrated that von Willebrand factor (VWF) mediated platelet-endothelium and platelet-platelet interactions are shear dependent. The VWF's mobility under dynamic conditions (e.g. flow) is pivotal to platelet adhesion and VWF- ... ...

Abstract	Background: It has been demonstrated that von Willebrand factor (VWF) mediated platelet-endothelium and platelet-platelet interactions are shear dependent. The VWF's mobility under dynamic conditions (e.g. flow) is pivotal to platelet adhesion and VWF-mediated aggregate formation in the cascade of VWF-platelet interactions in haemostasis. Results: Combining microfluidic tools with fluorescence and reflection interference contrast microscopy (RICM), here we show, that specific deletions in the A-domains of the biopolymer VWF affect both, adhesion and aggregation properties independently. Intuitively, the deletion of the A1-domain led to a significant decrease in both adhesion and aggregate formation of platelets. Nevertheless, the deletion of the A2-domain revealed a completely different picture, with a significant increase in formation of rolling aggregates (gain of function). We predict that the A2-domain effectively 'masks' the potential between the platelet glycoprotein (GP) Ib and the VWF A1-domain. Furthermore, the deletion of the A3-domain led to no significant variation in either of the two functional characteristics. Conclusions: These data demonstrate that the macroscopic functional properties i.e. adhesion and aggregate formation cannot simply be assigned to the properties of one particular domain, but have to be explained by cooperative phenomena. The absence or presence of molecular entities likewise affects the properties (thermodynamic phenomenology) of its neighbours, therefore altering the macromolecular function.
Language	English
Publishing date	2020-09-11
Publishing country	England
Document type	Journal Article
ISSN	2661-8850
ISSN (online)	2661-8850
DOI	10.1186/s12860-020-00309-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Biological and psychosocial factors associated with the persistence of pruritus symptoms

Schneider, Gudrun / Ständer, Sonja / Kahnert, Stefan / Pereira, Manuel Pedro / Mess, Christian / Huck, Volker / Agelopoulos, Konstantin / Frank, Gina / Schneider, Stefan W.

BMJ Open

Protocol for a prospective, exploratory observational study in Germany (individual project of the Interdisciplinary SOMACROSS Research Unit [RU 5211])

2022

Abstract: Introduction: Chronic pruritus (CP) is a symptom of dermatologic, neurologic, systemic and psychosomatic diseases. CP has a prevalence of similar to 20% in the general population and is therefore a significant burden on society, but the transition from ... ...

Title translation	Biologische und psychosoziale Faktoren im Zusammenhang mit der Persistenz von Juckreizsymptomen: Protokoll für eine prospektive, explorative Beobachtungsstudie in Deutschland (Einzelprojekt der Interdisziplinären Forschungseinheit SOMACROSS [RU 5211])
Abstract	Introduction: Chronic pruritus (CP) is a symptom of dermatologic, neurologic, systemic and psychosomatic diseases. CP has a prevalence of similar to 20% in the general population and is therefore a significant burden on society, but the transition from acute pruritus to CP is not well understood. It probably involves interactions between biological and psychosocial factors and pruritus-specific risk factors as well as mechanisms shared with other persistent somatic symptoms addressed in other projects of the SOMACROSS Research Unit (RU). Here we aim to identify psychosocial and biological factors and their interactions which might be associated with the persistence of CP with and without immunologic/inflammatory origin, that is, atopic dermatitis and pruritus on non-inflamed skin. We expect that psychosocial factors relevant to the persistence of symptoms such as fatigue and pain may also show associations to CP. Methods and analysis: In this prospective, exploratory observational study situated in Germany, three cohorts of 40 patients each with acute exacerbation of atopic dermatitis and chronic atopic dermatitis and 40 CP patients with unaffected skin will be recruited for a comprehensive translational investigation including pruritus-specific and the shared psychosocial assessments of the RU SOMACROSS. Pruritus-specific measures will include questionnaires, quantitative sensory testing, cutaneous nerve fibre morphology, skin barrier morphology, epidermal metabolism and pruritogen blood levels. Within 1 year, patients and 80 age-matched and sex-matched healthy controls will be examined at three time points, allowing cross-sectional comparison and a longitudinal investigation of predictive outcome factors in patients under treatment according to existing guidelines. Ethics and dissemination The study has been approved: by the ethics committees of Hamburg (2020-10200-BO-ff) and Munster (2020-676 f-S), Germany. All participants are required to provide written informed consent. Findings will be disseminated through peer-reviewed publications, scientific conferences and involvement of relevant stakeholders, patients and the lay public.
Keywords	Biological Markers ; Biologische Marker ; Chronic Illness ; Chronicity (Disorders) ; Chronische Krankheit ; Chronischer Verlauf von Krankheiten ; Hautkrankheiten ; Pruritus ; Psychosocial Factors ; Psychosoziale Faktoren ; Risikofaktoren ; Risk Factors ; Skin Disorders
Language	English
Document type	Article
DOI	10.1136/bmjopen-2022-060811
Database	PSYNDEX

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Article ; Online: DNA binds to a specific site of the adhesive blood-protein von Willebrand factor guided by electrostatic interactions.

Sandoval-Pérez, Angélica / Berger, Ricarda M L / Garaizar, Adiran / Farr, Stephen E / Brehm, Maria A / König, Gesa / Schneider, Stefan W / Collepardo-Guevara, Rosana / Huck, Volker / Rädler, Joachim O / Aponte-Santamaría, Camilo

Nucleic acids research

2020 Volume 48, Issue 20, Page(s) 11812–11813

Language	English
Publishing date	2020-10-22
Publishing country	England
Document type	Journal Article ; Published Erratum
ZDB-ID	186809-3
ISSN	1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048
ISSN (online)	1362-4962 ; 1362-4954
ISSN	0301-5610 ; 0305-1048
DOI	10.1093/nar/gkaa988
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