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  1. Article ; Online: Establishment of loop-mediated isothermal amplification for

    Yin-Bo, Ye / Dong-Liang, Li / Bo-Han, Yang / Zhao, Zhang / Si-Yao, Mei / Bao-Shan, Liu / Ze-Liang, Chen

    BioTechniques

    2022  Volume 73, Issue 3, Page(s) 142–150

    Abstract: The study sought to establish a sensitive and specific on-site loop-mediated isothermal amplification (LAMP) ... ...

    Abstract The study sought to establish a sensitive and specific on-site loop-mediated isothermal amplification (LAMP) for
    MeSH term(s) Brucella/genetics ; Heating ; Molecular Diagnostic Techniques ; Nucleic Acid Amplification Techniques/methods ; Sensitivity and Specificity
    Language English
    Publishing date 2022-08-23
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 48453-2
    ISSN 1940-9818 ; 0736-6205
    ISSN (online) 1940-9818
    ISSN 0736-6205
    DOI 10.2144/btn-2021-0043
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2435: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Cascade Multicomponent Assemblies Involving 1,3-Enynes via Auto-Tandem Palladium Catalysis.

    He, Ze-Liang / Zhang, Yi / Chen, Zhi-Chao / Du, Wei / Chen, Ying-Chun

    Organic letters

    2022  Volume 24, Issue 34, Page(s) 6326–6330

    Abstract: Here we report a three-component auto-tandem reaction of 1,3-enyne-tethered carbonyls, organoboronic reagents, and suitable nucleophiles catalyzed by palladium, proceeding through consecutive intramolecular vinylogous addition, Suzuki coupling, and ... ...

    Abstract Here we report a three-component auto-tandem reaction of 1,3-enyne-tethered carbonyls, organoboronic reagents, and suitable nucleophiles catalyzed by palladium, proceeding through consecutive intramolecular vinylogous addition, Suzuki coupling, and allylic alkylation. This process exhibited high chemo- and regioselectivity with 1,3,4-trifunctionalization of the 1,3-enyne motif, and a wide range of 2
    MeSH term(s) Alkylation ; Catalysis ; Indoles ; Molecular Structure ; Palladium
    Chemical Substances Indoles ; Palladium (5TWQ1V240M)
    Language English
    Publishing date 2022-08-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1523-7052
    ISSN (online) 1523-7052
    DOI 10.1021/acs.orglett.2c02544
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Construction of Hydrodibenzo[

    He, Ze-Liang / Chen, Peng / Chen, Zhi-Chao / Du, Wei / Chen, Ying-Chun

    Organic letters

    2021  Volume 24, Issue 1, Page(s) 100–104

    Abstract: A cascade assembly between isatin-derived Morita-Baylis-Hillman carbonates ... ...

    Abstract A cascade assembly between isatin-derived Morita-Baylis-Hillman carbonates and
    Language English
    Publishing date 2021-12-10
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1523-7052
    ISSN (online) 1523-7052
    DOI 10.1021/acs.orglett.1c03689
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  4. Article: Cascade Multicomponent Assemblies Involving 1,3-Enynes via Auto-Tandem Palladium Catalysis

    He, Ze-Liang / Zhang, Yi / Chen, Zhi-Chao / Du, Wei / Chen, Ying-Chun

    Organic letters. 2022 Aug. 23, v. 24, no. 34

    2022  

    Abstract: Here we report a three-component auto-tandem reaction of 1,3-enyne-tethered carbonyls, organoboronic reagents, and suitable nucleophiles catalyzed by palladium, proceeding through consecutive intramolecular vinylogous addition, Suzuki coupling, and ... ...

    Abstract Here we report a three-component auto-tandem reaction of 1,3-enyne-tethered carbonyls, organoboronic reagents, and suitable nucleophiles catalyzed by palladium, proceeding through consecutive intramolecular vinylogous addition, Suzuki coupling, and allylic alkylation. This process exhibited high chemo- and regioselectivity with 1,3,4-trifunctionalization of the 1,3-enyne motif, and a wide range of 2H-chromenes, 1,2-dihydroquinolines, benzo[b]oxepines, 1,7-annulated indoles, and other frameworks were efficiently constructed in fair to good yields and E/Z selectivity.
    Keywords Lewis bases ; alkylation ; catalytic activity ; indoles ; palladium ; regioselectivity
    Language English
    Dates of publication 2022-0823
    Size p. 6326-6330.
    Publishing place American Chemical Society
    Document type Article
    ISSN 1523-7052
    DOI 10.1021/acs.orglett.2c02544
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  5. Article ; Online: DNA Extraction with TRIzol Reagent Using a Silica Column.

    Yang, Bo-Han / Liu, Bao-Shan / Chen, Ze-Liang

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

    2020  Volume 37, Issue 7, Page(s) 1033–1037

    Abstract: TRIzol is a monophasic solution of phenol and guanidine isothiocyanate used for the extraction of RNA, DNA and proteins from tissues or cells. However, few studies have described its application to DNA extraction due to its time-consuming procedure. We ... ...

    Abstract TRIzol is a monophasic solution of phenol and guanidine isothiocyanate used for the extraction of RNA, DNA and proteins from tissues or cells. However, few studies have described its application to DNA extraction due to its time-consuming procedure. We present a TRIzol-modified method of extracting DNA from tissues using the TRIzol reagent and a silica column, which requires only one-third of the time required for the classic extraction procedure. Spectrophotometric analysis showed that the 260/280 and 260/230 nm optical density ratio of the DNA extracted using the TRIzol-modified method is ideal and equal to that obtained by the classic method and commercial DNAiso methods. The DNA extracted by the TRIzol-modified method had the same performance in a restriction enzyme digestion and quantitative PCR as that extracted using the classic method. Using the TRIzol-modified method saves time, simplifies the DNA extraction procedure, and facilitates various molecular biology assays.
    MeSH term(s) DNA/genetics ; Guanidines ; Indicators and Reagents ; Phenols ; Silicon Dioxide
    Chemical Substances Guanidines ; Indicators and Reagents ; Phenols ; trizol ; Silicon Dioxide (7631-86-9) ; DNA (9007-49-2)
    Language English
    Publishing date 2020-11-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1483376-1
    ISSN 1348-2246 ; 1348-2246
    ISSN (online) 1348-2246
    ISSN 1348-2246
    DOI 10.2116/analsci.20P361
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Construction of Hydrodibenzo[b,d]furan Frameworks from Morita–Baylis–Hillman Carbonates of Isatins and o-Hydroxy Enones via Palladium and Brønsted Base Relay Catalysis

    He, Ze-Liang / Chen, Peng / Chen, Zhi-Chao / Du, Wei / Chen, Ying-Chun

    Organic letters. 2021 Dec. 10, v. 24, no. 1

    2021  

    Abstract: A cascade assembly between isatin-derived Morita–Baylis–Hillman carbonates and o-hydroxybenzylideneacetones has been developed under the relay catalysis of Pd(PPh₃)₄ and DBU, affording a spectrum of 1,2,3,4-tetrahydrodibenzo[b,d]furan architectures ... ...

    Abstract A cascade assembly between isatin-derived Morita–Baylis–Hillman carbonates and o-hydroxybenzylideneacetones has been developed under the relay catalysis of Pd(PPh₃)₄ and DBU, affording a spectrum of 1,2,3,4-tetrahydrodibenzo[b,d]furan architectures incorporating a spirooxindole motif in moderate to good yields with excellent diastereoselectivity. The fused indole analogues were similarly furnished by employing the benzylideneacetones having an o-TsNH group.
    Keywords catalytic activity ; diastereoselectivity ; furans ; indoles ; palladium
    Language English
    Dates of publication 2021-1210
    Size p. 100-104.
    Publishing place American Chemical Society
    Document type Article
    ISSN 1523-7052
    DOI 10.1021/acs.orglett.1c03689
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Combined immunization with inactivated vaccine reduces the dose of live B. abortus A19 vaccine.

    He, Chuan-Yu / Zhang, Yu-Zhuo / Liu, Meng-Zhi / Zhao, Hai-Long / Ren, Li-Song / Liu, Bao-Shan / He, Sun / Chen, Ze-Liang

    BMC veterinary research

    2022  Volume 18, Issue 1, Page(s) 128

    Abstract: Background: Brucella spp. is an important zoonotic pathogen responsible for brucellosis in humans and animals. Brucella abortus A19 strain is a widespread vaccine in China. However, it has a drawback of residual virulence in animals and humans.: ... ...

    Abstract Background: Brucella spp. is an important zoonotic pathogen responsible for brucellosis in humans and animals. Brucella abortus A19 strain is a widespread vaccine in China. However, it has a drawback of residual virulence in animals and humans.
    Methods: In this study, the BALB/c mice were inoculated with either 100 μL PBS(control group, C group), 10
    Results: The splenic bacterial burden and spleen weight of the mice in group LI were mostly equivalent to the mice of group H. Moreover, Brucella-specific serum IgG, IFN-γ, IL-4, and the percentage of CD4
    Conclusions: Combined immunization with low dose live vaccine and inactivated vaccine allowed to reduce the live B. abortus A19 vaccine, dose with an equivalent protection of the high-dose live vaccine.
    MeSH term(s) Animals ; Brucella Vaccine ; CD8-Positive T-Lymphocytes ; Immunization/veterinary ; Mice ; Vaccination/veterinary ; Vaccines, Inactivated
    Chemical Substances Brucella Vaccine ; Vaccines, Inactivated
    Language English
    Publishing date 2022-04-02
    Publishing country England
    Document type Journal Article
    ZDB-ID 2191675-5
    ISSN 1746-6148 ; 1746-6148
    ISSN (online) 1746-6148
    ISSN 1746-6148
    DOI 10.1186/s12917-022-03229-0
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  8. Article: Development and validation of a nomogram for Siewert II esophagogastric junction adenocarcinoma: a retrospective analysis.

    Jin, Tao / Li, Ze-Dong / Chen, Ze-Hua / He, Feng-Jun / Chen, Zheng-Wen / Liang, Pan-Ping / Hu, Jian-Kun / Yang, Kun

    Therapeutic advances in medical oncology

    2024  Volume 16, Page(s) 17588359241229425

    Abstract: Background: Due to the complex histological type and anatomical structures, there has been considerable debate on the classification of adenocarcinoma of the esophagogastric junction (AEG), especially Siewert II AEG. Furthermore, neither the American ... ...

    Abstract Background: Due to the complex histological type and anatomical structures, there has been considerable debate on the classification of adenocarcinoma of the esophagogastric junction (AEG), especially Siewert II AEG. Furthermore, neither the American Joint Committee on Cancer (AJCC) 7th tumor-node-metastasis (TNM) [esophageal adenocarcinoma (E) or gastric cancer (G)] nor the AJCC 8th TNM (E or G) accurately predicted the prognosis of patients with Siewert II AEG.
    Objective: This study aimed to investigate the factors influencing the survival and prognosis of patients with Siewert II AEG and establish a new and better prognostic predictive model.
    Design: A retrospective study.
    Methods: Patients with Siewert II AEG, retrieved from the Surveillance, Epidemiology, and End Results (SEER) databases, were assigned to the training set. Patients retrieved from a single tertiary medical center were assigned to the external validation set. Significant variables were selected using univariate and multivariate Cox regression analyses to construct the nomogram. Nomogram models were assessed using the concordance index (C-index), a calibration plot, decision curve analysis (DCA), and external validation.
    Results: Age, tumor grade, and size, as well as the T, N, and M stages, were included in the nomograms. For the SEER training set, the C-index of the nomogram was 0.683 (0.665-0.701). The C-index of the nomogram for the external validation set was 0.690 (0.653-0.727). The calibration curve showed good agreement between the nomogram estimations and actual observations in both the training and external validation sets. The DCA showed that the nomogram was clinically useful.
    Conclusion: The new predictive model showed significant accuracy in predicting the prognosis of Siewert II AEG.
    Language English
    Publishing date 2024-02-04
    Publishing country England
    Document type Journal Article
    ZDB-ID 2503443-1
    ISSN 1758-8359 ; 1758-8340
    ISSN (online) 1758-8359
    ISSN 1758-8340
    DOI 10.1177/17588359241229425
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: First Report of Tomato spotted wilt virus infecting

    Zhang, Sha-Sha / Yuan, Chen-Qing / Liang, Yan / Shen, Chu-Ze / Li, Li-Fang

    Plant disease

    2023  

    Abstract: ... ...

    Abstract Strawflower
    Language English
    Publishing date 2023-06-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 754182-x
    ISSN 0191-2917
    ISSN 0191-2917
    DOI 10.1094/PDIS-04-23-0683-PDN
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 2043: Show issues

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  10. Article: A specific reverse complement sequence for distinguishing

    Ye, Yin-Bo / Yang, Jiang-Hua / Li, Dong-Liang / Hao, Li-Hua / Zhang, Zhao / Mei, Si-Yao / Zhang, Huan / Du, Fang-Yuan / Yv, Li-Hui / Liu, Bao-Shan / Chen, Ze-Liang

    Frontiers in veterinary science

    2022  Volume 9, Page(s) 983482

    Abstract: Canine brucellosis is primarily caused ... ...

    Abstract Canine brucellosis is primarily caused by
    Language English
    Publishing date 2022-11-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2834243-4
    ISSN 2297-1769
    ISSN 2297-1769
    DOI 10.3389/fvets.2022.983482
    Database MEDical Literature Analysis and Retrieval System OnLINE

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